Encapsulated Amazonian Microalgae Reduce Ammonia and Improve Survival of Female Poecilia reticulata During Simulated Transport

Abstract

The accumulation of un-ionized ammonia (NH3) during the transport of live ornamental fish poses a critical threat to animal welfare and post-transport survival. This study evaluated the efficacy of calcium alginate-encapsulated native Amazonian microalgae, Ankistrodesmus sp., Chlorella sp., Scenedesmus sp., and Synechococcus sp., for NH3 bioremediation during a 15-day simulated transport of female Poecilia reticulata. Biometric endpoints were selected using a four-criterion framework, identifying specific growth rate by weight (SGRW), specific growth rate by length (SGRL), and Fulton’s condition factor (K) as essential non-redundant parameters. Time-weighted average (TWA) NH3 served as the primary dose variable for four-parameter log-logistic modeling fitted to biological replicate means (n = 15). Ankistrodesmus sp. and Scenedesmus sp. maintained 97.8% survival and restricted TWA NH3 to 0.036 and 0.047 mg/L, respectively, whereas the empty capsule control reached 6.7% survival and 0.150 mg/L TWA NH3. Kruskal–Wallis tests on biological replicate means confirmed significant treatment effects on all biometric endpoints (SGRW: H(4) = 13.50, ε2 = 0.950; SGRL: H(4) = 13.50, ε2 = 0.95; p

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