Abstract
Microsatellite DNA markers were developed from a peregrine falcon ( Falco peregrinus ) and genetic relationships among peregrine falcons in southern Norway were analysed using the markers. The genomic DNA library was screened for the presence of dinucleotide microsatellite repeats. Twelve loci revealed polymorphism through the initial analysis of 24 unrelated peregrine falcons, and Mendelian inheritance was confirmed in two peregrine falcon families bred in captivity. The estimated mean probability of identical genotypes in two unrelated individuals was 3 × 10 −8 , and the combined exclusion probability for parentage testing was 0.99 and 0.94 for one or both parents unknown, respectively. The markers were used to investigate the parentage of peregrine broods from the same nest site from different breeding seasons, and subsequently the nest‐site fidelity of the breeding peregrines. High nest‐site fidelity was found by studying pairwise comparisons of relatedness ( r xy ) estimates among chicks at six nest sites from three different breeding seasons. Cross‐species amplifications showed that most loci also appeared to amplify polymorphic products in the gyrfalcon ( F. rusticolus ), merlin ( F. columbarius ), hobby ( F. subbuteo ) and kestrel ( F. tinnunculus ), demonstrating that the loci will provide powerful genetic markers in these falcons too.