Abstract
The rich and special biodiversity in mountain rivers is experiencing a continuous decline due to the influence of climate change and anthropogenic factors. To explore the alterations in the distribution of aquatic biodiversity in mountain rivers in the context of cascade dam development, the environmental DNA (eDNA) metabarcoding technique was used to examine the aquatic biodiversity of the Wujiang River, a representative mountain river with an 11-stage cascade dam. In this study, a comprehensive analysis of aquatic organisms in the Wujiang River mainstem was conducted, and 17 phyla and 38 classes were detected in the 36 water samples. The most abundant group is the benthic organisms, and the least abundant group is the amphibians. The results of the diversity analysis showed a clear longitudinal distribution pattern of aquatic organisms along the altitudinal gradient, with higher richness of aquatic organism communities the further to the lower reaches. Meanwhile, RDA analysis revealed that altitude, dissolved oxygen, reservoir length, and reservoir construction time were the key environmental factors influencing the distribution of aquatic organisms in the Wujiang River mainstem. The findings of this research also showed the applicability of the eDNA method in detecting aquatic biodiversity.