Abstract
Effective conservation and enforcement strategies for marine turtles depend on understanding population structure, connectivity, and the geographic origins of turtles impacted by threats such as bycatch, overexploitation, and illegal trade. However, mitochondrial DNA (mtDNA) data remain fragmented across studies, with inconsistent sequence lengths, haplotype nomenclature, and metadata standards limiting their application in conservation, management, and wildlife forensics. To address these challenges, we developed ShellBank, a global marine turtle genetic traceability toolkit and open-access mtDNA database that consolidates and standardizes more than 20,000 mtDNA control-region sequences from published literature and verified contributed datasets. The platform harmonizes sequence lengths, haplotype nomenclature, and associated metadata, and provides tools to search haplotypes, access population-level baselines, extract frequency tables for Mixed Stock Analysis (MSA), and explore connectivity among nesting, in-water, and trade datasets. We demonstrate its utility through two case studies. First, mtDNA from a tortoiseshell seizure in the United Kingdom was compared against the ShellBank baseline, identifying likely source rookeries in the Eastern Caribbean and highlighting priority sampling gaps. Second, reanalysis of a hawksbill turtle foraging aggregation on the Great Barrier Reef using an expanded reference baseline substantially altered previous stock assignment estimates, corroborated flipper-tag and satellite-tracking data, and confirmed multi-stock connectivity across the western Pacific. By centralizing and standardizing global marine turtle mtDNA data, ShellBank improves the resolution, accuracy, transparency, and application of genetic traceability for conservation, management, and enforcement, strengthening the ability of researchers, managers, decision-makers, and enforcement agencies to identify source regions, detect population-level impacts, understand ecological connectivity, and guide evidence-based conservation actions.