Journal Article
TCAF2 drives glioma cellular migratory/invasion properties through STAT3 signaling
Debo Yun; Jianshen Liang; Xuya Wang; Jikang Fan; Xisen Wang; Jiabo Li; Xiao Ren; Jie Liu; Xiude Ren; Hao Zhang; Guanjie Shang; Wenzhe Jin; Lei Chen; Tao Li; Chen Zhang; Shengping Yu; Xuejun Yang
Molecular and Cellular Biochemistry · Vol. 479, Issue 7 · pp. 1801-1815 · 2024
Abstract
Glioma is an intracranial tumor characterized by high mortality and recurrence rates. In the present study, the association of TRPM8 channel-associated factor 2 (TCAF2) in glioma was investigated using bioinformatics, showing significant relationships with age, WHO grade, IDH, and 1p/19q status, as well as being an independent predictor of prognosis. Immunohistochemistry of a glioma sample microarray showed markedly increased TCAF2 expression in glioblastoma relative to lower-grade glioma, with elevated expression predominating in the tumor center. Raised TCAF2 levels promote glioma cell migratory/invasion properties through the epithelial-to-mesenchymal transition-like (EMT-like) process, shown by Transwell and scratch assays and western blotting. It was further found that the effects of TCAF2 were mediated by the activation of STAT3. These results suggest that TCAF2 promotes glioma cell migration and invasion, rendering it a potential drug target in glioma therapy.