Journal Article
Evaluation of Anchovy Egg Density: A Study on Differences Between Vertical and Oblique Towing Samplers for Ichthyoplankton
Gualtiero Basilone; Rosalia Ferreri; Angelo Bonanno; Simona Genovese; Salvatore Aronica
Oceans · Vol. 6, Issue 2 · pp. 31 · 2025
Abstract
The use of multiple sampling instruments during ichthyoplankton surveys could require a significant amount of time for sample collection procedures; moreover, their use is highly dependent on weather conditions. During surveys aimed at the application of the Daily Egg Production Method (DEPM), two different kinds of sampler are employed: PairoVET and Bongo 40. The possibility of using only one of these samplers may allow for a reduction in the sampling time on board and the subsequent analyses; thus, a study was carried out to test the use of the Bongo 40 sampler alone (i.e., without PairoVET sampling) in DEPM application. Samples collected during five ichthyoplankton surveys (from 2007 to 2010 and in 2014) were analyzed to compare the efficiency in anchovy egg collection. Although the compared ichthyoplankton samplers provided differences in the collected number of eggs, as well as in the filtered volume, non-significant differences between them were recorded in the egg density. An ANCOVA revealed a significant relationship (F1,5 = 606.80; p < 0.001) between the density estimates from the two nets, but no differences were recorded among years (F1,5 = 0.99; p < 1). The slopes of the linear regressions for the two datasets were close to 1, suggesting a substantial equivalence of the two samplers in estimating the egg density. Finally, since only a few sampling stations showed densities higher than 20 ind. m−2, conversion equations were provided to estimate the PairoVET density from the Bongo 40 data at different density levels (i.e., 20 ind. m−2 or higher).