Assessing sperm membrane viability using flow cytometry in farmed New Zealand giant kokopu Galaxias argenteus

Abstract

We employed flow cytometry to assess plasma membrane viability of sperm cells in farmed giant kokopu Galaxias argenteus , an endemic amphidromous New Zealand fish. Specifically, the variables of fish size (TL mm) and weight (grams) were assessed against sperm viability. We found no significant difference ( P G. argenteus . This study shows that flow cytometry is an effective, accurate and rapid method for analysing sperm viability in G. argenteus .

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