Journal Article
The effect of glutathione and mannitol on androgenesis in anther and isolated microspore cultures of rye (Secale cereale L.)
Kamil Zieliński; Monika Krzewska; Iwona Żur; Katarzyna Juzoń; Przemysław Kopeć; Anna Nowicka; Jana Moravčiková; Edyta Skrzypek; Ewa Dubas
Plant Cell, Tissue and Organ Culture (PCTOC) · Vol. 140, Issue 3 · pp. 577-592 · 2020
Abstract
Androgenic responsiveness in anther (AC) and isolated microspore cultures (MC) was analysed using 15 lines of Polish winter rye ( Secale cereale L.). The effect of low temperature (LT) alone or in combination with osmotic stress induced by mannitol treatment (MAN) and/or with reduced glutathione (GSH) on the effectiveness of the process was studied. Interestingly, each treatment had a different effect on microspore (mcs) vitality and capability to divide symmetrically. The first criterion for successful embryogenesis was to exceed the threshold number of at least 25% dividing microspores, which determined ‘embryogenic suspension culture’. In some configurations a spectacular effect was achieved, especially in lines highly recalcitrant to androgenesis induction. Relatively high effectiveness of androgenesis induction (up to 4.58 AS per 10 5 mcs per spike in MC and 21.29 AS per spike in AC) showed that the developed protocol with GSH and/or MAN tiller pre-treatments overcomes the genotypic barrier for androgenesis initiation in rye. Moreover, relatively high, spontaneous genome diploidization (55%) of regenerated plants demonstrated that the described protocols could be effectively integrated into conventional rye breeding programmes.