Interactive Toxic Impacts of Thermal Stress and Residual Chlorine on Growth, Nutritional Composition and Digestive Physiology in Meretrix meretrix

Abstract

Thermal elevation and residual chlorine pollution are two anthropogenic stressors that co-occur in estuarine waters affected by coastal power plant effluents. A 30-day exposure experiment was established with six treatment combinations comprising three temperatures (27 °C, 28 °C, and 30 °C) and two residual chlorine concentrations (0 mg/L and 0.02 mg/L) on Meretrix meretrix. Sampling was conducted on days 0, 10, 20 and 30 of the trial. The results indicated that elevated temperature (30 °C) significantly suppressed shell height and total body weight, while residual chlorine (0.02 mg/L) exerted time-dependent effects on shell length and width, with a significant interactive effect on shell length only at day 30. Crude lipid content declined with increasing temperature and residual chlorine exposure, whereas moisture and ash contents showed significant two-way interactions at all sampling time points. Intestinal amylase activity was continuously regulated by both stressors, with interactive responses on days 10 and 20, and was significantly suppressed under combined high temperature and residual chlorine stress. Collectively, we infer that combined exposure to elevated temperature and residual chlorine impairs shell growth, muscle biochemical composition and intestinal amylase activity of M. meretrix. Long-term experiments are required to further clarify the joint toxic effects of thermal stress and residual chlorine on the clam species.

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