Journal Article
Complete genome sequence of a novel potyvirus infecting Miscanthus sinensis (silver grass)
Zacharie Leblanc; Marie-Emilie Gauthier; Ruvini Lelwala; Candace Elliott; Cassie McMaster; Robin Eichner; Kevin Davis; Lia Liefting; Jeremy Thompson; Adrian Dinsdale; Mark Whattam; Julie Pattemore; Roberto A. Barrero
Archives of Virology · Vol. 167, Issue 8 · pp. 1701-1705 · 2022
Abstract
Here, we describe the full-length genome sequence of a novel potyvirus, tentatively named “Miscanthus sinensis mosaic virus” (MsiMV), isolated from Miscanthus sinensis (silver grass) held in a post-entry quarantine facility after being imported into Western Australia, Australia. The MsiMV genome is 9604 nucleotides (nt) in length, encoding a 3071-amino-acid (aa) polyprotein with conserved sequence motifs. The MsiMV genome is most closely related to that of sorghum mosaic virus (SrMV), with 74% nt and 78.5% aa sequence identity to the SrMV polyprotein region. Phylogenetic analysis based on the polyprotein grouped MsiMV with SrMV, sugarcane mosaic virus (SCMV), and maize dwarf mosaic virus (MDMV). This is the first report of a novel monopartite ssRNA virus in Miscanthus sinensis related to members of the genus Potyvirus in the family Potyviridae .