Journal Article
Evaluation of Hypoxia Tolerance and Cloning-Expression Analysis of Related Genes in Hybrid Fish Derived from Megalobrama amblycephala × Xenocypris davidi Bleeker
Zhong Tang; Xinxin Yu; Xin Chen; Junwen Liang; Yangyang Tu; Zhifeng Zhou; Faxian Yu; Chun Zhang; Siyu Fan; Min Tao
Fishes · Vol. 11, Issue 4 · pp. 233 · 2026
Abstract
The diploid distant hybrid (2nBY) derived from female blunt snout bream (Megalobrama amblycephal, BSB) × male Bleeker’s yellow tail (Xenocypris davidi Bleeker, YT). To investigate the hypoxia tolerance and the regulatory mechanisms of hypoxia-inducible factor-1α/2α (hif-1α/2α) in 2nBY, BSB, and YT, experiments consisting of 24 h of hypoxia treatment (DO = 2.0 ± 0.1 mg/L) followed by 6 h of reoxygenation were conducted. The loss of equilibrium critical oxygen pressure (LOEcrit), gill tissue structure, and antioxidant indices, as well as the full-length sequences and expression of hif-1α/2α in 2nBY, BSB, and YT, were compared. The results showed that the LOEcrit value of 2nBY was significantly lower than that of BSB but higher than that of YT (p