Journal Article
Pacific Rim Forestry / Silviculture and Forest Ecology
Vishal Singh Negi; Archana Pal; Ratnesh Singh; Dulal Borthakur; Archana Pala; Vishal Singh Negi; Dulal Borthakur; Isabel Rushanaedy; Tyler Jones; Nicklos Dudley; Dulal Borthakur; Jannai Yafuso; Vishal Singh Negi; Dulal Borthakur; Kathleen Friday; Jacob Sanney; Maxson Nithan; Rickson Jonathan; Erick Waguk; Kathleen Friday; Uelbil Puanani Michael; Omekrael Sadang; Kathleen Friday; Eugene Eperiam; Wendolin Roseo Marquez; Kathleen Friday; Fa'afa'i Tony Maugalei; John Ah Sue; Kathleen Friday; Tyler Jones; Nicklos Dudley; Brian Strom; Sheri Smith; Joseph Fischer; Tyler Jones; Nicklos Dudley; Dulal Borthakur; Aileen Yeh; Richard Sniezko; Philip Cannon; Robert James; Susan Schenck; Erin Raboin; M. Tracy Johnson; Julie Denslow; Zhanfeng Liu; Neil Gurr; Mark Schmaedick; Arthur Whistler; Lisa Fischer; Tyler Jones; Nicklos Dudley; Robert James; Dulal Borthakur; Philip Cannon; Richard Sniezko; Susan Schenck; Randy Kolka; Paul Hanson; Rich Norby; Brian Palik; Stan Wullschleger; Charles Garten; Steve Sebestyen; Peter Thornton; John Bradford; Pat Mulhollend; Don Todd; Colleen Iversen; Jeff Warren; Kristin Peterson; Ayami Shiraishi; Janice Uchida; Michelle Tacconelli; Edward Loewenstein; Conor Bell; Kenneth Kettler; Michael Premer; Kara Oikarinen; Eric Dunnack; James Pickens; Marilynn Burkowski; Rico Gazal; Sean Dugan; Brian Oswald; Randy Balice; Daniel Unger; Melissa Kreye; Taylor Stein; Holly Ober; Leda Kobziar; Kimberly Bohn; Douglas Carter; Nancy Peterson; Paul Doruska; Giorgio Vacchiano; Roberta Berretti; Renzo Motta; Christy Michaels; Brian Oswald; Hans Williams; Kenneth Farrish; Jamie Schuler; Creighton Litton; Christian Giardina; Susan Crow; Lucas Fortini; Emma Schultz; Robert Seymour; Aaron Weiskittel; Christine Droske; Penelope Morgan; Anthony S. Davis; Peter R. Robichaud; Sarah Lewis; Andrew T. Hudak; Leigh B. Lentile; Paul Francis; Blair Orr; Michael Long; Creighton Litton; Christian Giardina; Jed Sparks; Thomas Macy; Charles Goebel; David Hix; David Miller; Mark Rudnicki; April Hiscox; Hong Bing Su; Thomas Kolb; Wilfred Previant; Linda Nagel; Christopher Webster; Angela Fuller; Daniel Harrison; Justin DeRose; John Shaw; Jim Long; Barbara Bentz; Eric Holzmueller; David Gibson; Paul Suchecki; Chris Stuhlinger; Emily Silver; Anthony D'Amato; Shawn Fraver; Brian Palik; Shane Strommer; Andrew Teets; Craig Houghton; Andrew Gundlach; Brian Fritz; Elizabeth Brantley; Chris Stuhlinger; Kyle Cunningham; Matthew Pelkki; Benjamin Caldwell; Antonin Kusbach; John Shaw; Jim Long; Nik Eiche; Brian Strom; Sheri Smith; Dryw Jones; Nicole Lightle; Scott Heckathorn; Nathaniel Morton; Brad Weigle; Kathryn McGown; Joshua Miller; Jane Stewart; Amy Ross-Davis; Anthony S. Davis; Sophan Chhin; Cheikh Mbow; Bienvenu Sambou; David Skole; Kurt Gottschalk; Gary Miller; Patrick Brose; Grant Tolley; Mike Battaglia; Theresa Jain; Han-Sup Han; Russell Graham; Christopher Keyes; Jeremy Fried
Journal of Forestry · Vol. 109, Issue 8 · pp. 557-570 · 2011
Abstract
Leucaena leucocephala (leucaena) is an important agroforestry tree of tropics and subtropics due to its protein rich foliages, ability to grow on poor soils, and high level of tolerance to various stresses including drought and diseases. We hypothesized that leucaena have a unique set of genes which confer tolerance to various abiotic and biotic stresses and these genes are either absent or not expressed in other legumes that are susceptible to these stress conditions. Interspecies suppression subtractive hybridization (iSSH) was performed to identify some unique genes from leucaena using cDNAs from leucaena and Acacia confusa (acacia) as the tester and driver, respectively. We identified 100 leucaena genes showing homology to various plant genes with known functions, of which 23 genes had homology to various stress-related proteins including chitinase, PR-10 protein, wound-stress protein, type-2 metallothionein, thaumatin-like protein, disease resistance response protein, cysteine proteinase, and kunitz trypsin inhibitor. We also identified 50 leucaena genes which either had homology to hypothetical proteins (HyPs) or had no homology at all. Putative functions of 15 HyPs were predicted using conserved domain and PSI-BLAST analyses. Linear motif analyses identified 17 HyPs with MAPK recognition motif, which is involved in signaling pathways, including stress responses. This study identified 23 known and some putative unique stress-related genes from leucaena which may serve as a useful source of stress-tolerance genes for tree and crop improvement in the future.