Abstract
We examined the reproductive life history of calico surfperch ( Amphistichus koelzi ), including mating season, pregnancy, gestation and multiple paternity utilizing restriction site‐associated DNA sequencing. Furthermore, we compared the mating season of calico with barred ( Amphistichus argenteus ) , walleye ( Hyperprosopon argenteum ) and silver ( Hyperprosopon ellipticum ) surfperches to determine if the timing of reproduction is divergent within and between the genera. In calico surfperch, the mating season occurs from October to November, and females gestate from December to May. All broods exhibit multiple paternity with a range of four to seven sires per brood. The mating season of calico overlaps completely with barred surfperch; however, barred surfperches have a protracted mating season which extends until the beginning of December, which may be due to differences in reproductive strategy such as size at first reproduction. In the genus, the Hyperprosopon mating season begins earlier than Amphistichus , with divergence in the onset of mating between Hyperprosopon congeners of approximately 1 month.