Journal Article
Evaluation of the APTIMA Combo 2™ kit for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in frozen semen
Thibaut Lutz; Robin Chautard; Maxime Lafontaine; Merve Genc; Arabella Touati; Sabine Pereyre; Olivia Peuchant; Cécile Bébéar; Fabien Garnier; Sébastien Hantz
European Journal of Clinical Microbiology & Infectious Diseases · Vol. 44, Issue 8 · pp. 2007-2011 · 2025
Abstract
Detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) in cryopreserved semen is crucial for screening sperm donors. The evaluation of the limit of detection (LOD) of the APTIMA Combo 2™ kit (Panther, Hologic) was performed on cryopreserved semen samples spiked with CT and NG at concentrations ranging from 1 to 106 IFU/mL or CFU/mL, respectively. The LOD was 102 IFU/mL for CT and 10 CFU/mL for NG in single infection or coinfection. An inhibitory effect of semen on amplification was highlighted. This study confirmed the performance of the APTIMA Combo 2™ kit for screening cryopreserved sperm samples before donation.
Bibliographic Information
| Journal | European Journal of Clinical Microbiology & Infectious Diseases |
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| Publisher | Springer |
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| Publication Date | 2025-08-01 |
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| Publication Year | 2025 |
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| Volume | 44 |
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| Issue | 8 |
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| Pages | 2007-2011 |
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| Document Type | Journal Article |
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| Print ISSN | 0934-9723 |
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| eISSN | 1435-4373 |
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| DOI | 10.1007/s10096-025-05138-0 |
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