Journal Article
A novel interdomain consortium from a Costa Rican oil well composed of Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov.
Linda Dengler; Julia Meier; Andreas Klingl; Laura Nißl; Annett Bellack; Dina Grohmann; Reinhard Rachel; Harald Huber
Archives of Microbiology · Vol. 205, Issue 5 · 2023
Abstract
A novel interdomain consortium composed of a methanogenic Archaeon and a sulfate-reducing bacterium was isolated from a microbial biofilm in an oil well in Cahuita National Park, Costa Rica. Both organisms can be grown in pure culture or as stable co-culture. The methanogenic cells were non-motile rods producing CH 4 exclusively from H 2 /CO 2 . Cells of the sulfate-reducing partner were motile rods forming cell aggregates. They utilized hydrogen, lactate, formate, and pyruvate as electron donors. Electron acceptors were sulfate, thiosulfate, and sulfite. 16S rRNA sequencing revealed 99% gene sequence similarity of strain CaP3V-M-L2A T to Methanobacterium subterraneum and 98.5% of strain CaP3V-S-L1A T to Desulfomicrobium baculatum . Both strains grew from 20 to 42 °C, pH 5.0–7.5, and 0–4% NaCl. Based on our data, type strains CaP3V-M-L2A T (= DSM 113354 T = JCM 39174 T ) and CaP3V-S-L1A T (= DSM 113299 T = JCM 39179 T ) represent novel species which we name Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov.