Construction of a single nucleotide polymorphism marker based QTL map and validation of resistance loci to bacterial wilt caused by Ralstonia solanacearum species complex in tomato

Abstract

Bacterial wilt (BW), caused by Ralstonia solanacearum species complex is one of the major biotic factors limiting tomato production in the humid tropics. Pyramiding of resistance genes through marker-assisted selection is an efficient way to develop durable BW resistant cultivars. Tomato line ‘Hawaii 7996’ (H7996) is a stable and robust resistance source against various strains of the species complex. Major BW resistance quantitative trait loci (QTLs) Bwr - 12 and Bwr - 6 , and several minor or strain specific QTLs have been coarse-mapped in this line, but none has been fine-mapped and validated. The objective of the current study was to construct a high density genetic map using single-nucleotide polymorphism (SNP) markers derived from genotyping-by-sequencing, fine-map Bwr - 12 and Bwr - 6 and determine the effects of these QTLs using a near isogenic line (NIL) population. A high density genetic map using 1604 SNP markers with an average distance of 0.82 cM was developed for 188 F 9 recombinant inbred lines derived from the cross H7996 × WVa700. A total of seven QTLs associated with BW resistance to race 1-phylotype I ( R. pseudosolanacearum ) or/and race 3-phylotype II ( R. solanacearum ) strains were located on chromosomes 6 ( Bwr - 6.1 , 6.2 , 6.3 and 6.4 ) and 12 ( Bwr - 12.1 , Bwr - 12.2 and Bwr - 12.3 ) with logarithm of odds (LOD) scores of 6.2–15.6 and 6.2–31.1, explaining 14.2–33.4% and 15.9–53.9% of the total phenotypic variation contributed from H7996, respectively. To validate the genetic effects of the two QTL regions, a set of 80 BC 3 F 3 NILs containing different sections of Bwr -6 with or without Bwr -12 was phenotyped for disease severity after challenge with either race 1-phylotype I Pss4 or race 3-phylotype II Pss1632 BW strains over two seasons. Bwr -6.1 specific to Pss4 and Bwr -6.3 specific to Pss1632 were mapped to an interval of 5.0 cM ( P < 0.05) between 6_33,444,000_SLM6-47 and 6_33,868,000_SLM6-124 SNP marker, and to 2.7 cM ( P < 0.01) between positions 6_35,949,000 _SLM6-107 to 6_36,750,000_SLM6-82 marker, respectively. In addition, the specific effect of Bwr -12 for resistance to Pss4 (LOD score of 5.8-16.1, P < 0.01) was confirmed.

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