Journal Article
From Design to Single-Cell Cloning: A Complete RNP-Based CRISPR-Cas9 Protocol for Precision Gene Correction in Human iPSCs
Giacomo Roman; Knut H. Lauritzen; Barbora Smolkova; Runar Almaas; Per Morten Sandset; Benedicte Stavik; Gareth J. Sullivan; Maria E. Chollet
Stem Cell Reviews and Reports · 2026
Abstract
Summary This integrated end-to-end protocol provides a comprehensive and efficient workflow for the generation, screening and expansion of high-quality monoclonal iPSC lines after CRISPR-Cas9 editing.
Bibliographic Information
| Journal | Stem Cell Reviews and Reports |
|---|
| Publisher | Springer |
|---|
| Publication Date | 2026-08-31 |
|---|
| Publication Year | 2026 |
|---|
| Document Type | Journal Article |
|---|
| Print ISSN | 2629-3269 |
|---|
| eISSN | 2629-3277 |
|---|
| DOI | 10.1007/s12015-026-11221-6 |
|---|
Access Information
Full-text access depends on NARA's subscribed coverage and institutional access.