Journal Article
Lytic activity of F8-like phage φSPM-1 against One Health lineages of carbapenem-resistant Pseudomonas aeruginosa
Patrícia R. Neves; Karine Dantas; Gregory Melocco; Helena Turano; Fernanda Esposito; Ana C. Gales; Carlo E. Levy; Elsa M. Mamizuka; Nilton Lincopan
European Journal of Clinical Microbiology & Infectious Diseases · 2026
Abstract
The increasing resistance of Pseudomonas aeruginosa to carbapenem antibiotics is shifting the landscape of high-risk clones from a strictly hospital-acquired threat to a global pathogen with One Health relevance. Given the limited therapeutic options available, developing new treatments is essential. We previously reported the complete genome sequence of φSPM-1 phage from the family Myoviridae . In this study, we show that φSPM-1 displays lytic activity against hospital and environmental metallo-β-lactamase (MβL)-producing P. aeruginosa and confers a protective effect against lethal infection caused by the high-risk clone ST277 in a Galleria mellonella prophylactic model.