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Journal Article

Genome-wide characterization of simple sequence repeats in Palmae genomes

Manee M. Manee; Badr M. Al-Shomrani; Mohamed B. Al-Fageeh
Genes & Genomics · Vol. 42, Issue 5 · pp. 597-608 · 2020

Abstract

Background Microsatellites or simple sequence repeats (SSRs) have become the most significant DNA marker technology used in genetic research. The availability of complete draft genomes for a number of Palmae species has made it possible to perform genome-wide analysis of SSRs in these species. Palm trees are tropical and subtropical plants with agricultural and economic importance due to the nutritional value of their fruit cultivars. Objective This is the first comprehensive study examining and comparing microsatellites in completely-sequenced draft genomes of Palmae species. Methods We identified and compared perfect SSRs with 1–6 bp nucleotide motifs to characterize microsatellites in Palmae species using v0.2.5. We analyzed their relative abundance, relative density, and GC content in five palm species: Phoenix dactylifera , Cocos nucifera , Calamus simplicifolius , Elaeis oleifera , and Elaeis guineensis . Results A total of 118241, 328189, 450753, 176608, and 70694 SSRs were identified, respectively. The six repeat types were not evenly distributed across the five genomes. Mono- and dinucleotide SSRs were the most abundant, and GC content was highest in tri- and hexanucleotide SSRs. Conclusion We envisage that this analysis would further substantiate more in-depth computational, biochemical, and molecular studies on the roles SSRs may play in the genome organization of the palm species. The current study contributes a detailed characterization of simple sequence repeats in palm genomes.

Bibliographic Information

JournalGenes & Genomics
PublisherSpringer
Publication Date2020-05-01
Publication Year2020
Volume42
Issue5
Pages597-608
Document TypeJournal Article
Print ISSN1976-9571
eISSN2092-9293
DOI10.1007/s13258-020-00924-w

Access Information

NARA Access Coverage2009-01-01~Current
Journal Homepagehttps://www.springer.com/journal/13258
Publisher PageOpen Publisher Page
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