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Isotopic Analysis as a Potential Tool to Verify Feed Protein Sources for Aquacultured Species

Kelly Brandeau Campbell; Michael Tlusty; Frederic T. Barrows
Fishes · Vol. 11, Issue 6 · pp. 363 · 2026

Abstract

This study identified δ15N stable isotope ratios as a robust tracer for fishmeal inclusion in aquaculture feeds. δ15N and δ13C values from fish muscle samples derived from feeding trials with seven species (n = 3–5 fish per diet group) were evaluated (+/−0.1‰ for both δ15N and δ13C; ~1% relative to % N and % C) to verify whether the presence or absence of fishmeal (FM) in feeds could be detected. C and N isotopic data were also analyzed for feed in two of the trials. δ13C signatures did not differ consistently across diet groups for each species examined, with mean δ13C values for all species investigated being −20.2‰ ± 1.3. In contrast, a strong δ15N distinction was discerned between FM- and non-FM-fed fish for both muscle and feed samples, with FM-fed groups presenting higher values (p

Bibliographic Information

JournalFishes
PublisherMDPI
Publication Date2026-06-17
Publication Year2026
Volume11
Issue6
Pages363
Document TypeJournal Article
eISSN2410-3888
DOI10.3390/fishes11060363
SubjectFisheries; fish biology; aquaculture; aquatic ecology; fisheries management

Access Information

NARA Access CoverageOA / free full text
Journal Homepagehttps://www.mdpi.com/journal/fishes
Publisher PageOpen Publisher Page
This article is openly available from the publisher.