Trait-based approaches reveal that deep reef ecosystems in the Western Indian Ocean are functionally distinct - Paris V. Stefanoudis; Nico Fassbender; Kaveh Samimi-Namin; Pierre-André Adam; Ameer Ebrahim; Jerome Harlay; Anna Koester; Melita Samoilys; Helena Sims; Denise Swanborn; Sheena Talma; Sara Winter; et al. (Science of The Total Environment, 2023)

Equal use of Indigenous and scientific knowledge in species assessments: A case study from the Northwest Territories, Canada - Claire L. Singer; Mélanie R. Routh; Michele J. Grabke; Leon Andrew; Suzanne Carrière; Aimee Guile; Alestine Andre; Allison Thompson; Deborah Simmons; Kaytlin Cooper; Lynda Yonge; Moise Rabesca; et al. (Biological Conservation, 2023)

A Systematic Review of the Genetic Predisposition to Injury in Football - Alexander B. T. McAuley; David C. Hughes; Loukia G. Tsaprouni; Ian Varley; Bruce Suraci; Thomas R. Roos; Adam J. Herbert; Daniel T. Jackson; Adam L. Kelly (Journal of Science in Sport and Exercise, 2023)
Purpose Synthesise genetic association studies investigating injury involving football players to identify which genetic variants have the most empirical evidence to date. Methods A comprehensive search of the PubMed, SPORTDiscus, and MEDLINE databases until March 11th 2022 identified 34 studies. Inclusion criteria: primary investigations, included football players, examined the association of a genetic variant with injury, and were published in English. Risk of bias was assessed using the Newcastle–Ottawa Scale. A narrative synthesis summarised results. Results There were 33 candidate gene studies and one genome-wide study, with 9642 participants across all studies (range = 43–1311; median = 227). Ninety-nine polymorphisms were assessed within 63 genes. Forty-one polymorphisms were associated with injury once. Three polymorphisms had their specific allelic associations with injury replicated twice in independent cohorts: ACTN3 (rs1815739) XX genotype was associated with an increased susceptibility to non-contact muscle injuries, ACAN (rs1516797) G allele was associated with increased susceptibility to anterior cruciate ligament (ACL) injuries, and VEGFA (rs2010963) CC genotype was associated with an increased susceptibility to ACL and ligament or tendon injuries. However, several methodological issues (e.g., small sample sizes, cohort heterogeneity, and population stratification) are prevalent that limit the reliability and external validity of findings. Conclusion At present, the evidence base supporting the integration of genetic information as a prognostic or diagnosis tool for injury risk in football is weak. Future participation of organisations in international consortia is suggested to combat the current methodological issues and subsequently improve clarity concerning the underlying genetic contribution to injury susceptibility.

Fishing for hosts: Larval spurting by the endangered thick‐shelled river mussel, U nio c rassus - David C. Aldridge; Joshua I. Brian; Adam Ćmiel; Anna Lipińska; Manuel Lopes‐Lima; Ronaldo Sousa; Amilcar Teixeira; Katarzyna Zając; Tadeusz Zając (Ecology, 2023)

Superficial white matter across development, young adulthood, and aging: volume, thickness, and relationship with cortical features - Kurt G. Schilling; Derek Archer; Francois Rheault; Ilwoo Lyu; Yuankai Huo; Leon Y. Cai; Silvia A. Bunge; Kevin S. Weiner; John C. Gore; Adam W. Anderson; Bennett A. Landman (Brain Structure and Function, 2023)

Protectin DX as a therapeutic strategy against frailty in mice - Laís R. Perazza; Adam C. Gower; Holly M. Brown-Borg; Paola Divieti Pajevic; LaDora V. Thompson (GeroScience, 2023)
Frailty in aging is driven by the dysregulation of multiple biological pathways. Protectin DX (PDX) is a docosahexaenoic acid (DHA)-derived molecule that alleviates many chronic inflammatory disorders, but its potential effects on frailty remain unknown. Our goal is to identify age-related impairments in metabolic systems and to evaluate the therapeutic potential of PDX on frailty, physical performance, and health parameters. A set of 22-month-old C57BL/6 male and female mice were assigned to vehicle (Old) or PDX daily gavage treatment for 9 weeks, whereas 6-month-old (Adult) mice received only vehicle. Forelimb and hindlimb strength, endurance, voluntary wheel activity and walking speed determined physical performance and were combined with a frailty index score and body weight loss to determine frailty status. Our data shows that old vehicle-treated mice from both sexes had body weight loss paralleling visceromegaly, and Old females also had impaired insulin clearance as compared to the Adult group. Aging was associated with physical performance decline together with higher odds of frailty development. There was also age-driven mesangial expansion and glomerular hypertrophy as well as bone mineral density loss. All of the in vivo and in vitro impairments observed with aging co-occurred with upregulation of inflammatory pathways and Myc signaling as well as downregulation of genes related to adipogenesis and oxidative phosphorylation in liver. PDX attenuated the age-driven physical performance (strength, exhaustion, walking speed) decline, promoted robustness, prevented bone losses and partially reversed changes in hepatic expression of Myc targets and metabolic genes. In conclusion, our data provides evidence of the beneficial therapeutic effect of PDX against features of frailty in mice. Further studies are warranted to investigate the mechanisms of action and the potential for human translation.

Small-scale oxygen distribution patterns in a coral reef - Adam S. Candy; Shannara K. Taylor Parkins; Fleur C. Van Duyl; Benjamin Mueller; Milou G. I. Arts; Will Barnes; Marie Carstensen; Yun J. H. Scholten; Yusuf C. El-Khaled; Christian Wild; Linda Wegley Kelly; Craig E. Nelson; et al. (Frontiers in Marine Science, 2023)
One mechanism giving fleshy algae a competitive advantage over corals during reef degradation is algal-induced and microbially-mediated hypoxia (typically less than 69.5 µmol oxygen L −1 ). During hypoxic conditions oxygen availability becomes insufficient to sustain aerobic respiration in most metazoans. Algae are more tolerant of low oxygen conditions and may outcompete corals weakened by hypoxia. A key question on the ecological importance of this mechanism remains unanswered: How extensive are local hypoxic zones in highly turbulent aquatic environments, continuously flushed by currents and wave surge? To better understand the concert of biological, chemical, and physical factors that determine the abundance and distribution of oxygen in this environment, we combined 3D imagery, flow measurements, macro- and micro-organismal abundance estimates, and experimentally determined biogenic oxygen and carbon fluxes as input values for a 3D bio-physical model. The model was first developed and verified for controlled flume experiments containing coral and algal colonies in direct interaction. We then developed a three-dimensional numerical model of an existing coral reef plot off the coast of Curaçao where oxygen concentrations for comparison were collected in a small-scale grid using fiberoptic oxygen optodes. Oxygen distribution patterns given by the model were a good predictor for in situ concentrations and indicate widespread localized differences exceeding 50 µmol L -1 over distances less than a decimeter. This suggests that small-scale hypoxic zones can persist for an extended period of time in the turbulent environment of a wave- and surge- exposed coral reef. This work highlights how the combination of three-dimensional imagery, biogenic fluxes, and fluid dynamic modeling can provide a powerful tool to illustrate and predict the distribution of analytes (e.g., oxygen or other bioactive substances) in a highly complex system.

The transcription regulator ATF4 is a mediator of skeletal muscle aging - Matthew J. Miller; George R. Marcotte; Nathan Basisty; Cameron Wehrfritz; Zachary C. Ryan; Matthew D. Strub; Andrew T. McKeen; Jennifer I. Stern; Karl A. Nath; Blake B. Rasmussen; Andrew R. Judge; Birgit Schilling; et al. (GeroScience, 2023)
Aging slowly erodes skeletal muscle strength and mass, eventually leading to profound functional deficits and muscle atrophy. The molecular mechanisms of skeletal muscle aging are not well understood. To better understand mechanisms of muscle aging, we investigated the potential role of ATF4, a transcription regulatory protein that can rapidly promote skeletal muscle atrophy in young animals deprived of adequate nutrition or activity. To test the hypothesis that ATF4 may be involved in skeletal muscle aging, we studied fed and active muscle-specific ATF4 knockout mice (ATF4 mKO mice) at 6 months of age, when wild-type mice have achieved peak muscle mass and function, and at 22 months of age, when wild-type mice have begun to manifest age-related muscle atrophy and weakness. We found that 6-month-old ATF4 mKO mice develop normally and are phenotypically indistinguishable from 6-month-old littermate control mice. However, as ATF4 mKO mice become older, they exhibit significant protection from age-related declines in strength, muscle quality, exercise capacity, and muscle mass. Furthermore, ATF4 mKO muscles are protected from some of the transcriptional changes characteristic of normal muscle aging (repression of certain anabolic mRNAs and induction of certain senescence-associated mRNAs), and ATF4 mKO muscles exhibit altered turnover of several proteins with important roles in skeletal muscle structure and metabolism. Collectively, these data suggest ATF4 as an essential mediator of skeletal muscle aging and provide new insight into a degenerative process that impairs the health and quality of life of many older adults.

MOO-GAPS: A multi-objective optimization model for global animal production and sustainability - Adam C. Castonguay; Stephen Polasky; Matthew H. Holden; Mario Herrero; Jinfeng Chang; Daniel Mason-D’Croz; Cecile Godde; Katie Lee; Brett A. Bryan; James Gerber; Edward T. Game; Eve McDonald-Madden (Journal of Cleaner Production, 2023)

Links to rare climates do not translate into distinct traits for island endemics - Vanessa Cutts; Dagmar M. Hanz; Martha Paola Barajas‐Barbosa; Franziska Schrodt; Manuel J. Steinbauer; Carl Beierkuhnlein; Pierre Denelle; José María Fernández‐Palacios; Pierre Gaüzère; Matthias Grenié; Severin D. H. Irl; Nathan Kraft; et al. (Ecology Letters, 2023)
Current models of island biogeography treat endemic and non‐endemic species as if they were functionally equivalent, focussing primarily on species richness. Thus, the functional composition of island biotas in relation to island biogeographical variables remains largely unknown. Using plant trait data (plant height, leaf area and flower length) for 895 native species in the Canary Islands, we related functional trait distinctiveness and climate rarity for endemic and non‐endemic species and island ages. Endemics showed a link to climatically rare conditions that is consistent with island geological change through time. However, functional trait distinctiveness did not differ between endemics and non‐endemics and remained constant with island age. Thus, there is no obvious link between trait distinctiveness and occupancy of rare climates, at least for the traits measured here, suggesting that treating endemic and non‐endemic species as functionally equivalent in island biogeography is not fundamentally wrong.

Generation of the Chemical and Social Stressors Integration Technique (CASS-IT) to identify areas of holistic public health concern: An application to North Carolina - Lauren A. Eaves; Paul Lanier; Adam E. Enggasser; Gerard Chung; Toby Turla; Julia E. Rager; Rebecca C. Fry (Science of The Total Environment, 2023)

The association of auditory function measures with low-level methylmercury from oceanic fish consumption and mercury vapor from amalgam: The Seychelles Child Development Study Nutrition 1 Cohort - Mark S. Orlando; Tanzy Love; Donald Harrington; Adam C. Dziorny; Conrad F. Shamlaye; Gene E. Watson; Edwin van Wijngaarden; Philip W. Davidson; Gary J. Myers (NeuroToxicology, 2023)

Shifts in bowhead whale distribution, behavior, and condition following rapid sea ice change in the Bering sea - John J. Citta; Greg A. Breed; Stephen R. Okkonen; Matthew L. Druckenmiller; Lori Quakenbush; John C. George; Billy Adams; Wieslaw Maslowski; Robert Osinski; Justin Olnes; Ellen V. Lea; Robert Suydam (Continental Shelf Research, 2023)

Natural organic matter source, concentration, and pH influences the toxicity of zinc to a freshwater microalga - Gwilym A.V. Price; Jenny L. Stauber; Dianne F. Jolley; Darren J. Koppel; Eric J. Van Genderen; Adam C. Ryan; Aleicia Holland (Environmental Pollution, 2023)

Recommendations for improving the reporting and communication of aquatic toxicity studies for oil spill planning, response, and environmental assessment - Adriana C. Bejarano; Julie E. Adams; Judith McDowell; Thomas F. Parkerton; Mark L. Hanson (Aquatic Toxicology, 2023)

Constructed Wetlands Suitability for Sugarcane Profitability, Freshwater Biodiversity and Ecosystem Services - Adam D. Canning; James C. R. Smart; Joshua Dyke; Graeme Curwen; Syezlin Hasan; Nathan J. Waltham (Environmental Management, 2023)
Freshwater ecosystems, such as wetlands, are among the most impacted by agricultural expansion and intensification through extensive drainage and pollution. There is a pressing need to identify ways of managing agricultural landscapes to ensure food and water security without jeopardising biodiversity and other environmental benefits. Here we examine the potential fish biodiversity and landholder financial benefits arising from the integration of constructed lagoons to improve drainage, flow regulation and habitat connectivity within a sugarcane dominated catchment in north Queensland, Australia. A hybrid approach was used, combining the findings of both fish ecological surveys and a financial cost-benefit analysis. We found that the constructed lagoons supported at least 36 native freshwater fishes (over half of all native freshwater fishes in the region), owing to their depth, vegetated margins, moderate water quality and high connectivity to the Tully River. In addition to biodiversity benefits, we estimated that surrounding sugarcane farms would have financially benefited from reduced flooding of cropland and the elevation of low-lying cropland with deposited spoil excavated from lagoon construction. Improved drainage and flow regulation allowed for improvement in sugarcane yield and elevated land increased gross margins from extending the length of the cane production cycle or enabling a switch from cattle grazing to cane production. Restoring or creating wetlands to reduce flooding in flood-prone catchments is a globally applicable model that could improve both agricultural productivity and aquatic biodiversity, while potentially increasing farm income by attracting payments for provision of ecosystem services.

Correction to: Viruses of Atlantic Bonefsh (Albula vulpes) in Florida and the Caribbean show geographic patterns consistent with population declines - Lewis J. Campbell; Nicholas A. Castillo; Christopher D. Dunn; Addiel Perez; Juan J. Schmitter-Soto; Sahar C. Mejri; Ross E. Boucek; Rolando Santos Corujo; Aaron J. Adams; Jennifer S. Rehage; Tony L. Goldberg (Environmental Biology of Fishes, 2023)

Population dynamics and movements of Atlantic tarpon, Megalops atlanticus, in the Parnaíba Delta Protected Area, Brazil: challenges for local fishery management planning - Cezar A. F. Fernandes; Francisca E. A. Cunha; Carlos E. L. S. Silva; Alan C. S. Araújo; Rafael L. Pereira; Daniel F. Viana; Werlanne M. S. Magalhães; M. A. P. Gondolo; Daniel M. P. de Castro; Aaron Adams; Jiangang Luo; Jerald S. Ault; et al. (Environmental Biology of Fishes, 2023)

Viruses of Atlantic Bonefish (Albula vulpes) in Florida and the Caribbean show geographic patterns consistent with population declines - Lewis J. Campbell; Nicholas A. Castillo; Christopher D. Dunn; Addiel Perez; Juan J. Schmitter-Soto; Sahar C. Mejri; Ross E. Boucek; Rolando Santos Corujo; Aaron J. Adams; Jennifer S. Rehage; Tony L. Goldberg (Environmental Biology of Fishes, 2023)

Product Quality Research for Developing and Assessing Regulatory Submissions for Generic Cyclosporine Ophthalmic Emulsions - Avery Belenos; Erin Leigh Wood; Meng Hu; Darby Kozak; Xiaoming Xu; Adam C. Fisher (The AAPS Journal, 2023)
Approval of the first generic 0.05% cyclosporine ophthalmic emulsion (COE) in the U.S. represents a milestone achievement of the science and research program in the U.S. Food and Drug Administration’s Center for Drug Evaluation and Research (CDER). COE is a locally acting complex drug product indicated to increase tear production in patients whose production is presumed to be suppressed due to ocular inflammation associated with keratoconjunctivitis sicca. The path to approval required overcoming numerous scientific challenges to determining therapeutic equivalence to the reference listed drug. Researchers in CDER’s Office of Pharmaceutical Quality and Office of Generic Drugs developed a quality by design approach to understand the effects of process and formulation variables on the product’s critical quality attributes, including globule size distribution (GSD), turbidity, viscosity, zeta potential, surface tension, and osmolality. CDER researchers explored multiple techniques to perform physicochemical characterization and analyze the GSD including laser diffraction, nanoparticle tracking analysis, cryogenic transmission electron microscopy, dynamic light scattering, asymmetric field flow fractionation, and two-dimensional diffusion ordered spectroscopy nuclear magnetic resonance. Biphasic models to study drug transfer kinetics demonstrated that COEs with qualitative and quantitative sameness and comparable GSDs, analyzed using earth mover’s distance, can be therapeutic equivalents. This body of research facilitated the review and approval of the first U.S. generic COE. In addition, the methods and fundamental understanding developed from this research may support the development and assessment of other complex generics. The approval of a generic COE should improve the availability of this complex drug product to U.S. patients. Graphical Abstract

Polymorphism of the follicle stimulating hormone receptor does not impact reproductive performance or in-vitro embryo production in beef heifers - Alexandria P. Snider; Hannah K. Yake; Camryn D. Granger; Shelby L. Rosasco; Tara G. McDaneld; Warren M. Snelling; Chad C. Chase; Jeremy R. Miles; Clay A. Lents; Lacey K. Quail; Jerica J.J. Rich; Kaitlin M. Epperson; et al. (Theriogenology, 2023)

Biochar-mediated Anammox granular sludge: Formation/characteristics of pitaya type granular sludge and enhanced performance of Anammox process - Junxiang Xie; Tianyi An; Adams Mabruk; Yayi Wang; Tian C. Zhang; Jianfang Wang; Chongjun Chen (Journal of Cleaner Production, 2023)

Use of the head-twitch response to investigate the structure–activity relationships of 4-thio-substituted 2,5-dimethoxyphenylalkylamines - Adam L. Halberstadt; Dino Luethi; Marius C. Hoener; Daniel Trachsel; Simon D. Brandt; Matthias E. Liechti (Psychopharmacology, 2023)
Rationale 4-Thio-substituted phenylalkylamines such as 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) and 2,5-dimethoxy-4- n -propylthiophenethylamine (2C-T-7) produce psychedelic effects in humans and have been distributed as recreational drugs. Objectives The present studies were conducted to examine the structure–activity relationships (SAR) of a series of 4-thio-substituted phenylalkylamines using the head twitch response (HTR), a 5-HT 2A receptor-mediated behavior induced by psychedelic drugs in mice. The HTR is commonly used as a behavioral proxy in rodents for human psychedelic effects and can be used to discriminate hallucinogenic and non-hallucinogenic 5-HT 2A agonists. Methods HTR dose–response studies with twelve different 4-thio-substituted phenylalkylamines were conducted in male C57BL/6 J mice. To detect the HTR, head movement was recorded electronically using a magnetometer coil and then head twitches were identified in the recordings using a validated method based on artificial intelligence. Results 2C-T, the parent compound of this series, had relatively low potency in the HTR paradigm, but adding an α-methyl group increased potency fivefold. Potency was also increased when the 4-methylthio group was extended by one to three methylene units. Fluorination of the 4-position alkylthio chain, however, was detrimental for activity, as was the presence of a 4-allylthio substituent versus a propylthio group. 2C-T analogs containing a 4-benzylthio group showed little or no effect in the HTR paradigm, which is consistent with evidence that bulky 4-substituents can dampen agonist efficacy at the 5-HT 2A receptor. Binding and functional studies confirmed that the compounds have nanomolar affinity for 5-HT 2 receptor subtypes and act as partial agonists at 5-HT 2A . Conclusions In general, there were close parallels between the HTR data and the known SAR governing activity of phenylalkylamines at the 5-HT 2A receptor. These findings further support the classification of 2C-T compounds as psychedelic drugs.

Tree Foliage is a Methane Sink in Upland Temperate Forests - Adam S. Gorgolewski; John P. Caspersen; Juliana Vantellingen; Sean C. Thomas (Ecosystems, 2023)

Integrating knowledge on green infrastructure, health and well-being in ageing populations: Principles for research and practice - Matthew Dennis; Adam Barker; Jamie Anderson; Jenna C. Ashton; Gina Cavan; Penny A. Cook; David French; Anna Gilchrist; Philip James; Christopher Phillipson; Konstantinos Tzoulas; C. Philip Wheater; et al. (Ambio, 2023)
Ageing and urbanisation pose significant challenges for public health and urban planning. Ageing populations are at particular risk from hazards arising from urbanisation processes, some of which are in turn exacerbated by climate change. One approach for mitigating the negative effects of urbanisation on ageing populations is the leveraging of the beneficial effects of urban green infrastructure as a public health intervention in the planning process. We assessed the potential of available theoretical frameworks to provide the context for such leverage. This involved active engagement with academics and practitioners specialising in ageing, green infrastructure and health and well-being through a knowledge-brokering approach. We concluded that an integrated and comprehensive framework on the socio-cultural-ecological determinants of health is lacking. To address this, we present a set of principles for overcoming challenges to knowledge integration when working at the intersection of green infrastructure, ageing, health and well-being. Our findings—and the co-production process used to generate them—have wider significance for trans-disciplinary research into the benefits of the natural environment to human health and well-being as well as other complex and interconnected topics associated with global grand challenges.

Quantifying the effects of tides, river flow, and barriers on movements of Chinook Salmon smolts at junctions in the Sacramento – San Joaquin River Delta using multistate models - Michael J. Dodrill; Russell W. Perry; Adam C. Pope; Xiaochun Wang (Environmental Biology of Fishes, 2022)

2022 taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales - Jens H. Kuhn; Scott Adkins; Sergey V. Alkhovsky; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Martina Bandte; Martin Beer; Nicolas Bejerman; Éric Bergeron; et al. (Archives of Virology, 2022)

Forest biomes of Southern Africa - Ladislav Mucina; Mervyn C. Lötter; Michael C. Rutherford; Adriaan van Niekerk; Paul D. Macintyre; James L. Tsakalos; Jonathan Timberlake; Janine B. Adams; Taryn Riddin; Lauren K. Mccarthy (New Zealand Journal of Botany, 2022)
The forests of South Africa and the neighbouring countries, including Lesotho, eSwatini, Namibia, Botswana, Zimbabwe, and Mozambique (south of the Zambezi River), were mapped and classified according to the global system of biomes. The new four‐tier hierarchical biome system suggested in this paper includes zonobiome, global biome, continental biome (all recognised earlier), and regional biome – a novel biome category. The existing spatial coverages of the forests were revised and considerably improved, both in terms of forest‐patch coverage and mapping precision. Southern Africa is home to three zonal forest types, namely Subtropical Forests (Zonobiome I), Tropical Dry Forest (TDF; Zonobiome II) and Afrotemperate Forests (Zonobiome X). These three biomes are characterised by unique bioclimatic envelopes. Five, two, and eight regional biomes, respectively, have been recognised within these zonal biomes. Recognition of the Zonobiome I and the global biome Tropical Dry Forests in southern Africa is novel and expands our knowledge of the biome structure of African biotic communities. The system of the azonal regional biomes is also new and comprehensively covers the variability of the azonal helobiomes (riparian woodlands and swamp forests), mangroves, and azonal coastal forests. In total, 11 azonal regional biomes have been recognised in the study area. The forest biomes in southern Africa were captured in our electronic map in the form of more than 60 000 polygons, covering 42 416 km 2 (1.27% of the study area). No less than 83% of these forests occur in the territory of southern Mozambique.

Genetic structure and historic demography of endangered unarmoured threespine stickleback at southern latitudes signals a potential new management approach - Rachel Turba; Jonathan Q. Richmond; Sorel Fitz‐Gibbon; Marco Morselli; Robert N. Fisher; Camm C. Swift; Gorgonio Ruiz‐Campos; Adam R. Backlin; Chris Dellith; David K. Jacobs (Molecular Ecology, 2022)
Habitat loss, flood control infrastructure, and drought have left most of southern California and northern Baja California's native freshwater fish near extinction, including the endangered unarmoured threespine stickleback ( Gasterosteus aculeatus williamsoni ). This subspecies, an unusual morph lacking the typical lateral bony plates of the G. aculeatus complex, occurs at arid southern latitudes in the eastern Pacific Ocean and survives in only three inland locations. Managers have lacked molecular data to answer basic questions about the ancestry and genetic distinctiveness of unarmoured populations. These data could be used to prioritize conservation efforts. We sampled G. aculeatus from 36 localities and used microsatellites and whole genome data to place unarmoured populations within the broader evolutionary context of G. aculeatus across southern California/northern Baja California. We identified three genetic groups with none consisting solely of unarmoured populations. Unlike G. aculeatus at northern latitudes, where Pleistocene glaciation has produced similar historical demographic profiles across populations, we found markedly different demographics depending on sampling location, with inland unarmoured populations showing steeper population declines and lower heterozygosity compared to low armoured populations in coastal lagoons. One exception involved the only high elevation population in the region, where the demography and alleles of unarmoured fish were similar to low armoured populations near the coast, exposing one of several cases of artificial translocation. Our results suggest that the current “management‐by‐phenotype” approach, based on lateral plates, is incidentally protecting the most imperilled populations; however, redirecting efforts toward evolutionary units, regardless of phenotype, may more effectively preserve adaptive potential.

Linking changes in species composition and biomass in a globally distributed grassland experiment - Emma Ladouceur; Shane A. Blowes; Jonathan M. Chase; Adam T. Clark; Magda Garbowski; Juan Alberti; Carlos Alberto Arnillas; Jonathan D. Bakker; Isabel C. Barrio; Siddharth Bharath; Elizabeth T. Borer; Lars A. Brudvig; et al. (Ecology Letters, 2022)
Global change drivers, such as anthropogenic nutrient inputs, are increasing globally. Nutrient deposition simultaneously alters plant biodiversity, species composition and ecosystem processes like aboveground biomass production. These changes are underpinned by species extinction, colonisation and shifting relative abundance. Here, we use the Price equation to quantify and link the contributions of species that are lost, gained or that persist to change in aboveground biomass in 59 experimental grassland sites. Under ambient (control) conditions, compositional and biomass turnover was high, and losses (i.e. local extinctions) were balanced by gains (i.e. colonisation). Under fertilisation, the decline in species richness resulted from increased species loss and decreases in species gained. Biomass increase under fertilisation resulted mostly from species that persist and to a lesser extent from species gained. Drivers of ecological change can interact relatively independently with diversity, composition and ecosystem processes and functions such as aboveground biomass due to the individual contributions of species lost, gained or persisting.

Priority effects in coral–macroalgae interactions can drive alternate community paths in the absence of top‐down control - Thomas C. Adam; Sally J. Holbrook; Deron E. Burkepile; Kelly E. Speare; Andrew J. Brooks; Mark C. Ladd; Andrew A. Shantz; Rebecca Vega Thurber; Russell J. Schmitt (Ecology, 2022)
The outcomes of species interactions can vary greatly in time and space with the outcomes of some interactions determined by priority effects. On coral reefs, benthic algae rapidly colonize disturbed substrate. In the absence of top‐down control from herbivorous fishes, these algae can inhibit the recruitment of reef‐building corals, leading to a persistent phase shift to a macroalgae‐dominated state. Yet, corals may also inhibit colonization by macroalgae, and therefore the effects of herbivores on algal communities may be strongest following disturbances that reduce coral cover. Here, we report the results from experiments conducted on the fore reef of Moorea, French Polynesia, where we: (1) tested the ability of macroalgae to invade coral‐dominated and coral‐depauperate communities under different levels of herbivory, (2) explored the ability of juvenile corals ( Pocillopora spp.) to suppress macroalgae, and (3) quantified the direct and indirect effects of fish herbivores and corallivores on juvenile corals. We found that macroalgae proliferated when herbivory was low but only in recently disturbed communities where coral cover was also low. When coral cover was

Benthic species patterns in and around the Cape Canyon: A large submarine canyon off the western passive margin of South Africa - Zoleka Filander; Adam N. H. Smith; Hayley C. Cawthra; Tarron Lamont (Frontiers in Marine Science, 2022)
Although submarine canyons are internationally recognized as sensitive ecosystems and reported to be biological hotspots, regional studies are required to validate this consensus. To this end, hydrographic and benthic biodiversity data were collected during three cruises (2016-2017) to provide insights on the benthic patterns within South African canyon and non-canyon offshore areas. A total of 25 stations, sampled at 200-1000 m depth range, form the basis of the multivariate analysis. Diversity gradients were calculated and then differences were compared across substrate types and depth zones represented within 12 canyon and 13 non-canyon stations. Significant differences in both substrate and depth were evident, despite measures being highly variable. This observation of varying diversity in different substrates is in line with previous studies. No clear pattern was observed for species diversity (delta+). However, non-canyon stations overall showed a higher diversity in comparison to canyon stations. A notable peak in diversity is observed in canyon areas in the 401-500 m depth zone. Species richness followed an opposing pattern, as it decreased with depth and was consistently higher in canyon areas. These results align with the well-defined influence of depth-related variables on the distribution of taxonomic groups and the substrate available, at various scales. The eutrophic characteristic of the Benguela region may have attributed to the insignificant diversity differences between canyon and non-canyon stations. To assess the benthic species structure in canyon and non-canyon areas, we converted the 108 benthic species into a gamma+ matrix. We then modelled the biological response to predictor variables (substrate and depth). Although the canyon and non-canyon areas have an overlapping species composition, the main effects (canyon vs. non-canyon, depth, and substrate) showed significant differences. Thirteen species were characteristic of canyon areas, whilst only three distinguished non-canyon areas. The region has a long history of anthropogenic activities, so the observed benthic profiles may already be altered. The current study therefore provides the first detailed taxonomic description and analysis of benthic species profiles in the Cape Canyon, and advances important baseline information necessary for understanding the ecological importance of the Cape Canyon.

Archean and Paleoproterozoic zircons in Paleozoic sandstones in southern New Zealand: evidence for remnant Nuna supercontinent and Ur continent rocks within Zealandia - C. J. Adams; W. R. H. Ramsay (Australian Journal of Earth Sciences, 2022)

Conceptualizing a probabilistic risk and loss assessment framework for wildfires - Negar Elhami-Khorasani; Hamed Ebrahimian; Lawrence Buja; Susan L. Cutter; Branko Kosovic; Neil Lareau; Brian J. Meacham; Eric Rowell; Ertugrul Taciroglu; Matthew P. Thompson; Adam C. Watts (Natural Hazards, 2022)

Concussion Prone Scenarios: A Multi-Dimensional Exploration in Impact Directions, Brain Morphology, and Network Architectures Using Computational Models - Taotao Wu; Jared A. Rifkin; Adam C. Rayfield; Erin D. Anderson; Matthew B. Panzer; David F. Meaney (Annals of Biomedical Engineering, 2022)

Head Impact Exposure and Biomechanics in University Varsity Women’s Soccer - Rebecca Kenny; Marko Elez; Adam Clansey; Naznin Virji-Babul; Lyndia C. Wu (Annals of Biomedical Engineering, 2022)

The missing link in biogeographic reconstruction: Accounting for lineage extinction rewrites history - Leonel Herrera‐Alsina; Adam C. Algar; Lesley T. Lancaster; Juan Francisco Ornelas; Greta Bocedi; Alexander S. T. Papadopulos; Cecile Gubry‐Rangin; Owen G. Osborne; Poppy Mynard; I. Made Sudiana; Pungki Lupiyaningdyah; Berry Juliandi; et al. (Journal of Biogeography, 2022)
Aim In the most widely used family of methods for ancestral range estimation (ARE), dispersal, speciation and extirpation events are estimated from information on extant lineages. However, this approach fails to consider the geographic distribution of extinct species and their position on the phylogenetic tree, an omission that could compromise reconstruction. Here, we present a method that models the geographic distribution of extinct species and we quantify the potential inaccuracy in ancestral range estimation when extinction rates are above zero. Location Global applications, with an example from the Americas. Taxon All taxa, with an example from hummingbirds ( Amazilia ). Methods Methods capable of explicitly modelling extinct branches along with their reconstructed geographic information (GeoSSE) have been overlooked in ARE analysis, perhaps due to the inherent complexity of implementation. We develop a user‐friendly platform, which we term LEMAD (Lineage Extinction Model of Ancestral Distribution) that generalizes the likelihood described in GeoSSE for any number of areas and under several sets of geographic assumptions. We compare LEMAD and extinction‐free approaches using extensive simulations under different macroevolutionary scenarios. We apply our method to revisit the historical biogeography of Amazilia hummingbirds. Results We find that accounting for the lineages removed from a tree by extinction improves reconstructions of ancestral distributions, especially when rates of vicariant speciation are higher than rates of in situ speciation, and when rates of extinction and range evolution are high. Rates of in situ and vicariant speciation are accurately estimated by LEMAD in all scenarios. North America as the most likely region for the common ancestor of hummingbirds. Main conclusions Methods that neglect lineage extinction are less likely to accurately reconstruct true biogeographic histories of extant clades. Our findings on an empirical dataset reconcile the Eurasian origin of Amazilia with biogeographic reconstructions when lineage extinction is considered.

Local groundwater decline exacerbates response of dryland riparian woodlands to climatic drought - Jared Williams; John C. Stella; Steven L. Voelker; Adam M. Lambert; Lissa M. Pelletier; John E. Drake; Jonathan M. Friedman; Dar A. Roberts; Michael Bliss Singer (Global Change Biology, 2022)
Dryland riparian woodlands are considered to be locally buffered from droughts by shallow and stable groundwater levels. However, climate change is causing more frequent and severe drought events, accompanied by warmer temperatures, collectively threatening the persistence of these groundwater dependent ecosystems through a combination of increasing evaporative demand and decreasing groundwater supply. We conducted a dendro‐isotopic analysis of radial growth and seasonal (semi‐annual) carbon isotope discrimination (Δ 13 C) to investigate the response of riparian cottonwood stands to the unprecedented California‐wide drought from 2012 to 2019, along the largest remaining free‐flowing river in Southern California. Our goals were to identify principal drivers and indicators of drought stress for dryland riparian woodlands, determine their thresholds of tolerance to hydroclimatic stressors, and ultimately assess their vulnerability to climate change. Riparian trees were highly responsive to drought conditions along the river, exhibiting suppressed growth and strong stomatal closure (inferred from reduced Δ 13 C) during peak drought years. However, patterns of radial growth and Δ 13 C were quite variable among sites that differed in climatic conditions and rate of groundwater decline. We show that the rate of groundwater decline, as opposed to climate factors, was the primary driver of site differences in drought stress, and trees showed greater sensitivity to temperature at sites subjected to faster groundwater decline. Across sites, higher correlation between radial growth and Δ 13 C for individual trees, and higher inter‐correlation of Δ 13 C among trees were indicative of greater drought stress. Trees showed a threshold of tolerance to groundwater decline at 0.5 m year −1 beyond which drought stress became increasingly evident and severe. For sites that exceeded this threshold, peak physiological stress occurred when total groundwater recession exceeded ~3 m. These findings indicate that drought‐induced groundwater decline associated with more extreme droughts is a primary threat to dryland riparian woodlands and increases their susceptibility to projected warmer temperatures.

Ecophysiological and genomic analyses of a representative isolate of highly abundant Bacillus cereus strains in contaminated subsurface sediments - Jennifer L. Goff; Elizabeth G. Szink; Michael P. Thorgersen; Andrew D. Putt; Yupeng Fan; Lauren M. Lui; Torben N. Nielsen; Kristopher A. Hunt; Jonathan P. Michael; Yajiao Wang; Daliang Ning; Ying Fu; et al. (Environmental Microbiology, 2022)
Bacillus cereus strain CPT56D‐587‐MTF (CPTF) was isolated from the highly contaminated Oak Ridge Reservation (ORR) subsurface. This site is contaminated with high levels of nitric acid and multiple heavy metals. Amplicon sequencing of the 16S rRNA genes (V4 region) in sediment from this area revealed an amplicon sequence variant (ASV) with 100% identity to the CPTF 16S rRNA sequence. Notably, this CPTF‐matching ASV had the highest relative abundance in this community survey, with a median relative abundance of 3.77% and comprised 20%–40% of reads in some samples. Pangenomic analysis revealed that strain CPTF has expanded genomic content compared to other B. cereus species—largely due to plasmid acquisition and expansion of transposable elements. This suggests that these features are important for rapid adaptation to native environmental stressors. We connected genotype to phenotype in the context of the unique geochemistry of the site. These analyses revealed that certain genes (e.g. nitrate reductase, heavy metal efflux pumps) that allow this strain to successfully occupy the geochemically heterogenous microniches of its native site are characteristic of the B. cereus species while others such as acid tolerance are mobile genetic element associated and are generally unique to strain CPTF.

Comparisons between sequenced and re-sequenced genomes of historical subterranean clover mottle virus isolates - John Fosu-Nyarko; Ian P. Adams; Michael G. K. Jones; Adrian Fox; Roger A. C. Jones (Journal of Plant Pathology, 2022)
We report comparisons between the complete genomic sequences of five historical Western Australian isolates of subterranean clover mottle virus (SCMoV) from 1989–2000, and an infectious clone of its 1989 isolate. Sanger Sequencing (SS) and High Throughput Sequencing (HTS), or both, were used to obtain these genomes. Four of the SCMoV isolates were sequenced by SS in 1999–2002, but re-sequenced again by HTS in 2020. The pairs of sequences obtained from these four isolates differed by only 18–59 nucleotides. This small difference resulted from the different sequencing methods, the < 1–5 years each isolate was host passaged before freeze-drying prior to HTS sequencing, or a combination of both. Since SCMoV has not been reported outside Australia, this similarity suggests the population sequenced represents the progeny of either an indigenous virus that spread from a native legume to subterranean clover after its introduction or a recent seed-borne incursion from elsewhere. The ORF1 was the most variable, and the phylogenetic tree constructed with ORF1s showed the isolates grouped according to their symptom severity in subterranean clover, indicating the probability that ORF1-encoded P1 protein is a symptom determinant. A satellite RNA was associated with all SCMoV genomes obtained by HTS but none derived by SS.

Detrital zircons in Triassic–Cretaceous sandstones, Clarence-Moreton Basin, eastern Australia: speculations upon Australia and Zealandia provenances - C. J. Adams; H. J. Campbell; R. J. Korsch; W. L. Griffin (Australian Journal of Earth Sciences, 2022)

Complement C3a Receptor (C3aR) Mediates Vascular Dysfunction, Hippocampal Pathology, and Cognitive Impairment in a Mouse Model of VCID - Kanchan Bhatia; Adam Kindelin; Muhammad Nadeem; Mohammad Badruzzaman Khan; Junxiang Yin; Alberto Fuentes; Karis Miller; Gregory H. Turner; Mark C. Preul; Abdullah S. Ahmad; Elliott J. Mufson; Michael F. Waters; et al. (Translational Stroke Research, 2022)

Using Biological Responses to Monitor Freshwater Post-Spill Conditions over 3 years in Blacktail Creek, North Dakota, USA - Aїda M. Farag; David D. Harper; Isabelle M. Cozzarelli; Douglas B. Kent; Adam C. Mumford; Denise M. Akob; Travis Schaeffer; Luke R. Iwanowicz (Archives of Environmental Contamination and Toxicology, 2022)

Characterizing spatial and temporal variation in stable hydrogen isotopes (δ2H) between two distinct lentic freshwater food webs - Michael C. Cavallaro; Chantel I. Michelson; Tyler L. Lewis; Adam DuBour; Mark Lindberg; Keith A. Hobson; Robert G. Clark (Aquatic Sciences, 2022)

SNAPSHOT USA 2020: A second coordinated national camera trap survey of the United States during the COVID ‐19 pandemic - Roland Kays; Michael V. Cove; Jose Diaz; Kimberly Todd; Claire Bresnan; Matt Snider; Thomas E. Lee; Jonathan G. Jasper; Brianna Douglas; Anthony P. Crupi; Katherine C. B. Weiss; Helen Rowe; et al. (Ecology, 2022)
Managing wildlife populations in the face of global change requires regular data on the abundance and distribution of wild animals, but acquiring these over appropriate spatial scales in a sustainable way has proven challenging. Here we present the data from Snapshot USA 2020, a second annual national mammal survey of the USA. This project involved 152 scientists setting camera traps in a standardized protocol at 1485 locations across 103 arrays in 43 states for a total of 52,710 trap‐nights of survey effort. Most (58) of these arrays were also sampled during the same months (September and October) in 2019, providing a direct comparison of animal populations in 2 years that includes data from both during and before the COVID‐19 pandemic. All data were managed by the eMammal system, with all species identifications checked by at least two reviewers. In total, we recorded 117,415 detections of 78 species of wild mammals, 9236 detections of at least 43 species of birds, 15,851 detections of six domestic animals and 23,825 detections of humans or their vehicles. Spatial differences across arrays explained more variation in the relative abundance than temporal variation across years for all 38 species modeled, although there are examples of significant site‐level differences among years for many species. Temporal results show how species allocate their time and can be used to study species interactions, including between humans and wildlife. These data provide a snapshot of the mammal community of the USA for 2020 and will be useful for exploring the drivers of spatial and temporal changes in relative abundance and distribution, and the impacts of species interactions on daily activity patterns. There are no copyright restrictions, and please cite this paper when using these data, or a subset of these data, for publication.

Eruptive history of Mason Spur, a Miocene—Pleistocene polygenetic volcanic complex in southern Victoria Land, West Antarctic Rift System, Antarctica - John L. Smellie; Adam P. Martin; Gianfranco Di Vincenzo; Dougal B. Townsend; Matthew T. Heizler; Dawn C. S. Ruth (Bulletin of Volcanology, 2022)
Mason Spur is a deeply eroded Middle Miocene to Pleistocene (c. 13 to 0.37 Ma) volcanic complex in southern Victoria Land, within the West Antarctic Rift System (WARS). The oldest rocks include a large volume of trachyte ignimbrites that provided abundant volcanic detritus recovered in McMurdo Sound drill cores. The ignimbrites together with early-formed intrusions were strongly deformed during a substantial caldera collapse at c. 13 Ma. Intense erosion modified the volcanic landscape, creating a paleo-relief of several hundred metres. Deep ravines were cut and filled by deposits of multiple lahars probably linked to gravitational collapses of trachyte dome(s). Small-volume trachytic magmas were also erupted, forming lavas and at least one tuff cone. The youngest trachytic activity comprises a lava dome and related block-and-ash-flow deposits, erupted at 6 Ma. Basanite erupted throughout the history of the complex and eruptions younger than 12 Ma are almost exclusively basanite, forming scoria cones, water-cooled lavas, and tuff cones. Three peripheral outcrops are composed of basanitic ‘a‘ā lava-fed deltas, probably erupted from vents on neighbouring volcanoes at Mount Discovery and Mount Morning. Abundant ignimbrite deposits at Mason Spur differentiate this volcanic complex from others in the WARS. Eruptions were triggered by rift extension initially, yielding the voluminous trachytes sourced from a magma chamber on the margin of the WARS. Later mafic eruptions were associated with deep crustal faults related to residual intraplate deformation. These results add important details to the eruptive history of the intracontinental WARS.

Correction to: The Impact of Right Temporal Lobe Epilepsy on Nonverbal Memory: Meta-regression of Stimulus- and Task-related Moderators - Adam C. Bentvelzen; Roy P. C. Kessels; Nicholas A. Badcock; Greg Savage (Neuropsychology Review, 2022)

The Impact of Right Temporal Lobe Epilepsy On Nonverbal Memory: Meta-regression of Stimulus- and Task-related Moderators - Adam C. Bentvelzen; Roy P. C. Kessels; Nicholas A. Badcock; Greg Savage (Neuropsychology Review, 2022)

Forty-year-old beet ringspot virus isolate: tests for potato true seed transmission, genome sequencing, recombination analysis and phylogenetic placement - Roger A. C. Jones; Aimee R. Fowkes; Sam McGreig; Adrian Fox; Ian P. Adams (Journal of Plant Pathology, 2022)
Summary Beet ringspot virus (BRSV) isolate IF came from a 1980 bait test on a Scottish soil sample. Between 1982 and 1984, it was used to study possible BRSV seed transmission in potato ( Solanum tuberosum ). To encourage flowering, potato scions were graft-inoculated onto tomato plants and these grafted plants were inoculated with IF. Flowers on BRSV-infected potato shoots were self-pollinated and their seeds collected. BRSV was found readily in pollen from these potato flowers, but never detected in any seedlings that grew from their seeds. Thus, although BRSV was present in pollen, no evidence of potato true seed transmission was obtained. In 1985, IF was preserved by freeze-drying and kept thereafter in a historical virus isolate collection. In 2020, it was subjected to high throughput sequencing and a complete genomic sequence was obtained. No recombination was detected in the IF sequence but its RNA1 and RNA2 were major parents of three, and a minor parent of one, recombinant BRSV isolates, respectively. Phylogenetic analysis of the P1 and P2 components of the coat protein gene encoded on its RNA2 placed the isolate IF sequence within the BRSV lineage. However, too few other BRSV sequences from different countries were present to provide information on its geographical associations.

Quantifying baseline costs and cataloging potential optimization strategies for kelp aquaculture carbon dioxide removal - Struan Coleman; Tobias Dewhurst; David W. Fredriksson; Adam T. St. Gelais; Kelly L. Cole; Michael MacNicoll; Eric Laufer; Damian C. Brady (Frontiers in Marine Science, 2022)
To keep global surface warming below 1.5°C by 2100, the portfolio of cost-effective CDR technologies must expand. To evaluate the potential of macroalgae CDR, we developed a kelp aquaculture bio-techno-economic model in which large quantities of kelp would be farmed at an offshore site, transported to a deep water “sink site”, and then deposited below the sequestration horizon (1,000 m). We estimated the costs and associated emissions of nursery production, permitting, farm construction, ocean cultivation, biomass transport, and Monitoring, Reporting, and Verification (MRV) for a 1,000 acre (405 ha) “baseline” project located in the Gulf of Maine, USA. The baseline kelp CDR model applies current systems of kelp cultivation to deep water (100 m) exposed sites using best available modeling methods. We calculated the levelized unit costs of CO 2 eq sequestration (LCOC; $ tCO 2 eq -1 ). Under baseline assumptions, LCOC was $17,048 tCO 2 eq -1 . Despite annually sequestering 628 tCO 2 eq within kelp biomass at the sink site, the project was only able to net 244 C credits (tCO 2 eq) each year, a true sequestration “additionality” rate (AR) of 39% (i.e., the ratio of net C credits produced to gross C sequestered within kelp biomass). As a result of optimizing 18 key parameters for which we identified a range within the literature, LCOC fell to $1,257 tCO 2 eq -1 and AR increased to 91%, demonstrating that substantial cost reductions could be achieved through process improvement and decarbonization of production supply chains. Kelp CDR may be limited by high production costs and energy intensive operations, as well as MRV uncertainty. To resolve these challenges, R&D must (1) de-risk farm designs that maximize lease space, (2) automate the seeding and harvest processes, (3) leverage selective breeding to increase yields, (4) assess the cost-benefit of gametophyte nursery culture as both a platform for selective breeding and driver of operating cost reductions, (5) decarbonize equipment supply chains, energy usage, and ocean cultivation by sourcing electricity from renewables and employing low GHG impact materials with long lifespans, and (6) develop low-cost and accurate MRV techniques for ocean-based CDR.

Glucose metabolism patterns: A potential index to characterize brain ageing and predict high conversion risk into cognitive impairment - Jiehui Jiang; Can Sheng; Guanqun Chen; Chunhua Liu; Shichen Jin; Lanlan Li; Xueyan Jiang; Ying Han; Michael W. Weiner; Paul Aisen; Ronald Petersen; Clifford R. Jack; et al. (GeroScience, 2022)

Correction to: Evolutionary allometry of sexual dimorphism of jumping performance in anurans - Bryan H. Juarez; Dean C. Adams (Evolutionary Ecology, 2022)

Evolutionary allometry of sexual dimorphism of jumping performance in anurans - Bryan H. Juarez; Dean C. Adams (Evolutionary Ecology, 2022)

Portable X-ray fluorescence (pXRF) analysis of heavy metal contamination in church graveyards with contrasting soil types - Charles Madden; Jamie K. Pringle; Adam J. Jeffery; Kristopher D. Wisniewski; Vivienne Heaton; Ian W. Oliver; Helen Glanville; Ian G. Stimpson; Henry C. Dick; Madeleine Eeley; Jonathan Goodwin (Environmental Science and Pollution Research, 2022)
Human remains have been interred in burial grounds since historic times. Although the re-use of graveyards differs from one country, region or time period to another, over time, graveyard soil may become contaminated or enriched with heavy metal elements. This paper presents heavy metal element soil analysis from two UK church graveyard study sites with contrasting necrosols, but similar burial densities and known burial ages dating back to the sixteenth century and some possibly older than 1,000 years. Portable X-ray fluorescence element laboratory-based analyses were undertaken on surface and near-surface soil pellets. Results show elevated levels of Fe, Pb, Mn, Cr, Cu, Zn and Ca in both necrosols when compared with background values. Element concentration anomalies remained consistently higher than background samples down to 2 m, but reduced with distance away from church buildings. Element concentration anomalies are higher in the clay-rich necrosol than in sandy necrosol. Study result implications suggest that long-used necrosols are likely to be more contaminated with heavy metal elements than similar soil outside graveyards with implications for burial grounds management, adjacent populations and where burial grounds have been deconsecrated and turned to residential dwellings.

Giant Tree Frog diversification in West and Central Africa: Isolation by physical barriers, climate, and reproductive traits - Kyle E. Jaynes; Edward A. Myers; Václav Gvoždík; David C. Blackburn; Daniel M. Portik; Eli Greenbaum; Gregory F. M. Jongsma; Mark‐Oliver Rödel; Gabriel Badjedjea; Abraham Bamba‐Kaya; Ninda L. Baptista; Jeannot B. Akuboy; et al. (Molecular Ecology, 2022)
Secondary sympatry amongst sister lineages is strongly associated with genetic and ecological divergence. This pattern suggests that for closely related species to coexist in secondary sympatry, they must accumulate differences in traits that mediate ecological and/or reproductive isolation. Here, we characterized inter‐ and intraspecific divergence in three giant tree frog species whose distributions stretch across West and Central Africa. Using genome‐wide single‐nucleotide polymorphism data, we demonstrated that species‐level divergence coincides temporally and geographically with a period of large‐scale forest fragmentation during the late Pliocene. Our environmental niche models further supported a dynamic history of climatic suitability and stability, and indicated that all three species occupy distinct environmental niches. We found modest morphological differentiation amongst the species with significant divergence in tympanum diameter and male advertisement call. In addition, we confirmed that two species occur in secondary sympatry in Central Africa but found no evidence of hybridization. These patterns support the hypothesis that cycles of genetic exchange and isolation across West and Central Africa have contributed to globally significant biodiversity. Furthermore, divergence in both ecology and reproductive traits appear to have played important roles in maintaining distinct lineages. At the intraspecific level, we found that climatic refugia, precipitation gradients, marine incursions, and potentially riverine barriers generated phylogeographic structure throughout the Pleistocene and into the Holocene. Further studies examining phenotypic divergence and secondary contact amongst these geographically structured populations may demonstrate how smaller scale and more recent biogeographic barriers contribute to regional diversification.

Evaluating expert‐based habitat suitability information of terrestrial mammals with GPS‐ tracking data - Maarten J. E. Broekman; Jelle P. Hilbers; Mark A. J. Huijbregts; Thomas Mueller; Abdullahi H. Ali; Henrik Andrén; Jeanne Altmann; Malin Aronsson; Nina Attias; Hattie L. A. Bartlam‐Brooks; Floris M. van Beest; Jerrold L. Belant; et al. (Global Ecology and Biogeography, 2022)
Aim Macroecological studies that require habitat suitability data for many species often derive this information from expert opinion. However, expert‐based information is inherently subjective and thus prone to errors. The increasing availability of GPS tracking data offers opportunities to evaluate and supplement expert‐based information with detailed empirical evidence. Here, we compared expert‐based habitat suitability information from the International Union for Conservation of Nature (IUCN) with habitat suitability information derived from GPS‐tracking data of 1,498 individuals from 49 mammal species. Location Worldwide. Time period 1998–2021. Major taxa studied Forty‐nine terrestrial mammal species. Methods Using GPS data, we estimated two measures of habitat suitability for each individual animal: proportional habitat use (proportion of GPS locations within a habitat type), and selection ratio (habitat use relative to its availability). For each individual we then evaluated whether the GPS‐based habitat suitability measures were in agreement with the IUCN data. To that end, we calculated the probability that the ranking of empirical habitat suitability measures was in agreement with IUCN's classification into suitable, marginal and unsuitable habitat types. Results IUCN habitat suitability data were in accordance with the GPS data (> 95% probability of agreement) for 33 out of 49 species based on proportional habitat use estimates and for 25 out of 49 species based on selection ratios. In addition, 37 and 34 species had a > 50% probability of agreement based on proportional habitat use and selection ratios, respectively. Main conclusions We show how GPS‐tracking data can be used to evaluate IUCN habitat suitability data. Our findings indicate that for the majority of species included in this study, it is appropriate to use IUCN habitat suitability data in macroecological studies. Furthermore, we show that GPS‐tracking data can be used to identify and prioritize species and habitat types for re‐evaluation of IUCN habitat suitability data.

Identification of genetic loci in lettuce mediating quantitative resistance to fungal pathogens - Harry Pink; Adam Talbot; Abi Graceson; Juliane Graham; Gill Higgins; Andrew Taylor; Alison C. Jackson; Maria Truco; Richard Michelmore; Chenyi Yao; Frances Gawthrop; David Pink; et al. (Theoretical and Applied Genetics, 2022)
Key message We demonstrate genetic variation for quantitative resistance against important fungal pathogens in lettuce and its wild relatives, map loci conferring resistance and predict key molecular mechanisms using transcriptome profiling. Abstract Lactuca sativa L. (lettuce) is an important leafy vegetable crop grown and consumed globally. Chemicals are routinely used to control major pathogens, including the causal agents of grey mould ( Botrytis cinerea ) and lettuce drop ( Sclerotinia sclerotiorum ) . With increasing prevalence of pathogen resistance to fungicides and environmental concerns, there is an urgent need to identify sources of genetic resistance to B. cinerea and S. sclerotiorum in lettuce. We demonstrated genetic variation for quantitative resistance to B. cinerea and S. sclerotiorum in a set of 97 diverse lettuce and wild relative accessions, and between the parents of lettuce mapping populations. Transcriptome profiling across multiple lettuce accessions enabled us to identify genes with expression correlated with resistance, predicting the importance of post-transcriptional gene regulation in the lettuce defence response. We identified five genetic loci influencing quantitative resistance in a F 6 mapping population derived from a Lactuca serriola (wild relative) × lettuce cross, which each explained 5–10% of the variation. Differential gene expression analysis between the parent lines, and integration of data on correlation of gene expression and resistance in the diversity set, highlighted potential causal genes underlying the quantitative trait loci.

Climatic and biogeographical drivers of functional diversity in the flora of the Canary Islands - Dagmar M. Hanz; Vanessa Cutts; Martha Paola Barajas‐Barbosa; Adam C. Algar; Carl Beierkuhnlein; José‐María Fernández‐Palacios; Richard Field; Holger Kreft; Manuel J. Steinbauer; Patrick Weigelt; Severin D. H. Irl (Global Ecology and Biogeography, 2022)
Aim Functional traits can help us to elucidate biogeographical and ecological processes driving assemblage structure. We analysed the functional diversity of plant species of different evolutionary origins across an island archipelago, along environmental gradients and across geological age, to assess functional aspects of island biogeographical theory. Location Canary Islands, Spain. Major taxa studied Spermatophytes. Time period Present day. Methods We collected data for four traits (plant height, leaf length, flower length and fruit length) associated with resource acquisition, competitive ability, reproduction and dispersal ability of 893 endemic, non‐endemic native and alien plant species ( c . 43% of the Canary Island flora) from the literature. Linking these traits to species occurrences and composition across a 500 m × 500 m grid, we calculated functional diversity for endemic, non‐endemic native and alien assemblages using multidimensional functional hypervolumes and related the resulting patterns to climatic (humidity) and island biogeographical (geographical isolation, topographic complexity and geological age) gradients. Results Trait space of endemic and non‐endemic native species overlapped considerably, and alien species added novel trait combinations, expanding the overall functional space of the Canary Islands. We found that functional diversity of endemic plant assemblages was highest in geographically isolated and humid grid cells. Functional diversity of non‐endemic native assemblages was highest in less isolated and humid grid cells. In contrast, functional diversity of alien assemblages was highest in arid ecosystems. Topographic complexity and geological age had only a subordinate effect on functional diversity across floristic groups. Main conclusions We found that endemic and non‐endemic native island species possess similar traits, whereas alien species tend to expand functional space in ecosystems where they have been introduced. The spatial distribution of the functional diversity of floristic groups is very distinct across environmental gradients, indicating that species assemblages of different evolutionary origins thrive functionally in dissimilar habitats.

Interactive Effects of HIV Infection and Cannabis Use on Insula Subregion Functional Connectivity - Jessica S. Flannery; Michael C. Riedel; Taylor Salo; Lauren D. Hill-Bowen; Ranjita Poudel; Ashley R. Adams; Angela R. Laird; Raul Gonzalez; Matthew T. Sutherland (Journal of Neuroimmune Pharmacology, 2022)

Morphological and Functional Divergence of the Lower Jaw Between Native and Invasive Red Foxes - Colline Brassard; Jesse L. Forbes-Harper; Heather M. Crawford; John-Michael Stuart; Natalie M. Warburton; Michael C. Calver; Peter Adams; Elodie Monchâtre-Leroy; Jacques Barrat; Sandrine Lesellier; Claude Guintard; Hélène Garès; et al. (Journal of Mammalian Evolution, 2022)

Comprehensive analysis of epigenetic clocks reveals associations between disproportionate biological ageing and hippocampal volume - Lidija Milicic; Michael Vacher; Tenielle Porter; Vincent Doré; Samantha C. Burnham; Pierrick Bourgeat; Rosita Shishegar; James Doecke; Nicola J. Armstrong; Rick Tankard; Paul Maruff; Colin L. Masters; et al. (GeroScience, 2022)
The concept of age acceleration, the difference between biological age and chronological age, is of growing interest, particularly with respect to age-related disorders, such as Alzheimer’s Disease (AD). Whilst studies have reported associations with AD risk and related phenotypes, there remains a lack of consensus on these associations. Here we aimed to comprehensively investigate the relationship between five recognised measures of age acceleration, based on DNA methylation patterns (DNAm age), and cross-sectional and longitudinal cognition and AD-related neuroimaging phenotypes (volumetric MRI and Amyloid-β PET) in the Australian Imaging, Biomarkers and Lifestyle (AIBL) and the Alzheimer’s Disease Neuroimaging Initiative (ADNI). Significant associations were observed between age acceleration using the Hannum epigenetic clock and cross-sectional hippocampal volume in AIBL and replicated in ADNI. In AIBL, several other findings were observed cross-sectionally, including a significant association between hippocampal volume and the Hannum and Phenoage epigenetic clocks. Further, significant associations were also observed between hippocampal volume and the Zhang and Phenoage epigenetic clocks within Amyloid-β positive individuals. However, these were not validated within the ADNI cohort. No associations between age acceleration and other Alzheimer’s disease-related phenotypes, including measures of cognition or brain Amyloid-β burden, were observed, and there was no association with longitudinal change in any phenotype. This study presents a link between age acceleration, as determined using DNA methylation, and hippocampal volume that was statistically significant across two highly characterised cohorts. The results presented in this study contribute to a growing literature that supports the role of epigenetic modifications in ageing and AD-related phenotypes.

Prediction of disability-free survival in healthy older people - Johannes Tobias Neumann; Le T. P. Thao; Anne M. Murray; Emily Callander; Prudence R. Carr; Mark R. Nelson; Rory Wolfe; Robyn L. Woods; Christopher M. Reid; Raj C. Shah; Anne B. Newman; Jeff D. Williamson; et al. (GeroScience, 2022)
Prolonging survival in good health is a fundamental societal goal. However, the leading determinants of disability-free survival in healthy older people have not been well established. Data from ASPREE, a bi-national placebo-controlled trial of aspirin with 4.7 years median follow-up, was analysed. At enrolment, participants were healthy and without prior cardiovascular events, dementia or persistent physical disability. Disability-free survival outcome was defined as absence of dementia, persistent disability or death. Selection of potential predictors from amongst 25 biomedical, psychosocial and lifestyle variables including recognized geriatric risk factors, utilizing a machine-learning approach. Separate models were developed for men and women. The selected predictors were evaluated in a multivariable Cox proportional hazards model and validated internally by bootstrapping. We included 19,114 Australian and US participants aged ≥65 years (median 74 years, IQR 71.6–77.7). Common predictors of a worse prognosis in both sexes included higher age, lower Modified Mini-Mental State Examination score, lower gait speed, lower grip strength and abnormal (low or elevated) body mass index. Additional risk factors for men included current smoking, and abnormal eGFR. In women, diabetes and depression were additional predictors. The biased-corrected areas under the receiver operating characteristic curves for the final prognostic models at 5 years were 0.72 for men and 0.75 for women. Final models showed good calibration between the observed and predicted risks. We developed a prediction model in which age, cognitive function and gait speed were the strongest predictors of disability-free survival in healthy older people. Trial registration Clinicaltrials.gov (NCT01038583)

Higher white matter hyperintensity load adversely affects pre-post proximal cognitive training performance in healthy older adults - Emanuel M. Boutzoukas; Andrew O’Shea; Jessica N. Kraft; Cheshire Hardcastle; Nicole D. Evangelista; Hanna K. Hausman; Alejandro Albizu; Emily J. Van Etten; Pradyumna K. Bharadwaj; Samantha G. Smith; Hyun Song; Eric C. Porges; et al. (GeroScience, 2022)

Clinical and molecular evaluation of MEFV gene variants in the Turkish population: a study by the National Genetics Consortium - Munis Dundar; Umut Fahrioglu; Saliha Handan Yildiz; Burcu Bakir-Gungor; Sehime Gulsun Temel; Haluk Akin; Sevilhan Artan; Tulin Cora; Feride Iffet Sahin; Ahmet Dursun; Ozlem Sezer; Hakan Gurkan; et al. (Functional & Integrative Genomics, 2022)

Epigenetics at the Intersection of COVID-19 Risk and Environmental Chemical Exposures - Catherine M. Bulka; Adam E. Enggasser; Rebecca C. Fry (Current Environmental Health Reports, 2022)

Identifying Scaling Pathways and Research Priorities for Kelp Aquaculture Nurseries Using a Techno-Economic Modeling Approach - Struan Coleman; Adam T. St. Gelais; David W. Fredriksson; Tobias Dewhurst; Damian C. Brady (Frontiers in Marine Science, 2022)
Aquaculture of seaweeds, particularly in emerging farming regions such as North America, Europe, and South America, is steadily increasing. The growth of the sector has been supported by public and private R&D investment with the long-term goal of reducing farm-gate production costs. Reducing expenses would potentially allow growers to target high volume, low value markets, such as hydrocolloids, animal feeds, food thickening agents, biofuels, and carbon dioxide removal (CDR), as well as the higher value, “whole foods” markets. Regardless of the eventual fate of farmed seaweed, nursery production must increase in parallel with ocean cultivation to support the raw materials needs of the expanding industry. We quantified S. latissima (hereafter kelp) nursery production costs and identified potential barriers to cost-effective scaling using a techno-economic model (TEM). Semi-structured interviews with nursery operators in the U.S. and Europe were supplemented by an extensive literature review to parameterize the TEM. Reducing the sporophyte grow-out duration, increasing labor capacity, de-risking energy efficient flow-through systems, and optimizing tank and PVC “spool” size emerged as the most important research priorities based on our analysis. We point towards expanded gametophyte culture, and an associated policy framework to protect wild kelp population structure from monocultures, as necessary elements to support these potential improvements. The results of this work, as well as the open-source nursery TEM, are relevant to seaweed aquaculture producers, policy makers, and researchers, and can be used to guide future decision making regarding the cost-benefit of best available nursery technology.

Dissolved organic phosphorus utilization by the marine bacterium Ruegeria pomeroyi DSS ‐3 reveals chain length‐dependent polyphosphate degradation - Jamee C. Adams; Rachel Steffen; Chau‐Wen Chou; Solange Duhamel; Julia M. Diaz (Environmental Microbiology, 2022)
Summary Dissolved organic phosphorus (DOP) is a critical nutritional resource for marine microbial communities. However, the relative bioavailability of different types of DOP, such as phosphomonoesters (P‐O‐C) and phosphoanhydrides (P‐O‐P), is poorly understood. Here we assess the utilization of these P sources by a representative bacterial copiotroph, Ruegeria pomeroyi DSS‐3. All DOP sources supported equivalent growth by R . pomeroyi , and all DOP hydrolysis rates were upregulated under phosphorus depletion (−P). A long‐chain polyphosphate (45polyP) showed the lowest hydrolysis rate of all DOP substrates tested, including tripolyphosphate (3polyP). Yet the upregulation of 45polyP hydrolysis under −P was greater than any other substrate analyzed. Proteomics revealed three common P acquisition enzymes potentially involved in polyphosphate utilization, including two alkaline phosphatases, PhoD and PhoX, and one 5′‐nucleotidase (5′‐NT). Results from DOP substrate competition experiments show that these enzymes likely have broad substrate specificities, including chain length‐dependent reactivity toward polyphosphate. These results confirm that DOP, including polyP, are bioavailable nutritional P sources for R . pomeroyi , and possibly other marine heterotrophic bacteria. Furthermore, the chain‐length dependent mechanisms, rates and regulation of polyP hydrolysis suggest that these processes may influence the composition of DOP and the overall recycling of nutrients within marine dissolved organic matter.

Predictions of biodiversity are improved by integrating trait‐based competition with abiotic filtering - Loïc Chalmandrier; Daniel B. Stouffer; Adam S. T. Purcell; William G. Lee; Andrew J. Tanentzap; Daniel C. Laughlin (Ecology Letters, 2022)
All organisms must simultaneously tolerate the environment and access limiting resources if they are to persist. Approaches to understanding abiotic filtering and competitive interactions have generally been developed independently. Consequently, integrating those factors to predict species abundances and community structure remains an unresolved challenge. We introduce a new synthetic framework that models both abiotic filtering and competition by using functional traits. First, our framework estimates species carrying capacities along abiotic gradients. Second, it estimates pairwise competitive interactions as a function of species trait differences. Applied to the study of a complex wetland community, our combined approach more than doubles the explained variance of species abundances compared to a model of abiotic tolerances alone. Trait‐based integration of competitive interactions and abiotic filtering improves our ability to predict species abundances, bringing us closer to more accurate predictions of biodiversity structure in a changing world.

Greenhouse gases and green roofs: carbon dioxide and methane fluxes in relation to substrate characteristics - Md Abdul Halim; Juliana Vantellingen; Adam S. Gorgolewski; William K. Rose; Jennifer A. P. Drake; Liat Margolis; Sean C. Thomas (Urban Ecosystems, 2022)

Is Neuroscience FAIR? A Call for Collaborative Standardisation of Neuroscience Data - Jean-Baptiste Poline; David N. Kennedy; Friedrich T. Sommer; Giorgio A. Ascoli; David C. Van Essen; Adam R. Ferguson; Jeffrey S. Grethe; Michael J. Hawrylycz; Paul M. Thompson; Russell A. Poldrack; Satrajit S. Ghosh; David B. Keator; et al. (Neuroinformatics, 2022)
In this perspective article, we consider the critical issue of data and other research object standardisation and, specifically, how international collaboration, and organizations such as the International Neuroinformatics Coordinating Facility (INCF) can encourage that emerging neuroscience data be Findable, Accessible, Interoperable, and Reusable (FAIR) . As neuroscientists engaged in the sharing and integration of multi-modal and multiscale data, we see the current insufficiency of standards as a major impediment in the Interoperability and Reusability of research results. We call for increased international collaborative standardisation of neuroscience data to foster integration and efficient reuse of research objects.

Intra-arterial blood pressure measurement: sources of error and solutions - Suresh Devasahayam; Naveen Gangadharan; C. Surekha; Bowya Baskaran; Farhan Adam Mukadam; Sathya Subramani (Medical & Biological Engineering & Computing, 2022)

Biotic factors limit the invasion of the plague pathogen ( Yersinia pestis ) in novel geographical settings - Henry G. Fell; Owen G. Osborne; Matthew D. Jones; Steve Atkinson; Simon Tarr; Suzanne H. Keddie; Adam C. Algar (Global Ecology and Biogeography, 2022)
Aim The distribution of Yersinia pestis , the pathogen that causes plague in humans, is reliant upon transmission between host species; however, the degree to which host species distributions dictate the distribution of Y. pestis , compared with limitations imposed by the environmental niche of Y. pestis per se, is debated. We test whether the present‐day environmental niche of Y. pestis differs between its native range and an invaded range and whether biotic factors (host distributions) can explain observed discrepancies. Location North America and Central Asia. Major taxa studied Yersinia pestis . Methods We use environmental niche models to determine whether the current climatic niche of Y. pestis differs between its native range in Asia and its invaded range in North America. We then test whether the inclusion of information on the distribution of host species improves the ability of models to capture the North American niche. We use geographical null models to guard against spurious correlations arising from spatially autocorrelated occurrence points. Results The current climatic niche of Y. pestis differs between its native and invaded regions. The Asian niche overpredicted the distribution of Y. pestis across North America. Including biotic factors along with the native climatic niche increased niche overlap between the native and invaded models, and models containing only biotic factors performed better than the native climatic niche alone. Geographical null models confirmed that the increased niche overlap through inclusion of biotic factors did not, with a couple of exceptions, arise solely from spatially autocorrelated occurrences. Main conclusions The current climatic niche in Central Asia differs from the current climatic niche in North America. Inclusion of biotic factors improved the fit of models to the Y. pestis distribution data in its invaded region better than climate variables alone. This highlights the importance of host species when investigating zoonotic disease introductions and suggests that climatic variables alone are insufficient to predict disease distribution in novel environments.

Who Gets the Catch? How Conventional Catch Attribution Frameworks Undermine Equity in Transboundary Fisheries - Ruth A. Davis; Quentin Hanich; Bianca Haas; Andrés M. Cisneros-Montemayor; Kamal Azmi; Katherine L. Seto; Wilf Swartz; Pedro C. González-Espinosa; Mathieu Colléter; Timothy J. H. Adams (Frontiers in Marine Science, 2022)
The focus on flag States for the purpose of attributing fisheries catch is inconsistent with the assignment of sovereign rights to coastal States under international law and undermines equity in contemporary quota allocation negotiations. We propose modernizing reporting frameworks to include zone-based reporting of fish catches to more equitably present data, ensure consistency with the Law of the Sea, and better support the realization by developing coastal States of their development aspirations consistent with SDG 14, Life Below Water. States are already required to collect the data necessary to support this change, and many regional fisheries management authorities already do so. Reforms to data collection and reporting mechanisms should support zone-based catch attribution as a central feature of negotiations around access to future fishing opportunities on shared resources. Doing so will ensure that the sovereign rights of developing coastal States are properly accounted for and implemented.

Comparison of thermochemical treatment of sewage sludge ash with sodium sulphate in laboratory-scale and pilot-scale experiments - H. Herzel; J. Stemann; S. Simon; C. Adam (International Journal of Environmental Science and Technology, 2022)
There is an ongoing debate on European scale concerning the criticality of phosphorus. In Switzerland and Germany, phosphorus recovery from phosphorus-rich waste streams will become obligatory. Sewage sludge ash is rich in phosphorus and may become an important secondary feedstock. Thermochemical treatment of sewage sludge ash with sodium sulphate under reducing conditions was shown to remove heavy metals from the solid product and produce the fully plant available crystalline phase CaNaPO 4 . Pilot-scale experiments in a rotary kiln were carried out at temperatures between 750 and 1000 °C and were compared to laboratory-scale experiments with crucibles. Process upscaling was successfully demonstrated but a series of differences were noticed: In comparison to laboratory-scale, solubility of phosphorus in samples from pilot-scale experiments was lower at all chosen treatment temperatures because of shorter retention time and incomplete decomposition of sodium sulphate. X-ray diffraction analysis revealed remaining phase fractions of whitlockite (Ca 3-x (Mg,Fe) x (PO 4 ) 2 ) and sodium sulphate from the starting materials in products and thus indicated incomplete reaction. In contrast to the results of laboratory-scale experiments, the crystalline phase CaNaPO 4 was clearly absent in the products from the rotary kiln but instead a Mg-bearing phase (Ca,Mg)NaPO 4 was formed. Laboratory-scale experiments confirmed (Ca,Mg)NaPO 4 is an intermediate phase between whitlockite and CaNaPO 4 . However, both crystalline phases are characterized by high plant availability. It was shown that heavy metal removal increased at higher temperatures whereas solubility and thus plant availability of phosphorus already reached its maxima at temperatures of 950 °C in pilot-scale and 875 °C in laboratory-scale experiments.

Effects of continuous cycling training on motor unit firing rates, input excitation, and myosin heavy chain of the vastus lateralis in sedentary females - Michael A. Trevino; Hannah L. Dimmick; Mandy E. Parra; Adam J. Sterczala; Jonathan D. Miller; Jake A. Deckert; Philip M. Gallagher; Andrew C. Fry; Joseph P. Weir; Trent J. Herda (Experimental Brain Research, 2022)

Non‐lethal sampling for the stable isotope analysis of the critically endangered European eel Anguilla anguilla : how fin and mucus compare to dorsal muscle - Rose M. Boardman; Adrian C. Pinder; Adam T. Piper; Catherine Gutmann Roberts; Rosalind M. Wright; J. Robert Britton (Journal of Fish Biology, 2022)
Ecological studies on the critically endangered European eel Anguilla anguilla often incorporate stable isotope analysis that typically uses dorsal muscle sampled from euthanised eels. To minimise the lethal sampling of imperilled populations, fin tissue and/or epidermal mucus can provide non‐lethal alternatives to muscle. The results here indicate that δ 13 C and δ 15 N values of both eel fin and mucus are not significantly different from those of muscle and can be applied directly in comparative SI studies.

Cover Image: Volume 25 Number 3, March 2022 - Joseph A. Tobias; Catherine Sheard; Alex L. Pigot; Adam J. M. Devenish; Jingyi Yang; Ferran Sayol; Montague H. C. Neate‐Clegg; Nico Alioravainen; Thomas L. Weeks; Robert A. Barber; Patrick A. Walkden; Hannah E. A. MacGregor; et al. (Ecology Letters, 2022)

AVONET: morphological, ecological and geographical data for all birds - Joseph A. Tobias; Catherine Sheard; Alex L. Pigot; Adam J. M. Devenish; Jingyi Yang; Ferran Sayol; Montague H. C. Neate‐Clegg; Nico Alioravainen; Thomas L. Weeks; Robert A. Barber; Patrick A. Walkden; Hannah E. A. MacGregor; et al. (Ecology Letters, 2022)
Functional traits offer a rich quantitative framework for developing and testing theories in evolutionary biology, ecology and ecosystem science. However, the potential of functional traits to drive theoretical advances and refine models of global change can only be fully realised when species‐level information is complete. Here we present the AVONET dataset containing comprehensive functional trait data for all birds, including six ecological variables, 11 continuous morphological traits, and information on range size and location. Raw morphological measurements are presented from 90,020 individuals of 11,009 extant bird species sampled from 181 countries. These data are also summarised as species averages in three taxonomic formats, allowing integration with a global phylogeny, geographical range maps, IUCN Red List data and the eBird citizen science database. The AVONET dataset provides the most detailed picture of continuous trait variation for any major radiation of organisms, offering a global template for testing hypotheses and exploring the evolutionary origins, structure and functioning of biodiversity.

Genetic Testing in Professional Football: Perspectives of Key Stakeholders - Alexander B. T. McAuley; David C. Hughes; Loukia G. Tsaprouni; Ian Varley; Bruce Suraci; Thomas R. Roos; Adam J. Herbert; Adam L. Kelly (Journal of Science in Sport and Exercise, 2022)
Purpose Genetic research in football is currently in it’s infancy but is growing rapidly. However, the practical application of genetic testing in football and the views concerning its use are unknown. Thus, the purpose of this study was to assess the current practical application of genetic testing in professional football and provide an insight into the perspectives of key stakeholders (i.e., coaches, practitioners, players). Methods In total, 122 participants completed an online anonymous survey. This consisted of 21 multiple choice and Likert scale questions, with the option of providing an explanation for each response. Results Findings revealed genetic testing is rarely utilised by key stakeholders (10%) or their respective organisations (14%). However, three quarters (75%) had the opinion that genetic testing will have great utility in the future. The majority (72%) believed genetic testing should be used for athlete development and injury risk, whilst 35% believed that genetic testing should be utilised for talent identification purposes. However, most key stakeholders viewed their own (89%) and their colleagues’ (79%) knowledge related to genetic testing as insufficient; mainly due to ineffective current communication methods (91%). Most believed educational workshops are required (71%), whilst nearly all (91%) were interested in developing their expertise on the utility of genetic testing. Conclusion Genetic testing is rarely used within professional football, although key stakeholders anticipate that it will be utilised more in the future. As such, educational support may prove valuable in improving key stakeholder knowledge and the practical application of genetic testing in professional football.

A natural history account of Megalomyrmex ayri Brandão, 1990 (Hymenoptera: Formicidae: Myrmicinae) - L. P. Prado; R. R. Silva; C. R. F. Brandão; M. S. C. Morini; R. M. M. Adams (Insectes Sociaux, 2022)

Mechanisms of breast cancer metastasis - S. David Nathanson; Michael Detmar; Timothy P. Padera; Lucy R. Yates; Danny R. Welch; Thomas C. Beadnell; Adam D. Scheid; Emma D. Wrenn; Kevin Cheung (Clinical & Experimental Metastasis, 2022)
Invasive breast cancer tends to metastasize to lymph nodes and systemic sites. The management of metastasis has evolved by focusing on controlling the growth of the disease in the breast/chest wall, and at metastatic sites, initially by surgery alone, then by a combination of surgery with radiation, and later by adding systemic treatments in the form of chemotherapy, hormone manipulation, targeted therapy, immunotherapy and other treatments aimed at inhibiting the proliferation of cancer cells. It would be valuable for us to know how breast cancer metastasizes; such knowledge would likely encourage the development of therapies that focus on mechanisms of metastasis and might even allow us to avoid toxic therapies that are currently used for this disease. For example, if we had a drug that targeted a gene that is critical for metastasis, we might even be able to cure a vast majority of patients with breast cancer. By bringing together scientists with expertise in molecular aspects of breast cancer metastasis, and those with expertise in the mechanical aspects of metastasis, this paper probes interesting aspects of the metastasis cascade, further enlightening us in our efforts to improve the outcome from breast cancer treatments.

Enhancing transcription in Escherichia coli and Pseudomonas putida using bacteriophage lambda anti-terminator protein Q - Jibran A. Khan; Adam M. Guss; Katy C. Kao (Biotechnology Letters, 2022)

Variation in the behaviour of an obligate corallivore is influenced by resource availability - Rachel L. Gunn; Ian R. Hartley; Adam C. Algar; N. Nadiarti; Sally A. Keith (Behavioral Ecology and Sociobiology, 2022)
Marine environments are subject to increasing disturbance events, and coral reef ecosystems are particularly vulnerable. During periods of environmental change, organisms respond initially through rapid behavioural modifications. Whilst mean population level modifications to behaviour are well documented, how these shifts vary between individuals, and the relative trade-offs that are induced, are unknown. We test whether the frequency and time invested in different behaviours varies both between and within individuals with varying resource availability. To do this, we quantify differences in four key behavioural categories (aggression, exploration, feeding and sociability) at two sites of different resource availability, using an obligate corallivore butterflyfish species ( Chaetodon lunulatus ). Individuals on a low resource site held larger territories, investing more time in exploration, which was traded off with less time invested on aggression, feeding and sociability. Repeatability measures indicated that behavioural differences between sites could plausibly be driven by both plasticity of behaviour within individuals and habitat patchiness within feeding territories. By combining population-level means, co-correlation of different behaviours and individual-level analyses, we reveal potential mechanisms behind behavioural variation in C. lunulatus due to differences in resource availability. Significance statement Using observational methods, we identify differences in the behaviour of an obligate corallivorous butterflyfish ( Chaetodon lunulatus ) between a high and a low resource site. We use a combination of density surveys, territory mapping and behavioural observation methods in a comparative analysis to relate behaviour directly to the environment in which it occurs. Bringing together insights from game theory and optimal foraging, we also use our results to highlight how understanding the correlations of different behaviours can inform our understanding of the extent to which behaviours are plastic or fixed. Furthermore, by considering how multiple behaviours are correlated, we move away from exploring individual behaviours in isolation and provide an in-depth insight into how differences in behaviour both between individuals and at the population level can affect responses to declining resource availability.

Biogeochemical consequences of a changing Arctic shelf seafloor ecosystem - Christian März; Felipe S. Freitas; Johan C. Faust; Jasmin A. Godbold; Sian F. Henley; Allyson C. Tessin; Geoffrey D. Abbott; Ruth Airs; Sandra Arndt; David K. A. Barnes; Laura J. Grange; Neil D. Gray; et al. (Ambio, 2022)
Unprecedented and dramatic transformations are occurring in the Arctic in response to climate change, but academic, public, and political discourse has disproportionately focussed on the most visible and direct aspects of change, including sea ice melt, permafrost thaw, the fate of charismatic megafauna, and the expansion of fisheries. Such narratives disregard the importance of less visible and indirect processes and, in particular, miss the substantive contribution of the shelf seafloor in regulating nutrients and sequestering carbon. Here, we summarise the biogeochemical functioning of the Arctic shelf seafloor before considering how climate change and regional adjustments to human activities may alter its biogeochemical and ecological dynamics, including ecosystem function, carbon burial, or nutrient recycling. We highlight the importance of the Arctic benthic system in mitigating climatic and anthropogenic change and, with a focus on the Barents Sea, offer some observations and our perspectives on future management and policy.

Marine phytoplankton resilience may moderate oligotrophic ecosystem responses and biogeochemical feedbacks to climate change - Adam C. Martiny; George I. Hagstrom; Tim DeVries; Robert T. Letscher; Gregory L. Britten; Catherine A. Garcia; Eric Galbraith; David Karl; Simon A. Levin; Michael W. Lomas; Allison R. Moreno; David Talmy; et al. (Limnology and Oceanography, 2022)
Are the oceans turning into deserts? Rising temperature, increasing surface stratification, and decreasing vertical inputs of nutrients are expected to cause an expansion of warm, nutrient deplete ecosystems. Such an expansion is predicted to negatively affect a trio of key ocean biogeochemical features: phytoplankton biomass, primary productivity, and carbon export. However, phytoplankton communities are complex adaptive systems with immense diversity that could render them at least partially resilient to global changes. This can be illustrated by the biology of the Prochlorococcus “collective.” Adaptations to counter stress, use of alternative nutrient sources, and frugal resource allocation can allow Prochlorococcus to buffer climate‐driven changes in nutrient availability. In contrast, cell physiology is more sensitive to temperature changes. Here, we argue that biogeochemical models need to consider the adaptive potential of diverse phytoplankton communities. However, a full understanding of phytoplankton resilience to future ocean changes is hampered by a lack of global biogeographic observations to test theories. We propose that the resilience may in fact be greater in oligotrophic waters than currently considered with implications for future predictions of phytoplankton biomass, primary productivity, and carbon export.

Evaluating the precariousness of coral recovery when coral and macroalgae are alternative basins of attraction - Russell J. Schmitt; Sally J. Holbrook; Andrew J. Brooks; Thomas C. Adam (Limnology and Oceanography, 2022)
When coral and macroalgae are alternative attractors, the trajectory of the benthic community following a major disturbance is shaped in part by whether herbivores keep macroalgae suppressed, leaving reef surfaces in a state suitable for coral colonization. Because macroalgae tend to colonize much faster than coral, an important issue is how close ambient herbivory is to the level where control of macroalgae is lost, that is, the precariousness of the coral‐invasible state relative to the switch‐point to macroalgae. In Moorea, French Polynesia, reefs dominated by the macroalga Turbinaria ornata remained so for multiple population turnovers, whereas reefs where Turbinaria was removed by a simulated pulse disturbance remained free of macroalgae throughout the 5‐yr study. A field experiment revealed that macroalgae was unable to proliferate on disturbed reefs until ambient herbivory was reduced by more than 50%. Low precariousness of the coral‐invasible state facilitated a gradual transition to a coral community because it allowed sufficient time for coral to repopulate. Accumulation of coral on disturbed reefs was 3.5 times greater than on undisturbed, Turbinaria ‐dominated reefs. Long‐term persistence of the coral‐invasible state was facilitated by high abundances of grazing herbivores that prevent the establishment of macroalgae, whereas persistence of the Turbinaria ‐dominated state reflected the scarcity of browsing herbivores that consume mature macroalgae. By revealing processes that foster bistability of coral and macroalgae states, our work helps explain the rich dynamical behavior exhibited by coral reef communities in Moorea and elsewhere.

How do fisher responses to macroalgal overgrowth influence the resilience of coral reefs? - Andrew Rassweiler; Scott D. Miller; Sally J. Holbrook; Matthew Lauer; Mark A. Strother; Sarah E. Lester; Thomas C. Adam; Jean Wencélius; Russell J. Schmitt (Limnology and Oceanography, 2022)
Many coral reefs have shifted from coral‐ to macroalgae‐dominated community states, heightening the need to understand resilience of coral communities. Fishing on herbivores often reduces resilience of the coral state, as lower herbivory fosters macroalgal establishment. Despite the acknowledged importance of fishing, relatively little attention has been paid to how fishers change their behavior as macroalgae overgrow reefs, or how the resulting dynamic feedbacks might affect resilience. We address these questions in Moorea, French Polynesia, where local fishers target herbivorous fishes and where shifts to algal dominance have occurred on some lagoon reefs. We quantified fisher preferences for reef habitats where they target various taxa. For the two most ecologically important taxa of herbivores targeted in the fishery, parrotfish (Scaridae) and unicornfish ( Naso ), fishers preferred to harvest from locations with less macroalgae. We incorporated these habitat preferences into a spatially explicit social–ecological model of reef dynamics to explore consequences of changes in fishing behavior for resilience of the coral state, particularly following disturbance. Fishing that targets low‐macroalgae locations typically generates resilience by facilitating local recovery of herbivores and thus of coral in the less‐targeted macroalgae‐dominated patches. However, the resulting movement of fishers across the seascape can sometimes create fragility; if coral loss is widespread, avoidance of macroalgae concentrates fishing in patches having the highest coral cover, resulting in loss of coral via reduced herbivory. Our results emphasize that resilience and coral‐macroalgae regime shifts cannot be understood without considering humans as a dynamic part of the system.

Size‐dependent mortality of corals during marine heatwave erodes recovery capacity of a coral reef - Kelly E. Speare; Thomas C. Adam; Erin M. Winslow; Hunter S. Lenihan; Deron E. Burkepile (Global Change Biology, 2022)
For many long‐lived taxa, such as trees and corals, older, and larger individuals often have the lowest mortality and highest fecundity. However, climate change‐driven disturbances such as droughts and heatwaves may fundamentally alter typical size‐dependent patterns of mortality and reproduction in these important foundation taxa. Working in Moorea, French Polynesia, we investigated how a marine heatwave in 2019, one of the most intense marine heatwaves at our sites over the past 30 years, drove patterns of coral bleaching and mortality. The marine heatwave drove island‐wide mass coral bleaching that killed up to 76% and 65% of the largest individuals of the two dominant coral genera, Pocillopora and Acropora , respectively. Colonies of Pocillopora and Acropora ≥30 cm diameter were ~3.5× and ~1.3×, respectively, more likely to die than colonies 60% because big corals are disproportionately important for reproduction on reefs. Additionally, the survivorship of microscopic coral recruits, critical for the recovery of corals following disturbances, declined to 2%, over an order of magnitude lower compared to a year without elevated thermal stress, where 33% of coral recruits survived. While other research has shown that larger corals can bleach more frequently than smaller corals, we show the severe impact this phenomenon can have at the reef‐wide scale. As marine heatwaves become more frequent and intense, disproportionate mortality of the largest, most fecund corals and near‐complete loss of entire cohorts of newly‐settled coral recruits will likely reduce the recovery capacity of these iconic ecosystems.

Changes in tree drought sensitivity provided early warning signals to the California drought and forest mortality event - Rachel M. Keen; Steven L. Voelker; S.‐Y. Simon Wang; Barbara J. Bentz; Michael L. Goulden; Cody R. Dangerfield; Charlotte C. Reed; Sharon M. Hood; Adam Z. Csank; Todd E. Dawson; Andrew G. Merschel; Christopher J. Still (Global Change Biology, 2022)
Climate warming in recent decades has negatively impacted forest health in the western United States. Here, we report on potential early warning signals (EWS) for drought‐related mortality derived from measurements of tree‐ring growth (ring width index; RWI) and carbon isotope discrimination (∆ 13 C), primarily focused on ponderosa pine ( Pinus ponderosa ). Sampling was conducted in the southern Sierra Nevada Mountains, near the epicenter of drought severity and mortality associated with the 2012–2015 California drought and concurrent outbreak of western pine beetle ( Dendroctonus brevicomis ). At this site, we found that widespread mortality was presaged by five decades of increasing sensitivity (i.e., increased explained variation) of both tree growth and ∆ 13 C to Palmer Drought Severity Index (PDSI). We hypothesized that increasing sensitivity of tree growth and ∆ 13 C to hydroclimate constitute EWS that indicate an increased likelihood of widespread forest mortality caused by direct and indirect effects of drought. We then tested these EWS in additional ponderosa pine‐dominated forests that experienced varying mortality rates associated with the same California drought event. In general, drier sites showed increasing sensitivity of RWI to PDSI over the last century, as well as higher mortality following the California drought event compared to wetter sites. Two sites displayed evidence that thinning or fire events that reduced stand basal area effectively reversed the trend of increasing hydroclimate sensitivity. These comparisons indicate that reducing competition for soil water and/or decreasing bark beetle host tree density via forest management—particularly in drier regions—may buffer these forests against drought stress and associated mortality risk. EWS such as these could provide land managers more time to mitigate the extent or severity of forest mortality in advance of droughts. Substantial efforts at deploying additional dendrochronological research in concert with remote sensing and forest modeling will aid in forecasting of forest responses to continued climate warming.

An introduction to decision science for conservation - Victoria Hemming; Abbey E. Camaclang; Megan S. Adams; Mark Burgman; Katherine Carbeck; Josie Carwardine; Iadine Chadès; Lia Chalifour; Sarah J. Converse; Lindsay N. K. Davidson; Georgia E. Garrard; Riley Finn; et al. (Conservation Biology, 2022)
Biodiversity conservation decisions are difficult, especially when they involve differing values, complex multidimensional objectives, scarce resources, urgency, and considerable uncertainty. Decision science embodies a theory about how to make difficult decisions and an extensive array of frameworks and tools that make that theory practical. We sought to improve conceptual clarity and practical application of decision science to help decision makers apply decision science to conservation problems. We addressed barriers to the uptake of decision science, including a lack of training and awareness of decision science; confusion over common terminology and which tools and frameworks to apply; and the mistaken impression that applying decision science must be time consuming, expensive, and complex. To aid in navigating the extensive and disparate decision science literature, we clarify meaning of common terms: decision science , decision theory , decision analysis , structured decision‐making , and decision‐support tools . Applying decision science does not have to be complex or time consuming; rather, it begins with knowing how to think through the components of a decision utilizing decision analysis (i.e., define the problem, elicit objectives, develop alternatives, estimate consequences, and perform trade‐offs). This is best achieved by applying a rapid‐prototyping approach. At each step, decision‐support tools can provide additional insight and clarity, whereas decision‐support frameworks (e.g., priority threat management and systematic conservation planning) can aid navigation of multiple steps of a decision analysis for particular contexts. We summarize key decision‐support frameworks and tools and describe to which step of a decision analysis, and to which contexts, each is most useful to apply. Our introduction to decision science will aid in contextualizing current approaches and new developments, and help decision makers begin to apply decision science to conservation problems.

Corrigendum to: Leveraging Administrative Capacity to Manage Landscape-Scale, Cross-Boundary Disturbance in the Black Hills: What Roles for Federal, State, Local, and Non-Governmental Partners? - M M Steen-Adams; J B Abrams; H R Huber-Stearns; C Bone; C Moseley (Journal of Forestry, 2022)

Leveraging Administrative Capacity to Manage Landscape-Scale, Cross-Boundary Disturbance in the Black Hills: What Roles for Federal, State, Local, and Nongovernmental Partners? - M M Steen-Adams; J B Abrams; H R Huber-Stearns; C Bone; C Moseley (Journal of Forestry, 2022)
Public land management agencies, such as the USDA Forest Service (USFS), confront challenges in leveraging limited administrative capacity to effectively manage landscape-scale, cross-boundary disturbances. Using case study methods, we investigated the ~1996–2016 outbreak of mountain pine beetle (Dendroctonus ponderosae Hopkins) in the Black Hills, a 1.5 million acre region in South Dakota and Wyoming. We identified four practices that can promote the leveraging of administrative capacity: (1) establishing multilevel governance networks; (2) performing both distinct and shared (“redundant”) partner roles; (3) maximizing the collaborative toolbox: drawing upon the variety of policy tools (older, newer) and modes of use (conventional, experimental); and (4) engaging a multilevel, multiorganizational network, with attention to the ways that a variety of network linkages can deploy a variety of policy tool types. This case demonstrates how the government-led model of network governance can be applied to leverage administrative capacity. These findings point to strategies to promote landscape-scale, cross-boundary management.

Promoting FAIR Data Through Community-driven Agile Design: the Open Data Commons for Spinal Cord Injury (odc-sci.org) - Abel Torres-Espín; Carlos A. Almeida; Austin Chou; J. Russell Huie; Michael Chiu; Romana Vavrek; Jeff Sacramento; Michael B. Orr; John C. Gensel; Jeffery S. Grethe; Maryann E. Martone; Karim Fouad; et al. (Neuroinformatics, 2022)
The past decade has seen accelerating movement from data protectionism in publishing toward open data sharing to improve reproducibility and translation of biomedical research. Developing data sharing infrastructures to meet these new demands remains a challenge. One model for data sharing involves simply attaching data, irrespective of its type, to publisher websites or general use repositories. However, some argue this creates a ‘data dump’ that does not promote the goals of making data Findable, Accessible, Interoperable and Reusable (FAIR). Specialized data sharing communities offer an alternative model where data are curated by domain experts to make it both open and FAIR. We report on our experiences developing one such data-sharing ecosystem focusing on ‘long-tail’ preclinical data, the Open Data Commons for Spinal Cord Injury (odc-sci.org). ODC-SCI was developed with community-based agile design requirements directly pulled from a series of workshops with multiple stakeholders (researchers, consumers, non-profit funders, governmental agencies, journals, and industry members). ODC-SCI focuses on heterogeneous tabular data collected by preclinical researchers including bio-behaviour, histopathology findings and molecular endpoints. This has led to an example of a specialized neurocommons that is well-embraced by the community it aims to serve. In the present paper, we provide a review of the community-based design template and describe the adoption by the community including a high-level review of current data assets, publicly released datasets, and web analytics. Although odc-sci.org is in its late beta stage of development, it represents a successful example of a specialized data commons that may serve as a model for other fields.

Excavating FAIR Data: the Case of the Multicenter Animal Spinal Cord Injury Study (MASCIS), Blood Pressure, and Neuro-Recovery - Carlos A. Almeida; Abel Torres-Espin; J. Russell Huie; Dongming Sun; Linda J. Noble-Haeusslein; Wise Young; Michael S. Beattie; Jacqueline C. Bresnahan; Jessica L. Nielson; Adam R. Ferguson (Neuroinformatics, 2022)
Meta-analyses suggest that the published literature represents only a small minority of the total data collected in biomedical research, with most becoming ‘dark data’ unreported in the literature. Dark data is due to publication bias toward novel results that confirm investigator hypotheses and omission of data that do not. Publication bias contributes to scientific irreproducibility and failures in bench-to-bedside translation. Sharing dark data by making it Findable, Accessible, Interoperable, and Reusable (FAIR) may reduce the burden of irreproducible science by increasing transparency and support data-driven discoveries beyond the lifecycle of the original study. We illustrate feasibility of dark data sharing by recovering original raw data from the Multicenter Animal Spinal Cord Injury Study (MASCIS), an NIH-funded multi-site preclinical drug trial conducted in the 1990s that tested efficacy of several therapies after a spinal cord injury (SCI). The original drug treatments did not produce clear positive results and MASCIS data were stored in boxes for more than two decades. The goal of the present study was to independently confirm published machine learning findings that perioperative blood pressure is a major predictor of SCI neuromotor outcome (Nielson et al., 2015). We recovered, digitized, and curated the data from 1125 rats from MASCIS. Analyses indicated that high perioperative blood pressure at the time of SCI is associated with poorer health and worse neuromotor outcomes in more severe SCI, whereas low perioperative blood pressure is associated with poorer health and worse neuromotor outcome in moderate SCI. These findings confirm and expand prior results that a narrow window of blood-pressure control optimizes outcome, and demonstrate the value of recovering dark data for assessing reproducibility of findings with implications for precision therapeutic approaches.

Influence of watershed suburbanization on leaf litter decomposition and microbial activity - Adam S. Wymore; Anna Bourakovsky; Hannah M. Fazekas; Jane C. Marks; William H. McDowell (Hydrobiologia, 2022)

Common, low-frequency, rare, and ultra-rare coding variants contribute to COVID-19 severity - Chiara Fallerini; Nicola Picchiotti; Margherita Baldassarri; Kristina Zguro; Sergio Daga; Francesca Fava; Elisa Benetti; Sara Amitrano; Mirella Bruttini; Maria Palmieri; Susanna Croci; Mirjam Lista; et al. (Human Genetics, 2022)
The combined impact of common and rare exonic variants in COVID-19 host genetics is currently insufficiently understood. Here, common and rare variants from whole-exome sequencing data of about 4000 SARS-CoV-2-positive individuals were used to define an interpretable machine-learning model for predicting COVID-19 severity. First, variants were converted into separate sets of Boolean features, depending on the absence or the presence of variants in each gene. An ensemble of LASSO logistic regression models was used to identify the most informative Boolean features with respect to the genetic bases of severity. The Boolean features selected by these logistic models were combined into an Integrated PolyGenic Score that offers a synthetic and interpretable index for describing the contribution of host genetics in COVID-19 severity, as demonstrated through testing in several independent cohorts. Selected features belong to ultra-rare, rare, low-frequency, and common variants, including those in linkage disequilibrium with known GWAS loci. Noteworthily, around one quarter of the selected genes are sex-specific. Pathway analysis of the selected genes associated with COVID-19 severity reflected the multi-organ nature of the disease. The proposed model might provide useful information for developing diagnostics and therapeutics, while also being able to guide bedside disease management.

Variation in Densities of the Salt Marsh Katydid Orchelimum fidicinium over Space and Time - Tianjiao Adams; Huy D. Vu; Steven C. Pennings (Estuaries and Coasts, 2022)

US hysterectomy prevalence by age, race and ethnicity from BRFSS and NHIS: implications for analyses of cervical and uterine cancer rates - Emily E. Adam; Mary C. White; Mona Saraiya (Cancer Causes & Control, 2022)

An Early Pleistocene hippopotamus from Westbury Cave, Somerset, England: support for a previously unrecognized temperate interval in the British Quaternary record - Neil F. Adams; Ian Candy; Danielle C. Schreve (Journal of Quaternary Science, 2022)
Although fossil assemblages from the late Early Pleistocene are very rare in Britain, the site of Westbury Cave in Somerset, England, has the potential to address this gap. The mammal fossils recovered previously from the Siliceous Member in Westbury Cave, though few in number, have hinted at an age for the deposits that is as yet unparalleled in Britain. Here, we describe the first bona fide occurrence of Hippopotamus in the British Early Pleistocene, discovered during recent reinvestigation of the Siliceous Member. The hippo fossil indicates a refined biochronological age of ca. 1.5–1.07 Ma for the Siliceous Member and a palaeoclimate that was warm and humid, which accords well with previous palaeoenvironmental inferences. A synthesis of late Early Pleistocene hippo occurrences suggests that the Siliceous Member hippo may have been part of an early colonization of north‐west Europe by these megaherbivores, possibly during MIS (Marine Oxygen Isotope Stage) 31. Alternatively, it evidences a currently cryptic northward migration during an even earlier temperate phase. In either case, the Siliceous Member is likely to represent a warm period that has not been recognized previously in the British Quaternary record.

Interannual climate variability mediates changes in carbon and nitrogen pools caused by annual grass invasion in a semiarid shrubland - Adam L. Mahood; Rachel O. Jones; David I. Board; Jennifer K. Balch; Jeanne C. Chambers (Global Change Biology, 2022)
Exotic plant invasions alter ecosystem properties and threaten ecosystem functions globally. Interannual climate variability (ICV) influences both plant community composition (PCC) and soil properties, and interactions between ICV and PCC may influence nitrogen (N) and carbon (C) pools. We asked how ICV and non‐native annual grass invasion covary to influence soil and plant N and C in a semiarid shrubland undergoing widespread ecosystem transformation due to invasions and altered fire regimes. We sampled four progressive stages of annual grass invasion at 20 sites across a large (25,000 km 2 ) landscape for plant community composition, plant tissue N and C, and soil total N and C in 2013 and 2016, which followed 2 years of dry and wet conditions, respectively. Multivariate analyses and ANOVAs showed that in invasion stages where native shrub and perennial grass and forb communities were replaced by annual grass‐dominated communities, the ecosystem lost more soil N and C in wet years. Path analysis showed that high water availability led to higher herbaceous cover in all invasion stages. In stages with native shrubs and perennial grasses, higher perennial grass cover was associated with increased soil C and N, while in annual‐dominated stages, higher annual grass cover was associated with losses of soil C and N. Also, soil total C and C:N ratios were more homogeneous in annual‐dominated invasion stages as indicated by within‐site standard deviations. Loss of native shrubs and perennial grasses and forbs coupled with annual grass invasion may lead to long‐term declines in soil N and C and hamper restoration efforts. Restoration strategies that use innovative techniques and novel species to address increasing temperatures and ICV and emphasize maintaining plant community structure—shrubs, grasses, and forbs—will allow sagebrush ecosystems to maintain C sequestration, soil fertility, and soil heterogeneity.

Response of Antarctic soil fauna to climate‐driven changes since the Last Glacial Maximum - André L. C. Franco; Byron J. Adams; Melisa A. Diaz; Nathan P. Lemoine; Nicholas B. Dragone; Noah Fierer; W. Berry Lyons; Ian Hogg; Diana H. Wall (Global Change Biology, 2022)
Understanding how terrestrial biotic communities have responded to glacial recession since the Last Glacial Maximum (LGM) can inform present and future responses of biota to climate change. In Antarctica, the Transantarctic Mountains (TAM) have experienced massive environmental changes associated with glacial retreat since the LGM, yet we have few clues as to how its soil invertebrate‐dominated animal communities have responded. Here, we surveyed soil invertebrate fauna from above and below proposed LGM elevations along transects located at 12 features across the Shackleton Glacier region. Our transects captured gradients of surface ages possibly up to 4.5 million years and the soils have been free from human disturbance for their entire history. Our data support the hypothesis that soils exposed during the LGM are now less suitable habitats for invertebrates than those that have been exposed by deglaciation following the LGM. Our results show that faunal abundance, community composition, and diversity were all strongly affected by climate‐driven changes since the LGM. Soils more recently exposed by the glacial recession (as indicated by distances from present ice surfaces) had higher faunal abundances and species richness than older exposed soils. Higher abundances of the dominant nematode Scottnema were found in older exposed soils, while Eudorylaimus , Plectus , tardigrades, and rotifers preferentially occurred in more recently exposed soils. Approximately 30% of the soils from which invertebrates could be extracted had only Scottnema , and these single‐taxon communities occurred more frequently in soils exposed for longer periods of time. Our structural equation modeling of abiotic drivers highlighted soil salinity as a key mediator of Scottnema responses to soil exposure age. These changes in soil habitat suitability and biotic communities since the LGM indicate that Antarctic terrestrial biodiversity throughout the TAM will be highly altered by climate warming.

Habitat value of bivalve shellfish and seaweed aquaculture for fish and invertebrates: Pathways, synthesis and next steps - Seth J. Theuerkauf; Luke T. Barrett; Heidi K. Alleway; Barry A. Costa‐Pierce; Adam St. Gelais; Robert C. Jones (Reviews in Aquaculture, 2022)
Aquaculture can have negative environmental impacts, adding to the suite of anthropogenic stressors that challenge coastal ecosystems. However, a growing body of scientific evidence indicates that the commercial cultivation of bivalve shellfish and seaweed can deliver valuable ecosystem goods and services, including provision of new habitats for fish and mobile invertebrate species. We completed a systematic literature review of studies focused on understanding habitat‐related interactions associated with bivalve and seaweed aquaculture, and a brief meta‐analysis of 65 studies to evaluate fish and mobile macroinvertebrate populations at farms and reference sites. Bivalve and seaweed aquaculture were associated with higher abundance ( n = 59, range: 0.05× to 473×, median lnRR = 0.67) and species richness ( n = 29, range: 0.68× to 4.3×, median lnRR = 0.13) of wild, mobile macrofauna. Suspended or elevated mussel and oyster culture yielded the largest increases in wild macrofaunal abundance and species richness. We describe the major mechanisms and pathways by which bivalve and seaweed aquaculture may positively influence the structure and function of faunal communities—including provision of structured habitat, provision of food resources and enhanced reproduction and recruitment—and identify the role of the species cultivated and cultivation gear in affecting habitat value. Given the continued deterioration of coastal habitats and increasing investments into their restoration, understanding how industry activities such as aquaculture can be designed to deliver food within ecological limits and have positive influences on ecosystem goods and services is essential in ensuring ecological, social and economic objectives can be achieved.

Reproductive patterns of solitary cavity-nesting bees responsive to both local and landscape factors - Aaron N. Sexton; Sarah Benton; Adam C. Browning; Sarah M. Emery (Urban Ecosystems, 2021)

Hotter droughts alter resource allocation to chemical defenses in piñon pine - Amy M. Trowbridge; Henry D. Adams; Adam Collins; Lee Turin Dickman; Charlotte Grossiord; Megan Hofland; Shealyn Malone; David K. Weaver; Sanna Sevanto; Paul C. Stoy; Nate G. McDowell (Oecologia, 2021)
Heat and drought affect plant chemical defenses and thereby plant susceptibility to pests and pathogens. Monoterpenes are of particular importance for conifers as they play critical roles in defense against bark beetles. To date, work seeking to understand the impacts of heat and drought on monoterpenes has primarily focused on young potted seedlings, leaving it unclear how older age classes that are more vulnerable to bark beetles might respond to stress. Furthermore, we lack a clear picture of what carbon resources might be prioritized to support monoterpene synthesis under drought stress. To address this, we measured needle and woody tissue monoterpene concentrations and physiological variables simultaneously from mature piñon pines ( Pinus edulis ) from a unique temperature and drought manipulation field experiment. While heat had no effect on total monoterpene concentrations, trees under combined heat and drought stress exhibited ~ 85% and 35% increases in needle and woody tissue, respectively, over multiple years. Plant physiological variables like maximum photosynthesis each explained less than 10% of the variation in total monoterpenes for both tissue types while starch and glucose + fructose measured 1-month prior explained ~ 45% and 60% of the variation in woody tissue total monoterpene concentrations. Although total monoterpenes increased under combined stress, some key monoterpenes with known roles in bark beetle ecology decreased. These shifts may make trees more favorable for bark beetle attack rather than well defended, which one might conclude if only considering total monoterpene concentrations. Our results point to cumulative and synergistic effects of heat and drought that may reprioritize carbon allocation of specific non-structural carbohydrates toward defense.

Establishment of a microsatellite genetic baseline for North American Atlantic sturgeon (Acipenser o. oxyrhinchus) and range-wide analysis of population genetics - Shannon L. White; David C. Kazyak; Tanya L. Darden; Daniel J. Farrae; Barbara A. Lubinski; Robin L. Johnson; Michael S. Eackles; Matthew T. Balazik; Harold M. Brundage; Adam G. Fox; Dewayne A. Fox; Christian H. Hager; et al. (Conservation Genetics, 2021)

Associations of alcohol use, HIV infection, and age with brain white matter microstructure - Mollie A. Monnig; Joseph M. Gullett; Eric C. Porges; Adam J. Woods; Peter M. Monti; Karen Tashima; Neda Jahanshad; Paul Thompson; Talia Nir; Ronald A. Cohen (Journal of NeuroVirology, 2021)

Correction to: 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales - Jens H. Kuhn; Scott Adkins; Bernard R. Agwanda; Rim Al Kubrusli; Sergey V. Alkhovsky; Gaya K. Amarasinghe; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Christopher F. Basler; et al. (Archives of Virology, 2021)

2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales - Jens H. Kuhn; Scott Adkins; Bernard R. Agwanda; Rim Al Kubrusli; Sergey V. Alkhovsky; Gaya K. Amarasinghe; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Christopher F. Basler; et al. (Archives of Virology, 2021)

Diverse but uncertain responses of picophytoplankton lineages to future climate change - Pedro Flombaum; Adam C. Martiny (Limnology and Oceanography, 2021)
Climate change is projected to modify the physical and chemical environment of the ocean, but the quantitative impact on the distribution of phytoplankton groups is unclear. Most Earth System Models (ESMs) predict future declines of phytoplankton in low latitude waters, contradicting observations showing that picophytoplankton can reach high abundance in warm waters. Here, we used a historic and three climate scenarios along with quantitative niche models to project Prochlorococcus , Synechococcus , and picoeukaryotic phytoplankton distributions for the year 2100. First, we found global responses with up to 50% and 9% increase for Prochlorococcus and Synechococcus abundances, respectively, and 8% decrease for picoeukaryotic phytoplankton. All groups increased in abundance at low latitude, and Synechococcus and picoeukaryotic phytoplankton showed bands of decreases and increases in mid‐ and high‐latitudes, respectively. Prochlorococcus temporal trends were consistent among ESMs and increased with the strength of the scenario, while Synechococcus and picoeukaryotic phytoplankton showed mixed results. Second, we evaluated sources of uncertainty associated to future projections. The anthropogenic uncertainty, associated to climate scenarios, increased with time and was relevant for Prochlorococcus . The environmental and biological uncertainty, associated to ESMs and niche models, respectively, represented the largest fraction but differed among lineages. Quantifying uncertainties is key because the predicted differences in the future distribution and abundance can have large‐scale consequences on ocean ecosystem functioning.

Improving species status assessments under the U.S. Endangered Species Act and implications for multispecies conservation challenges worldwide - Reed F. Noss; Jennifer M. Cartwright; Dwayne Estes; Theo Witsell; Gregg Elliott; Daniel Adams; Matthew Albrecht; Ryan Boyles; Patrick Comer; Chris Doffitt; Don Faber‐Langendoen; JoVonn Hill; et al. (Conservation Biology, 2021)
Despite its successes, the U.S. Endangered Species Act (ESA) has proven challenging to implement due to funding limitations, workload backlog, and other problems. As threats to species survival intensify and as more species come under threat, the need for the ESA and similar conservation laws and policies in other countries to function efficiently has grown. Attempts by the U.S. Fish and Wildlife Service (USFWS) to streamline ESA decisions include multispecies recovery plans and habitat conservation plans. We address species status assessment (SSA), a USFWS process to inform ESA decisions from listing to recovery, within the context of multispecies and ecosystem planning. Although existing SSAs have a single‐species focus, ecosystem‐based research can efficiently inform multiple SSAs within a region and provide a foundation for transition to multispecies SSAs in the future. We considered at‐risk grassland species and ecosystems within the southeastern United States, where a disproportionate number of rare and endemic species are associated with grasslands. To initiate our ecosystem‐based approach, we used a combined literature‐based and structured World Café workshop format to identify science needs for SSAs. Discussions concentrated on 5 categories of threats to grassland species and ecosystems, consistent with recommendations to make shared threats a focus of planning under the ESA: (1) habitat loss, fragmentation, and disruption of functional connectivity; (2) climate change; (3) altered disturbance regimes; (4) invasive species; and (5) localized impacts. For each threat, workshop participants identified science and information needs, including database availability, research priorities, and modeling and mapping needs. Grouping species by habitat and shared threats can make the SSA process and other planning processes for conservation of at‐risk species worldwide more efficient and useful. We found a combination of literature review and structured discussion effective for identifying the scientific information and analysis needed to support the development of multiple SSAs. Article impact statement : Species status assessments can be improved by an ecosystem‐based approach that groups imperiled species by shared habitats and threats.

Bridging research and practice in conservation - Andrew N. Kadykalo; Rachel T. Buxton; Peter Morrison; Christine M. Anderson; Holly Bickerton; Charles M. Francis; Adam C. Smith; Lenore Fahrig (Conservation Biology, 2021)
Calls for biodiversity conservation practice to be more evidence based are growing, and we agree evidence use in conservation practice needs improvement. However, evidence‐based conservation will not be realized without improved access to evidence. In medicine, unlike in conservation, a well‐established and well‐funded layer of intermediary individuals and organizations engage with medical practitioners, synthesize primary research relevant to decision making, and make evidence easily accessible. These intermediaries prepare targeted evidence summaries and distribute them to practitioners faced with time‐sensitive and value‐laden decisions. To be effective, these intermediaries, who we refer to as evidence bridges , should identify research topics based on the priorities of practitioners; synthesize evidence; prepare and distribute easy‐to‐find and easy‐to‐use evidence summaries; and develop and maintain networks of connections with researchers and practitioners. Based on a review of the literature regarding evidence intermediaries in conservation and environmental management, as well as an anonymous questionnaire searching for such organizations, we found few intermediaries that met all these criteria. Few evidence bridges that do exist are unable to reach most conservation practitioners, which include resource managers in government and industry, conservation organizations, and farmers and other private landowners. We argue that the lack of evidence bridges from research to practitioners contributes to evidence complacency and limits the use of evidence in conservation action. Nevertheless, several existing organizations help reduce the gap between evidence and practice and could serve as a foundation for building additional components of evidence bridges in conservation. Although evidence bridges need expertise in research and evidence synthesis, they also require expertise in identifying and communicating with the community of practitioners most in need of clear and concise syntheses of evidence. Article Impact Statement: Evidence‐based conservation will not be realized without improved access to evidence. We call for intermediary evidence bridges.

Identification of aneuploidy in dogs screened by a SNP microarray - Lisa G. Shaffer; Bradley Hopp; Marek Switonski; Adam Zahand; Blake C. Ballif (Human Genetics, 2021)

Wealth and urbanization shape medium and large terrestrial mammal communities - Seth B. Magle; Mason Fidino; Heather A. Sander; Adam T. Rohnke; Kelli L. Larson; Travis Gallo; Cria A. M. Kay; Elizabeth W. Lehrer; Maureen H. Murray; Solny A. Adalsteinsson; Adam A. Ahlers; Whitney J. B. Anthonysamy; et al. (Global Change Biology, 2021)
Urban biodiversity provides critical ecosystem services and is a key component to environmentally and socially sustainable cities. However, biodiversity varies greatly within and among cities, leading to human communities with changing and unequal experiences with nature. The “luxury effect,” a hypothesis that predicts a positive correlation between wealth, typically measured by per capita income, and species richness may be one indication of these inequities. While the luxury effect is well studied for some taxa, it has rarely been investigated for mammals, which provide unique ecosystem services (e.g., biological pest control) and exhibit significant potential for negative human–wildlife interactions (e.g., nuisances or conflicts). We analyzed a large dataset of mammal detections across 20 North American cities to test whether the luxury effect is consistent for medium‐ to large‐sized terrestrial mammals across diverse urban contexts. Overall, support for the luxury effect, as indicated by per capita income, was inconsistent; we found evidence of a luxury effect in approximately half of our study cities. Species richness was, however, highly and negatively correlated with urban intensity in most cities. We thus suggest that economic factors play an important role in shaping urban mammal communities for some cities and species, but that the strongest driver of urban mammal diversity is urban intensity. To better understand the complexity of urban ecosystems, ecologists and social scientists must consider the social and political factors that drive inequitable human experiences with nature in cities.

ASL expression in ALDH1A1+ neurons in the substantia nigra metabolically contributes to neurodegenerative phenotype - Shaul Lerner; Raya Eilam; Lital Adler; Julien Baruteau; Topaz Kreiser; Michael Tsoory; Alexander Brandis; Tevie Mehlman; Mina Ryten; Juan A. Botia; Sonia Garcia Ruiz; Alejandro Cisterna Garcia; et al. (Human Genetics, 2021)
Argininosuccinate lyase (ASL) is essential for the NO-dependent regulation of tyrosine hydroxylase (TH) and thus for catecholamine production. Using a conditional mouse model with loss of ASL in catecholamine neurons, we demonstrate that ASL is expressed in dopaminergic neurons in the substantia nigra pars compacta, including the ALDH1A1 + subpopulation that is pivotal for the pathogenesis of Parkinson disease (PD). Neuronal loss of ASL results in catecholamine deficiency, in accumulation and formation of tyrosine aggregates, in elevation of α-synuclein, and phenotypically in motor and cognitive deficits. NO supplementation rescues the formation of aggregates as well as the motor deficiencies. Our data point to a potential metabolic link between accumulations of tyrosine and seeding of pathological aggregates in neurons as initiators for the pathological processes involved in neurodegeneration. Hence, interventions in tyrosine metabolism via regulation of NO levels may be therapeutic beneficial for the treatment of catecholamine-related neurodegenerative disorders.

Correction to: Chronic low-level nutrient enrichment benefits coral thermal performance in a fore reef habitat - Danielle M. Becker; Hollie M. Putnam; Deron E. Burkepile; Thomas C. Adam; Rebecca Vega Thurber; Nyssa J. Silbiger (Coral Reefs, 2021)

Chronic low-level nutrient enrichment benefits coral thermal performance in a fore reef habitat - Danielle M. Becker; Hollie M. Putnam; Deron E. Burkepile; Thomas C. Adam; Rebecca Vega Thurber; Nyssa J. Silbiger (Coral Reefs, 2021)

Association of remote mild traumatic brain injury with cortical amyloid burden in clinically normal older adults - Breton M. Asken; William G. Mantyh; Renaud La Joie; Amelia Strom; Kaitlin B. Casaletto; Adam M. Staffaroni; Alexandra C. Apple; Cutter A. Lindbergh; Leonardo Iaccarino; Michelle You; Harli Grant; Corrina Fonseca; et al. (Brain Imaging and Behavior, 2021)

Local Treatment of Non-small Cell Lung Cancer with a Spray-Dried Bevacizumab Formulation - Kimberly B. Shepard; David T. Vodak; Philip J. Kuehl; David Revelli; Yue Zhou; Amanda M. Pluntze; Molly S. Adam; Julia C. Oddo; Lauren Switala; Jonathan L. Cape; John M. Baumann; Michael Banks (AAPS PharmSciTech, 2021)
Local delivery of biotherapeutics to the lung holds great promise for treatment of lung diseases, but development of physically stable, biologically active dry powder formulations of large molecules for inhalation has remained a challenge. Here, spray drying was used to manufacture a dry powder pulmonary formulation of bevacizumab, a monoclonal antibody approved to treat non-small cell lung cancer (NSCLC) by intravenous infusion. By reformulating bevacizumab for local delivery, reduced side effects, lower doses, and improved patient compliance are possible. The formulation had aerosol properties suitable for delivery to the deep lung, as well as good physical stability at ambient temperature for at least 6 months. Bevacizumab’s anti-VEGF bioactivity was not impacted by the manufacturing process. The formulation was efficacious in an in vivo rat model for NSCLC at a 10-fold decrease in dose relative to the intravenous control.

Body size and digestive system shape resource selection by ungulates: A cross‐taxa test of the forage maturation hypothesis - Saeideh Esmaeili; Brett R. Jesmer; Shannon E. Albeke; Ellen O. Aikens; Kathryn A. Schoenecker; Sarah R. B. King; Briana Abrahms; Bayarbaatar Buuveibaatar; Jeffrey L. Beck; Randall B. Boone; Francesca Cagnacci; Simon Chamaillé‐Jammes; et al. (Ecology Letters, 2021)
The forage maturation hypothesis (FMH) states that energy intake for ungulates is maximised when forage biomass is at intermediate levels. Nevertheless, metabolic allometry and different digestive systems suggest that resource selection should vary across ungulate species. By combining GPS relocations with remotely sensed data on forage characteristics and surface water, we quantified the effect of body size and digestive system in determining movements of 30 populations of hindgut fermenters (equids) and ruminants across biomes. Selection for intermediate forage biomass was negatively related to body size, regardless of digestive system. Selection for proximity to surface water was stronger for equids relative to ruminants, regardless of body size. To be more generalisable, we suggest that the FMH explicitly incorporate contingencies in body size and digestive system, with small‐bodied ruminants selecting more strongly for potential energy intake, and hindgut fermenters selecting more strongly for surface water.

Advancing the Interpretation of Shallow Water Marine Soundscapes - Megan F. McKenna; Simone Baumann-Pickering; Annebelle C. M. Kok; William K. Oestreich; Jeffrey D. Adams; Jack Barkowski; Kurt M. Fristrup; Jeremy A. Goldbogen; John Joseph; Ella B. Kim; Anke Kügler; Marc O. Lammers; et al. (Frontiers in Marine Science, 2021)
Soundscapes offer rich descriptions of composite acoustic environments. Characterizing marine soundscapes simply through sound levels results in incomplete descriptions, limits the understanding of unique features, and impedes meaningful comparisons. Sources that contribute to sound level metrics shift in time and space with changes in biological patterns, physical forces, and human activity. The presence of a constant or chronic source is often interwoven with episodic sounds. Further, the presence and intensity of sources can influence other sources, calling for a more integrated approach to characterizing soundscapes. Here, we illustrate this complexity using data from a national-scale effort, the Sanctuary Soundscape Monitoring Project (SanctSound), an initiative designed to support collection of biological, environmental, and human use data to compliment the interpretation of sound level measurements. Using nine examples from this diverse dataset we demonstrate the benefit of integrating source identification and site features to interpret sound levels across a diversity of shallow water marine soundscapes (<150 m). Sound levels from sites in high human use areas reflect the anthropogenic influences on the soundscape, especially when measuring broad frequency bands, whereas sites with relatively low human activity and high sound levels reveal biological features of the soundscape. At sites with large tidal changes, sound levels correlated with the magnitude of tidal flow, and sound levels during high tidal flow periods were similar to sound levels at sites near commercial shipping lanes. At sites in relatively close proximity (<30 km), sound levels diverge because of the presence of different proximate sound contributors and propagation features of the site. A review of emerging methodologies for integrated soundscape analysis, including acoustic scenes, provides a framework for interpreting soundscapes across a variety of conditions. With a global growth in monitoring efforts collecting standardized measurements over widely distributed arrays, more integrated methods are needed to advance the utility of soundscapes in marine resource management.

Community Science for Coastal Acidification Monitoring and Research - Parker Randall Gassett; Katie O’Brien-Clayton; Carolina Bastidas; Jennie E. Rheuban; Christopher, W. Hunt; Elizabeth Turner; Matthew Liebman; Emily Silva; Adam, R. Pimenta; Jason. Grear; Jackie Motyka; Daniel McCorkle; et al. (Coastal Management, 2021)

An early warning system for wave-driven coastal flooding at Imperial Beach, CA - Mark A. Merrifield; Mele Johnson; R. T. Guza; Julia W. Fiedler; Adam P. Young; Cassandra S. Henderson; Athina M. Z. Lange; William C. O’Reilly; Bonnie C. Ludka; Michele Okihiro; Timu Gallien; Kyle Pappas; et al. (Natural Hazards, 2021)
Waves overtop berms and seawalls along the shoreline of Imperial Beach (IB), CA when energetic winter swell and high tide coincide. These intermittent, few-hour long events flood low-lying areas and pose a growing inundation risk as sea levels rise. To support city flood response and management, an IB flood warning system was developed. Total water level (TWL) forecasts combine predictions of tides and sea-level anomalies with wave runup estimates based on incident wave forecasts and the nonlinear wave model SWASH. In contrast to widely used empirical runup formulas that rely on significant wave height and peak period, and use only a foreshore slope for bathymetry, the SWASH model incorporates spectral incident wave forcing and uses the cross-shore depth profile. TWL forecasts using a SWASH emulator demonstrate skill several days in advance. Observations set TWL thresholds for minor and moderate flooding. The specific wave and water level conditions that lead to flooding, and key contributors to TWL uncertainty, are identified. TWL forecast skill is reduced by errors in the incident wave forecast and the one-dimensional runup model, and lack of information of variable beach morphology (e.g., protective sand berms can erode during storms). Model errors are largest for the most extreme events. Without mitigation, projected sea-level rise will substantially increase the duration and severity of street flooding. Application of the warning system approach to other locations requires incident wave hindcasts and forecasts, numerical simulation of the runup associated with local storms and beach morphology, and model calibration with flood observations.

The effects of climate and demographic history in shaping genomic variation across populations of the Desert Horned Lizard ( Phrynosoma platyrhinos ) - Keaka Farleigh; Sarah A. Vladimirova; Christopher Blair; Jason T. Bracken; Nazila Koochekian; Drew R. Schield; Daren C. Card; Nicholas Finger; Jonathan Henault; Adam D. Leaché; Todd A. Castoe; Tereza Jezkova (Molecular Ecology, 2021)
Species often experience spatial environmental heterogeneity across their range, and populations may exhibit signatures of adaptation to local environmental characteristics. Other population genetic processes, such as migration and genetic drift, can impede the effects of local adaptation. Genetic drift in particular can have a pronounced effect on population genetic structure during large‐scale geographic expansions, where a series of founder effects leads to decreases in genetic variation in the direction of the expansion. Here, we explore the genetic diversity of a desert lizard that occupies a wide range of environmental conditions and that has experienced post‐glacial expansion northwards along two colonization routes. Based on our analyses of a large SNP data set, we find evidence that both climate and demographic history have shaped the genetic structure of populations. Pronounced genetic differentiation was evident between populations occupying cold versus hot deserts, and we detected numerous loci with significant associations with climate. The genetic signal of founder effects, however, is still present in the genomes of the recently expanded populations, which comprise subsets of genetic variation found in the southern populations.

Continental‐scale acoustic telemetry and network analysis reveal new insights into stock structure - Elodie J. I. Lédée; Michelle R. Heupel; Matthew D. Taylor; Robert G. Harcourt; Fabrice R. A. Jaine; Charlie Huveneers; Vinay Udyawer; Hamish A. Campbell; Russell C. Babcock; Xavier Hoenner; Adam Barnett; Matias Braccini; et al. (Fish and Fisheries, 2021)
Delineation of population structure (i.e. stocks) is crucial to successfully manage exploited species and to address conservation concerns for threatened species. Fish migration and associated movements are key mechanisms through which discrete populations mix and are thus important determinants of population structure. Detailed information on fish migration and movements is becoming more accessible through advances in telemetry and analysis methods however such information is not yet used systematically in stock structure assessment. Here, we described how detections of acoustically tagged fish across a continental‐scale array of underwater acoustic receivers were used to assess stock structure and connectivity in seven teleost and seven shark species and compared to findings from genetic and conventional tagging. Network analysis revealed previously unknown population connections in some species, and in others bolstered support for existing stock discrimination by identifying nodes and routes important for connectivity. Species with less variability in their movements required smaller sample sizes (45–50 individuals) to reveal useful stock structure information. Our study shows the power of continental‐scale acoustic telemetry networks to detect movements among fishery jurisdictions. We highlight methodological issues that need to be considered in the design of acoustic telemetry studies for investigating stock structure and the interpretation of the resulting data. The advent of broad‐scale acoustic telemetry networks across the globe provides new avenues to understand how movement informs population structure and can lead to improved management.

The dimensionality and structure of species trait spaces - David Mouillot; Nicolas Loiseau; Matthias Grenié; Adam C. Algar; Michele Allegra; Marc W. Cadotte; Nicolas Casajus; Pierre Denelle; Maya Guéguen; Anthony Maire; Brian Maitner; Brian J. McGill; et al. (Ecology Letters, 2021)
Trait‐based ecology aims to understand the processes that generate the overarching diversity of organismal traits and their influence on ecosystem functioning. Achieving this goal requires simplifying this complexity in synthetic axes defining a trait space and to cluster species based on their traits while identifying those with unique combinations of traits. However, so far, we know little about the dimensionality, the robustness to trait omission and the structure of these trait spaces. Here, we propose a unified framework and a synthesis across 30 trait datasets representing a broad variety of taxa, ecosystems and spatial scales to show that a common trade‐off between trait space quality and operationality appears between three and six dimensions. The robustness to trait omission is generally low but highly variable among datasets. We also highlight invariant scaling relationships, whatever organismal complexity, between the number of clusters, the number of species in the dominant cluster and the number of unique species with total species richness. When species richness increases, the number of unique species saturates, whereas species tend to disproportionately pack in the richest cluster. Based on these results, we propose some rules of thumb to build species trait spaces and estimate subsequent functional diversity indices.

Consensus Guidelines for Advancing Coral Holobiont Genome and Specimen Voucher Deposition - Christian R. Voolstra; Kate M. Quigley; Sarah W. Davies; John Everett Parkinson; Raquel S. Peixoto; Manuel Aranda; Andrew C. Baker; Adam R. Barno; Daniel J. Barshis; Francesca Benzoni; Victor Bonito; David G. Bourne; et al. (Frontiers in Marine Science, 2021)
Coral research is being ushered into the genomic era. To fully capitalize on the potential discoveries from this genomic revolution, the rapidly increasing number of high-quality genomes requires effective pairing with rigorous taxonomic characterizations of specimens and the contextualization of their ecological relevance. However, to date there is no formal framework that genomicists, taxonomists, and coral scientists can collectively use to systematically acquire and link these data. Spurred by the recently announced “Coral symbiosis sensitivity to environmental change hub” under the “Aquatic Symbiosis Genomics Project” - a collaboration between the Wellcome Sanger Institute and the Gordon and Betty Moore Foundation to generate gold-standard genome sequences for coral animal hosts and their associated Symbiodiniaceae microalgae (among the sequencing of many other symbiotic aquatic species) - we outline consensus guidelines to reconcile different types of data. The metaorganism nature of the coral holobiont provides a particular challenge in this context and is a key factor to consider for developing a framework to consolidate genomic, taxonomic, and ecological (meta)data. Ideally, genomic data should be accompanied by taxonomic references, i.e., skeletal vouchers as formal morphological references for corals and strain specimens in the case of microalgal and bacterial symbionts (cultured isolates). However, exhaustive taxonomic characterization of all coral holobiont member species is currently not feasible simply because we do not have a comprehensive understanding of all the organisms that constitute the coral holobiont. Nevertheless, guidelines on minimal, recommended, and ideal-case descriptions for the major coral holobiont constituents (coral animal, Symbiodiniaceae microalgae, and prokaryotes) will undoubtedly help in future referencing and will facilitate comparative studies. We hope that the guidelines outlined here, which we will adhere to as part of the Aquatic Symbiosis Genomics Project sub-hub focused on coral symbioses, will be useful to a broader community and their implementation will facilitate cross- and meta-data comparisons and analyses.

Exposure to degraded coral habitat depresses oxygen uptake rate during exercise of a juvenile reef fish - Adam T. Downie; Caroline M. Phelps; Rhondda Jones; Jodie L. Rummer; Douglas P. Chivers; Maud C. O. Ferrari; Mark I. McCormick (Coral Reefs, 2021)

Ancient geological dynamics impact neutral biodiversity accumulation and are detectable in phylogenetic reconstructions - Leonel Herrera‐Alsina; Adam C. Algar; Greta Bocedi; Cecile Gubry‐Rangin; Lesley T. Lancaster; Poppy Mynard; Owen G. Osborne; Alexander S. T. Papadopulos; Simon Creer; Meis Nangoy; Fahri Fahri; Pungki Lupiyaningdyah; et al. (Global Ecology and Biogeography, 2021)
Aim Landmasses have been continuously modified by tectonic activity, the breakup and collision of landmasses is thought to have generated or suppressed ecological opportunities, altering the rates of speciation, dispersal and extinction. However, the extent to which the signatures of past geological events are retained in modern biodiversity patterns—or obliterated by recent ecological dynamics—remains unresolved. We aim to identify the fingerprint of different scenarios of geological activity on phylogenetic trees and geographic range size distributions. Location Global. Time period Geological time. Major taxa studied Theoretical predictions for any taxa. Methods We conducted spatially explicit simulations under a neutral model of range evolution, speciation and extinction for three different geological scenarios that differed in their geological histories. We set a limit on the number of populations that locally can coexist, which, along with the geographic boundaries of landmasses, influences the rate of range expansion. Results Our results demonstrate regions of similar size, age and ecological limits will differ in richness and macroevolutionary patterns based solely on the geological history of landmass breakup and collision even in the absence of species’ ecological differences, that is, neutrality. When landmasses collide, regional richness is higher, lineages exhibit more similar rates of speciation and phylogenetic trees are more balanced than in the geologically static scenario. Stringent local limits to coexistence yield lower regional diversity but in general do not affect our ability to distinguish geological scenarios. Main conclusions These findings provide an alternative explanation for existence of some hotspots of diversity in areas of high geological activity. Although a limit on the number of coexisting species largely influences regional diversity, its contribution to phylogenetic patterns is lower than variation in per‐capita rates of speciation and extirpation. Importantly, these findings demonstrate the potential for inferring past geological history from distributions of phylogenies and range sizes.

Low‐intensity frequent fires in coniferous forests transform soil organic matter in ways that may offset ecosystem carbon losses - Adam F. A. Pellegrini; Anthony C. Caprio; Katerina Georgiou; Colin Finnegan; Sarah E. Hobbie; Jeffery A. Hatten; Robert B. Jackson (Global Change Biology, 2021)
The impact of shifting disturbance regimes on soil carbon (C) storage is a key uncertainty in global change research. Wildfires in coniferous forests are becoming more frequent in many regions, potentially causing large C emissions. Repeated low‐intensity prescribed fires can mitigate wildfire severity, but repeated combustion may decrease soil C unless compensatory responses stabilize soil organic matter. Here, we tested how 30 years of decadal prescribed burning affected C and nitrogen (N) in plants, detritus, and soils in coniferous forests in the Sierra Nevada mountains, USA. Tree basal area and litter stocks were resilient to fire, but fire reduced forest floor C by 77% (−36.4 Mg C/ha). In mineral soils, fire reduced C that was free from minerals by 41% (−4.4 Mg C/ha) but not C associated with minerals, and only in depths ≤ 5 cm. Fire also transformed the properties of remaining mineral soil organic matter by increasing the proportion of C in a pyrogenic form (from 3.2% to 7.5%) and associated with minerals (from 46% to 58%), suggesting the remaining soil C is more resistant to decomposition. Laboratory assays illustrated that fire reduced microbial CO 2 respiration rates by 55% and the activity of eight extracellular enzymes that degrade cellulosic and aromatic compounds by 40–66%. Lower decomposition was correlated with lower inorganic N (−49%), especially ammonium, suggesting N availability is coupled with decomposition. The relative increase in forms of soil organic matter that are resistant to decay or stabilized onto mineral surfaces, and the associated decline in decomposition suggest that low‐intensity fires may promote mineral soil C storage in pools with long mean residence times in coniferous forests.

A guide to using the internet to monitor and quantify the wildlife trade - Oliver C. Stringham; Adam Toomes; Aurelie M. Kanishka; Lewis Mitchell; Sarah Heinrich; Joshua V. Ross; Phillip Cassey (Conservation Biology, 2021)
The unrivaled growth in e‐commerce of animals and plants presents an unprecedented opportunity to monitor wildlife trade to inform conservation, biosecurity, and law enforcement. Using the internet to quantify the scale of the wildlife trade (volume and frequency) is a relatively recent and rapidly developing approach that lacks an accessible framework for locating relevant websites and collecting data. We produced an accessible guide for internet‐based wildlife trade surveillance. We detailed a repeatable method involving a systematic internet search, with search engines, to locate relevant websites and content. For data collection, we highlight web‐scraping technology as an efficient way to collect data in an automated fashion at regularly timed intervals. Our guide is applicable to the multitude of trade‐based contexts because researchers can tailor search keywords for specific taxa or derived products and locations of interest. We provide information for working with the diversity of websites used in wildlife trade. For example, to locate relevant content on social media (e.g., posts or groups), each social media platform should be examined individually via the site's internal search engine. A key advantage of using the internet to study wildlife trade is the relative ease of access to an increasing amount of trade‐related data. However, not all wildlife trade occurs online and it may occur on unobservable sections of the internet.

Evaluating the evidence for ecological effectiveness of South Africa’s marine protected areas - SP Kirkman; BQ Mann; KJ Sink; R Adams; T-C Livingstone; JB Mann-Lang; MC Pfaff; T Samaai; MG van der Bank; L Williams; GM Branch (African Journal of Marine Science, 2021)

Interpretation and management of genetic test results by Canadian family physicians: a multiple choice survey of performance - Stephanie J. Skinner; Adam T. Clay; Michelle C. E. McCarron; Sarah Liskowich (Journal of Community Genetics, 2021)

Isotopic analyses of prehistoric human remains from the Flinders Group, Queensland, Australia, support an association between burial practices and status - Shaun Adams; Michael C. Westaway; David McGahan; Doug Williams; Jian-Xin Zhao; Yuexing Feng; Ai Nguyen; John Pearce; Clarence Flinders; Mark Collard (Archaeological and Anthropological Sciences, 2021)

Tropicalization of temperate ecosystems in North America: The northward range expansion of tropical organisms in response to warming winter temperatures - Michael J. Osland; Philip W. Stevens; Margaret M. Lamont; Richard C. Brusca; Kristen M. Hart; J. Hardin Waddle; Catherine A. Langtimm; Caroline M. Williams; Barry D. Keim; Adam J. Terando; Eric A. Reyier; Katie E. Marshall; et al. (Global Change Biology, 2021)
Tropicalization is a term used to describe the transformation of temperate ecosystems by poleward‐moving tropical organisms in response to warming temperatures. In North America, decreases in the frequency and intensity of extreme winter cold events are expected to allow the poleward range expansion of many cold‐sensitive tropical organisms, sometimes at the expense of temperate organisms. Although ecologists have long noted the critical ecological role of winter cold temperature extremes in tropical–temperate transition zones, the ecological effects of extreme cold events have been understudied, and the influence of warming winter temperatures has too often been left out of climate change vulnerability assessments. Here, we examine the influence of extreme cold events on the northward range limits of a diverse group of tropical organisms, including terrestrial plants, coastal wetland plants, coastal fishes, sea turtles, terrestrial reptiles, amphibians, manatees, and insects. For these organisms, extreme cold events can lead to major physiological damage or landscape‐scale mass mortality. Conversely, the absence of extreme cold events can foster population growth, range expansion, and ecological regime shifts. We discuss the effects of warming winters on species and ecosystems in tropical–temperate transition zones. In the 21st century, climate change‐induced decreases in the frequency and intensity of extreme cold events are expected to facilitate the poleward range expansion of many tropical species. Our review highlights critical knowledge gaps for advancing understanding of the ecological implications of the tropicalization of temperate ecosystems in North America.

Evidence of historical isolation and genetic structuring among broadnose sevengill sharks (Notorynchus cepedianus) from the world’s major oceanic regions - Alicia C. J. Schmidt-Roach; Christine C. Bruels; Adam Barnett; Adam D. Miller; Craig D. H. Sherman; David A. Ebert; Sebastian Schmidt-Roach; Charlene da Silva; Christopher G. Wilke; Craig Thorburn; Jeffrey C. Mangel; Juan Manuel Ezcurra; et al. (Reviews in Fish Biology and Fisheries, 2021)

Beta-guanidinopropionic acid has age-specific effects on markers of health and function in mice - Jonathan D. Dorigatti; Kevin M. Thyne; Brett C. Ginsburg; Adam B. Salmon (GeroScience, 2021)

Is impaired dopaminergic function associated with mobility capacity in older adults? - Simon Moskowitz; David W. Russ; Leatha A. Clark; Nathan P. Wages; Dustin R. Grooms; Adam J. Woods; Julie Suhr; Janet E. Simon; Andrew O’Shea; Cody R. Criss; Paolo Fadda; Brian C. Clark (GeroScience, 2021)

Public Preferences for Longleaf Pine Restoration Programs in the Southeastern United States - Sydney Oluoch; Pankaj Lal; Bernabas Wolde; Andres Susaeta; Josè R Soto; Meghann Smith; Damian C Adams (Forest Science, 2021)
Longleaf pine (LLP) (Pinus palustris Mill.) is well known for its role in supporting healthy ecosystems in the southeastern (SE) United States (US). The decline of LLP forest ecosystems has led to a consensus among stakeholders that restoration efforts are needed. However, there is still a lack of robust understanding of the utilization of nonmarket ecosystem services of LLP forests. These challenges have presented major barriers to landowner acceptance of subsidized LLP restoration programs. Understanding the tradeoffs between forest ecosystem services is critical to restoring LLP in the SE US. This study employs the best-worst choice (BWC) method to assess public preferences toward hypothetical LLP restoration programs that consider ecosystem services such as recreation, timber production, carbon sequestration, water yield, and wildlife diversity. We surveyed a representative sample of n = 953 respondents from Alabama, Mississippi, Georgia, and Florida, and results showed that residents in all four states are willing to pay for LLP restoration, with the highest average willingness to pay (WTP) for forest recreation ($20.39), followed by red-cockaded woodpecker (RCW) conservation ($13.37) and carbon sequestration ($13.32). This research provides important public preference information on ecosystem services that is critical in forming sustainable LLP restoration programs.

The Influence of Leaf Type on Carbon and Nitrogen Assimilation by Aquatic Invertebrate Communities: A New Perspective on Trophic Efficiency - Adam C. Siders; Zacchaeus G. Compson; Bruce A. Hungate; Paul Dijkstra; George W. Koch; Jane C. Marks (Ecosystems, 2021)

A Simplified In Silico Model of Left Ventricular Outflow in Patients After Transcatheter Mitral Valve Replacement with Anterior Leaflet Laceration - Keshav Kohli; Zhenglun Alan Wei; Vahid Sadri; Tiffany Netto; John C. Lisko; Adam B. Greenbaum; Vasilis Babaliaros; John N. Oshinski; Ajit P. Yoganathan (Annals of Biomedical Engineering, 2021)

Mangrove growth response to experimental warming is greatest near the range limit in northeast Florida - Samantha K. Chapman; Ilka C. Feller; Gabriela Canas; Matthew A. Hayes; Nicole Dix; Mark Hester; Jim Morris; J. Adam Langley (Ecology, 2021)
Shrubs are invading into grasslands around the world, but we don’t yet know how these shrubs will fare in a warmer future. In ecotonal coastal wetland ecosystems, woody mangroves are encroaching into herbaceous salt marshes owing to changes in temperature, precipitation, and sediment dynamics. Increasing mangrove biomass in wetlands often increases carbon storage, which is high in these productive ecosystems, but little is known about how mangrove growth will change in response to warming. To address this knowledge gap, we deployed warming experiments at three coastal wetland sites along a latitudinal gradient in northeast Florida where Avicennia germinans , black mangroves, are encroaching into salt marshes. We achieved air temperature warming (+1.6°C during the day) at all three sites and measured stem elongation, canopy height and area changes, and leaf and node number. After 2 yr of warming, we found that mangrove growth rate in height increased due to warming. Warming increased stem elongation by 130% over unwarmed control plots after 1 yr at the northern site. Mangrove growth in canopy area did not respond to warming. Site differences in growth rate were pronounced, and mangrove growth in both height and area were lowest at the northern site, despite greater impacts of warming at that site. We also found that area‐based relative growth rate was five times higher across all treatments than height‐based relative growth rate, indicating that mangroves are growing wider rather than taller in these ecotonal environments. Our findings indicate that the growth effect of experimental warming depends on site characteristics and growth parameter measured. We also propose that differential mangrove growth across the three sites may be driven by biotic factors such as the identity of the salt marsh species into which mangroves are encroaching. Our results suggest that, as seen in other ecosystems, wetland plants may respond most strongly to warming at their poleward range edge.

SNAPSHOT USA 2019: a coordinated national camera trap survey of the United States - Michael V. Cove; Roland Kays; Helen Bontrager; Claire Bresnan; Monica Lasky; Taylor Frerichs; Renee Klann; Thomas E. Lee; Seth C. Crockett; Anthony P. Crupi; Katherine C. B. Weiss; Helen Rowe; et al. (Ecology, 2021)
With the accelerating pace of global change, it is imperative that we obtain rapid inventories of the status and distribution of wildlife for ecological inferences and conservation planning. To address this challenge, we launched the SNAPSHOT USA project, a collaborative survey of terrestrial wildlife populations using camera traps across the United States. For our first annual survey, we compiled data across all 50 states during a 14‐week period (17 August–24 November of 2019). We sampled wildlife at 1,509 camera trap sites from 110 camera trap arrays covering 12 different ecoregions across four development zones. This effort resulted in 166,036 unique detections of 83 species of mammals and 17 species of birds. All images were processed through the Smithsonian’s eMammal camera trap data repository and included an expert review phase to ensure taxonomic accuracy of data, resulting in each picture being reviewed at least twice. The results represent a timely and standardized camera trap survey of the United States. All of the 2019 survey data are made available herein. We are currently repeating surveys in fall 2020, opening up the opportunity to other institutions and cooperators to expand coverage of all the urban–wild gradients and ecophysiographic regions of the country. Future data will be available as the database is updated at eMammal.si.edu/snapshot‐usa, as will future data paper submissions. These data will be useful for local and macroecological research including the examination of community assembly, effects of environmental and anthropogenic landscape variables, effects of fragmentation and extinction debt dynamics, as well as species‐specific population dynamics and conservation action plans. There are no copyright restrictions; please cite this paper when using the data for publication.

Resource Occurrence and Productivity in Existing and Proposed Wind Energy Lease Areas on the Northeast US Shelf - Kevin D. Friedland; Elizabeth T. Methratta; Andrew B. Gill; Sarah K. Gaichas; Tobey H. Curtis; Evan M. Adams; Janelle L. Morano; Daniel P. Crear; M. Conor McManus; Damian C. Brady (Frontiers in Marine Science, 2021)
States in the Northeast United States have the ambitious goal of producing more than 22 GW of offshore wind energy in the coming decades. The infrastructure associated with offshore wind energy development is expected to modify marine habitats and potentially alter the ecosystem services. Species distribution models were constructed for a group of fish and macroinvertebrate taxa resident in the Northeast US Continental Shelf marine ecosystem. These models were analyzed to provide baseline context for impact assessment of lease areas in the Middle Atlantic Bight designated for renewable wind energy installations. Using random forest machine learning, models based on occurrence and biomass were constructed for 93 species providing seasonal depictions of their habitat distributions. We developed a scoring index to characterize lease area habitat use for each species. Subsequently, groups of species were identified that reflect varying levels of lease area habitat use ranging across high, moderate, low, and no reliance on the lease area habitats. Among the species with high to moderate reliance were black sea bass ( Centropristis striata ), summer flounder ( Paralichthys dentatus ), and Atlantic menhaden ( Brevoortia tyrannus ), which are important fisheries species in the region. Potential for impact was characterized by the number of species with habitat dependencies associated with lease areas and these varied with a number of continuous gradients. Habitats that support high biomass were distributed more to the northeast, while high occupancy habitats appeared to be further from the coast. There was no obvious effect of the size of the lease area on the importance of associated habitats. Model results indicated that physical drivers and lower trophic level indicators might strongly control the habitat distribution of ecologically and commercially important species in the wind lease areas. Therefore, physical and biological oceanography on the continental shelf proximate to wind energy infrastructure development should be monitored for changes in water column structure and the productivity of phytoplankton and zooplankton and the effects of these changes on the trophic system.

Detrital zircon age studies of Haast Schist in western Otago and Marlborough, New Zealand: constraints on their protolith age, terrane ancestry and Au–W mineralisation - C. J. Adams; H. J. Campbell; W. L. Griffin (Australian Journal of Earth Sciences, 2021)

Nitrogen limitation and calcifuge plant strategies constrain the establishment of native vegetation on magnetite mine tailings - Adam T. Cross; Dmitry Ivanov; Jason C. Stevens; Rohan Sadler; Hongtao Zhong; Hans Lambers; Kingsley W. Dixon (Plant and Soil, 2021)

Compromised root development constrains the establishment potential of native plants in unamended alkaline post-mining substrates - Adam T. Cross; Jason C. Stevens; Rohan Sadler; Benjamin Moreira-Grez; Dmitry Ivanov; Hongtao Zhong; Kingsley W. Dixon; Hans Lambers (Plant and Soil, 2021)

Locally abundant, endangered Mariana swiftlets impact the abundance, behavior, and body condition of an invasive predator - Page E. Klug; Amy A. Yackel Adams; Shane R. Siers; Kevin M. Brindock; Stephen M. Mosher; M. J. Mazurek; William C. Pitt; Robert N. Reed (Oecologia, 2021)

Not all methods are created equal: assessment of sampling methods for crayfishes and fishes in southern Appalachian streams - Zanethia C. Barnett; Clifford A. Ochs; Jason D. Hoeksema; Susan B. Adams (Hydrobiologia, 2021)

Mapping the multicausality of Alzheimer’s disease through group model building - Jeroen F. Uleman; René J. F. Melis; Rick Quax; Eddy A. van der Zee; Dick Thijssen; Martin Dresler; Ondine van de Rest; Isabelle F. van der Velpen; Hieab H. H. Adams; Ben Schmand; Inge M. C. M. de Kok; Jeroen de Bresser; et al. (GeroScience, 2021)
Alzheimer’s disease (AD) is a complex, multicausal disorder involving several spatiotemporal scales and scientific domains. While many studies focus on specific parts of this system, the complexity of AD is rarely studied as a whole. In this work, we apply systems thinking to map out known causal mechanisms and risk factors ranging from intracellular to psychosocial scales in sporadic AD. We report on the first systemic causal loop diagram (CLD) for AD, which is the result of an interdisciplinary group model building (GMB) process. The GMB was based on the input of experts from multiple domains and all proposed mechanisms were supported by scientific literature. The CLD elucidates interaction and feedback mechanisms that contribute to cognitive decline from midlife onward as described by the experts. As an immediate outcome, we observed several non-trivial reinforcing feedback loops involving factors at multiple spatial scales, which are rarely considered within the same theoretical framework. We also observed high centrality for modifiable risk factors such as social relationships and physical activity, which suggests they may be promising leverage points for interventions. This illustrates how a CLD from an interdisciplinary GMB process may lead to novel insights into complex disorders. Furthermore, the CLD is the first step in the development of a computational model for simulating the effects of risk factors on AD.

Correction to: Beyond one-way determinism: San Frediano’s miracle and climate change in Central and Northern Italy in late antiquity - Giovanni Zanchetta; Monica Bini; Kevin Bloomfield; Adam Izdebski; Nicola Vivoli; Eleonora Regattieri; Ilaria Isola; Russell N. Drysdale; Petra Bajo; John C. Hellstrom; Robert Wiśniewski; Anthony E. Fallick; et al. (Climatic Change, 2021)

Coordinating Global Multi-Site Studies of Military-Relevant Traumatic Brain Injury: Opportunities, Challenges, and Harmonization Guidelines - David F Tate; Emily L Dennis; John T Adams; Maheen M Adamson; Heather G Belanger; Erin D Bigler; Heather C Bouchard; Alexandra L Clark; Lisa M Delano-Wood; Seth G Disner; Blessen C Eapen; Carol E Franz; et al. (Brain Imaging and Behavior, 2021)

An fMRI study of age-associated changes in basic visual discrimination - Talia R. Seider; Eric C. Porges; Adam J. Woods; Ronald A. Cohen (Brain Imaging and Behavior, 2021)

Effects of cardenolides of milkweed plants on immunity of the monarch butterfly - Kandis L. Adams; Ahmed Aljohani; Joselyne Chavez; Jacobus C. de Roode (Arthropod-Plant Interactions, 2021)

Abundance and biogeography of methanogenic and methanotrophic microorganisms across European streams - Magdalena Nagler; Nadine Praeg; Georg H. Niedrist; Katrin Attermeyer; Núria Catalán; Francesca Pilotto; Catherine Gutmann Roberts; Christoph Bors; Stefano Fenoglio; Miriam Colls; Sophie Cauvy‐Fraunié; Brian Doyle; et al. (Journal of Biogeography, 2021)
Aim Although running waters are getting recognized as important methane sources, large‐scale geographical patterns of microorganisms controlling the net methane balance of streams are still unknown. Here we aim at describing community compositions of methanogenic and methanotrophic microorganisms at large spatial scales and at linking their abundances to potential sediment methane production (PMP) and oxidation rates (PMO). Location The study spans across 16 European streams from northern Spain to northern Sweden and from western Ireland to western Bulgaria. Taxon Methanogenic archaea and methane‐oxidizing microorganisms. Methods To provide a geographical overview of both groups in a single approach, microbial communities and abundances were investigated via 16S rRNA gene sequencing, extracting relevant OTUs based on literature; both groups were quantified via quantitative PCR targeting mcrA and pmoA genes and studied in relation to environmental parameters, sediment PMP and PMO, and land use. Results Diversity of methanogenic archaea was higher in warmer streams and of methanotrophic communities in southern sampling sites and in larger streams. Anthropogenically altered, warm and oxygen‐poor streams were dominated by the highly efficient methanogenic families Methanospirillaceae , Methanosarcinaceae and Methanobacteriaceae , but did not harbour any specific methanotrophic organisms. Contrastingly, sediment communities in colder, oxygen‐rich waters with little anthropogenic impact were characterized by methanogenic Methanosaetaceae , Methanocellaceae and Methanoflorentaceae and methanotrophic Methylococcaceae and Cd . Methanoperedens. Representatives of the methanotrophic Crenotrichaceae and Methylococcaceae as well as the methanogenic Methanoregulaceae were characteristic for environments with larger catchment area and higher discharge. PMP increased with increasing abundance of methanogenic archaea, while PMO rates did not show correlations with abundances of methane‐oxidizing bacteria. Main conclusions Methanogenic and methanotrophic communities grouping into three habitat types suggest that future climate‐ and land use changes may influence the prevailing microbes involved in the large‐scale stream‐related methane cycle, favouring the growth of highly efficient hydrogenotrophic methane producers. Based on these results, we expect global change effect on PMP rates to especially impact rivers adjacent to anthropogenically disturbed land uses.

Flexible Manufacturing: The Future State of Drug Product Development and Commercialization in the Pharmaceutical Industry - Yash Kapoor; Robert F. Meyer; Brian K. Meyer; James C. DiNunzio; Akhilesh Bhambhani; Justin Stanbro; Kristin J. M. Ploeger; Erin N. Guidry; Gregory M. Troup; Adam T. Procopio; Allen C. Templeton (Journal of Pharmaceutical Innovation, 2021)

Beyond one-way determinism: San Frediano’s miracle and climate change in Central and Northern Italy in late antiquity - Giovanni Zanchetta; Monica Bini; Kevin Bloomfield; Adam Izdebski; Nicola Vivoli; Eleonora Regattieri; Ilaria Isola; Russell N. Drysdale; Petra Bajo; John C. Hellstrom; Robert Wiśniewski; Anthony E. Fallick; et al. (Climatic Change, 2021)
Integrating palaeoclimatological proxies and historical records, which is necessary to achieve a more complete understanding of climate impacts on past societies, is a challenging task, often leading to unsatisfactory and even contradictory conclusions. This has until recently been the case for Italy, the heart of the Roman Empire, during the transition between Antiquity and the Middle Ages. In this paper, we present new high-resolution speleothem data from the Apuan Alps (Central Italy). The data document a period of very wet conditions in the sixth c. AD, probably related to synoptic atmospheric conditions similar to a negative phase of the North Atlantic Oscillation. For this century, there also exist a significant number of historical records of extreme hydroclimatic events, previously discarded as anecdotal. We show that this varied evidence reflects the increased frequency of floods and extreme rainfall events in Central and Northern Italy at the time. Moreover, we also show that these unusual hydroclimatic conditions overlapped with the increased presence of “water miracles” in Italian hagiographical accounts and social imagination. The miracles, performed by local Church leaders, strengthened the already growing authority of holy bishops and monks in Italian society during the crucial centuries that followed the “Fall of the Roman Empire”. Thus, the combination of natural and historical data allows us to show the degree to which the impact of climate variability on historical societies is determined not by the nature of the climatic phenomena per se, but by the culture and the structure of the society that experienced it.

Signatures of selection in a recent invasion reveal adaptive divergence in a highly vagile invasive species - Katarina C. Stuart; Adam P. A. Cardilini; Phillip Cassey; Mark F. Richardson; William B. Sherwin; Lee A. Rollins; Craig D. H. Sherman (Molecular Ecology, 2021)
A detailed understanding of population genetics in invasive populations helps us to identify drivers of successful alien introductions. Here, we investigate putative signals of selection in Australian populations of invasive common starlings, Sturnus vulgaris , and seek to understand how these have been influenced by introduction history. We used reduced representation sequencing to determine population structure, and identify Single Nucleotide Polymorphisms (SNPs) that are putatively under selection. We found that since their introduction into Australia, starling populations have become genetically differentiated despite the potential for high levels of dispersal, and that starlings have responded to selective pressures imposed by a wide range of environmental conditions across their geographic range. Isolation by distance appears to have played a strong role in determining genetic substructure across the starling's Australian range. Analyses of candidate SNPs that are putatively under selection indicated that aridity, precipitation and temperature may be important factors driving adaptive variation across the starling's invasive range in Australia. However, we also noted that the historic introduction regime may leave footprints on sites flagged as being under adaptive selection, and encourage critical interpretation of selection analyses in non‐native populations.

The Accelerations of a Wave Measurement Buoy Impacted by Breaking Waves in the Surf Zone - Adam C. Brown; Robert K. Paasch (Journal of Marine Science and Engineering, 2021)
A spherical wave measurement buoy capable of detecting breaking waves has been designed and built. The buoy is 16 inches in diameter and houses a 9 degree of freedom inertial measurement unit (IMU). The orientation and acceleration of the buoy is continuously logged at frequencies up to 200 Hz providing a high fidelity description of the motion of the buoy as it is impacted by breaking waves. The buoy was deployed several times throughout the winter of 2013–2014. Both moored and free-drifting data were acquired in near-shore shoaling waves off the coast of Newport, OR. Almost 200 breaking waves of varying type and intensity were measured over the course of multiple deployments. The characteristic signature of spilling and plunging breakers was identified in the IMU data.

Monitoring wetland water quality related to livestock grazing in amphibian habitats - Kelly L. Smalling; Jennifer C. Rowe; Christopher A. Pearl; Luke R. Iwanowicz; Carrie E. Givens; Chauncey W. Anderson; Brome McCreary; Michael J. Adams (Environmental Monitoring and Assessment, 2021)
Land use alteration such as livestock grazing can affect water quality in habitats of at-risk wildlife species. Data from managed wetlands are needed to understand levels of exposure for aquatic life stages and monitor grazing-related changes afield. We quantified spatial and temporal variation in water quality in wetlands occupied by threatened Oregon spotted frog ( Rana pretiosa ) at Klamath Marsh National Wildlife Refuge in Oregon, United States (US). We used analyses for censored data to evaluate the importance of habitat type and grazing history in predicting concentrations of nutrients, turbidity, fecal indicator bacteria (FIB; total coliforms, Escherichia coli ( E. coli ), and enterococci), and estrogenicity, an indicator of estrogenic activity. Nutrients (orthophosphate and ammonia) and enterococci varied over time and space, while E. coli , total coliforms, turbidity, and estrogenicity were more strongly associated with local livestock grazing metrics. Turbidity was correlated with several grazing-related constituents and may be particularly useful for monitoring water quality in landscapes with livestock use. Concentrations of orthophosphate and estrogenicity were elevated at several sites relative to published health benchmarks, and their potential effects on Rana pretiosa warrant further investigation. Our data provided an initial assessment of potential exposure of amphibians to grazing-related constituents in western US wetlands. Increased monitoring of surface water quality and amphibian population status in combination with controlled laboratory toxicity studies could help inform future research and targeted management strategies for wetlands with both grazing and amphibians of conservation concern.

Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans ‐induced amoebic gill disease (AGD) - Chloe J. English; Natasha A. Botwright; Mark B. Adams; Andrew C. Barnes; James W. Wynne; Paula C. Lima; Mathew T. Cook (Journal of Fish Diseases, 2021)
Amoebic gill disease (AGD) is one of the main health issues impacting farmed Atlantic salmon. Neoparamoeba perurans causes AGD; however, a diversity of other amoeba species colonizes the gills and there is little understanding of whether they are commensal or potentially involved in different stages of gill disease development. Here, we conduct in vivo challenges of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. to investigate their pathogenicity to Atlantic salmon gills. Additionally, we assessed whether the presence of Nolandella sp. and Pseudoparamoeba sp. influences the onset and/or severity of N. perurans ‐induced AGD. All three strains attached and multiplied on the gills according to qPCR analysis. Furthermore, minor gross gill lesions and histological changes were observed post‐exposure. While N. perurans was found associated with classical AGD lesions, Nolandella sp. and Pseudoparamoeba sp. were not found associated with lesion sites and these lesions did not meet the expected composite of histopathological changes for AGD. Moreover, the presence of these non‐ N. perurans species did not significantly increase the severity of AGD. This trial provides evidence that cultured Nolandella sp. and Pseudoparamoeba sp. do not induce AGD and do not influence the severity of AGD during the early stages of development.

Tropical peatlands and their contribution to the global carbon cycle and climate change - Kelly Ribeiro; Felipe S. Pacheco; José W. Ferreira; Eráclito R. de Sousa‐Neto; Adam Hastie; Guenther C. Krieger Filho; Plínio C. Alvalá; Maria C. Forti; Jean P. Ometto (Global Change Biology, 2021)
Peatlands are carbon‐rich ecosystems that cover 185–423 million hectares (Mha) of the earth's surface. The majority of the world's peatlands are in temperate and boreal zones, whereas tropical ones cover only a total area of 90–170 Mha. However, there are still considerable uncertainties in C stock estimates as well as a lack of information about depth, bulk density and carbon accumulation rates. The incomplete data are notable especially in tropical peatlands located in South America, which are estimated to have the largest area of peatlands in the tropical zone. This paper displays the current state of knowledge surrounding tropical peatlands and their biophysical characteristics, distribution and carbon stock, role in the global climate, the impacts of direct human disturbances on carbon accumulation rates and greenhouse gas (GHG) emissions. Based on the new peat extension and depth data, we estimate that tropical peatlands store 152–288 Gt C, or about half of the global peatland emitted carbon. We discuss the knowledge gaps in research on distribution, depth, C stock and fluxes in these ecosystems which play an important role in the global carbon cycle and risk releasing large quantities of GHGs into the atmosphere (CO 2 and CH 4 ) when subjected to anthropogenic interferences (e.g., drainage and deforestation). Recent studies show that although climate change has an impact on the carbon fluxes of these ecosystems, the direct anthropogenic disturbance may play a greater role. The future of these systems as carbon sinks will depend on advancing current scientific knowledge and incorporating local understanding to support policies geared toward managing and conserving peatlands in vulnerable regions, such as the Amazon where recent records show increased forest fires and deforestation.

Fine-scale foraging effort and efficiency of Macaroni penguins is influenced by prey type, patch density and temporal dynamics - G. J. Sutton; C. A. Bost; A. Z. Kouzani; S. D. Adams; K. Mitchell; J. P. Y. Arnould (Marine Biology, 2021)

Techniques for In Vivo Measurement of Ligament and Tendon Strain: A Review - Qiang Zhang; Naomi C. Adam; S. H. Hosseini Nasab; William R. Taylor; Colin R. Smith (Annals of Biomedical Engineering, 2021)
The critical clinical and scientific insights achieved through knowledge of in vivo musculoskeletal soft tissue strains has motivated the development of relevant measurement techniques. This review provides a comprehensive summary of the key findings, limitations, and clinical impacts of these techniques to quantify musculoskeletal soft tissue strains during dynamic movements. Current technologies generally leverage three techniques to quantify in vivo strain patterns, including implantable strain sensors, virtual fibre elongation, and ultrasound. (1) Implantable strain sensors enable direct measurements of tissue strains with high accuracy and minimal artefact, but are highly invasive and current designs are not clinically viable. (2) The virtual fibre elongation method tracks the relative displacement of tissue attachments to measure strains in both deep and superficial tissues. However, the associated imaging techniques often require exposure to radiation, limit the activities that can be performed, and only quantify bone-to-bone tissue strains. (3) Ultrasound methods enable safe and non-invasive imaging of soft tissue deformation. However, ultrasound can only image superficial tissues, and measurements are confounded by out-of-plane tissue motion. Finally, all in vivo strain measurement methods are limited in their ability to establish the slack length of musculoskeletal soft tissue structures. Despite the many challenges and limitations of these measurement techniques, knowledge of in vivo soft tissue strain has led to improved clinical treatments for many musculoskeletal pathologies including anterior cruciate ligament reconstruction, Achilles tendon repair, and total knee replacement. This review provides a comprehensive understanding of these measurement techniques and identifies the key features of in vivo strain measurement that can facilitate innovative personalized sports medicine treatment.

The relationship between atmospheric circulation patterns and extreme temperature events in North America - Ryan E. Adams; Cameron C. Lee; Erik T. Smith; Scott C. Sheridan (International Journal of Climatology, 2021)
Extreme temperature events (ETEs) pose a significant risk to society, especially vulnerable populations with limited access to shelter and water and those with pre‐existing respiratory and cardiovascular ailments. This research examines the relationship of atmospheric circulation with a myriad of metrics related to ETEs to better understand which synoptic‐scale circulations are likely to have negative health/thermal comfort outcomes. Daily sea‐level pressure (SLP) and 500‐hPa geopotential height (z500) data from the North American Regional Reanalysis (NARR) were used to identify circulation patterns over North America. Self‐organizing maps were used to partition the variability in circulation patterns over five distinct domains covering North America for both variables. Daily 2‐m temperature, 2‐m dewpoint temperature, and 10‐m wind data from the NARR were used to derive five major categories of ETEs based on 95th percentiles: temperature events, apparent temperature events, dew point events, and excess heat and excess cold temperature events. The relationship of circulation pattern frequencies (SOM nodes) leading up to ETEs were assessed using point biserial correlations, accounting for spatial and temporal autocorrelation. The results show that z500 has a stronger association with ETEs than does SLP. A great deal of spatial variability exists in the strength of relationship for many ETE variables with circulation patterns likely due to the local geographical influence (e.g., leeside mountain adiabatic warming and low‐level maritime flow). Generally, high extremes are associated with broad ridging and anticyclonic flow and cold extremes are associated with high amplitude trough patterns with low‐level flow originating from the continental interior. The use of self‐organizing maps presents a unique way of examining the potential for human health risks related ETEs and may be an effective method for statistically downscaling climate model data to assess the potential for ETEs in the future.

Examining trends in multiple parameters of seasonally‐relative extreme temperature and dew point events across North America - Cameron C. Lee; Omon Obarein; Scott C. Sheridan; Erik T. Smith; Ryan Adams (International Journal of Climatology, 2021)
Concurrent with the background rise in global mean temperatures, changes in extreme events are also becoming evident, and are arguably more impactful on society. This research examines trends in three components of seasonally‐relative extreme temperature and humidity events in North America that directly influence human thermal comfort: event frequency, duration, and areal extent. Results indicate that for the majority of the study domain, changes in these events are in the expected direction with changes in means. Extreme heat events are generally increasing throughout the domain, with the largest changes in summer and autumn in the eastern portion of Canada and the United States. Cold events are largely decreasing in these same locations and seasons, with additional widespread decreases in winter. Interestingly, significant increases in cold events are also evident in autumn in parts of the western United States. Extreme humidity events are showing an even greater change than temperature events – nearly all of Canada and most of the United States is seeing significant increases in extreme humid events and decreases in dry events, while the southwestern deserts show widespread significant increases in dry events, especially in winter and spring. Changes in event duration and spatial extent mimic these results. Importantly, this research demonstrates that there are regions that show changes to extreme events that differ from the overall changes in means, highlighting the importance of looking beyond climate averages to examine not only extreme events, but changes in higher‐order statistical moments.

A global analysis of complexity–biodiversity relationships on marine artificial structures - Elisabeth M. A. Strain; Peter D. Steinberg; Maria Vozzo; Emma L. Johnston; Marco Abbiati; Moises A. Aguilera; Laura Airoldi; J. David Aguirre; Gail Ashton; Maritina Bernardi; Paul Brooks; Benny K. K. Chan; et al. (Global Ecology and Biogeography, 2021)
Aim Topographic complexity is widely accepted as a key driver of biodiversity, but at the patch‐scale, complexity–biodiversity relationships may vary spatially and temporally according to the environmental stressors complexity mitigates, and the species richness and identity of potential colonists. Using a manipulative experiment, we assessed spatial variation in patch‐scale effects of complexity on intertidal biodiversity. Location 27 sites within 14 estuaries/bays distributed globally. Time period 2015–2017. Major taxa studied Functional groups of algae, sessile and mobile invertebrates. Methods Concrete tiles of differing complexity (flat; 2.5‐cm or 5‐cm complex) were affixed at low–high intertidal elevation on coastal defence structures, and the richness and abundance of the colonizing taxa were quantified after 12 months. Results The patch‐scale effects of complexity varied spatially and among functional groups. Complexity had neutral to positive effects on total, invertebrate and algal taxa richness, and invertebrate abundances. However, effects on the abundance of algae ranged from positive to negative, depending on location and functional group. The tidal elevation at which tiles were placed accounted for some variation. The total and invertebrate richness were greater at low or mid than at high intertidal elevations. Latitude was also an important source of spatial variation, with the effects of complexity on total richness and mobile mollusc abundance greatest at lower latitudes, whilst the cover of sessile invertebrates and sessile molluscs responded most strongly to complexity at higher latitudes. Conclusions After 12 months, patch‐scale relationships between biodiversity and habitat complexity were not universally positive. Instead, the relationship varied among functional groups and according to local abiotic and biotic conditions. This result challenges the assumption that effects of complexity on biodiversity are universally positive. The variable effect of complexity has ramifications for community and applied ecology, including eco‐engineering and restoration that seek to bolster biodiversity through the addition of complexity.

Global root traits (GRooT) database - Nathaly R. Guerrero‐Ramírez; Liesje Mommer; Grégoire T. Freschet; Colleen M. Iversen; M. Luke McCormack; Jens Kattge; Hendrik Poorter; Fons van der Plas; Joana Bergmann; Thom W. Kuyper; Larry M. York; Helge Bruelheide; et al. (Global Ecology and Biogeography, 2021)
Motivation Trait data are fundamental to the quantitative description of plant form and function. Although root traits capture key dimensions related to plant responses to changing environmental conditions and effects on ecosystem processes, they have rarely been included in large‐scale comparative studies and global models. For instance, root traits remain absent from nearly all studies that define the global spectrum of plant form and function. Thus, to overcome conceptual and methodological roadblocks preventing a widespread integration of root trait data into large‐scale analyses we created the Global Root Trait (GRooT) Database. GRooT provides ready‐to‐use data by combining the expertise of root ecologists with data mobilization and curation. Specifically, we (a) determined a set of core root traits relevant to the description of plant form and function based on an assessment by experts, (b) maximized species coverage through data standardization within and among traits, and (c) implemented data quality checks. Main types of variables contained GRooT contains 114,222 trait records on 38 continuous root traits. Spatial location and grain Global coverage with data from arid, continental, polar, temperate and tropical biomes. Data on root traits were derived from experimental studies and field studies. Time period and grain Data were recorded between 1911 and 2019. Major taxa and level of measurement GRooT includes root trait data for which taxonomic information is available. Trait records vary in their taxonomic resolution, with subspecies or varieties being the highest and genera the lowest taxonomic resolution available. It contains information for 184 subspecies or varieties, 6,214 species, 1,967 genera and 254 families. Owing to variation in data sources, trait records in the database include both individual observations and mean values. Software format GRooT includes two csv files. A GitHub repository contains the csv files and a script in R to query the database.

High-Resolution Sampling of a Broad Marine Life Size Spectrum Reveals Differing Size- and Composition-Based Associations With Physical Oceanographic Structure - Adam T. Greer; John C. Lehrter; Benjamin M. Binder; Aditya R. Nayak; Ranjoy Barua; Ana E. Rice; Jonathan H. Cohen; Malcolm N. McFarland; Alexis Hagemeyer; Nicole D. Stockley; Kevin M. Boswell; Igor Shulman; et al. (Frontiers in Marine Science, 2020)
Observing multiple size classes of organisms, along with oceanographic properties and water mass origins, can improve our understanding of the drivers of aggregations, yet acquiring these measurements remains a fundamental challenge in biological oceanography. By deploying multiple biological sampling systems, from conventional bottle and net sampling to in situ imaging and acoustics, we describe the spatial patterns of different size classes of marine organisms (several microns to ∼10 cm) in relation to local and regional (m to km) physical oceanographic conditions on the Delaware continental shelf. The imaging and acoustic systems deployed included (in ascending order of target organism size) an imaging flow cytometer (CytoSense), a digital holographic imaging system (HOLOCAM), an In Situ Ichthyoplankton Imaging System (ISIIS, 2 cameras with different pixel resolutions), and multi-frequency acoustics (SIMRAD, 18 and 38 kHz). Spatial patterns generated by the different systems showed size-dependent aggregations and differing connections to horizontal and vertical salinity and temperature gradients that would not have been detected with traditional station-based sampling (∼9-km resolution). A direct comparison of the two ISIIS cameras showed composition and spatial patchiness changes that depended on the organism size, morphology, and camera pixel resolution. Large zooplankton near the surface, primarily composed of appendicularians and gelatinous organisms, tended to be more abundant offshore near the shelf break. This region was also associated with high phytoplankton biomass and higher overall organism abundances in the ISIIS, acoustics, and targeted net sampling. In contrast, the inshore region was dominated by hard-bodied zooplankton and had relatively low acoustic backscatter. The nets showed a community dominated by copepods, but they also showed high relative abundances of soft-bodied organisms in the offshore region where these organisms were quantified by the ISIIS. The HOLOCAM detected dense patches of ciliates that were too small to be captured in the nets or ISIIS imagery. This near-simultaneous deployment of different systems enables the description of the spatial patterns of different organism size classes, their spatial relation to potential prey and predators, and their association with specific oceanographic conditions. These datasets can also be used to evaluate the efficacy of sampling techniques, ultimately aiding in the design of efficient, hypothesis-driven sampling programs that incorporate these complementary technologies.

A synthesis of patterns of environmental mercury inputs, exposure and effects in New York State - D. C. Evers; A. K. Sauer; D. A. Burns; N. S. Fisher; D. C. Bertok; E. M. Adams; M. E. H. Burton; C. T. Driscoll (Ecotoxicology, 2020)
Mercury (Hg) pollution is an environmental problem that adversely affects human and ecosystem health at local, regional, and global scales—including within New York State. More than two-thirds of the Hg currently released to the environment originates, either directly or indirectly, from human activities. Since the early 1800s, global atmospheric Hg concentrations have increased by three- to eight-fold over natural levels. In the U.S., atmospheric emissions and point-source releases to waterways increased following industrialization into the mid-1980s. Since then, water discharges have largely been curtailed. As a result, Hg emissions, atmospheric concentrations, and deposition over the past few decades have declined across the eastern U.S. Despite these decreases, Hg pollution persists. To inform policy efforts and to advance public understanding, the New York State Energy Research and Development Authority (NYSERDA) sponsored a scientific synthesis of information on Hg in New York State. This effort includes 23 papers focused on Hg in atmospheric deposition, water, fish, and wildlife published in Ecotoxicology . New York State experiences Hg contamination largely due to atmospheric deposition. Some landscapes are inherently sensitive to Hg inputs driven by the transport of inorganic Hg to zones of methylation, the conversion of inorganic Hg to methylmercury, and the bioaccumulation and biomagnification along food webs. Mercury concentrations exceed human and ecological risk thresholds in many areas of New York State, particularly the Adirondacks, Catskills, and parts of Long Island. Mercury concentrations in some biota have declined in the Eastern Great Lakes Lowlands and the Northeastern Highlands over the last four decades, concurrent with decreases in water releases and air emissions from regional and U.S. sources. However, widespread changes have not occurred in other ecoregions of New York State. While the timing and magnitude of the response of Hg levels in biota varies, policies expected to further diminish Hg emissions should continue to decrease Hg concentrations in food webs, yielding benefits to the fish, wildlife, and people of New York State. Anticipated improvements in the Hg status of aquatic ecosystems are likely to be greatest for inland surface waters and should be roughly proportional to declines in atmospheric Hg deposition. Efforts that advance recovery from Hg pollution in recent years have yielded significant progress, but Hg remains a pollutant of concern. Indeed, due to this extensive compilation of Hg observations in biota, it appears that the extent and intensity of the contamination on the New York landscape and waterscape is greater than previously recognized. Understanding the extent of Hg contamination and recovery following decreases in atmospheric Hg deposition will require further study, underscoring the need to continue existing monitoring efforts.

Mercury exposure in songbird communities along an elevational gradient on Whiteface Mountain, Adirondack Park (New York, USA) - Amy K. Sauer; Charles T. Driscoll; David C. Evers; Evan M. Adams; Yang Yang (Ecotoxicology, 2020)

Mercury exposure in songbird communities within Sphagnum bog and upland forest ecosystems in the Adirondack Park (New York, USA) - Amy K. Sauer; Charles T. Driscoll; David C. Evers; Evan M. Adams; Yang Yang (Ecotoxicology, 2020)

The effects of climate, habitat, and trophic position on methylmercury bioavailability for breeding New York songbirds - Evan M. Adams; Amy K. Sauer; Oksana Lane; Kevin Regan; David C. Evers (Ecotoxicology, 2020)

Forget the audience: tadpoles release similar disturbance cues regardless of kinship or familiarity - Kevin R. Bairos-Novak; Adam L. Crane; Gabrielle H. Achtymichuk; Jonathan Hsin; Ita A. E. Rivera-Hernández; Olena M. Simko; Theresa E. Wrynn; Douglas P. Chivers; Maud C. O. Ferrari (Behavioral Ecology and Sociobiology, 2020)

Ameliorative effect of extract of Tecoma stans (L.) Juss. ex kunth leaves against CCl4 - and acetaminophen—induced liver damage in rats - C. Larbie; B. O. Emikpe; S. A. Akpor; E. Adams; C. O. Adjei; A. A. Oyagbemi; T. A. Jarikre (Advances in Traditional Medicine, 2020)

Systematic map of conservation psychology - Kenneth E. Wallen; Adam C. Landon (Conservation Biology, 2020)
Conservation science and practice commonly draw on the theories and methods of social psychology to explain human cognition, emotion, and behavior germane to biodiversity conservation. We created a systematic map of the cross‐disciplinary conservation science literature, which draws on social psychology concepts and methods in their application broadly described as conservation psychology. Established protocols were used to systematically collect and collate peer‐reviewed research published in an explicit selection of multidisciplinary conservation journals. We sought to catalog the literature, elucidate trends and gaps, and critically reflect on the state of conservation psychology and its research practices that aim to influence conservation outcomes. The volume of publications per year and per decade increased from 1974 to 2016. Although a diversity of research designs and methods was applied, studies disproportionately focused on specific concepts (attitudes and beliefs), locations (North America and Europe), and contexts (terrestrial, rural). Studies also tended to be descriptive, quantitative, and atheoretical in nature. Our findings demonstrate that although conservation psychology has generally become more visible and prominent, it has done so within a limited space and suggest that disciplinary research principles and reporting standards must be more universally adopted by traditional and multidisciplinary conservation journals to raise the floor of empirical research.

Hurricane Sandy Effects on Coastal Marsh Elevation Change - Alice G. Yeates; James B. Grace; Jennifer H. Olker; Glenn R. Guntenspergen; Donald R. Cahoon; Susan Adamowicz; Shimon C. Anisfeld; Nels Barrett; Alice Benzecry; Linda Blum; Robert R. Christian; Joseph Grzyb; et al. (Estuaries and Coasts, 2020)

Ultraviolet-assisted oiling assessment improves detection of oiled birds experiencing clinical signs of hemolytic anemia after exposure to the Deepwater Horizon oil spill - Jesse A. Fallon; Eric P. Smith; Nina Schoch; James D. Paruk; Evan M. Adams; David C. Evers; Patrick G. R. Jodice; Marie Perkins; Dustin E. Meattey; William A. Hopkins (Ecotoxicology, 2020)

High Energy Side and Rear American Football Head Impacts Cause Obvious Performance Decrement on Video - Adam J. Bartsch; Daniel Hedin; Jay Alberts; Edward C. Benzel; Jason Cruickshank; Robert S. Gray; Kenneth Cameron; Megan N. Houston; Tyler Rooks; Gerald McGinty; Erick Kozlowski; Steven Rowson; et al. (Annals of Biomedical Engineering, 2020)

Clear as mud: the ecology and conservation of a secretive wetland fish (Neochanna cleaveri:Galaxiidae) in a heavily altered landscape - Nick S. Whiterod; Michael P. Hammer; Thomas C. Barnes; Mel Tucker; Mark Adams; Tarmo A. Raadik (Wetlands Ecology and Management, 2020)

Acoustic Speech Analytics Are Predictive of Cerebellar Dysfunction in Multiple Sclerosis - Gustavo Noffs; Frederique M. C. Boonstra; Thushara Perera; Scott C. Kolbe; Jim Stankovich; Helmut Butzkueven; Andrew Evans; Adam P. Vogel; Anneke van der Walt (The Cerebellum, 2020)

Predicting site-specific storm wave run-up - Julia W. Fiedler; Adam P. Young; Bonnie C. Ludka; William C. O’Reilly; Cassandra Henderson; Mark A. Merrifield; R. T. Guza (Natural Hazards, 2020)
Storm wave run-up causes beach erosion, wave overtopping, and street flooding. Extreme runup estimates may be improved, relative to predictions from general empirical formulae with default parameter values, by using historical storm waves and eroded profiles in numerical runup simulations. A climatology of storm wave run-up at Imperial Beach, California is developed using the numerical model SWASH, and over a decade of hindcast spectral waves and observed depth profiles. For use in a local flood warning system, the relationship between incident wave energy spectra E ( f ) and SWASH-modeled shoreline water levels is approximated with the numerically simple integrated power law approximation (IPA). Broad and multi-peaked E ( f ) are accommodated by characterizing wave forcing with frequency-weighted integrals of E ( f ). This integral approach improves runup estimates compared to the more commonly used bulk parameterization using deep water wave height $$H_0$$ H 0 and deep water wavelength $$L_0$$ L 0 Hunt (Trans Am Soc Civ Eng 126(4):542–570, 1961) and Stockdon et al. (Coast Eng 53(7):573–588, 2006. 10.1016/j.coastaleng.2005.12.005 ). Scaling of energy and frequency contributions in IPA, determined by searching parameter space for the best fit to SWASH, show an $$H_0L_0$$ H 0 L 0 scaling is near optimal. IPA performance is tested with LiDAR observations of storm run-up, which reached 2.5 m above the offshore water level, overtopped backshore riprap, and eroded the foreshore beach slope. Driven with estimates from a regional wave model and observed $$β _f$$ β f , the IPA reproduced observed run-up with $$<30%$$ < 30 % error. However, errors in model physics, depth profile, and incoming wave predictions partially cancelled. IPA (or alternative empirical forms) can be calibrated (using SWASH or similar) for sites where historical waves and eroded bathymetry are available.

Temporally Altered miRNA Expression in a Piglet Model of Hypoxic Ischemic Brain Injury - Sophie Casey; Kate Goasdoue; Stephanie M. Miller; Gary P. Brennan; Gary Cowin; Adam G. O’Mahony; Christopher Burke; Boubou Hallberg; Geraldine B. Boylan; Aideen M. Sullivan; David C. Henshall; Gerard W. O’Keeffe; et al. (Molecular Neurobiology, 2020)

Formation of the Mantoverde iron oxide-copper-gold (IOCG) deposit, Chile: insights from Fe and O stable isotopes and comparisons with iron oxide-apatite (IOA) deposits - Tristan M. Childress; Adam C. Simon; Martin Reich; Fernando Barra; Mauricio Arce; Craig C. Lundstrom; Ilya N. Bindeman (Mineralium Deposita, 2020)

Divergent trends of large carnivore populations within the Bénoué Complex, North Cameroon, shown by long-term fine-scale monitoring - C. E. Bakker; I. E. Kirsten; H. Bauer; B. M. Croes; W. L. M. Tamis; P. N. Tumenta; S. Adam; A. S. Kamgang; H. H. de Iongh (European Journal of Wildlife Research, 2020)

Mercury exposure in migrating songbirds: correlations with physical condition - Evan M. Adams; Kathryn A. Williams; Brian J. Olsen; David C. Evers (Ecotoxicology, 2020)

A preliminary assessment of mercury in the feathers of migratory songbirds breeding in the North American subarctic - I. J. Stenhouse; E. M. Adams; L. M. Phillips; S. Weidensaul; C. L. McIntyre (Ecotoxicology, 2020)

Historical patterns in mercury exposure for North American songbirds - Marie Perkins; Oksana P. Lane; David C. Evers; Amy Sauer; Evan M. Adams; Nelson J. O’Driscoll; Samuel T. Edmunds; Allyson K. Jackson; Julie C. Hagelin; Jeremiah Trimble; Elsie M. Sunderland (Ecotoxicology, 2020)

Geographic hierarchical population genetic structuring in British European whitefish (Coregonus lavaretus) and its implications for conservation - M. Crotti; C. E. Adams; E. C. Etheridge; C. W. Bean; A. R. D. Gowans; R. Knudsen; A. A. Lyle; P. S. Maitland; I. J. Winfield; K. R. Elmer; K. Præbel (Conservation Genetics, 2020)
The European whitefish Coregonus lavaretus complex represents one of the most diverse radiations within salmonids, with extreme morphological and genetic differentiation across its range. Such variation has led to the assignment of many populations to separate species. In Great Britain, the seven native populations of C. lavaretus (two in Scotland, four in England, one in Wales) were previously classified into three species, and recent taxonomic revision resurrected the previous nomenclature. Here we used a dataset of 15 microsatellites to: (1) investigate the genetic diversity of British populations, (2) assess the level of population structure and the relationships between British populations. Genetic diversity was highest in Welsh (H O = 0.50, A R = 5.29), intermediate in English (H O = 0.41–0.50, A R = 2.83–3.88), and lowest in Scottish populations (H O = 0.28–0.35, A R = 2.56–3.04). Population structure analyses indicated high genetic differentiation (global F ST = 0.388) between all populations but for the two Scottish populations (F ST = 0.063) and two English populations (F ST = 0.038). Principal component analysis and molecular ANOVA revealed separation between Scottish, English, and Welsh populations, with the Scottish populations being the most diverged. We argue that the data presented here are not sufficient to support a separation of the British European whitefish populations into three separate species, but support the delineation of different ESUs for these populations.

An Overview of Drug Substance Manufacturing Processes - Noah J. Wichrowski; Adam C. Fisher; Nilou S. Arden; Xiaochuan Yang (AAPS PharmSciTech, 2020)

Spatial, climate and ploidy factors drive genomic diversity and resilience in the widespread grass Themeda triandra - Collin W. Ahrens; Elizabeth A. James; Adam D. Miller; Ferguson Scott; Nicola C. Aitken; Ashley W. Jones; Patricia Lu‐Irving; Justin O. Borevitz; David J. Cantrill; Paul D. Rymer (Molecular Ecology, 2020)
Global climate change poses a significant threat to natural communities around the world, with many plant species showing signs of climate stress. Grassland ecosystems are not an exception, with climate change compounding contemporary pressures such as habitat loss and fragmentation. In this study, we assess the climate resilience of Themeda triandra , a foundational species and the most widespread plant in Australia, by assessing the relative contributions of spatial, environmental and ploidy factors to contemporary genomic variation. Reduced‐representation genome sequencing on 472 samples from 52 locations was used to test how the distribution of genomic variation, including ploidy polymorphism, supports adaptation to hotter and drier climates. We explicitly quantified isolation by distance (IBD) and isolation by environment (IBE) and predicted genomic vulnerability of populations to future climates based on expected deviation from current genomic composition. We found that a majority (54%) of genomic variation could be attributed to IBD, while an additional 22% (27% when including ploidy information) could be explained by two temperature and two precipitation climate variables demonstrating IBE. Ploidy polymorphisms were common within populations (31/52 populations), indicating that ploidy mixing is characteristic of T. triandra populations. Genomic vulnerabilities were found to be heterogeneously distributed throughout the landscape, and our analysis suggested that ploidy polymorphism, along with other factors linked to polyploidy, reduced vulnerability to future climates by 60% (0.25–0.10). Our data suggests that polyploidy may facilitate adaptation to hotter climates and highlight the importance of incorporating ploidy in adaptive management strategies to promote the resilience of this and other foundation species.

Modelling climate change impacts on maize yields under low nitrogen input conditions in sub‐Saharan Africa - Gatien N. Falconnier; Marc Corbeels; Kenneth J. Boote; François Affholder; Myriam Adam; Dilys S. MacCarthy; Alex C. Ruane; Claas Nendel; Anthony M. Whitbread; Éric Justes; Lajpat R. Ahuja; Folorunso M. Akinseye; et al. (Global Change Biology, 2020)
Smallholder farmers in sub‐Saharan Africa (SSA) currently grow rainfed maize with limited inputs including fertilizer. Climate change may exacerbate current production constraints. Crop models can help quantify the potential impact of climate change on maize yields, but a comprehensive multimodel assessment of simulation accuracy and uncertainty in these low‐input systems is currently lacking. We evaluated the impact of varying [CO 2 ], temperature and rainfall conditions on maize yield, for different nitrogen (N) inputs (0, 80, 160 kg N/ha) for five environments in SSA, including cool subhumid Ethiopia, cool semi‐arid Rwanda, hot subhumid Ghana and hot semi‐arid Mali and Benin using an ensemble of 25 maize models. Models were calibrated with measured grain yield, plant biomass, plant N, leaf area index, harvest index and in‐season soil water content from 2‐year experiments in each country to assess their ability to simulate observed yield. Simulated responses to climate change factors were explored and compared between models. Calibrated models reproduced measured grain yield variations well with average relative root mean square error of 26%, although uncertainty in model prediction was substantial (CV = 28%). Model ensembles gave greater accuracy than any model taken at random. Nitrogen fertilization controlled the response to variations in [CO 2 ], temperature and rainfall. Without N fertilizer input, maize (a) benefited less from an increase in atmospheric [CO 2 ]; (b) was less affected by higher temperature or decreasing rainfall; and (c) was more affected by increased rainfall because N leaching was more critical. The model intercomparison revealed that simulation of daily soil N supply and N leaching plays a crucial role in simulating climate change impacts for low‐input systems. Climate change and N input interactions have strong implications for the design of robust adaptation approaches across SSA, because the impact of climate change in low input systems will be modified if farmers intensify maize production with balanced nutrient management.

A Morphological Method to Approximate Jumping Performance in Anurans for Macroevolutionary Studies - Bryan H. Juarez; Daniel S. Moen; Dean C. Adams (Evolutionary Biology, 2020)

Making Heads or Tails of Combined Landmark Configurations in Geometric Morphometric Data - Michael L. Collyer; Mark A. Davis; Dean C. Adams (Evolutionary Biology, 2020)

Younger Dryas and early Holocene subsistence in the northern Great Basin: multiproxy analysis of coprolites from the Paisley Caves, Oregon, USA - John C. Blong; Martin E. Adams; Gabriel Sanchez; Dennis L. Jenkins; Ian D. Bull; Lisa-Marie Shillito (Archaeological and Anthropological Sciences, 2020)
Younger Dryas and early Holocene Western Stemmed Tradition occupants of the northern Great Basin appear to have practiced a broad-based subsistence strategy including the consumption of a wide variety of small animal and plant resources. However, much of our evidence for human diet and land use during this period comes from dry cave and rockshelter sites where it can be challenging to distinguish plant and small animal remains deposited as a result of human versus nonhuman activity. This study presents new direct evidence for Younger Dryas and early Holocene human diet in the northern Great Basin through multiproxy analysis of nine human coprolites from the Paisley Caves, Oregon, USA. The evidence indicates that Western Stemmed Tradition occupants consumed plants, small mammals, fish, and insects, including direct evidence for consumption of whole rodents and several types of beetle. Occupation of the caves occurred during the summer and fall by individuals foraging on wetland, sagebrush grassland, and riparian ecological landscapes suggesting geographical and seasonal variability in land-use patterns during the Younger Dryas and early Holocene periods. This research suggests that Western Stemmed Tradition settlement patterns were seasonally centered on productive valley bottom lakes and wetlands but also included forays to a variety of ecological landscapes. The results highlight the importance of plant and small animal resources in the human diet during the terminal Pleistocene settlement of North America and contribute to debates about the process of the peopling of the Americas.

Additive effect of erythropoietin use on exercise-induced endothelial activation and hypercoagulability in athletes - Jules A. A. C. Heuberger; Jelle J. Posthuma; Dimitrios Ziagkos; Joris I. Rotmans; Johannes M. A. Daniels; Pim Gal; Frederik E. Stuurman; Henri M. H. Spronk; Hugo Ten Cate; Jacobus Burggraaf; Matthijs Moerland; Adam F. Cohen (European Journal of Applied Physiology, 2020)
Purpose Recombinant human erythropoietin (rHuEPO) is known to increase thrombotic risk in patients and might have similar effects in athletes abusing the drug. rHuEPO is prohibited by anti-doping legislation, but this risk has not been investigated thoroughly. This analysis was designed to evaluate whether rHuEPO impacts hemostatic profile and endothelial and platelet activation markers in trained subjects, and whether the combination with exercise affects exercise induced alterations. Methods This double-blind, randomized, placebo-controlled trial enrolled healthy, trained male cyclists aged 18–50 years. Participants were randomly allocated (1:1) to receive subcutaneous injections of rHuEPO (epoetin-β; mean dose 6000 IU per week) or placebo (0.9% NaCl) for 8 weeks. Subjects performed five maximal exercise tests and a road race, coagulation and endothelial/platelet markers were measured at rest and directly after each exercise effort. Results rHuEPO increased P-selectin (+ 7.8% (1.5–14.5), p = 0.02) and E-selectin (+ 8.6% (2.0–15.7), p = 0.01) levels at rest. Maximal exercise tests significantly influenced all measured coagulation and endothelial/platelet markers, and in the rHuEPO group maximal exercise tests led to 15.3% ((7.0–24.3%), p = 0.0004) higher E-selectin and 32.1% ((4.6–66.8%), p = 0.0207) higher Platelet factor 4 (PF4) levels compared to the placebo group. Conclusion In conclusion, rHuEPO treatment resulted in elevated E- and P-selectin levels in trained cyclists, indicating enhanced endothelial activation and/or platelet reactivity. Exercise itself induces hypercoagulability, and the combination of rHuEPO and exercise increased E-selectin and PF4 levels more than either intervention alone. Based on this, exercise potentially increases thrombotic risk, a risk that might be enhanced in combination with rHuEPO use.

Normative Influences on Farmers’ Intentions to Practice Conservation Without Compensation - Jerry J. Vaske; Adam C. Landon; Craig A. Miller (Environmental Management, 2020)

Experimental investigation of phase relations in the system NaAlSi3O8–H2O at high temperatures and pressures: liquidus relations, liquid–vapor mixing, and critical phenomena at deep crust–upper mantle conditions - Adam R. Makhluf; R. C. Newton; C. E. Manning (Contributions to Mineralogy and Petrology, 2020)

The value of EDTA treatment of hatchery water to rear Greenshell™ mussel (Perna canaliculus) larvae - Daniel R. McDougall; Julien Vignier; Norman L. C. Ragg; Bridget Finnie; Andrew Jeffs; Serean Adams (Aquaculture International, 2020)

Plio‐Pleistocene sea‐level changes drive speciation of freshwater fishes in north‐western Australia - James J. Shelley; Stephen E. Swearer; Tim Dempster; Mark Adams; Matthew C. Le Feuvre; Michael P. Hammer; Peter J. Unmack (Journal of Biogeography, 2020)
Aim Despite the influence of sea‐level changes on biogeographic/phylogeographic patterns in freshwater ecosystems being well documented, studies that explicitly link the influence of sea‐level change with speciation are rare. We aim to test the hypothesis that sea‐level changes during the Pliocene and Pleistocene have driven speciation in north‐western Australia's (NWA’s) largest freshwater fish family, Terapontidae, building upon a body of evolutionary literature focussed on the family. Location North‐western Australian rivers including those draining the Kimberley Plateau. Taxon Grunters (Family: Terapontidae, Genera: Hannia , Hephaestus , Leiopotherapon , Syncomistes ). Methods A GIS was used to reconstruct palaeodrainages during lowered sea levels and to delineate regions of high connectivity during low and high (current) sea‐level conditions. For seven species, the degree of phylogenetic divergence among river basins in different regions was evaluated using a maximum likelihood phylogeny and analyses of the proportion of genetic divergence expressed with 601 base pairs of the mtDNA cytochrome b (cytb) gene. Results A low proportion of cytb haplotypes were shared among catchments not connected by the same receiving waters (e.g. estuaries) under current (high) sea levels, indicating that contemporary dispersal is limited over fine spatial scales. Deeper phylogeographic patterns were largely congruent with reconstructed low sea‐level (LSL) drainage arrangements indicating that historic among‐catchment connectivity was far more widespread under LSL conditions. Main Conclusions The NWA landscape represents a geographic template that has shaped patterns of broad dispersal under low sea levels, and fine‐scale isolation under high sea levels. The weight of evidence from recent literature on species boundaries and evolutionary patterns within the terapontids suggests that most NWA species were derived rapidly and recently from a series of spatio‐temporal vicariant events caused by such sea‐level fluctuations during the late Pliocene and Pleistocene. Together, the findings provide a rare, comprehensively tested example of sea‐level change driving speciation in the tropics.

Effects of body size on estimation of mammalian area requirements - Michael J. Noonan; Christen H. Fleming; Marlee A. Tucker; Roland Kays; Autumn‐Lynn Harrison; Margaret C. Crofoot; Briana Abrahms; Susan C. Alberts; Abdullahi H. Ali; Jeanne Altmann; Pamela Castro Antunes; Nina Attias; et al. (Conservation Biology, 2020)
Accurately quantifying species’ area requirements is a prerequisite for effective area‐based conservation. This typically involves collecting tracking data on species of interest and then conducting home‐range analyses. Problematically, autocorrelation in tracking data can result in space needs being severely underestimated. Based on the previous work, we hypothesized the magnitude of underestimation varies with body mass, a relationship that could have serious conservation implications. To evaluate this hypothesis for terrestrial mammals, we estimated home‐range areas with global positioning system (GPS) locations from 757 individuals across 61 globally distributed mammalian species with body masses ranging from 0.4 to 4000 kg. We then applied block cross‐validation to quantify bias in empirical home‐range estimates. Area requirements of mammals 1, meaning the scaling of the relationship changed substantially at the upper end of the mass spectrum.