Molecular cloning, characterization and expression analysis of B cell translocation gene 1 in grass carp Ctenopharyngodon idella

Abstract

An expressed sequence tag (EST) of B cell translocation gene (BTG) 1 ( gcbtg1 ) was obtained from a grass carp Ctenopharyngodon idellus intestinal complementary (c)DNA library and the full‐length cDNA sequence was acquired by rapid amplification of cDNA ends (RACE) technology. The predicted Gcbtg1 protein contains the box A and box B motifs which characterized the BTG and transducer of ERBB2 (TOB) family. Multiple alignment analysis reveals that Gcbtg1 shares an overall identity of 65–94% with Gcbtg1s of other vertebrates. Real‐time quantitative PCR analysis reveals that the highest expression level of gcbtg1 was detected in liver and the lowest in muscle. Western blotting analysis indicates that the immunological cross‐reactivity occurs between C. idella and human Homo sapiens BTG1 protein. A 1008 bp 5′‐flanking region sequence was cloned and analysed.

Bibliographic Information