Abstract
Summary In our previous study, we found that pumilacidin‐like cyclic lipopeptides (CLPs) derived from marine bacterium Bacillus sp. strain 176 significantly suppressed the mobile capability and virulence of Vibrio alginolyticus . Here, to further disclose the mechanism of CLPs inhibiting the motility of V . alginolyticus , we first applied transcriptomic analysis to V . alginolyticus treated with or without CLPs. The transcriptomic results showed that the expression of several important components of the Na + ‐driven flagellar motor closely related to bacterial motility were markedly suppressed, suggesting that the structure and function of Na + ‐driven flagellar motor might be disabled by CLPs. The transcriptomic data were further analysed by the protein–protein interaction network, and the results supported that MotX, one of the essential components of Na + ‐driven flagellar motor was most likely the action target of CLPs. In combination of gene knockout, electrophoretic mobility shift assay and immunoblotting techniques, CLPs were demonstrated to affect the rotation of flagella of Vibrio alginolyticus via direct interacting with the Na + ‐driven flagellar motor component MotX, which eventually inhibited the bacterial motility. Interestingly, homologues of MotX were found broadly distributed and highly conserved in different pathogenic species, which extends the application range of CLPs as an antibacterial drug targeting bacterial motility in many pathogens.