Isogenic lines in fish – a critical review

Abstract

An isogenic line is a group of animals with an identical genotype, and its use is widely accepted for bioresearch standardization. Isogenic lines in fish can be generated within two generations via uniparental inheritance and can facilitate studies for which standardization and consistency are required. The availability and use of isogenic lines is limited, but isogenic lines in some fish species have been generated. Their power has been demonstrated in fields including human disease modelling, drug development, toxicology and also sequencing projects. The genetic basis of desired traits in aquaculture species can be identified using isogenic lines, which, in turn, will help to improve fish culture. This review summarized knowledge regarding the present status of isogenic lines in fish including approaches for their generation and verification, as well as challenges and potential applications for basic research and aquaculture.

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