Biofilm recovery in a wastewater treatment plant‐influenced stream and spatial segregation of ammonia‐oxidizing microbial populations

Abstract

We monitored the effects of wastewater treatment plant (WWTP) inputs on the recovery of stream biofilms after a large flood event that eroded most of the former biofilm communities. We monitored biomass recovery, chlorophyll a , nitrogen content, and stable isotope natural abundance ( 15 N) over 8 weeks in light‐ and dark‐exposed biofilms upstream and downstream from WWTP inputs, respectively, as well as the abundance of ammonia oxidizers by quantitative polymerase chain reaction. Biomass and chlorophyll a recovered quickly ( 15 N signature increased as the flood receded. Biofilm 15 N downstream of WWTP increased over time, tracking the increase in 15 N‐ammonium from the WWTP waters. Bacterial and archaeal ammonia oxidizers were present within the biofilm assemblages from early stages of postflood recovery. However, spatial distribution of these two clades was clearly segregated among sites and between light‐ and dark‐exposed biofilms, probably related to ammonium availability and the development of photoautotrophic organisms.

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