Abstract
Aquatic surveys for fish in large water bodies (e.g., Laurentian Great Lakes of North America) often require a flexible approach using multiple methods, surveying different depths, and sampling across seasons, especially when the target species is elusive in its natural habitat. The round goby (Neogobius melanostomus) is an invasive, bottom-dwelling fish inhabiting rocky areas of all five Great Lakes. While trawl surveys are typically used for abundance assessments, angling has been demonstrated as a means of supplementing surveys with additional data. Yet, round goby abundance and distribution is still not well described. Recently, with considerable success, scientists have explored sampling environmental DNA (eDNA) to complement traditional monitoring techniques for population abundance estimates, early detection of invasive species, and spawning or migration events. Therefore, we collected eDNA from water samples alongside bottom trawls and hook and line angling in Lakes Huron and Michigan to detect round goby. eDNA samples were analyzed by both droplet digital PCR (ddPCR) and quantitative PCR (qPCR) to maximize the likelihood of detection. Overall, round goby was captured in 23% of the trawls, but the eDNA based methods detected round goby in 74% and 66% of samples by ddPCR and qPCR, respectively, mostly in samples collected at