Abstract
Aims Specific root respiration (RR S ) is a key root trait, determining i.e. nutrient foraging and uptake efficiencies. However, a considerable uncertainty exists regarding the effects of storage time and conditions on RR S measurements. Methods Fine root CO 2 efflux rates of three plant types (tree seedling Carpinus betulus , legume Pisum sativum , grass Lolium perenne ) were measured as depending on storage time (30–1440 min post-rinsing) and conditions (i.e. attached to plant, warm and cold water storage, and storage under dry conditions). Results Short-term storage conditions (30 min) had a significant effect on measured RR S rates, in specific, RR S rates of all three species were significantly lower under dry storage. Irrespective of plant species or temperature, storage of excised roots in water did not affect RR S for 300 min,. RR S measurements remained stable for 1 day if roots were stored cold. Conclusions Our results have important implications on measurement routines of RR S —a generally understudied root trait. Henceforth it seems reasonable to collect roots in the field and transport them, hydrated but even uncooled, to the laboratory for subsequent measurements for at least 300 min post-rinsing.