Journal Article
Localization of QTL for cold tolerance at the germination stage of rice (Oryza sativa L.) using recombinant inbred lines
Liangzi Cao; Shukun Jiang; Guohua Ding; Tongtong Wang; Liangming Bai; Jinsong Zhou; Yu Luo; Tianshu Xia; Hui Jiang; Kai Liu; Xueyang Wang; Guang Yang; Shichen Sun
Genetic Resources and Crop Evolution · Vol. 69, Issue 4 · pp. 1649-1660 · 2022
Abstract
The cold tolerance of germinating direct-sown rice ( Oryza sativa L.) has an increased rate of emergence, which ensures vigorous seedling growth. Research on QTL localization for cold tolerance at the germination stage can assist in molecular marker-assisted selection and enhance breeding efficiency. In this study, 94 populations of recombinant self-incompatible lines from Heigu and Ha 9366 were selected to investigate germination rates at low temperatures. It was found that two QTL loci (qLTG-3 and qLTG-12) were located at different germination times on chromosomes 3 and 12, respectively. The two QTLs at three different germination times, located using QTL, accounted for 21.3–25.9% of the phenotypic variation. Moreover, a reciprocal effect was detected between the two QTLs. The double QTLs increased the germination rate by 22–27% in this population. Additionally, qLTG-12 improved cold tolerance at the seedling stage. The results of this study might provide the materials and molecular markers for future molecular marker-assisted breeding for cold tolerance at the germination stage.