A multi-stage framework for validating targeted environmental DNA (eDNA) assays: from in silico design to in vitro and in situ testing for fisheries monitoring - Matthew R. Charron; Brooklynne M. L. Keber; Rina Guxholli; Steven Rogers; Jessie L. Ogden; Paige W. Breault; Arfa A. Khan; Robert H. Hanner; Daniel D. Heath; Scott A. Pavey; Margaret F. Docker (Environmental Biology of Fishes, 2026)