Abstract
Mechanosensation plays a key role in many physiological processes. Despite its importance, high-content methods for studying mechanosignaling at the cell level remain challenging. This article presents an approach to assess mechanosignaling in adherent cells using isotropic mechanical stretch combined with real-time Ca 2+ fluorescence imaging. This method integrates our IsoStretcher platform with image registration, cell segmentation, and single-cell and population-based Ca 2+ signaling analysis.