Abstract
Plants can be used to recombinantly produce high-quality biopharmaceuticals, such as therapeutic proteins and vaccines, also known as plant molecular farming (PMF). Recent advances in engineering places PMF amongst the most innovative manufacturing technologies that reduce cost and shorten the production timeline. State-of-the-art PMF uses a dual-approach strategy to optimize the generation of products, namely engineering of expression vectors and production hosts. Here we describe the assembly of genetic circuits, engineered to enable the rapid expression of multimeric proteins in the commonly used expression host Nicotiana benthamiana .