Journal Article
Genome sequence of pineapple secovirus B, a second sadwavirus reported infecting Ananas comosus
Adriana Larrea-Sarmiento; Andrew D.W. Geering; Alejandro Olmedo-Velarde; Xupeng Wang; Wayne Borth; Tracie K Matsumoto; Jon Y Suzuki; Marisa M Wall; Michael Melzer; Richard Moyle; Murray Sharman; John Hu; John E. Thomas
Archives of Virology · Vol. 167, Issue 12 · pp. 2801-2804 · 2022
Abstract
The complete genome sequence of pineapple secovirus B (PSV-B), a new virus infecting pineapple ( Ananas comosus ) on the island of Oahu, Hawaii, was determined by high-throughput sequencing (HTS). The genome comprises two RNAs that are 5,956 and 3,808 nt long, excluding the 3’-end poly-A tails, both coding for a single large polyprotein. The RNA1 polyprotein contains five conserved domains associated with replication, while the RNA2 polyprotein is cleaved into the movement protein and coat protein. PSV-B is representative of a new species in the subgenus Cholivirus (genus Sadwavirus ; family Secoviridae ), as the level of amino acid sequence identity to recognized members of this subgenus in the Pro-Pol and coat protein regions is below currently valid species demarcation thresholds.