Abstract
This study highlights the aqueous synthesis of CdTe/ZnS core/shell quantum dots (QDs) and their application as fluorescence sensors for detecting critical metabolites, including folic acid, glucose, and vitamin C, in real biological samples. The synthesized QDs exhibit excellent quantum efficiency, stability, and biocompatibility, enhanced by mercaptopropionic acid (MPA) ligands, enabling eco-friendly and accurate sensing. Detection limits of 0.84 µg/mL for folic acid, 0.33 mM for glucose, and 1.15 µg/mL for vitamin C were achieved with high linearity (R 2 > 0.97). These results underscore the potential of CdTe/ZnS QDs in advanced biosensing technologies, offering sensitive and selective metabolite detection through a robust FRET-based mechanism. The versatility and aqueous solubility of these QDs pave the way for their integration into multiplex diagnostic systems for enhanced biomedical applications.