Growth Promotion of Maize Exposed to Arsenic and Mercury with a Consortia of Rhizosphere Bacteria Isolated from Mining Tailings

Abstract

In this study we evaluated plant growth promotion (PGP) traits of bacteria associated with maize ( Zea mays L.) including Pseudomonas sp. (TL36), Staphylococcus sp. (TL49), Gottfriedia sp. (TL52), and Bacillus sp. (TL80), isolated from mining tailings, when exposed to mercury and arsenic. Initially, the response of the bacteria to the heavy metals studied were evaluated in in vitro assays, showing regular-moderate tolerance to arsenic and low tolerance to mercury. Also plant growth promotion traits of the bacteria were studied in in vitro assays in terms of production of indole-3-acetic acid (IAA), phosphate solubilization, siderophores and biofilm when exposed to arsenic and mercury. In addition, production of volatile organic compounds by the four bacteria were measured. In general, bacterial PGP traits were maintained when exposed to arsenic though some differences were observed between bacteria and individual PGP trait. On the other hand, all the PGP traits were strongly reduced when exposed to mercury for all bacteria. Also, the compatibility between the four bacteria was examined in vitro. From the abovementioned results two consortia were established based on the TL36 and TL80 (consortium 1) and, TL49 and TL52 consortium 2, which were tested for plant growth promotion after single or multiple inoculations in maize exposed to arsenic and mercury. Single and multiple inoculation, with consortium 1 with Pseudomonas sp. and Bacillus sp. increased maize plant growth similarly, while on the other hand, consortium 2 with Staphylococcus sp. and Gottfriedia sp. had no effect on maize plant growth. Despite the observed moderate tolerance to arsenic of bacteria in consortium 1, the observed plant growth promotion was mitigated in the presence of arsenic.

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