Unveiling the Microplastics Menace in Freshwater Fishes: Evidence from the Panjnad Barrage, South Punjab, Pakistan

Abstract

This study explored the prevalence and types of microplastic (MP) pollution in three fish species—Labeo rohita, Wallago attu, and Cirrhinus mrigala—collected from the Panjnad Barrage in South Punjab, Pakistan. MPs were analyzed from the gastrointestinal tracts (GITs), gills, and muscles of 90 fish samples. Advanced analytical techniques, including Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy/Energy-Dispersive X-Ray Spectroscopy (SEM/EDX), and Gas Chromatography/Mass Spectrometry (GC/MS), were deployed, confirming the polymeric composition and presence of various additives. Quantitative and qualitative analyses revealed significant variations in MP accumulation across tissues, with the GIT consistently showing the highest MP count, the gills having the highest concentration per gram, and the muscles exhibiting the lowest amount of accumulation. Wallago attu was found to have accumulated the highest concentration of microparticles among all three species due to its feeding habits and habitat. Fibers and fragments were the predominant types of MPs, with yellow and red being the most frequent colors. Polypropylene (PP) and polyethylene (PE) were the primary polymers identified, alongside other types like polyethylene terephthalate (PET) and polyvinyl chloride (PVC). The MP size distribution indicated that mid-sized particles (150–50 µm) were most abundant in the GIT and gills, while smaller particles (

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