Growth Rates and Specific Aminoacyl-tRNA Synthetases Activities in Clupea harengus Larvae

Abstract

Gaining robust in situ estimates of the growth rate of marine fish larvae is essential for understanding processes controlling year-class success and developing sustainable management strategies to maintain good environmental status. We measured the growth rate of Atlantic herring (Clupea harengus) larvae in the laboratory and compared it to the activity of aminoacyl-tRNA synthetases (AARS). Larvae were reared under controlled conditions for 20 days at three temperatures (7, 12, and 17 °C) using different prey concentrations (0.1, 0.3, and 2 prey·mL−1) of the copepod Acartia tonsa. The relationship between specific growth rates (SGR) and specific AARS activities was best described by a linear function—SGR = −0.1031 + 0.0017 · spAARS, r2 = 0.71, p < 0.05—when only larvae fed ad libitum were considered regardless of the temperature. When larvae fed with low concentrations of food were included in the analysis, the relationship was SGR = −0.0332 + 0.0010 · spAARS, r2 = 0.42, p < 0.05. This latter slope was rather low compared to other studies performed in zooplankton. We suggest protein degradation during the early life stages of fish as the cause of this low slope. We also studied SGR under food deprivation and the effect on specific AARS activities. We found rather high specific AARS activities in small individuals of early stages of fish, also suggesting protein degradation. Further research about protein degradation and turnover rates is needed in order to use AARS activity as a proxy for growth rates in field-caught larvae.

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