Journal Article
Shoot organogenesis and plant regeneration from leaf and petiole explants of Corydalis saxicola Bunting
Jinhui Pang; Yuping Xiong; Yujie Zeng; Xiaohong Chen; Xinhua Zhang; Yuan Li; Kunlin Wu; Songjun Zeng; Jaime A. Teixeira da Silva; Guohua Ma
In Vitro Cellular & Developmental Biology - Plant · Vol. 59, Issue 1 · pp. 121-128 · 2023
Abstract
Corydalis saxicola Bunting, a medicinal herb known for alkaloids, grows mainly in Guizhou province and northwest Guangxi province of China. In this study, for the first time for this plant, an efficient adventitious shoot proliferation and regeneration system via shoot organogenesis from leaf and petiole explants was established. Cytokinins, including 6-benzyladenine (BA) and thidiazuron (TDZ), induced callus and then shoot organogenesis. Most adventitious shoots were induced from leaf explants on Murashige and Skoog (MS) medium (pH 6.5) supplemented with 2.0 µM BA and 0.5 µM α-naphthaleneacetic acid (NAA). This medium was also optimal for shoot proliferation. The rooting ½MS medium was supplemented with 1.0 µM indole-3-butyric acid (IBA) and 0.2 µM NAA; 95.7% of shoots induced roots within 30 d and 91% of plantlets survived on a peat:vermiculite (1:1, v:v) substrate after 90 d.