We combine tissue expansion and light sheet fluorescence microscopy to analyze brain organoids. It is possible to zoom from a mesoscopic overview to super-resolution in a single imaging session, revealing cellular and subcellular structural details, such as the positions and spacing of pre- and postsynaptic proteins. Light sheet fluorescence expansion microscopy (LSFEM) greatly facilitates the qualitative and quantitative use of organoids in developmental and disease-related studies.
| Journal | BIOspektrum |
|---|---|
| Publisher | Springer |
| Publication Date | 2023-11-01 |
| Publication Year | 2023 |
| Volume | 29 |
| Issue | 7 |
| Pages | 740-744 |
| Document Type | Journal Article |
| Print ISSN | 0947-0867 |
| eISSN | 1868-6249 |
| DOI | 10.1007/s12268-023-2055-z |
| NARA Access Coverage | 2011-01-01~Current |
|---|---|
| Journal Homepage | https://www.springer.com/journal/12268 |
| Publisher Page | Open Publisher Page |