Abstract
A previous study in our laboratory revealed that microRNA‐33 (miR‐33) regulated autophagy initiation and inflammatory response by targeting Atg5 ; furthermore, in this study, chloroquine (CQ), lipopolysaccharide (LPS) and the miR‐33 inhibitor were transfected into Ctenopharyngodon idella kidney (CIK) cells to explore whether miR‐33 regulated late‐stage autophagy and inflammatory response induced by LPS. The results showed that CQ inhibited the fusion of autophagosome and lysosome and significantly increased the secretion of pro‐inflammatory cytokines ( p p beclin‐1 , atg5 , atg7 and atg12 did not recover after inhibiting miR‐33 ( p > 0.05). But the expression of tnf‐α , il‐6 , il‐1β , il‐8 and nf‐κb , as well as the secretion of TNF‐α, IL‐6, IL‐12 and IL‐1β, were significantly downregulated, and the activities of ALP, SOD and CAT were significantly increased ( p p tnf‐α , il‐6 , il‐1β , il‐8 and nf‐κb ; inhibiting the secretion of TNF‐α, IL‐6, IL‐12 and IL‐1β; and increasing the activities of ACP, ALP, SOD and CAT ( p