NARA Discovery
Article Details
← Back to Search Results
Journal Article

Dual‐compartmental transcriptomic + proteomic analysis of a marine endosymbiosis exposed to environmental change

Anderson B. Mayfield; Yu‐Bin Wang; Chii‐Shiarng Chen; Shu‐Hwa Chen; Chung‐Yen Lin
Molecular Ecology · Vol. 25, Issue 23 · pp. 5944-5958 · 2016

Abstract

As significant anthropogenic pressures are putting undue stress on the world's oceans, there has been a concerted effort to understand how marine organisms respond to environmental change. Transcriptomic approaches, in particular, have been readily employed to document the m RNA ‐level response of a plethora of marine invertebrates exposed to an array of simulated stress scenarios, with the tacit and untested assumption being that the respective proteins show a corresponding trend. To better understand the degree of congruency between m RNA and protein expression in an endosymbiotic marine invertebrate, m RNA s and proteins were sequenced from the same samples of the common, Indo‐Pacific coral Seriatopora hystrix exposed to stable or upwelling‐simulating conditions for 1 week. Of the 167 proteins downregulated at variable temperature, only two were associated with m RNA s that were also differentially expressed between treatments. Of the 378 differentially expressed genes, none were associated with a differentially expressed protein. Collectively, these results highlight the inherent risk of inferring cellular behaviour based on m RNA expression data alone and challenge the current, m RNA ‐focused approach taken by most marine and many molecular biologists.

Bibliographic Information

JournalMolecular Ecology
PublisherWiley
Publication Date2016-12-01
Publication Year2016
Volume25
Issue23
Pages5944-5958
Document TypeJournal Article
Print ISSN0962-1083
eISSN1365-294X
DOI10.1111/mec.13896
SubjectEcology & Organismal Biology

Access Information

NARA Access Coverage1997-01-01~Current
Journal Homepagehttps://onlinelibrary.wiley.com/loi/1365294X
Publisher PageOpen Publisher Page
Full-text access depends on NARA's subscribed coverage and institutional access.