Abstract
The endangered freshwater fish Gobiobotia brevibarba is endemic to Korea and threatened by habitat disturbance in major river systems. We investigated four wild populations from the Han River basin (IJR, BHR, NHR) and the Geum River basin (GR) using eleven microsatellite loci to assess genetic diversity, population structure, and contemporary gene flow. All populations showed relatively high genetic diversity (HO = 0.709–0.800, HE = 0.707–0.803) and no evidence of inbreeding, although bottleneck signals under the infinite allele mutation model were detected in IJR and BHR. Contemporary effective population size was large in IJR (Ne = 2463) and moderate in NHR (Ne = 467), whereas estimates for BHR and GR were imprecise. Genetic differentiation was very low within the Han River basin (FST = 0.009–0.027) but weak and significant between Han and Geum (FST = 0.085–0.096), and clustering analyses (STRUCTURE, DAPC, find.cluster) consistently supported K = 2, separating Han from Geum River. Gene flow analyses indicated extremely limited interbasin gene flow (