Abstract
Purpose Clinical use of endovascular interventions has increased in recent decades for treatment of peripheral artery disease (PAD); however, molecular imaging paradigms for monitoring inflammatory responses or adverse remodeling in targeted arteries have not been validated. Therefore, this study tested the utility of positron emission tomography (PET) imaging with fluorine-18 ( 18 F)-fluorodeoxyglucose (FDG) for in vivo detection of peripheral angioplasty-induced arterial remodeling. Methods Yorkshire pigs ( n = 8) underwent fluoroscopy-guided overdilation of the right femoral artery using a balloon catheter inflated 1.25–1.85X the arterial diameter. 18 F-FDG PET/CT imaging was performed 14 days post-angioplasty. Injured and control arteries were harvested immediately after imaging and sectioned for gamma counting ( 18 F-FDG uptake) and stained with hematoxylin and eosin (H&E), alpha-smooth muscle actin (α-SMA), and CD64 for evaluation of arterial thickness, smooth muscle cell area, and macrophage infiltration. Results In vivo PET/CT imaging detected a significant increase in 18 F-FDG uptake in injured versus control femoral arteries 14 days post-angioplasty ( p = 0.008) that was confirmed by ex vivo gamma counting of vessels ( p = 0.02). Ex vivo gamma counting of 18 F-FDG uptake demonstrated a significant association with in vivo 18 F-FDG uptake measured by PET/CT imaging ( r = 0.92; p = 0.004). Image analysis for H&E, α-SMA, and CD64 revealed significant relative increases in vessel wall thickness ( p p = 0.0001), and CD64 area ( p = 0.04) that were each significantly associated with ex vivo measures of 18 F-FDG uptake. Conclusion 18 F-FDG PET/CT quantifies remodeling characteristics associated with angioplasty-induced injury, thereby providing a potential in vivo paradigm for monitoring and testing of emerging devices for PAD treatment.