Abstract
Summary The effect of glucose on the formation of gas vesicles was investigated in Haloferax mediterranei and Hfx.volcanii transformants containing the mc‐ gvp gene cluster of Hfx. mediterranei (mc‐vac transformants). Increasing amounts of glucose in the medium resulted in a successive decrease in the amount of gas vesicles in both species, with a complete inhibition of their formation at glucose concentrations of > 70 mM in mc‐vac transformants, and 100 mM in Hfx. mediterranei . Maltose and sucrose imposed a similar inhibitory effect, whereas xylose, arabinose, lactose, pyruvate and 2‐deoxy‐glucose had no influence on the gas vesicle formation in mc‐vac transformants. The activities of the two mc‐vac promoters were strongly reduced in mc‐vac transformants grown in the presence of > 50 mM glucose. The gas vesicle overproducing ΔD transformant (lacking the repressing protein GvpD) also showed a glucose‐induced lack of gas vesicles, indicating that GvpD is not involved in the repression. The addition of glucose was useful to block gas vesicle formation at a certain stage during growth, and vice versa , gas vesicle synthesis could be induced when a glucose‐grown culture was shifted to medium lacking glucose. Both procedures will enable the investigation of defined stages during gas vesicle formation.