Journal Article
Reproductive isolation between sympatric Anguilla japonica and Anguilla marmorata
S. Chow; H. Kurogi; T. Yamamoto; T. Tomoda; N. Mochioka; F. Shirotori; T. Yoshinaga; D. Ambe; M. Okazaki; S. Nagai; T. Yanagimoto
Journal of Fish Biology · Vol. 91, Issue 5 · pp. 1517-1525 · 2017
Abstract
Species‐specific restriction fragment length polymorphism in the intron of the androgen receptor gene ( ar5 ) was found in glass to silver‐stage individuals of Anguilla japonica ( n = 51) and A. marmorata ( n = 21). The sequence analysis of 16S rDNA from 328 anguillid leptocephali collected in the North Equatorial Current of the western North Pacific Ocean revealed the specimens to be A. japonica ( n = 194), A. marmorata ( n = 128), A. bicolor pacifica ( n = 5) and A. luzonensis ( n = 1). All leptocephali of A. japonica and A. marmorata were monomorphic and did not share an allele at the ar5 locus, indicating that the two species are reproductively isolated.