New nighttime light landscape metrics for analyzing urban-rural differentiation in economic development at township: A case study of Fujian province, China - Zuoqi Chen; Siyi Yu; Xiaojun You; Chengshu Yang; Congxiao Wang; Juan Lin; Wenting Wu; Bailang Yu (Applied Geography, 2023)

Characterising the influence of acid rain on the growth and physiological characteristics of Mirabilis jalapa Linn. in southern China - Lan-ying Chen; Lin Wang; Hao-yu Wang; Xun Zhu (Acta Physiologiae Plantarum, 2023)

Physiological and transcriptome analysis of Mytilus coruscus in response to Prorocentrum lima and microplastics - Xiao-wen Tang; Rong Yu; Mei-hua Fan; Zhi Yang; Zhi Liao; Zong-xin Yang; Chen-ying Xie; Yu-kun Xuan; Jian-xin Wang; Xiao-lin Zhang; Xiao-jun Yan (Frontiers in Marine Science, 2022)
Nowadays, diarrheic shellfish toxicity (DSP) toxin and microplastics (MPs) are commonly found in coastal waters worldwide. Due to their widespread use, their persistence and toxicity, they may induce adverse effects on Mytilus coruscus . However, the underlying toxic mechanisms of DSP and MPs on M. coruscus remain unclear. This study explored the physiological index and transcriptome change of the digestive gland of adult M. coruscus exposed for 3 days to polystyrene (PS) MPs (0.2 mg/L, 90-100 μm) and Prorocentrum lima alone or in combination. The results showed that the CAT activity and MDA content significantly increased, respiration rate and feeding rate significantly decreased. The combination of MPs and P. lima caused more structural damage to the rough surface endoplasmic reticulum and mitochondria in the digestive glands of M. coruscus . The transcriptome analysis showed that 485 and 220 genes were up- and down-regulated, respectively, after exposure to P. lima ; 1,989 up-regulated DEGs and 1,098 down-regulated DEGs were identified after exposure to MP treatment, and 1,004 up-regulated DEGs and 664 down-regulated DEGs were identified after exposure to the combination of P. lima and MPs. The DEGs were mainly enriched in the lysosome, mRNA surveillance pathway, carbon metabolism, the mTOR signaling pathway, the complement and coagulation cascades, and the TNF signaling pathway. The MP, P. lima exposure mainly induced the expression of RNA-binding protein musashi, serine/arginine repetitive matrix protein 1, low affinity immunoglobulin epsilon Fc receptor, toll-like receptor 2, caspase 7, calmodulin, E3 ubiquitin-protein ligase, serine/threonine-protein kinase PRP4, glutathione S-transferase, and heat shock 70 kDa protein. MPs and P. lima poison mainly influence the expression of RNA transport, immune related gene, apoptosis, signal related gene, and antioxidant gene change. The combination of MPs and P. lima has a synergistic toxic effect. This study provides a new insights into its physiological and molecular responses of M. coruscus to MPs and P. lima toxic exposure.

Rapid quantitative study of check dam breach floods under extreme rainstorm - Qiang Zhang; Yanlong Li; Shu Yu; Lin Wang; Zuyu Chen; Jiawei Zhou (Natural Hazards, 2022)

Phycospheric bacterial community structure and function succession during the typical harmful macroalgal blooms - Tongfei Qu; Chengzong Hou; Chen Guan; Xinyu Zhao; Jun Chen; Yi Zhong; Jinhui Xu; Zhihao Lin; Yu Xu; Xuexi Tang; Ying Wang (Frontiers in Marine Science, 2022)
Based on the diversity and importance of phycospheric microorganisms as well as their complex interaction with algae, their ecological correlation with algal bloom events has become a research topic of great interest that remains unclear in the natural dynamic process of harmful macroalgal blooms (HMBs). The world’s largest green tides caused by macroalgae Ulva prolifera have occurred in the Yellow Sea for 16 consecutive years, and seriously affected the coastal ecosystem. Here, we monitored the spatiotemporal dynamics of the phycospheric bacterial community during the U . prolifera green tide bloom. The 73-day continuous field survey covered the whole process of initial invasion until the extinction of the green tide. The phycospheric bacterial community has a higher richness than the control seawater bacteria; in addition, it has more enriched taxa with organic nutrition preference and environmental pressure adaption, such as Bacteroidetes, Firmicutes, and Desulfobacterota. The same differences in populations and functional profiles were observed among the epiphytic bacterial and phycospheric seawater bacterial communities, which may be driven by the spatial heterogeneity of biogenic elements (e.g., organic nutrients and oxygen) among the habitats. Significant succession occurred in both the epiphytic bacterial and phycospheric seawater bacterial communities; their diversity and richness exhibited significant heterogeneity variation patterns, and their community structure became more similar in late phases. Our study revealed that macroalgal phycospheric microbial communities have a highly complex and dynamic composition and variable ecological functions, which indicate that phycospheric microorganisms are closely related to the fate of HMBs and may have the profound effect coastal biogeochemical cycles.

Associations between residential proximity to agricultural land use as pesticides exposure and birth outcomes - Kuna-Chun Chen; Shuo-Fang Lee; Shyang-Woei Lin; Jia-Shan Xie; Yu‐Hsun Chang; Lih-Ming Yiin; Chia-Jung Hsieh (Environmental Science and Pollution Research, 2022)

Mesenchymal Stem Cell Secreted-Extracellular Vesicles are Involved in Chondrocyte Production and Reduce Adipogenesis during Stem Cell Differentiation - Yu-Chen Tsai; Tai-Shan Cheng; Hsiu-Jung Liao; Ming-Hsi Chuang; Hui-Ting Chen; Chun-Hung Chen; Kai-Ling Zhang; Chih-Hung Chang; Po-Cheng Lin; Chi-Ying F. Huang (Tissue Engineering and Regenerative Medicine, 2022)
Background: Extracellular vesicles (EVs) are derived from internal cellular compartments, and have potential as a diagnostic and therapeutic tool in degenerative disease associated with aging. Mesenchymal stem cells (MSCs) have become a promising tool for functional EVs production. This study investigated the efficacy of EVs and its effect on differentiation capacity. Methods: The characteristics of MSCs were evaluated by flow cytometry and stem cell differentiation analysis, and a production mode of functional EVs was scaled from MSCs. The concentration and size of EVs were quantitated by Nanoparticle Tracking Analysis (NTA). Western blot analysis was used to assess the protein expression of exosome-specific markers. The effects of MSC-derived EVs were assessed by chondrogenic and adipogenic differentiation analyses and histological observation. Results: The range of the particle size of adipose-derived stem cells (ADSCs)- and Wharton’s jelly -MSCs-derived EVs were from 130 to 150 nm as measured by NTA, which showed positive expression of exosomal markers. The chondrogenic induction ability was weakened in the absence of EVs in vitro . Interestingly, after EV administration, type II collagen, a major component in the cartilage extracellular matrix, was upregulated compared to the EV-free condition. Moreover, EVs decreased the lipid accumulation rate during adipogenic induction. Conclusion: The results indicated that the production model could facilitate production of effective EVs and further demonstrated the role of MSC-derived EVs in cell differentiation. MSC-derived EVs could be successfully used in cell-free therapy to guide chondrogenic differentiation of ADSC for future clinical applications in cartilage regeneration.

Combined analysis of mRNA and miRNA reveals the banana potassium absorption regulatory network and validation of miRNA160a - Wenliang Chen; Tao Dong; Yinglong Chen; Ping Lin; Chuqiao Wang; Kelin Chen; Yi Tang; Mingyuan Wang; Jianfu Liu; Hailing Yu (Plant Molecular Biology, 2022)

Shanghai Autism Early Development: An Integrative Chinese ASD Cohort - Yuan Dai; Yuqi Liu; Lingli Zhang; Tai Ren; Hui Wang; Juehua Yu; Xin Liu; Zilin Chen; Lin Deng; Minyi Tao; Hangyu Tan; Chu-Chung Huang; et al. (Neuroscience Bulletin, 2022)

Copper Toxicity Differentially Regulates the Seedling Growth, Copper Distribution, and Photosynthetic Performance of Citrus sinensis and Citrus grandis - Xin-yu Li; Mei-lan Lin; Ping-ping Hu; Ning-wei Lai; Zeng-rong Huang; Li-song Chen (Journal of Plant Growth Regulation, 2022)

AIoT-Based Ergometer for Physical Training in Frail Elderly with Cognitive Decline: A Pilot Randomized Control Trial - Chih-Chun Lin; Li-Chieh Kuo; Yu-Sheng Lin; Chia-Ming Chang; Fang Wen Hu; Yi-Jing Chen; Chun-Tse Lin; Fong-Chin Su (Journal of Medical and Biological Engineering, 2022)
Purpose Reduced physical activity is reported in the elderly, especially in institutional residents. Institutionalized older adults exhibit a high prevalence of frailty. In this work, we developed an artificial intelligence of things (AIoT)-based feedback assistive strengthening ergometer (AIFASE), for the physical strengthening of the elderly with intelligent assistance. Methods We conducted a 12-week intervention in a long-term care facility. In total, 16 participants (84.38 ± 6.0 years; 4 males and 12 females) were recruited with 1:1 randomization of exercise to control groups. The muscle strength of the lower extremities, timed up and go test (TUG), and Short-form Physical Performance Battery (SPPB) of the participants were measured. The AIFASE system allows the clinical staff to record the personal physical performance of the elderly and generates personalized exercise prescriptions accordingly. AIFASE also displays the current usage status of all ergometers and the users’ physiological conditions. The algorithms were developed to generate warning alerts when the training workload was too large by personal physiological detection. AIFASE automatically customized the exercise prescription according to the user’s exercise performance. Results After a 12-week AIFASE intervention, the intervention group exhibited significant improvements in the strength of the hip flexor, Semi-Tandem Stand, and Tandem Stand. Conclusion In this study, we developed an AIoT ergometer that delivered customized physical training prescriptions to improve the physical performance of long-term care facility residents. We believe that the application of AIFASE will help improve the quality of institutional care.

Locus-level antagonistic selection shaped the polygenic architecture of human complex diseases - Weichen Song; Kai Yuan; Zhe Liu; Wenxiang Cai; Jue Chen; Shunying Yu; Min Zhao; Guan Ning Lin (Human Genetics, 2022)

An association study on the risk, glucocorticoids effectiveness, and prognosis of systemic lupus erythematosus: insight from mitochondrial DNA copy number - Zhen Li; Qi-Qun Zong; Chun-Xia Zhai; Guang-Hui Yu; Wan-Qin Hu; Yu-Hua Wang; Lin-Lin Wang; Zi-Ye Yan; Ting-Yu Zhang; Ying Teng; Shuang Liu; Jing Cai; et al. (Immunologic Research, 2022)

Orientin downregulating oxidative stress-mediated endoplasmic reticulum stress and mitochondrial dysfunction through AMPK/SIRT1 pathway in rat nucleus pulposus cells in vitro and attenuated intervertebral disc degeneration in vivo - Zhao Zhang; Jingtao Wu; Cheng Teng; Jinquan Wang; Jiapei Yu; Chen Jin; Libo Wang; Long Wu; Zhen Lin; Ziping Yu; Zhongke Lin (Apoptosis, 2022)

Potential risk from and prevention of phytoplankton outbreaks in blocking the cooling water system in a nuclear power plant on the Southeast China coast - Yu Wang; Xiaoyin Chen; Yingyue Lin; Shuyi Zhang; Lin Chang; Xiaoming Tang; Peng Xiang; Heshan Lin (Frontiers in Marine Science, 2022)
The invasion or aggregation of marine biota in coastal waters has become a common problem, threatening the safety of cooling water systems in the context of environmental and climate changes. However, studies on issues of coastal nuclear power plants from the view of marine ecology have mostly focused on the thermal effluent, entrainment effect on organisms, residual chlorine, radioactivity, etc. There is a lack of information on the safety of cooling water systems in nuclear power plants blocked by marine biota. Based on field and historical datasets, this study screened phytoplankton species that may potentially block the cooling water system of the Fuqing nuclear power plant on the Southeast China coast, identified the phytoplankton risk and a risk calendar, and established a catalog of risk phytoplankton species for the cooling water system. The screening criteria were simultaneously required as follows: (1) particle size of cells or group >2000 μm; (2) phytoplankton bloom or aggregation events occurred in surrounding areas; (3) and species abundance maintained at >0.5×10 5 cells/L in the surrounding area. Nine high-risk species were screened out that can easily form colonies, clusters, and groups, including Thalassiosira diporocyclus , Thalassiosira subtilis , Noctiluca scintillans , Phaeocystis globosa , Pseudo-nitzschia pungens , Pseudo-nitzschia delicatissima , Skeletonema costatum , Skeletonema dohrnii , Paralia sulcata . Due to small cells or the formation of short chains, some dominant phytoplankton species, even those with advantages in abundance and occurrence, had difficulty threatening the reliability of cooling water systems in nuclear power plants. The outbreak of different phytoplankton species varied daily, and the risk of blockage was highest from April to July. The biological characteristics, ecological mechanisms underlying outbreaks of high-risk phytoplankton species are reviewed and discussed, suggesting a high degree of temporal and spatial variability of blockage risk. Finally, main prevention and control measures used against high-risk phytoplankton species are provided to imply new ideas and methods for nuclear power plants. These strategies have very important practical and scientific significance.

Age, Growth and Population Structure Analyses of the Purpleback Flying Squid Sthenoteuthis oualaniensis in the Northwest Indian Ocean by the Statolith Microstructure - Yu-Zhe Ou; Hua-Jie Lu; Hong-Hao Wang; Zi-Yue Chen; Mao-Lin Zhao (Fishes, 2022)
A total of 1177 Sthenoteuthis oualaniensis were randomly collected from the northwest Indian Ocean from February between May 2019 and 2020 by lighting falling-net vessels. The age, growth, and population structure of S. oualaniensis were studied based on the statolith microstructure. The results showed that the range of mantle length (ML) was 123–562 mm for females and 88–273 mm for males, and the range of body weight (BW) was 78–6268 g for females and 82–518 g for males in 2019 and 2020, respectively. The hatching date extended from May to December, with the 2019 samples mainly composed of the autumn population, while the 2020 samples were mostly composed of the summer population. The analysis of covariance (ANCOVA) showed that there were significant differences in the growth of ML–age and BW–age between sexes. In the growth model of the ML–age relationship, both females and males in 2019 were best described by s linear model, and 2020 was best described by a logarithmic model. The growth model of the BW–age relationship of females and males in 2019 was best described as linear and the growth model of the BW–age relationship of females and males in 2020 was described logarithmically and exponentially, respectively. The average absolute daily growth rate (AGR) and instantaneous growth rate (IGR) for ML of the 2019 samples were 0.85 mm/d and 0.40%/d, and the average AGR and IGR for ML of the 2020 samples were 0.65 mm/d and 0.18%, respectively. The growth of S. oualaniensis samples in 2019 was faster than that in 2020. This study provided basic information on the age, growth, and population of S. oualaniensis, which will supply a scientific basis for stock assessment and sustainable development.

Protective effect of ginsenoside Rd on military aviation noise-induced cochlear hair cell damage in guinea pigs - Xue-min Chen; Yu-hui Liu; Shuai-fei Ji; Xin-miao Xue; Lin-lin Wang; Min Zhang; Yao-ming Chang; Xiao-cheng Wang (Environmental Science and Pollution Research, 2022)

Design of deep-sea chemical data collector for the seafloor observatory network - Bin Lv; Jie Chen; Hai-lin Liu; Chao Liu; Zhao-wen Zhang; Xiao-nan Zhang; Hao Gao; Yu-long Cai (Marine Georesources & Geotechnology, 2022)

A study of peatland-derived dissolved organic matter from headstream to sea using multiple analytical tools - François L. L. Muller; Kuei-Chen Chang; Yu-Chieh Wang; Chon-Lin Lee (Environmental Science and Pollution Research, 2022)

7,8-Dihydroxyflavone attenuates the virulence of Staphylococcus aureus by inhibiting alpha-hemolysin - Nan Bian; Xiangqian Chen; Xinran Ren; Zishu Yu; Mengli Jin; Xiaoyu Chen; Chang Liu; Yanhe Luan; Lin Wei; Ying Chen; Wu Song; Yicheng Zhao; et al. (World Journal of Microbiology and Biotechnology, 2022)

Novel and sustainable carboxylation of terminal alkynes and CO2 to alkynyl carboxylic acids using triazolium ionic liquid-modified PMO-supported transition metal acetylacetonate as effective cooperative catalysts - Jing-Rui Li; Chen Chen; Xiao-Bing Liu; Yu-Lin Hu (Environmental Science and Pollution Research, 2022)

Activity concentrations and bioconcentration factors (BCFs) of natural radionuclides (40 K, 226Ra, and 232Th) from cultivated substrates to mushrooms - Wei-Hsiang Huang; Chih-Chung Lin; Yung-Yu Liu; Chao-Min Huang; Yi-Lung Yeh; Ting-Chien Chen (Environmental Science and Pollution Research, 2022)

Numerical analysis of pile—slope stability and the soil arching around two adjacent piles - Chien-Yuan Chen; Chun-Kai Chang; Yu-Shan Lin (Journal of Mountain Science, 2022)

A histological study of vascular wall resident stem cells in venous malformations - Hou-Fu Xia; Wen-Qiang Lai; Gao-Hong Chen; Ye Li; Qi-Hui Xie; Yu-Lin Jia; Gang Chen; Yi-Fang Zhao (Cell and Tissue Research, 2022)

Associations of metal profiles in blood with thyroiditis: a cross-sectional study - Yaosheng Luo; Huixian Zeng; Yanshi Ye; Genfeng Yu; Cheng Song; Siyang Liu; Xingying Chen; Yuqi Jiang; Hualin Duan; Yue Li; Shengqing He; Zhi Chen; et al. (Environmental Science and Pollution Research, 2022)
Autoimmune thyroiditis (AIT) is increasingly common, and serological markers include thyroid peroxidase antibody (TPOAb) and thyroglobulin antibody (TgAb). To determine if selected metals influence thyroiditis antibody positivity, this cross-sectional study investigated associations between metals and thyroiditis antibody status. Healthy individuals ( n = 1104) completed a questionnaire and underwent checkups of anthropometric parameters, thyroid function status, and levels of seven metals in blood (magnesium, iron, calcium, copper, zinc, manganese, and lead). Associated profiles of glyco- and lipid metabolism were also established. Logistic regression and restricted cubic spline (RCS) regression analysis were applied to adjudge associations between metals and TPOAb and TgAb status. It was found that, after adjusting for likely cofounding factors, participants with antibody positivity had significantly lower serum concentrations of magnesium and iron. When serum magnesium levels were analyzed in quartiles, the odds ratios of quartile 4 were 0.329–fold (95% confidence interval (CI): 0.167–0647) and 0.259-fold (95% CI 0.177–0.574) that of quartile 1 regarding TPOAb and TgAb positivity ( P = 0.004, 0.003). After adjustment, the RCS analysis detected nonlinear associations between iron and TPOAb and TgAb positivity ( P < 0.01, both). In stratified analyses, these associations regarding magnesium and iron remained for women of reproductive age, but not for postmenopausal women and men. We conclude that lower serum levels of magnesium and iron are associated with incremental positivity of thyroiditis antibodies and may be among the most important metals contributing to AIT in women of reproductive age.

Effects of tillage and cropping sequences on crop production and environmental benefits in the North China Plain - Wen-Xuan Liu; Wen-Sheng Liu; Mu-Yu Yang; Yu-Xin Wei; Zhe Chen; Ahmad Latif Virk; Rattan Lal; Xin Zhao; Hai-Lin Zhang (Environmental Science and Pollution Research, 2022)

NAD+-capped RNAs are widespread in rice (Oryza sativa) and spatiotemporally modulated during development - Haijiao Dong; Xufeng Wang; Cong Tan; Lei Gao; Jie Cui; Lin Liu; Beixin Mo; Yongzhong Xing; Yu Yu; Xuemei Chen (Science China Life Sciences, 2022)

Identifying Patients with Epilepsy Having Depression/Anxiety Disorder Using Common Spatial Patterns of Functional EEG Networks - Yuanyuan Zhang; Yuhang Lin; Zhichuang Qu; Zhi Zhang; Daqing Guo; Xin Chen; Haifen Shu; Sixun Yu (Journal of Medical and Biological Engineering, 2022)

Fracture Mechanical Properties of Three—Dimensional Irregular Sandstone Particles Based on Digital-Image - Kai-wen Shi; Yu-jun Zuo; Wen-ji-bin Sun; Hao Liu; Jian-yun Lin; Tao-tao Li; Bin Chen; Lu-lin Zheng (Geotechnical and Geological Engineering, 2022)

Clinical profiles and antimicrobial resistance patterns of invasive Salmonella infections in children in China - Wen Song; Qingwen Shan; Yue Qiu; Xianyao Lin; Chunhui Zhu; Zhiqiang Zhuo; Caihong Wang; Jianning Tong; Rui Li; Chaomin Wan; Yu Zhu; Minxia Chen; et al. (European Journal of Clinical Microbiology & Infectious Diseases, 2022)
Invasive Salmonella infections result in a significant burden of disease including morbidity, mortality, and financial cost in many countries. Besides typhoid fever, the clinical impact of non-typhoid Salmonella infections is increasingly recognized with the improvement of laboratory detection capacity and techniques. A retrospective multicenter study was conducted to analyze the clinical profiles and antimicrobial resistance patterns of invasive Salmonella infections in hospitalized children in China during 2016–2018. A total of 130 children with invasive Salmonella infections were included with the median age of 12 months (range: 1–144 months). Seventy-nine percent of cases occurred between May and October. Pneumonia was the most common comorbidity in 33 (25.4%) patients. Meningitis and septic arthritis caused by nontyphoidal Salmonella (NTS) infections occurred in 12 (9.2%) patients and 5 (3.8%) patients. Patients < 12 months (OR: 16.04) and with septic shock (OR: 23.4), vomit (OR: 13.33), convulsion (OR: 15.86), C-reactive protein (CRP) ≥ 40 g/L (OR: 5.56), and a higher level of procalcitonin (PCT) (OR: 1.05) on admission were statistically associated to an increased risk of developing meningitis. Compared to 114 patients with NTS infections , 16 patients with typhoid fever presented with higher levels of CRP and PCT ( P < 0.05). The rates of resistance to ampicillin, sulfamethoxazole/trimethoprim, ciprofloxacin, and ceftriaxone among Salmonella Typhi and NTS isolates were 50% vs 57.3%, 9.1% vs 24.8%, 0% vs 11.2%, and 0% vs 9.9%, respectively. NTS has been the major cause of invasive Salmonella infections in Chinese children and can result in severe diseases. Antimicrobial resistance among NTS was more common.

Aberrant brain connectivity is associated with childhood maltreatment in individuals with major depressive disorder - Qianyi Luo; Juran Chen; Yuhong Li; Zhiyao Wu; Xinyi Lin; Jiazheng Yao; Huiwen Yu; Huawang Wu; Hongjun Peng (Brain Imaging and Behavior, 2022)

Streptococcus humanilactis sp.nov., isolated from healthy nursing mother's breast milk - Xiaoqing Guo; Fei Han; Lin Cong; Yu Li; Yue Wu; Chen Liu; Jingfang Yang; Risu Na; Wenjun Liu (Archives of Microbiology, 2022)

The Super-large Ensemble Experiments of CAS FGOALS-g3 - Pengfei Lin; Bowen Zhao; Jilin Wei; Hailong Liu; Wenxia Zhang; Xiaolong Chen; Jie Jiang; Mengrong Ding; Wenmin Man; Jinrong Jiang; Xu Zhang; Yuewen Ding; et al. (Advances in Atmospheric Sciences, 2022)
A super-large ensemble simulation dataset with 110 members has been produced by the fully coupled model FGOALS-g3 developed by researchers at the Institute of Atmospheric Physics, Chinese Academy of Sciences. This is the first dataset of large ensemble simulations with a climate system model developed by a Chinese modeling center. The simulation has the largest realizations up to now worldwide in terms of single-model initial-condition large ensembles. Each member includes a historical experiment (1850–2014) and an experiment (2015–99) under the very high greenhouse gas emissions Shared Socioeconomic Pathway scenario (SSP5-8.5). The dataset includes monthly and daily temperature, precipitation, and other variables, requiring storage of 275 TB. Additionally, the surface air temperature (SAT) and land precipitation simulated by the FGOALS-g3 super-large ensemble have been validated and projected. The ensemble can capture the response of SAT and land precipitation to external forcings well, and the internal variabilities can be quantified. The availability of more than 100 realizations will help researchers to study rare events and improve the understanding of the impact of internal variability on forced climate changes.

Three-dimensional characteristics of mesoscale eddies simulated by a regional model in the northwestern Pacific Ocean during 2000–2008 - Guijing Yang; Xiayan Lin; Guoqing Han; Yu Liu; Gengxin Chen; Jianhui Wang (Acta Oceanologica Sinica, 2022)

Horizontal and vertical heterogeneity of sediment microbial community in Site F cold seep, the South China Sea - Xinyi Zhai; Xiaochong Shi; Haojin Cheng; Peng Yao; Bin Zhao; Lin Chen; Jiwen Liu; Lei Cao; Minxiao Wang; Lulu Fu; Xiao-Hua Zhang; Min Yu (Frontiers in Marine Science, 2022)
Site F is the most vigorous cold seep known on the continental slope of the northern South China Sea. Up to now, the microbial community structures in sediments of Site F based on the high-throughput sequencing of the 16S rRNA genes have been studied extensively. However, few studies investigated the microbial community structures at fine vertical scales of Site F and control stations outside Site F. In this study, a comprehensive investigation of microbial communities in sediments of Site F along the depths varying from 0 to 24 cm below sea floor (cmbsf) of four sampling sites—SRS (Southern Reduced Sediment), NRS (Northern Reduced Sediment), Control 1 (close to Site F), and Control 2 (far from Site F)—was carried out. The high relative abundances of anaerobic methanotrophic archaea (ANME), Desulfobacterota [sulfate-reducing bacteria (SRB)], and Campylobacteria [sulfur-oxidizing bacteria (SOB)] in SRS and NRS indicated that these two sites were newborn cold seep sites compared with non-seep sites, Control 1, and Control 2. A positive correlation between ANME-1b, ANME-2, and SEEP-SRB and an enrichment of Sulfurovum and Methlomonadaceae were found in the surface sediments of both SRS and NRS, indicating that the processes of anaerobic oxidation of methane (AOM), sulfur oxidation, and sulfate reduction might occur in seep sites. SRS was enriched with ANME-1b and SEEP-SRB2 with a proposed sulfate-methane transition zone (SMTZ) approximately located at 8 cmbsf. The high abundance of ANME in SRS may due to the high concentration of methane. NRS was enriched with ANME-2, Desulfatiglans , Sulfurovum , and Methanosarcinaceae with a proposed SMTZ at about 10 cmbsf. According to the analyses of microbial community structure and environmental factors, NRS could be described as a notable cold seep reduced sediment site with low sulfate and high H 2 S that nourished abundant SEEP-SRB1, ANME-2, Methanosarcinales , and Sulfurovum , which showed similar distribution pattern. Our study expands the current knowledge on the differences of microbial communities in cold seep sites and non-seep sites and sheds light on the horizontal and vertical heterogeneity of sediment microbial community in Site F.

Shallow-water hydrothermal vent system as an extreme proxy for discovery of microbiome significance in a crustacean holobiont - Ling Chiu; Min-Chen Wang; Kuang-Yu Tseng; Chih-Lin Wei; Huei-Ting Lin; Shan-Hua Yang; Yung-Che Tseng (Frontiers in Marine Science, 2022)
The shallow-water hydrothermal vent (HV) system off Kueishan Island lies at the end of the Okinawa Trough to the northeast of Taiwan. Near its submarine vent openings, aperiodic vent discharges generate a dynamic acidic (pH 5.5-8.1) and sulfidic (9-3000 μM) ecosystem. The dominant metazoan in this unique environment is the brachyuran vent crab, Xenograpsus testudinatus , which has developed robust metabolic strategies and highly adaptive acid-base regulatory mechanisms to maintain its physiological homeostasis. X. testudinatus is considered a holobiont, but the symbiotic mechanisms underlying acid and sulfur tolerance in the host-microbe system remain largely unclear. In this study, we used LoopSeq long-read sequencing of the full-length 16S rRNA gene to identify the bacterial communities present in the gills and carapace surface of X. testudinatus . The alpha diversity analysis, Venn diagram, and principal coordinate analysis (PCoA) indicated that the gills and carapace surface exhibit different bacterial constituents. Further measurements of relative abundance, coupled with functional predictions and fluorescence in situ hybridization (FISH), revealed a predominance of Sulfurovum sp. NBC37-1, a key bacterium that can perform sulfur and hydrogen oxidation to support denitrification processes. Consequently, our findings suggest that the symbiotic bacteria may play a critical role in conferring the extraordinary acid and sulfur tolerances of X. testudinatus , allowing the crustacean holobiont to thrive in its ecological niche within one of the most extreme marine habitats on Earth.

Altered Retinal Dopamine Levels in a Melatonin-proficient Mouse Model of Form-deprivation Myopia - Kang-Wei Qian; Yun-Yun Li; Xiao-Hua Wu; Xue Gong; Ai-Lin Liu; Wen-Hao Chen; Zhe Yang; Ling-Jie Cui; Yun-Feng Liu; Yuan-Yuan Ma; Chen-Xi Yu; Furong Huang; et al. (Neuroscience Bulletin, 2022)

Validity Analysis of the Morphological Identification of Three Highly Morphologically Similar Species Belonging to the Genus Niviventer (Rodentia: Muridae) - Yao-Yao Li; Hao-Tian Li; Jia-Wei Hao; Chen-Yi Zhang; Guan-Ze Liu; Xing-Han Lin; Xiao-Qing Sun; Guo-Chen Zang; Yu-Chun Li (Journal of Mammalian Evolution, 2022)

ZAKβ Alleviates Oxidized Low-density Lipoprotein (ox-LDL)-Induced Apoptosis and B-type Natriuretic Peptide (BNP) Upregulation in Cardiomyoblast - Yueh-Min Lin; Jiro Hasegawa Situmorang; Jia-Zun Guan; Dennis Jine-Yuan Hsieh; Jaw-Ji Yang; Michael Yu-Chih Chen; Ching-Hui Loh; Chia-Hua Kuo; Shang-Yeh Lu; Ying-Ming Liou; Chih-Yang Huang (Cell Biochemistry and Biophysics, 2022)

Effect of confining pressure on the fracture mechanical properties of multi-particle sandstone - Kai-wen Shi; Yu-jun Zuo; Wenjibin Sun; Hao Liu; Chao Pan; Taotao Li; Bin Chen; Jian-yun Lin (Arabian Journal of Geosciences, 2022)

Climatology of aerosol types and their vertical distribution over East Asia based on CALIPSO lidar measurements - Lu Gui; Minghui Tao; Yi Wang; Lunche Wang; Liangfu Chen; Changqing Lin; Jinhua Tao; Jun Wang; Chao Yu (International Journal of Climatology, 2022)
Aerosol vertical distribution generally determines their health impacts and climate effects. By using long‐term (2007–2019) CALIPSO lidar measurements, we present a large‐scale insight into the climatology of aerosol types and their vertical structure over East Asia. Despite the low sampling frequency, comparison with MODIS aerosol optical depth (AOD) shows that the integrated CAIPSO vertical extinction can reasonably reproduce spatial patterns of the aerosol loading. With the unique advantage of active detection, CALIPSO reveals an obvious enhancement of the night‐time AOD due to worse diffusion conditions. Moreover, long‐range transport of different aerosols including dust, polluted dust, and smoke has substantial contribution to the aerosol loading over East Asia. Pure dust particles are mainly concentrated over the deserts with notable dust transport belts (dust AOD > 0.2 at 532 nm) along downwind regions during winter and spring. By contrast, polluted dust is prevalent over the downstream eastern China with much higher AODs throughout the year. In particular, AOD of polluted dust (~0.5) is higher in winter rather than in spring, which is consistent with their seasonal occurrences. Smoke aerosols usually appear in the night‐time over southern China. The top heights of aerosols are around 4–6 km, with dust and smoke having higher extinction at upstream regions. The climatology of these aerosol types and vertical distribution can provide a significant constraint for associated studies of air quality and climate effects.

Age, Growth and Population Structure Analyses of the Purpleback Flying Squid Sthenoteuthis oualaniensis in the Northwest Indian Ocean by Beak Microstructure - Hua-Jie Lu; Yu-Zhe Ou; Jing-Ru He; Mao-Lin Zhao; Zi-Yue Chen; Xin-Jun Chen (Journal of Marine Science and Engineering, 2022)
To explore the feasibility of using beak microstructure information to estimate the age of Sthenoteuthis oualaniensis, the microstructures of the upper beaks of individual squid were applied in this work to analyze the ages and growth patterns of squid caught from February–May 2019 and from October–December 2020 in the northwest Indian Ocean. The results indicated that the squid samples in the two years were no older than 9 months, and the samples in 2019 were autumn population and 2020 were spring population. The linear growth model of the autumn population (2019) was the best model for describing the relationship between age and ML, while the power model of the spring population (2020) was the best for describing the relationship between age and ML. The maximum instantaneous growth rate (IGR) and absolute daily growth rate (AGR) values of the spring population were 0.24%/d and 1.09 mm/d, respectively, occurring in squid between 200 and 220 days of age. The maximum IGR and AGR values of the autumn population were 0.69%/d and 1.73 mm/d, respectively, occurring in squid between 200 and 240 days of age. The period from 141–260 days (5–8 months) was considered to correspond to the subadult stage in the whole life history of S. oualaniensis in the Northwest Indian Ocean. The beak microstructure information can be effectively applied to estimate the age of S. oualaniensis individuals.

Natural variation of the BRD2 allele affects plant height and grain size in rice - Jinpeng Huang; Zhiming Chen; Jiajia Lin; Jinwen Chen; Menghao Wei; Liang Liu; Feng Yu; Zesen Zhang; Fangyu Chen; Liangrong Jiang; Jingsheng Zheng; Tiansheng Wang; et al. (Planta, 2022)

Biomechanical Analysis of Mandibular Implant-Assisted Removable Partial Denture with Distal Extension - Yung-Chung Chen; Chi-Lun Lin; Chen-Han Yu; Hung-Chih Chang; Yang-Ming Lin; Jia-Wei Lin (Journal of Medical and Biological Engineering, 2022)

Differential responses of bacterial communities in coral tissue and mucus to bleaching - Yiyang Zou; Yu Chen; Lin Wang; Si Zhang; Jie Li (Coral Reefs, 2022)

Deep learning based diagnosis of Parkinson’s Disease using diffusion magnetic resonance imaging - Hengling Zhao; Chih-Chien Tsai; Mingyi Zhou; Yipeng Liu; Yao-Liang Chen; Fan Huang; Yu-Chun Lin; Jiun-Jie Wang (Brain Imaging and Behavior, 2022)

Fissistiganoids A and B: two new flavonoids from the Fissistigma tungfangense - Wan-Lin Zhong; Xue-Ming Zhou; Ji-Ling Yi; Xin-Ming Song; Bin Zhang; Jing-Yu Yang; Guang-Ying Chen (Natural Product Research, 2022)

High-Throughput Identification of Antihypertensive Peptides (AHTPs) and Characterization of AHTP-Derived Genes in the Lined Seahorse (Hippocampus erectus) - Yu Huang; Xiyang Chen; Haoyue Shu; Panpan Xiao; Xueqiang Lin; Junmin Xu; Chao Bian; Xinxin You; Jian Yang; Qiong Shi (Frontiers in Marine Science, 2022)
Recently, the prevalence of hypertension has become a global challenge. Therefore, tremendous efforts have been made to identify and purify antihypertensive peptides (AHTPs) from food-derived proteins to aid the discovery of new hypotensive drugs with fewer side effects. In this study, we performed high-throughput prediction of AHTPs based on multi-omics data, providing an overview of AHTPs in the lined seahorse proteins and suggesting their potential application as bioactive agents to lower blood pressure. We identified 14,695 AHTP-derived genes in the lined seahorse, and most of them were supported by transcriptomic evidence, whereas only 495 genes were further detected by proteome sequencing. Among these predicted AHTP-derived genes, the longest titin had the most hits with 104 AHTPs, some of which were clustered in exon 158, 194, and 204. Another AHTP-rich group was in the collagen family, and four AHTP-rich collagens exhibited much higher transcription in the pouch than in other examined tissues, including brain, testis, and embryos. Additionally, antihypertensive triplets, comprised of the permutations of Gly, Pro, and Lys, prevailed in all collagen sequences due to the representative XaaYaaGly repeat units. In summary, our present findings provide a solid basis for understanding the abundance of various AHTPs in the lined seahorse as well as shed light on the development of antihypertensive products and drugs using seahorses as an important resource.

Development of wheat-Dasypyrum villosum T6V#4S·6AL translocation lines with enhanced inheritance for powdery mildew resistance - Xiaolan Ma; Yanan Chang; Jingnan Chen; Mei Yu; Baicui Wang; Xingguo Ye; Zhishan Lin (Theoretical and Applied Genetics, 2022)

Metatranscriptomic analysis of host response and vaginal microbiome of patients with severe COVID-19 - Meng Xiao; Bo Lu; Rui Ding; Xia Liu; Xian Wu; Yaqian Li; Xudong Liu; Lin Qiu; Zhibo Zhang; Jing Xie; Yu Chen; Dong Zhang; et al. (Science China Life Sciences, 2022)

Cross-generation trophic ecology of Dosidicus gigas in the eastern-central Pacific Ocean revealed from isotopic signatures in eye lenses - Guan Yu Hu; Zi Xin Wang; Bi Lin Liu; Meng Yao Huan; Xin Jun Chen (Fisheries Science, 2022)

Characterization, risk assessment and resource potential of sediments in the black-odor water in Hunan, China - Jingwei Ma; Xiao Lin; Zefang Yu; Xiang Wang; Jiyi Chen; Qiulai He (Environmental Monitoring and Assessment, 2022)

Dapagliflozin protects against doxorubicin-induced cardiotoxicity by restoring STAT3 - Wei-Ting Chang; Jhih-Yuan Shih; Yu-Wen Lin; Zhih-Cherng Chen; Wei-Chih Kan; Tsung-Hsien Lin; Chon-Seng Hong (Archives of Toxicology, 2022)

Streptococcus vulneris sp. nov., isolated from wound of patient with diabetic foot ulcer (DFU) - Jen-hao Chung; Li-Ting Wang; Yi-Sheng Chen; Yun-Shien Lee; Shih-Ting Lin; Yu-Chung Chang; Hui-Chung Wu; Yu-Jie Dai; Wei-Qi Liao; Fan-Ching Kao; Jui-hsiang Li (Archives of Microbiology, 2022)

Investment Feasibility Analysis of Large Submersible Cage Culture in Taiwan: A Case Study of Snubnose Pompano (Trachinotus anak) and Cobia (Rachycentron canadum) - Hsun-Yu Lan; Farok Afero; Cheng-Ting Huang; Bo-Ying Chen; Po-Lin Huang; Yen-Lung Hou (Fishes, 2022)
Onshore farming for premium aquaculture is under scrutiny and criticism partially due to possible causes of adverse environmental impacts on other resource users and the surrounding environment. The best alternative to preventing or minimizing these impacts is to utilize open seawater by large submersible cage culture. The current operation in Taiwan has demonstrated that the culture operation is technically feasible but economically demanding because of high capital and operating costs. Therefore, this study conducted an economic analysis of the expansion of large submersible cage culture by selecting two premium species of snubnose pompano (Trachinotus anak) and cobia (Rachycentron canadum) and examined the profitability of large submersible cage culture investment. This study found that the current operation of four-unit cages highlighted a negative net present value and internal rate of return with a payback period of over ten and six years, respectively. Large submersible cage culture can be financially profitable when its operation unit expands from 8 to 24 units. Increasing unit cages to eight incurred a gross margin of 17.09%, BCR 1.21, with a payback period of 5.36 years. Expanding the operation to 24-unit cages was a potentially lucrative investment with a gross margin of 18.51%, BCR 1.23, PI 2.15, internal rate of return of 20.84%, and a payback period of 3.55 years. Sensitivity analyses revealed that market price and survival rate significantly impact the profitability of large submersible cage culture. Finally, it is suggested that producers could invest in 8-unit cages and maintaining the survival rate of snubnose pompano and cobia at 80% and 40%, respectively.

Fine mapping qKRN5.04 provides a functional gene negatively regulating maize kernel row number - Yixin An; Lin Chen; Yong-Xiang Li; Chunhui Li; Yunsu Shi; Dengfeng Zhang; Yu Li; Tianyu Wang (Theoretical and Applied Genetics, 2022)

Identification of the Largest SCA36 Pedigree in Asia: with Multimodel Neuroimaging Evaluation for the First Time - Yue Xie; Zhao Chen; Zhe Long; Rui-Ting Chen; Yi-Zheng Jiang; Ming-Jie Liu; Lin-Liu Peng; Hui-Rong Peng; Na Wan; Guangdong Zou; Chun-Rong Wang; Yu-Ting Shi; et al. (The Cerebellum, 2022)

Lactobacillus plantarum PS128 Promotes Intestinal Motility, Mucin Production, and Serotonin Signaling in Mice - Chih-Ming Chen; Chien-Chen Wu; Chin-Lin Huang; Min-Yu Chang; Shih-Hsuan Cheng; Ching-Ting Lin; Ying-Chieh Tsai (Probiotics and Antimicrobial Proteins, 2022)
Lactobacillus plantarum PS128 has been reported as a psychobiotic to improve mental health through the gut–brain axis in experimental animal models. To explore its mechanism of action in the gut, this study aimed to analyze the effects of L. plantarum PS128 ingestion on naïve and loperamide (Lop)-induced constipation mice. We found that, in the two mouse models, the weight, number, and water content of feces in the L. plantarum PS128 group were higher than those in the vehicle control group. Histological observation revealed that L. plantarum PS128 increased the level of colonic mucins including the major mucin MUC2. In addition, the charcoal meal test showed that L. plantarum PS128 significantly increased the small intestine transit in naïve mice, but not in the Lop-treated mice. Since intestinal serotonin has been found to modulate motility, we further analyzed the expression of genes related to serotonin signal transduction in the small intestine of naïve mice. The results showed that L. plantarum PS128 significantly altered the expression levels of Tph1 , Chga , Slc6a4 , and Htr4 , but did not affect the expression levels of Tph2 , Htr3a , and Maoa . Furthermore, immunohistochemistry revealed that L. plantarum PS128 significantly increased the number of serotonin-containing intestinal cells in mice. Taken together, our results suggest that L. plantarum PS128 could promote intestinal motility, mucin production, and serotonin signal transduction, leading to a laxative effect in mice.

A Deep Mesencephalic Nucleus Circuit Regulates Licking Behavior - Di Zheng; Jia-Yu Fu; Meng-Yu Tang; Xiao-Dan Yu; Yi Zhu; Chen-Jie Shen; Chun-Yue Li; Shi-Ze Xie; Shan Lin; Minmin Luo; Xiao-Ming Li (Neuroscience Bulletin, 2022)

Molecular cloning, characterization and expression analysis of two 12-oxophytodienoate reductases (NtOPR1 and NtOPR2) from Nicotiana tabacum - Feiyan Huang; Farhat Abbas; Dylan O’Neill Rothenberg; Muhammad Imran; Sajid Fiaz; Naveed ur Rehman; Sikandar Amanullah; Afifa Younas; Yan Ding; Xianjie Cai; Xiaolong Chen; Lei Yu; et al. (Molecular Biology Reports, 2022)

Use of piggyBac Transposon System Constructed Murine Breast Cancer Model for Reporter Gene Imaging and Characterization of Metastatic Tumor Cells - Ying-Ling Chen; Yung-Cheng Wang; Kuei-Yuan Hou; Min-Ying Lin; Yu-Chuan Lin; Hui-Yen Chuang; Yi-Jang Lee (Journal of Medical and Biological Engineering, 2022)

Astragalus Polysaccharides Reduce High-glucose-induced Rat Aortic Endothelial Cell Senescence and Inflammasome Activation by Modulating the Mitochondrial Na+/Ca2+ Exchanger - Xin-Yu Miao; Xiao-Xiao Zhu; Zhao-Yan Gu; Bo Fu; Shao-Yuan Cui; Yuan Zu; Ling-Jun Rong; Fan Hu; Xiang-Mei Chen; Yan-Ping Gong; Chun-Lin Li (Cell Biochemistry and Biophysics, 2022)

Identification of Key Pathways and Genes in SARS-CoV-2 Infecting Human Intestines by Bioinformatics Analysis - Ji-Chun Chen; Tian-Ao Xie; Zhen-Zong Lin; Yi-Qing Li; Yu-Fei Xie; Zhong-Wei Li; Xu-Guang Guo (Biochemical Genetics, 2022)
COVID-19 is a serious infectious disease that has recently swept the world, and research on its causative virus, SARS-CoV-2, remains insufficient. Therefore, this study uses bioinformatics analysis techniques to explore the human digestive tract diseases that may be caused by SARS-CoV-2 infection. The gene expression profile data set, numbered GSE149312, is from the Gene Expression Omnibus (GEO) database and is divided into a 24-h group and a 60-h group. R software is used to analyze and screen out differentially expressed genes (DEGs) and then gene ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses are performed. In KEGG, the pathway of non-alcoholic fatty liver disease exists in both the 24-h group and 60-h group. STRING is used to establish a protein–protein interaction (PPI) network, and Cytoscape is then used to visualize the PPI and define the top 12 genes of the node as the hub genes. Through verification, nine statistically significant hub genes are identified: AKT1, TIMP1, NOTCH, CCNA2, RRM2, TTK, BUB1B, KIF20A, and PLK1. In conclusion, the results of this study can provide a certain direction and basis for follow-up studies of SARS-CoV-2 infection of the human digestive tract and provide new insights for the prevention and treatment of diseases caused by SARS-CoV-2.

Three-dimensional modeling and porosity calculation of coal rock pore structure - Qiong Li; Zheng Chen; Lin Yue; Yu Zhang; Jian-Jun He; Yong Li (Applied Geophysics, 2022)

Distinct Evolution of the SST Anomalies in the Far Eastern Pacific between the 1997/98 and 2015/16 Extreme El Niños - Shaolei Tang; Jing-Jia Luo; Lin Chen; Yongqiang Yu (Advances in Atmospheric Sciences, 2022)

Natural Feed Supplements Improve Growth, Non-Specific Immune Responses and Resistance against Vibrio alginolyticus in Lates calcarifer - Zhen-Hao Liao; Huai-Ting Huang; Yu-Ru Lin; Bo-Ying Chen; Yi-Fan Lee; Yuan-Heng Lin; Chieh-Yuan Chuang; Fan-Hua Nan (Journal of Marine Science and Engineering, 2022)
This study aimed to investigate the effects of dietary natural feed supplement on the growth performance, non-specific responses, and disease resistance in Lates calcarifer. Three commercial products (A, B, and C) containing a basal probiotic mixture were tested. Product A contained a basal mixture of Paenibacillus sp., Bacillus subtilis, Bacillus amyloliquefaciens, and Lactobacillus rhamnosus (107 cfu/g for each probiotic); product B contained additional Lactobacillus plantarum (1010 cfu/g); and product C contained additional soybean peptides (500 g/kg) and garlic powder (1 g/kg). Each product was supplemented into subject diets at dosages of 1 or 2 g/kg (designated as the A1, A2, B1, B2, C1, and C2 groups, respectively). Following an eight-week trial, growth parameters (specific growth rate and feed conversion ratio), non-specific immune responses (O2− production, phagocytic rate, and phagocytic index), and the results of a challenge test against Vibrio alginolyticus were evaluated. The results show that all probiotic supplement groups exhibited an improvement in growth performance compared to the control group (non-probiotic diet). In terms of non-specific immunity parameters, a significant improvement in O2− production was found in the C2 group, whereas significant improvements in phagocytic activity were found in all the B and C groups. The C2 group displayed optimal O2− production, phagocytic rate, and phagocytic index results. For the challenge test, the C groups showed higher Vibrio resistance than the other experimental groups and the control group. These results suggest that product C, given at dosages of 2 g/kg, may serve as a growth-promoting and immunostimulatory additive for the cultivation of Asian seabass.

Shear Instability and Turbulent Mixing in the Stratified Shear Flow Behind a Topographic Ridge at High Reynolds Number - Jia-Lin Chen; Xiao Yu; Ming-Huei Chang; Sen Jan; Yiing Jang Yang; Ren-Chieh Lien (Frontiers in Marine Science, 2022)
Observations on the lee of a topographic ridge show that the turbulence kinetic energy (TKE) dissipation rate due to shear instabilities is three orders of magnitude higher than the typical value in the open ocean. Laboratory-scale studies at low Reynolds number suggest that high turbulent dissipation occurs primarily within the core region of shear instabilities. However, field-scale studies indicate that high turbulence is mainly populated along the braids of shear instabilities. In this study, a high-resolution, resolving the Ozmidov-scale, non-hydrostatic model with Large Eddy Simulation (LES) turbulent closure is applied to investigate dominant mechanisms that control the spatial and temporal scales of shear instabilities and resulting mixing in stratified shear flow at high Reynolds number. The simulated density variance dissipation rate is elevated in the cusp-like bands of shear instabilities with a specific period, consistent with the acoustic backscatter taken by shipboard echo sounder. The vertical length scale of each cusp-like band is nearly half of the vertical length scale of the internal lee wave. However, it is consistent with instabilities originating from a shear layer based on linear stability theory. The model results indicate that the length scale and/or the period of shear instabilities are the key parameters to the mixing enhancement that increases with lateral Froude number Fr L , i.e. stronger shear and/or steeper ridge.

Metabolic tuning of a stable microbial community in the surface oligotrophic Indian Ocean revealed by integrated meta-omics - Zhang-Xian Xie; Ke-Qiang Yan; Ling-Fen Kong; Ying-Bao Gai; Tao Jin; Yan-Bin He; Ya-Yu Wang; Feng Chen; Lin Lin; Zhi-Long Lin; Hong-Kai Xu; Zong-Ze Shao; et al. (Marine Life Science & Technology, 2022)
Understanding the mechanisms, structuring microbial communities in oligotrophic ocean surface waters remains a major ecological endeavor. Functional redundancy and metabolic tuning are two mechanisms that have been proposed to shape microbial response to environmental forcing. However, little is known about their roles in the oligotrophic surface ocean due to less integrative characterization of community taxonomy and function. Here, we applied an integrated meta-omics-based approach, from genes to proteins, to investigate the microbial community of the oligotrophic northern Indian Ocean. Insignificant spatial variabilities of both genomic and proteomic compositions indicated a stable microbial community that was dominated by Prochlorococcus , Synechococcus , and SAR11. However, fine tuning of some metabolic functions that are mainly driven by salinity and temperature was observed. Intriguingly, a tuning divergence occurred between metabolic potential and activity in response to different environmental perturbations. Our results indicate that metabolic tuning is an important mechanism for sustaining the stability of microbial communities in oligotrophic oceans. In addition, integrated meta-omics provides a powerful tool to comprehensively understand microbial behavior and function in the ocean.

Identification of PDXDC1 as a novel pleiotropic susceptibility locus shared between lumbar spine bone mineral density and birth weight - Yu-Qian Song; Shi-Di Hu; Xu Lin; Xiang-He Meng; Xiao Wang; Yin-Hua Zhang; Cheng Peng; Rui Gong; Tao Xu; Tong Zhang; Chen-Zhong Li; Dao-Yan Pan; et al. (Journal of Molecular Medicine, 2022)
An increasing number of epidemiological studies have suggested that birth weight (BW) may be a determinant of bone health later in life, although the underlying genetic mechanism remains unclear. Here, we applied a pleiotropic conditional false discovery rate (cFDR) approach to the genome-wide association study (GWAS) summary statistics for lumbar spine bone mineral density (LS BMD) and BW, aiming to identify novel susceptibility variants shared between these two traits. We detected 5 novel potential pleiotropic loci which are located at or near 7 different genes ( NTAN1 , PDXDC1 , CACNA1G , JAG1 , FAT1P1 , CCDC170 , ESR1 ), among which PDXDC1 and FAT1P1 have not previously been linked to these phenotypes. To partially validate the findings, we demonstrated that the expression of PDXDC1 was dramatically reduced in ovariectomized (OVX) mice in comparison with sham-operated (SHAM) mice in both the growth plate and trabecula bone. Furthermore, immunohistochemistry assay with serial sections showed that both osteoclasts and osteoblasts express PDXDC1 , supporting its potential role in bone metabolism. In conclusion, our study provides insights into some shared genetic mechanisms for BMD and BW as well as a novel potential therapeutic target for the prevention of OP in the early stages of the disease development. Key messages We investigated pleiotropy-informed enrichment between LS BMD and BW. We identified genetic variants related to both LS BMD and BW by utilizing a cFDR approach. PDXDC1 is a novel pleiotropic gene which may be related to both LS BMD and BW. Elevated expression of PDXDC1 is related to higher BMD and lower ratio n-6/n-3 PUFA indicating a bone protective effect of PDXDC1.

Bioeconomic evaluation of Eleutheronema tetradactylum farming: a case study in Taiwan - Cheng-Ting Huang; Farok Afero; Fang-Yu Lu; Bo-Ying Chen; Po-Lin Huang; Hsun-Yu Lan; Yen-Lung Hou (Fisheries Science, 2022)

Epidemiological characteristics of gonorrhea and its influential meteorological factors: a 14-year retrospective assessment in China - Jin Bu; Liang-Jia Zhou; Xiang Xiao; Qian Yue; Gang Hu; Pei Jiang; Jing Chen; Yu Lin (Environmental Science and Pollution Research, 2022)

Short-term variations and influencing factors of suspended sediment concentrations at the Heisha Beach, Guangdong, China - Jintang Ou; Haoyan Dong; Liangwen Jia; Xiangxin Luo; Zixiao He; Kanglin Chen; Jing Liu; Yitong Lin; Mingdong Yu; Mingen Liang (Acta Oceanologica Sinica, 2022)

Evolutionary and ecological patterns of scatter‐ and larder‐hoarding behaviours in rodents - Yongjun Zhang; Fei Yu; Xianfeng Yi; Weiwei Zhou; Rui Liu; Marcel Holyoak; Lin Cao; Mingming Zhang; Jiani Chen; Zhibin Zhang; Chuan Yan (Ecology Letters, 2022)
Scatter‐ and larder hoarding are the primary strategies of food‐hoarding animals and have important implications for plant‐animal interactions and plant recruitment. However, their origins and influencing factors have not been fully investigated across a wide range of taxa. Our systematic literature search amassed data for 183 seed‐hoarding rodent species worldwide and tested relationships of seed‐hoarding behaviours with phylogenetic signal, functional traits and environmental factors. We found that the evolution of hoarding strategies was not random in phylogeny, and scatter hoarding originated independently multiple times from larder hoarding. Rodents with higher encephalisation quotient (relative brain size), omnivorous diet (related to dependence on seeds) and inhabiting lower latitudes were disproportionately likely to scatter hoard. Despite body mass’s potential relationship with competition through food defence, it was associated with food‐hoarding strategy only in a few families. Our results show the need to study the community and ecological context of food‐hoarding behaviours.

Influence of the spatial distribution pattern of buildings on the distribution of PM2.5 concentration - Zhixuan Zhang; Baoyan Shan; Qikai Lin; Yanqiu Chen; Xinwei Yu (Stochastic Environmental Research and Risk Assessment, 2022)

Dissecting the Neural Circuitry for Pain Modulation and Chronic Pain: Insights from Optogenetics - Fang Guo; Yu Du; Feng-Hui Qu; Shi-Da Lin; Zhong Chen; Shi-Hong Zhang (Neuroscience Bulletin, 2022)
Pain is an unpleasant sensory and emotional experience associated with, or resembling that associated with, actual or potential tissue damage. The processing of pain involves complicated modulation at the levels of the periphery, spinal cord, and brain. The pathogenesis of chronic pain is still not fully understood, which makes the clinical treatment challenging. Optogenetics, which combines optical and genetic technologies, can precisely intervene in the activity of specific groups of neurons and elements of the related circuits. Taking advantage of optogenetics, researchers have achieved a body of new findings that shed light on the cellular and circuit mechanisms of pain transmission, pain modulation, and chronic pain both in the periphery and the central nervous system. In this review, we summarize recent findings in pain research using optogenetic approaches and discuss their significance in understanding the pathogenesis of chronic pain.

TRIP13 knockdown inhibits the proliferation, migration, invasion, and promotes apoptosis by suppressing PI3K/AKT signaling pathway in U2OS cells - De-Chen Yu; Xiang-Yi Chen; Hai-Yu Zhou; De-Quan Yu; Xiao-Lei Yu; Yi-Cun Hu; Rui-Hao Zhang; Xiao-Bo Zhang; Kun Zhang; Mao-Qiang Lin; Xi-Dan Gao; Tao-Wen Guo (Molecular Biology Reports, 2022)

Genetic diversity and population structure of Curcuma alismatifolia Gagnep. accessions revealed by SSR markers - Hui-Wen Yu; Ling-Jun Ke; Yi-Lan Xiao; Si-Jia Chen; Yuan-Yuan Li; Qi-Lin Tian; Yun-He Li; Jin-Shui Lin; Luan-Mei Lu (Genetic Resources and Crop Evolution, 2022)

Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae - Xin-Yi Tao; Yang-Chen Lin; Feng-Qing Wang; Qing-Hai Liu; Yu-Shu Ma; Min Liu; Dong-Zhi Wei (Biotechnology Letters, 2022)

Selenium Alleviates Inflammation in Staphylococcus aureus-Induced Mastitis via MerTK-Dependent Activation of the PI3K/Akt/mTOR Pathway in Mice - Si-jie Chen; Chen-yuan Zhang; Di Yu; Chang-jie Lin; Hao-jun Xu; Chang-min Hu (Biological Trace Element Research, 2022)

Haloprofundus salilacus sp. nov., Haloprofundus halobius sp. nov. and Haloprofundus salinisoli sp. nov.: three extremely halophilic archaea isolated from salt lake and saline soil - Si-Ya Li; Yu-Jie Xin; Chen-Xi Bao; Jing Hou; Heng-Lin Cui (Extremophiles, 2022)

Evaluating the probiotic potential and adhesion characteristics of Bacillus spp. isolated from the intestine of Rhynchocypris lagowskii Dybowski - Yurou Zhang; Zhenchao Wu; Mengnan Yu; Dongming Zhang; Qiuju Wang; Lili Lin; Guiqin Wang; Mahmoud M. Elsadek; Qi Yao; Yuke Chen; Zhixin Guo (Aquaculture International, 2022)

Extensive sequence divergence between the reference genomes of Taraxacum kok-saghyz and Taraxacum mongolicum - Tao Lin; Xia Xu; Huilong Du; Xiuli Fan; Qingwen Chen; Chunyan Hai; Zijian Zhou; Xiao Su; Liquan Kou; Qiang Gao; Lingwei Deng; Jinsheng Jiang; et al. (Science China Life Sciences, 2022)

Transcriptomic analyses provide insight into adventitious root formation of Euryodendron excelsum H. T. Chang during ex vitro rooting - Yuping Xiong; Shuangyan Chen; Zhenpeng Wei; Xiaohong Chen; Beiyi Guo; Ting Zhang; Yuying Yin; Xincheng Yu; Jinhui Pang; Meiyun Niu; Xinhua Zhang; Yuan Li; et al. (Plant Cell, Tissue and Organ Culture (PCTOC), 2022)

Fate of heavy metals and bacterial community composition following biogas slurry application in a single rice cropping system - Qiang Wang; Zhaoming Chen; Jun Zhao; Junwei Ma; Qiaogang Yu; Ping Zou; Hui Lin; Jinchuan Ma (Journal of Soils and Sediments, 2022)

Establishment of Galectin-3 Time-resolved Fluoroimmunoassay and its Application in Idiopathic Membranous Nephropathy - Xiaomei Yu; Lingli Chen; Bo Lin; Li Zhang; Xue Yang; Xiaobin Liu; Pengguo Xia; Yueming Liu; Shaoxiong Zheng; Xiumei Zhou; Yigang Wang; Yuan Qin; et al. (Journal of Fluorescence, 2022)

The effect of preoperative statin treatment on acute kidney injury in elderly patients undergoing valve replacement surgery - Bing-qi Fu; Xue-biao Wei; Zedazhong Su; Ying-wen Lin; Zu-hui Ke; Tong Tan; Ji-yan Chen; Shou-hong Wang; Dan-qing Yu (European Journal of Clinical Pharmacology, 2022)

Ag3PO4-based photocatalysts and their application in organic-polluted wastewater treatment - Chunmu Yu; Xiaojuan Chen; Ning Li; Yue Zhang; Sailin Li; Jieming Chen; Liang Yao; Kaichun Lin; Yiqi Lai; Xinru Deng (Environmental Science and Pollution Research, 2022)

Application of Soil and Water Assessment Tool (SWAT) to evaluate the fates of nitrogenous fertilizer in subtropical mountainous watershed tea farms - Guan-Zhou Lin; Shao-Yiu Hsu; Chia-Chun Ho; Chi-Feng Chen; Jr-Chuan Huang; Tsung-Yu Lee (Environmental Monitoring and Assessment, 2022)

Enhancement of oral bioavailability and anti-hyperuricemic activity of aloe emodin via novel Soluplus®—glycyrrhizic acid mixed micelle system - Feng Shi; Lin Chen; Yaping Wang; Jing Liu; Michael Adu-Frimpong; Hao Ji; Elmurat Toreniyazov; Qilong Wang; Jiangnan Yu; Ximing Xu (Drug Delivery and Translational Research, 2022)

Early-Middle Permian carbon-isotope stratigraphy of marine carbonates in the northern edge of the South China: implications for global correlation - Qian Li; Karem Azmy; Shuai Yang; Hongde Chen; Shenglin Xu; Liangbiao Lin; Zhongtang Su; Anqing Chen; Yu Yu; Fengrui Sun (Carbonates and Evaporites, 2022)

Description of Aureibaculum luteum sp. nov. and Aureibaculum flavum sp. nov. isolated from Antarctic intertidal sediments - Xiao-yan He; Ning-hua Liu; Chao-yi Lin; Mei-ling Sun; Xiu-lan Chen; Yu-zhong Zhang; Yu-qiang Zhang; Xi-ying Zhang (Antonie van Leeuwenhoek, 2022)

Prevalence, complete genome, and metabolic potentials of a phylogenetically novel cyanobacterial symbiont in the coral‐killing sponge, Terpios hoshinota - Yu‐Hsiang Chen; Hsing‐Ju Chen; Cheng‐Yu Yang; Jia‐Ho Shiu; Daphne Z. Hoh; Pei‐Wen Chiang; Wenhua Savanna Chow; Chaolun Allen Chen; Tin‐Han Shih; Szu‐Hsien Lin; Chi‐Ming Yang; James Davis Reimer; et al. (Environmental Microbiology, 2022)
Summary Terpios hoshinota is an aggressive, space‐competing sponge that kills various stony corals. Outbreaks of this species have led to intense damage to coral reefs in many locations. Here, the first large‐scale 16S rRNA gene survey across three oceans revealed that bacteria related to the taxa Prochloron , Endozoicomonas , SAR116, Ruegeria , and unclassified Proteobacteria were prevalent in T . hoshinota . A Prochloron ‐related bacterium was the most dominant and prevalent cyanobacterium in T . hoshinota . The complete genome of this uncultivated cyanobacterium and pigment analysis demonstrated that it has phycobiliproteins and lacks chlorophyll b , which is inconsistent with the definition of Prochloron . Furthermore, the cyanobacterium was phylogenetically distinct from Prochloron , strongly suggesting that it should be a sister taxon to Prochloron . Therefore, we proposed this symbiotic cyanobacterium as a novel species under the new genus Candidatus Paraprochloron terpiosi. Comparative genomic analyses revealed that ‘Paraprochloron’ and Prochloron exhibit distinct genomic features and DNA replication machinery. We also characterized the metabolic potentials of ‘Paraprochloron terpiosi’ in carbon and nitrogen cycling and propose a model for interactions between it and T. hoshinota . This study builds a foundation for the study of the T . hoshinota microbiome and paves the way for better understanding of ecosystems involving this coral‐killing sponge.

On the Dependency of Bottom Drag and the Eddy Viscosity upon Flow Structure in the Coastal Boundary Layer - Yao-Zhao Zhong; Hwa Chien; Meng-Yu Lin; Anna Wargula; Jia-Lin Chen (Journal of Marine Science and Engineering, 2022)
The physical processes governing coastal exchange between the surf zone, the inner shelf, and the open ocean is critical for estimating mass exchange and its impact on ecological processes. The present study combined field measurements and theoretical approaches to explore the hydrodynamics in the coastal boundary layer (CBL) in which both bottom drag and shore friction affect the transport and mixing processes. Observed drifter-cluster trajectories in a nearly alongshore-uniform coastal area showed that the occurrence of current reversal varies with cross-shore distance, which confirmed the tidal phase difference between different cross-shore distances predicted by the proposed CBL model. According to the CBL model, tidal phase difference is affected by the bottom drag coefficient and horizontal eddy viscosity coefficient. With the results of three experiments under different wave conditions, this study also discusses the effects of waves on the CBL. Data analysis based on observations indicates that the bottom drag term is closely related to the bottom shear stress induced by the interactions of waves and currents. The bottom drag coefficient under the more energetic wave condition was much greater than that under milder wave conditions during the experiment. The study also suggests that in addition to pressure gradient and bottom drag, flow structure is subject to lateral stress, which reflects the impact of shoreline roughness in the nearshore region and that the estimated eddy viscosity coefficient decreases linearly with distance from the shoreline.

A First Insight Into the Heat-Induced Changes in Proteomic Profiles of the Coral Symbiotic Bacterium Endozoicomonas montiporae - Ya-Fan Chan; Chia-Yu Chen; Chih-Ying Lu; Yung-Chi Tu; Kshitij Tandon; Shinya Shikina; Sen-Lin Tang (Frontiers in Marine Science, 2022)
Endozoicomonas , a core bacterial group in corals, may also be a coral symbiont. Endozoicomonas communities often decrease rapidly in corals under heat stress. However, how the bacteria respond to changes in temperature and coral host during heat stress is unknown. Here, we employed the cultivable, dominant species E. montiporae as a working organism to explore how Endozoicomonas responds to heat stress. We designed two experiments to clarify the extent to which E. montiporae is influenced by temperature and coral host. We detected differentially expressed protein (DEP) profiles in this bacterium at 31 and 33°C compared to 25°C by tandem mass tags-based quantitative proteome analysis. Fifty DEPs, including many heat shock proteins, were detected when the temperature changed. The expression of antioxidant defense proteins and key pyruvate synthase proteins decreased, suggesting that E. montiporae were in a physiology of stress at 33°C. Furthermore, some proteins were differentially expressed because of the heat-stress-treated coral lysate specifically, suggesting that not only heat but also heat-induced host factors can affect the protein expression of the bacterium. This study provides an in-depth analysis of how the molecular mechanisms of Endozoicomonas are affected by heat stress and coral host.

Fine mapping of the tiller inhibition gene TIN4 contributing to ideal plant architecture in common wheat - Zhiqiang Wang; Fangkun Wu; Xudong Chen; Wanlin Zhou; Haoran Shi; Yu Lin; Shuai Hou; Shifan Yu; Hong Zhou; Caixia Li; Yaxi Liu (Theoretical and Applied Genetics, 2022)

Oxygen Metabolism-induced Stress Response Underlies Heart–brain Interaction Governing Human Consciousness-breaking and Attention - Xiao-Juan Xue; Rui Su; Ze-Feng Li; Xiao-Ou Bu; Peng Dang; Si-Fang Yu; Zhi-Xin Wang; Dong-Mei Chen; Tong-Ao Zeng; Ming Liu; Hai-Lin Ma; De-Long Zhang (Neuroscience Bulletin, 2022)

Mucormycosis in Mainland China: A Systematic Review of Case Reports - Lin-Wei Wei; Pei-Qiu Zhu; Xiao-Qing Chen; Jin Yu (Mycopathologia, 2022)

Characteristics of typical air pollutants in a valley-basin city in South China - Chu Wu; Jibing Li; Xiaoping Lin; Yangzhi Mo; Hui Zhang; Zhihui Liao; Yu Wang; Duohong Chen (Meteorology and Atmospheric Physics, 2022)

Full-Length Transcriptome of Red Swamp Crayfish Hepatopancreas Reveals Candidate Genes in Hif-1 and Antioxidant Pathways in Response to Hypoxia-Reoxygenation - Yu Xu; Hai Lin; Weihui Yan; Jiajia Li; Mengling Sun; Jiaping Chen; Zhiqiang Xu (Marine Biotechnology, 2022)

Consistent responses of microbial C and N metabolic processes to elevated CO2 across global terrestrial ecosystems - Jiahui Lin; Yanlan Huang; Haochun Zhao; Mengjie Yu; Weiqin Su; Huaihai Chen; Peng Leng; Jihui Li; Yu Luo; Yong Li; Zhongmin Dai; Jianming Xu (Journal of Soils and Sediments, 2022)

New paraconic acids from the endophytic fungus Xylariaceae sp. J4 - Chao Yuan; Xue-Chun Shu; Lin Fan; Ying-Bo Zhang; Ling-Liang Guan; Fu-Lai Yu; Xiao-Lu Chen; Gang Li (Natural Product Research, 2022)

Distinct durability of IgM/IgG antibody responses in COVID-19 patients with differing severity - Junyan Han; Ning Zhang; Danying Chen; Yuhuan Gong; Guoli Li; Yaxian Kong; Lin Pu; Chen Chen; Jingyuan Liu; Qi Wang; Ju Zhang; Xuesen Zhao; et al. (Science China Life Sciences, 2022)

MAPK5 and MAPK10 overexpression influences strawberry fruit ripening, antioxidant capacity and resistance to Botrytis cinerea - Yunting Zhang; Yu Long; Yiting Liu; Min Yang; Liangxin Wang; Xiaoyang Liu; Yong Zhang; Qing Chen; Mengyao Li; Yuanxiu Lin; Haoru Tang; Ya Luo (Planta, 2022)

Celastrol attenuates chronic constrictive injury-induced neuropathic pain and inhibits the TLR4/NF-κB signaling pathway in the spinal cord - Gui-juan Jin; Xuehuizi Peng; Zhi-Guo Chen; Yu-lin Wang; Wen-jun Liao (Journal of Natural Medicines, 2022)

A novel alphahypovirus that infects the fungal plant pathogen Sclerotinia sclerotiorum - Weibo Liang; Zhongbo Lu; Jie Duan; Daohong Jiang; Jiatao Xie; Jiasen Cheng; Yanping Fu; Tao Chen; Bo Li; Xiao Yu; Weidong Chen; Yang Lin (Archives of Virology, 2022)

Nakamurella leprariae sp. nov., isolated from a lichen sample - De-Feng An; Shao-Juan Yang; Long-Qian Jiang; Xin-Yu Wang; Xiao-Yu Huang; Lei Lang; Xue-Mei Chen; Ming-Qun Fan; Gui-Ding Li; Ming-Guo Jiang; Li-Song Wang; Cheng-Lin Jiang; et al. (Archives of Microbiology, 2022)

The brown root rot fungus Phellinus noxius affects microbial communities in different root‐associated niches of Ficus trees - Tse‐Yen Liu; Chao‐Han Chen; Yu‐Liang Yang; Isheng J. Tsai; Ying‐Ning Ho; Chia‐Lin Chung (Environmental Microbiology, 2022)
Summary Brown root rot (BRR) caused by Phellinus noxius is a destructive tree disease in tropical and subtropical areas. To understand how BRR affects the composition of the plant rhizoplane‐enriched microbiota, the microbiomes within five root‐associated compartments (i.e., bulk soil, old/young root rhizosphere soil, old/young root tissue) of Ficus trees naturally infected by P. noxius were investigated. The level of P. noxius infection was determined by quantitative PCR. Illumina sequencing of the internal transcribed spacer and 16S rRNA revealed that P. noxius infection caused a significant reduction in fungal diversity in the bulk soil, the old root rhizosphere soil, and the old root tissue. Interestingly, Cosmospora was the only fungal genus positively correlated with P. noxius . The abundance and composition of dominant bacterial taxa such as Actinomadura , Bacillus , Rhodoplanes , and Streptomyces differed between BRR‐diseased and healthy samples. Furthermore, 838 isolates belonging to 26 fungal and 35 bacterial genera were isolated and tested for interactions with P. noxius . Antagonistic activities were observed for isolates of Bacillus , Pseudomonas , Aspergillus , Penicillium , and Trichoderma . Cellophane overlay and cellulose/lignin utilization assays suggested that Cosmospora could tolerate the secretions of P. noxius and that the degradation of lignin by P. noxius may create suitable conditions for Cosmorpora growth.

Interannual Abundance Fluctuations of Two Oceanic Squids in the Pacific Ocean Can Be Evaluated Through Their Habitat Temperature Variabilities - Peng Chen; Xinjun Chen; Wei Yu; Dongming Lin (Frontiers in Marine Science, 2021)
Neon flying squid ( Ommastrephes bartramii ) and jumbo flying squid ( Dosidicus gigas ) are two commercially essential oceanic squids in the Pacific Ocean. An in-depth understanding of the mechanisms of their annual and interannual abundance fluctuations under environmental and climate variabilities can ensure ration and suitable management. Thus, the annual and interannual abundance fluctuations of the stock of the western winter-spring cohort of O. bartramii and D. gigas off Peru Exclusive Economic Zone (PEEZ) waters and their association with habitat temperature variabilities are explored in this study based on the historical Chinese Squid-jigging fishery data from 2003 to 2020. The habitat temperature variabilities were defined as the effective principal components of the SST at the squids’ two important habitats (spawning and feeding ground) through life histories according to the principal component analysis. The Hodrick—Prescott filter analysis was conducted to quantify the annual and interannual fluctuations of abundance and habitat temperature variabilities. Furthermore, the generalized additive model (GAM) was employed to investigate their associations. The results demonstrated different but not synchronous trends of abundance for O. bartramii and D. gigas . Regarding O. bartramii , the interannual abundance first decreased (2003∼2013) and then increased (after 2014). For D. gigas , the interannual abundance kept decreasing within 2003∼2020. Their annual trends have presented large fluctuations over years. The results of GAM indicated that using habitat temperature variabilities only can trace the abundance trend of O. bartramii and D. gigas at an interannual but not annual scale. Further studies verified that Pacific Decadal Oscillation (PDO) is a crucial factor affecting the interannual abundance trend of these two squids through the habitat temperature variabilities. However, this study indicates that the habitat temperature variabilities not only are related to other large-scale factors, which are not investigated currently at an interannual scale, but also, are combined with other small- or middle-scale factors at an annual scale on their impacts to the abundance fluctuations of these two squids. Therefore, in order to better explain the mechanisms of annual and interannual abundance fluctuations of O. bartramii and D. gigas under environmental and climate variabilities, the importance of combining other potential factors into consideration is highlighted.

The Effects of Limosilactobacillus reuteri LR-99 Supplementation on Body Mass Index, Social Communication, Fine Motor Function, and Gut Microbiome Composition in Individuals with Prader–Willi Syndrome: a Randomized Double-Blinded Placebo-Controlled Trial - Xue-Jun Kong; Kevin Liu; Patrick Zhuang; Ruiyi Tian; Siyu Liu; Cullen Clairmont; Xiaojing Lin; Hannah Sherman; Junli Zhu; Yelan Wang; Michelle Fong; Alice Li; et al. (Probiotics and Antimicrobial Proteins, 2021)
Prader–Willi syndrome (PWS) is a rare genetic disorder associated with developmental delay, obesity, and neuropsychiatric comorbidities. Limosilactobacillus reuteri ( Lactobacillus reuteri , Lact. reuteri ) has demonstrated anti-obesity and anti-inflammatory effects in previous studies. In the present study, we aim to evaluate the effects of Lact. reuteri supplementation on body mass index (BMI), social behaviors, and gut microbiota in individuals with PWS. We conducted a 12-week, randomized, double-blind, placebo-controlled trial in 71 individuals with PWS aged 6 to 264 months (64.4 ± 51.0 months). Participants were randomly assigned to either receive daily Lact. reuteri LR-99 probiotic (6 × 10 10 colony forming units) or a placebo sachet. Groupwise differences were assessed for BMI, ASQ-3, and GARS-3 at baseline, 6 weeks, and 12 weeks into treatment. Gut microbiome data was analyzed with the QIIME2 software package, and predictive functional profiling was conducted with PICRUSt-2. We found a significant reduction in BMI for the probiotic group at both 6 weeks and 12 weeks relative to the baseline ( P < 0.05). Furthermore, we observed a significant improvement in social communication and interaction, fine motor function, and total ASQ-3 score in the probiotics group compared to the placebo group ( P < 0.05). Altered gut microbiota was observed in the probiotic group to favor weight loss and improve gut health. The findings suggest a novel therapeutic potential for Lact. reuteri LR-99 probiotic to modulate BMI, social behaviors, and gut microbiota in Prader–Willi syndrome patients, although further investigation is warranted. Trial registration Chinese Clinical Trial Registry: ChiCTR1900022646

Serum Neurofilament Light: a Potential Diagnostic and Prognostic Biomarker in Obstetric Posterior Reversible Encephalopathy Syndrome - Xiaobo Fang; Yanling Liang; Weixi Zhang; Qiong Wang; Jingsi Chen; Jia Chen; Yongqiang Lin; Yanli Chen; Li Yu; Haibin Wang; Dunjin Chen (Molecular Neurobiology, 2021)

Measuring Heterogeneity in 18F-Fluorodeoxyglucose Positron Emission Tomography Images for Classifying Metastatic and Benign Bone Lesions in Patients with Cervical Cancer - Feng-Yuan Liu; Gigin Lin; Jing-Ren Tseng; Angel Chao; Huei-Jean Huang; Hung-Hsueh Chou; Yu-Chen Chang; Tzu-Chen Yen; Chyong-Huey Lai (Journal of Medical and Biological Engineering, 2021)
Purpose Heterogeneity assessment can be applied for medical imaging analysis. Here, we evaluated first-order and texture analysis (TA) metrics in 18 F-fluorodeoxyglucose ( 18 F-FDG) positron emission tomography (PET) imaging for classification of metastatic and benign bone lesions in patients with cervical cancer. Methods The data of 18 F-FDG PET studies performed on a specific PET/CT system from 2016 to 2018 in patients with cervical cancer were retrieved. The data of bone lesions extracted from studies over 2016–2017 and 2018 were used as training and validation datasets, respectively. Metastatic bone lesions were identified in each dataset, with an equal number of benign bone lesions selected. Cuboid volume of interest (VOI) consisting of 3 × 3 × 5 reconstructed voxels was applied for first-order metrics, and cubic VOI consisting of smaller voxels with trilinear interpolation of standardized uptake value (SUV) was adopted for TA metrics. First-order metrics included the maximum SUV (SUVmax) of lesions and the mean voxel SUV and its standard deviation (SUVsd), skewness, and kurtosis in VOI. In total, 4464 TA metrics based on 62 texture features were evaluated. Logistic regression was used for classification with area under the receiver operating characteristic curve (AUC) as the performance measure. Results From the training and validation datasets, 98 and 42 metastatic bone lesions were identified, respectively. SUVsd demonstrated higher performance than did SUVmax in both the training (AUC .798 vs .732, P = .001) and validation (AUC .786 vs .684, P < .001) datasets. Top-performing TA metrics demonstrated significantly higher performance in the training dataset, but not in the validation dataset. Conclusion A simple first-order measure of heterogeneity, SUVsd, was found to be superior to SUVmax for the classification of metastatic and benign bone lesions. Multiple hypothesis testing can result in false-positive findings in TA with multiple features and parameters; careful validation is required.

A comparison of atmospheric geochemistry through lichens from volcanic and non-volcanic areas, north Taiwan - Le-Qi Lin; Hsueh-Yu Lu; Ju-Lien Pi; Tai-Sheng Liou; Wen-Fu Chen; Pei-Shan Hsieh (Journal of Earth System Science, 2021)

Expression profiling of WRKY transcription factors in Spinach - Yaying Ma; Shuai Chen; Hongying Yu; Ruoyu Li; Zhicong Lin; Mingxing Cai; Min Tang; Xingtan Zhang; Ray Ming (Euphytica, 2021)

Development and characterization of SNP markers in many-lined sun skink (Eutropis multifasciata) from transcriptomic sequences - Youfu Lin; Zijin Qian; Shiyu Shen; Yicheng Huang; Yu Du; Hong Li; Lian Chen (Conservation Genetics Resources, 2021)

Termite mound formation reduces the abundance and diversity of soil resistomes - Zhen‐Zhen Yan; Qing‐Lin Chen; Chao‐Yu Li; Bao‐Anh Thi Nguyen; Yong‐Guan Zhu; Ji‐Zheng He; Hang‐Wei Hu (Environmental Microbiology, 2021)
Summary Termites are pivotal ecosystem engineers in tropical and subtropical habitats, where they construct massive nests (‘mounds’) that substantially modify soil properties and promote nutrient cycling. Yet, little is known about the roles of termite nesting activity in regulating the spread of antimicrobial resistance (AMR), one of the major Global Health challenges. Here, we conducted a large‐scale (> 1500 km) investigation in northern Australia and found distinct resistome profiles in termite mounds and bulk soils. By profiling a wide spectrum of ARGs, we found that the abundance and diversity of antibiotic resistance genes (ARGs) were significantly lower in termite mounds than in bulk soils ( P P

Precipitation increases the abundance of fungal plant pathogens in Eucalyptus phyllosphere - Qing‐Lin Chen; Hang‐Wei Hu; Zhen‐Zhen Yan; Chao‐Yu Li; Bao‐Anh Thi Nguyen; Yong‐Guan Zhu; Ji‐Zheng He (Environmental Microbiology, 2021)
Summary Understanding the current and future distributions of plant pathogens is critical to predict the plant performance and related economic benefits in the changing environment. Yet, little is known about the roles of environmental drivers in shaping the profiles of fungal plant pathogens in phyllosphere, an important habitat of microbiomes on Earth. Here, using a large‐scale investigation of Eucalyptus phyllospheric microbiomes in Australia and the multiple linear regression model, we show that precipitation is the most important predictor of fungal taxonomic diversity and abundance. The abundance of fungal plant pathogens in phyllosphere exhibited a positive linear relationship with precipitation. With this empirical dataset, we constructed current and future atlases of phyllosphere plant pathogens to estimate their spatial distributions under different climate change scenarios. Our atlases indicate that the abundance of fungal plant pathogens would increase especially in the coastal regions with up to 100‐fold increase compared with the current abundance. These findings advance our understanding of the distributions of fungal plant pathogens in phyllospheric microbiomes under the climate change, which can improve our ability to predict and mitigate their impacts on plant productivity and economic losses.

A New Instrumental Array in Sichuan, China, to Monitor Vibrations and Perturbations of the Lithosphere, Atmosphere, and Ionosphere - Chieh-Hung Chen; Yang-Yi Sun; Kai Lin; Chen Zhou; Rui Xu; Haiyin Qing; Yongxin Gao; Tao Chen; Fei Wang; Huaizhong Yu; Peng Han; Chi-Chia Tang; et al. (Surveys in Geophysics, 2021)

Harmful algal bloom-forming dinoflagellate Prorocentrum donghaiense inhibits the growth and photosynthesis of seaweed Sargassum fusiformis embryos - Caixia Wang; Min Wang; Binbin Chen; Wenli Qin; Lidong Lin; Chuanjun Dai; Hengguo Yu; Renhui Li; Min Zhao; Zengling Ma (Journal of Oceanology and Limnology, 2021)

Streptococcus vaginalis sp. nov., a novel bacterial species isolated from vaginal swabs of a pregnant woman with diabetes - An-Shine Chao; Chiao-Yun Lin; Angel Chao; Yun-Shien Lee; Yu-Chung Chang; Hui-Chung Wu; Yu-Jie Dai; Wei-Qi Liao; Fan-Ching Kao; Yi-Sheng Chen (Archives of Microbiology, 2021)

Pseudomonas nanhaiensis sp. nov., a lipase-producing bacterium isolated from deep-sea sediment of the South China Sea - Yu Pang; Yage Zhang; Mengru Chen; Wei Lu; Ming Chen; Yongliang Yan; Min Lin; Wei Zhang; Zhengfu Zhou (Antonie van Leeuwenhoek, 2021)

Wave-ice dynamical interaction: a numerical model and its application - Yang Zhang; Changsheng Chen; Guoping Gao; Jianhua Qi; Huichan Lin; Wei Yu; Liang Chang (Acta Oceanologica Sinica, 2021)

Complement C3 Aggravates Post-epileptic Neuronal Injury Via Activation of TRPV1 - Guang-Tong Jiang; Lin Shao; Shuo Kong; Meng-Liu Zeng; Jing-Jing Cheng; Tao-Xiang Chen; Song Han; Jun Yin; Wan-Hong Liu; Xiao-Hua He; Yu-Min Liu; Lanzi Gongga; et al. (Neuroscience Bulletin, 2021)

Chronic Exposure to Hypoxia Inhibits Myelinogenesis and Causes Motor Coordination Deficits in Adult Mice - Lin Chen; Shu-Yu Ren; Rui-Xue Li; Kun Liu; Jing-Fei Chen; Yu-Jian Yang; Yong-Bin Deng; Han-Zhi Wang; Lan Xiao; Feng Mei; Fei Wang (Neuroscience Bulletin, 2021)
Exposure to chronic hypoxia is considered to be a risk factor for deficits in brain function in adults, but the underlying mechanisms remain largely unknown. Since active myelinogenesis persists in the adult central nervous system, here we aimed to investigate the impact of chronic hypoxia on myelination and the related functional consequences in adult mice. Using a transgenic approach to label newly-generated myelin sheaths ( NG2-CreER TM ; Tau-mGFP ), we found that myelinogenesis was highly active in most brain regions, such as the motor cortex and corpus callosum. After exposure to hypoxia (10% oxygen) 12 h per day for 4 weeks, myelinogenesis was largely inhibited in the 4-month old brain and the mice displayed motor coordination deficits revealed by the beam-walking test. To determine the relationship between the inhibited myelination and functional impairment, we induced oligodendroglia-specific deletion of the transcription factor Olig2 by tamoxifen ( NG2-CreER TM ; Tau-mGFP; Olig2 fl/fl ) in adult mice to mimic the decreased myelinogenesis caused by hypoxia. The deletion of Olig2 inhibited myelinogenesis and consequently impaired motor coordination, suggesting that myelinogenesis is required for motor function in adult mice. To understand whether enhancing myelination could protect brain functions against hypoxia, we treated hypoxic mice with the myelination-enhancing drug-clemastine, which resulted in enhanced myelogenesis and improved motor coordination. Taken together, our data indicate that chronic hypoxia inhibits myelinogenesis and causes functional deficits in the brain and that enhancing myelinogenesis protects brain functions against hypoxia-related deficits.

Three new species of Cylindrobasidium (Physalacriaceae, Agaricales) from East Asia - Yi-Chung Lin; Chia-Ling Wei; Chi-Yu Chen; Che-Chih Chen; Sheng-Hua Wu (Mycological Progress, 2021)

Meta-analyses of maternal exposure to atmospheric particulate matter and risk of congenital anomalies in offspring - Guangxia Yu; Yao Chen; Jianping Tang; Zhifeng Lin; Fuli Zheng; Chunyan Zheng; Jinfu Zhou; Qianqian Su; Siying Wu; Huangyuan Li (Environmental Science and Pollution Research, 2021)

Sandaracinobacteroides hominis gen. nov., sp. nov., isolated from human skin - Ping-Hua Qu; Hai-Min Luo; Jun-Hui Feng; Song Li; Cha Chen; Lei Dong; Yu-Zhen Ming; Wen-Jun Li; Ying Lin (Archives of Microbiology, 2021)

Correction to: A failure case study of tunnel water inrush source identified by statistical analysis model - Li-Gang Yuan; Xiao-Li Li; Xin Li; Yi-Lin Yu; Li-Guang Chen; Xian-Pan Xu; Xiang Wang (Arabian Journal of Geosciences, 2021)

Three-dimensional properties of mesoscale cyclonic warm-core and anticyclonic cold-core eddies in the South China Sea - Wenjin Sun; Yu Liu; Gengxin Chen; Wei Tan; Xiayan Lin; Yuping Guan; Changming Dong (Acta Oceanologica Sinica, 2021)

Maternal Subclinical Hypothyroidism in Rats Impairs Spatial Learning and Memory in Offspring by Disrupting Balance of the TrkA/p75NTR Signal Pathway - Fan Zhang; Xinyue Lin; Aihua Liu; Jian Chen; Zhongyan Shan; Weiping Teng; Xiaohui Yu (Molecular Neurobiology, 2021)
Maternal subclinical hypothyroidism (SCH) during pregnancy can adversely affect the neurodevelopment of the offspring. The balance of nerve growth factor (NGF)-related tropomyosin receptor kinase A/p75 neurotrophin receptor (TrkA/p75 NTR ) signaling in the hippocampus is important in brain development, and whether it affects cognitive function in maternal SCH’s offspring is not clear. In this study, we found that compared with the control (CON) group, expression of proliferation-related proteins [NGF, p-TrkA, phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2) and phospho-cAMP response element-binding protein (p-CREB)] decreased in the hippocampus of the offspring in the SCH group, overt hypothyroidism (OHT) group, and the group with levothyroxine (L-T 4 ) treatment for SCH from gestational day 17 (E17). In contrast, expression of apoptosis-related proteins [pro-NGF, p75 NTR , phospho-C-Jun N-terminal kinase (p-JNK), p53, Bax and cleaved caspase-3] was increased. The two groups with treatment with L-T 4 for SCH from E10 and E13, respectively, showed no significant difference compared with the CON group. L-T 4 treatment enhanced relative expression of NGF by increasing NGF/proNGF ratio in offspring from maternal SCH rats. In conclusion, L-T 4 treatment for SCH from early pregnancy dramatically ameliorated cognitive impairment via TrkA/p75 NTR signaling, which involved activation of the neuronal proliferation and inhibition of neuronal apoptosis in SCH rats’ offspring.

miR-145-5p targets paxillin to attenuate angiotensin II-induced pathological cardiac hypertrophy via downregulation of Rac 1, pJNK, p-c-Jun, NFATc3, ANP and by Sirt-1 upregulation - Kuan-Ho Lin; V. Bharath Kumar; Tamilselvi Shanmugam; Marthandam Asokan Shibu; Ray-Jade Chen; Chia-Hua Kuo; Tsung-Jung Ho; V. Vijaya Padma; Yu-Lan Yeh; Chih-Yang Huang (Molecular and Cellular Biochemistry, 2021)

Integration of small RNAs, degradome, and transcriptome sequencing provides insights into the differences between Shizhu ginseng and Yuan ginseng - Mengyuan Peng; Yanlin Chen; Wenjuan Wang; Zhihua He; Wangqiang Dai; Zhiyun Lin; Zemin Yang; Mengjuan Gong; Yongqin Yin; Bin Han; Yu Zeng; Yingfang Wang (Journal of Plant Biochemistry and Biotechnology, 2021)
Panax ginseng is one of the most popular herbs which have been used as an important traditional Chinese medicine since ancient times. Yuan ginseng and Shizhu ginseng,which belong to P. ginseng , are widely used as substitutes for wild ginseng in clinical practice. Clinical practice has proved that the clinical efficacy of Shizhu ginseng is better than Yuan ginseng. However, current research cannot completely explain this phenomenon. Considering that small RNA may be one of the pharmacodynamic substances of P. ginseng , it is challenging to investigate differential miRNAs between Shizhu ginseng and Yuan ginseng. In this study, the transcriptome, small RNAome and degradome of P. ginseng were studied by high-throughput sequencing. A total of 63,875 unigenes and 43,950,137 small RNA clean reads were obtained from the roots of P. ginseng . Among 3206 differentially expressed genes, 1190 genes were up-regulated in Yuan ginseng when compared with Shizhu ginseng. 24 known differential miRNAs and 7 novel differential miRNAs were obtained. The 304 targets of 24 differentially expressed miRNA (17 known and 7 novel) families are mainly related to energy metabolism, biotic stress and disease immunity in ginseng itself. Through the association analysis of mRNA and miRNA, our work gives a better understanding of the difference between Yuan ginseng and Shizhu ginseng. Considering the cross-kingdom regulation of plant miRNAs, our results may provide a foundation for understanding the miRNA-dependent clinical efficacy in P. ginseng .

High-throughput sequencing clarifies the spatial structures of microbial communities in cadmium-polluted rice soils - Li Song; Zhenzhi Pan; Yi Dai; Lin Chen; Li Zhang; Qilin Liao; Xiezhi Yu; Hongyan Guo; Guisheng Zhou (Environmental Science and Pollution Research, 2021)

Acute associations between air pollution on premature rupture of membranes in Hefei, China - Chao Zhang; Sha Li; Gan-lan Guo; Jing-wen Hao; Peng Cheng; Li-lin Xiong; Shu-ting Chen; Ji-yu Cao; Yu-wen Guo; Jia-hu Hao (Environmental Geochemistry and Health, 2021)

Viable and Heat-Killed Probiotic Strains Improve Oral Immunity by Elevating the IgA Concentration in the Oral Mucosa - Wen-Yang Lin; Yi-Wei Kuo; Ching-Wei Chen; Yu-Fen Huang; Chen-Hung Hsu; Jia-Hung Lin; Cheng-Ruei Liu; Jui-Fen Chen; Ko-Chiang Hsia; Hsieh-Hsun Ho (Current Microbiology, 2021)
Oral-nasal mucosal immunity plays a crucial role in protecting the body against bacterial and viral invasion. Safe probiotic products have been used to enhance human immunity and oral health. In this study, we verified the beneficial effects of mixed viable probiotic tablets, consisting of Lactobacillus salivarius subsp. salicinius AP-32, Bifidobacterium animalis subsp. lactis CP-9, and Lactobacillus paracasei ET-66, and heat-killed probiotic tablets, consisting of L. salivarius subsp. salicinius AP-32 and L. paracasei ET-66, on oral immunity among 45 healthy participants. Participants were randomly divided into viable probiotic, heat-killed probiotic, and placebo groups. The administration of treatment lasted for 4 weeks. Saliva samples were collected at Weeks 0, 2, 4, and 6, and Lactobacillus , Bifidobacterium and Streptococcus mutans populations and IgA concentration were measured. IgA concentrations, levels of TGF-beta and IL-10 in PBMCs cells were quantified by ELISA method. Results showed that salivary IgA levels were significantly increased on administration of both the viable (119.30 ± 12.63%, *** P < 0.001) and heat-killed (116.78 ± 12.28%, *** P < 0.001) probiotics for 4 weeks. Among three probiotic strains, AP-32 would effectively increase the levels of TGF-beta and IL-10 in PBMCs. The oral pathogen Streptococcus mutans was significantly reduced on viable probiotic tablet administration (49.60 ± 31.01%, *** P < 0.001). The in vitro antibacterial test confirmed that viable probiotics effectively limited the survival rate of oral pathogens. Thus, this clinical pilot study demonstrated that oral probiotic tablets both in viable form or heat-killed form could exert beneficial effects on oral immunity via IL-10, TGB-beta mediated IgA secretion. The effective dosage of viable probiotic content in the oral tablet was 10 9 CFUs/g and the heat-killed oral tablet was 1 × 10 10 cells/g.

Protective effect of Bombyx mori gloverin on intestinal epithelial cells exposure to enterotoxigenic E. coli - Qian Lin; Qingqing Fu; Guoqi Su; Daiwen Chen; Bing Yu; Yuheng Luo; Ping Zheng; Xiangbing Mao; Zhiqing Huang; Jie Yu; Junqiu Luo; Hui Yan; et al. (Brazilian Journal of Microbiology, 2021)

Complete genome analysis of the newly isolated Shigella sonnei phage vB_SsoM_Z31 - Bingdong Wei; Cong Cong; Lichun Zhang; Lin Zheng; Long Chen; Wei Yu; Yongping Xu (Archives of Virology, 2021)

A failure case study of tunnel water inrush source identified by statistical analysis model - Li-Gang Yuan; Xiao-Li Li; Xin Li; Yi-Lin Yu; Li-Gang Chen; Xian-Pan Xu; Xiang Wang (Arabian Journal of Geosciences, 2021)

Anti-Inflammatory and anti-proliferative oleanane-type triterpene glycosides from the vine of Momordica cochinchinensis - Hung-Tse Huang; Yu-Chi Lin; Li-Jie Zhang; Chia-Ching Liaw; Hsin-Yen Chen; Ming-Tung Hsueh; Yao-Haur Kuo (Natural Product Research, 2021)

Wogonin Accelerates Hematoma Clearance and Improves Neurological Outcome via the PPAR-γ Pathway After Intracerebral Hemorrhage - Jianfeng Zhuang; Yucong Peng; Chi Gu; Huihui Chen; Zheng Lin; Hang Zhou; Xiao Wu; Jianru Li; Xiaobo Yu; Yang Cao; Hanhai Zeng; Xiongjie Fu; et al. (Translational Stroke Research, 2021)

The major photoprotective role of anthocyanins in leaves of Arabidopsis thaliana under long-term high light treatment: antioxidant or light attenuator? - Xiao-Ting Zheng; Zheng-Chao Yu; Jun-Wei Tang; Min-Ling Cai; Yi-Lin Chen; Cheng-Wei Yang; Wah Soon Chow; Chang-Lian Peng (Photosynthesis Research, 2021)

Dicer Deletion in Astrocytes Inhibits Oligodendroglial Differentiation and Myelination - Kun Liu; Bin Yu; Jing-Fei Chen; Rui-Xue Li; Lin Chen; Shu-Yu Ren; Fei Wang; Feng Mei; Lan Xiao (Neuroscience Bulletin, 2021)
Increasing evidence has shown that astrocytes are implicated in regulating oligodendrocyte myelination, but the underlying mechanisms remain largely unknown. To understand whether microRNAs in astrocytes function in regulating oligodendroglial differentiation and myelination in the developing and adult CNS, we generated inducible astrocyte-specific Dicer conditional knockout mice (hGFAP-CreERT; Dicer fl/fl). By using a reporter mouse line (mT/mG), we confirmed that hGFAP-CreERT drives an efficient and astrocyte-specific recombination in the developing CNS, upon tamoxifen treatment from postnatal day 3 (P3) to P7. The Dicer deletion in astrocytes resulted in inhibited oligodendroglial differentiation and myelination in the developing CNS of Dicer cKO mice at P10 and P14, and did not alter the densities of neurons or axons, indicating that Dicer in astrocytes is required for oligodendrocyte myelination. Consequently, the Dicer deletion in astrocytes at P3 resulted in impaired spatial memory and motor coordination at the age of 9 weeks. To understand whether Dicer in astrocytes is also required for remyelination, we induced Dicer deletion in 3-month-old mice and then injected lysolecithin into the corpus callosum to induce demyelination. The Dicer deletion in astrocytes blocked remyelination in the corpus callosum 14 days after induced demyelination. Together, our results indicate that Dicer in astrocytes is required for oligodendroglia myelination in both the developing and adult CNS.

GABA Signaling Pathway-associated Gene PLCL1 Rare Variants May be Associated with Autism Spectrum Disorders - Fengyun Zheng; Guoyuan Liu; Ting Dang; Qiaowen Chen; Yu An; Meng Wu; Xiangxuan Kong; Zilong Qiu; Bai-Lin Wu (Neuroscience Bulletin, 2021)

Nucleophosmin/B23 promotes endometrial cancer cell escape from macrophage phagocytosis by increasing CD24 expression - Chiao-Yun Lin; Chia-Lung Tsai; Angel Chao; Li-Yu Lee; Wei-Chun Chen; Yun-Hsin Tang; An-Shine Chao; Chyong-Huey Lai (Journal of Molecular Medicine, 2021)

Performance Comparison of the Deep Learning and the Human Endoscopist for Bleeding Peptic Ulcer Disease - Hsu-Heng Yen; Ping-Yu Wu; Pei-Yuan Su; Chia-Wei Yang; Yang-Yuan Chen; Mei-Fen Chen; Wen-Chen Lin; Cheng-Lun Tsai; Kang-Ping Lin (Journal of Medical and Biological Engineering, 2021)
Purpose Management of peptic ulcer bleeding is clinically challenging. Accurate characterization of the bleeding during endoscopy is key for endoscopic therapy. This study aimed to assess whether a deep learning model can aid in the classification of bleeding peptic ulcer disease. Methods Endoscopic still images of patients ( n = 1694) with peptic ulcer bleeding for the last 5 years were retrieved and reviewed. Overall, 2289 images were collected for deep learning model training, and 449 images were validated for the performance test. Two expert endoscopists classified the images into different classes based on their appearance. Four deep learning models, including Mobile Net V2, VGG16, Inception V4, and ResNet50, were proposed and pre-trained by ImageNet with the established convolutional neural network algorithm. A comparison of the endoscopists and trained deep learning model was performed to evaluate the model’s performance on a dataset of 449 testing images. Results The results first presented the performance comparisons of four deep learning models. The Mobile Net V2 presented the optimal performance of the proposal models. The Mobile Net V2 was chosen for further comparing the performance with the diagnostic results obtained by one senior and one novice endoscopists. The sensitivity and specificity were acceptable for the prediction of “normal” lesions in both 3-class and 4-class classifications. For the 3-class category, the sensitivity and specificity were 94.83% and 92.36%, respectively. For the 4-class category, the sensitivity and specificity were 95.40% and 92.70%, respectively. The interobserver agreement of the testing dataset of the model was moderate to substantial with the senior endoscopist. The accuracy of the determination of endoscopic therapy required and high-risk endoscopic therapy of the deep learning model was higher than that of the novice endoscopist. Conclusions In this study, the deep learning model performed better than inexperienced endoscopists. Further improvement of the model may aid in clinical decision-making during clinical practice, especially for trainee endoscopist.

Risk of two common glandular cell-type cancers (breast and colorectal cancers) in Chinese occupational chefs: a nationwide ecological study in Taiwan - Pei-Chen Lin; Chiung‑Yu Peng; Chih‑Hong Pan; Jui-Ying Lee; Tusty-Jiuan Hsieh; Yun-Shiuan Chuang; Robert J. Turesky; Chia-Fang Wu; Ming-Tsang Wu (International Archives of Occupational and Environmental Health, 2021)

Hydrogen alleviates cell damage and acute lung injury in sepsis via PINK1/Parkin-mediated mitophagy - Hongguang Chen; Huaying Lin; Beibei Dong; Yaoqi Wang; Yonghao Yu; Keliang Xie (Inflammation Research, 2021)

Characteristics of bacterial community and ARGs profile in engineered goldfish tanks with stresses of sulfanilamide and copper - Benjamin Makimilua Tiimub; Zhen-chao Zhou; Lin Zhu; Yang Liu; Xin-Yi Shuai; Lan Xu; Christophe’ Niyungeko; Ling-Xuan Meng; Yu-Jie Sun; Hong Chen (Environmental Science and Pollution Research, 2021)

Porcine circovirus type 2 (PCV2) infection in Hebei Province from 2016 to 2019: a retrospective study - Lei Han; Guang-fu Yuan; Shao-jie Chen; Fei Dai; Lin-shan Hou; Jing-hui Fan; Yu-zhu Zuo (Archives of Virology, 2021)

RSF1 requires CEBP/β and hSNF2H to promote IL-1β-mediated angiogenesis: the clinical and therapeutic relevance of RSF1 overexpression and amplification in myxofibrosarcomas - Chien-Feng Li; Ti-Chen Chan; Cheng-I. Wang; Fu-Min Fang; Po-Chun Lin; Shih-Chen Yu; Hsuan-Ying Huang (Angiogenesis, 2021)

Geographic and subsequent biotic isolations led to a diversity anomaly of section Heterotropa (genus Asarum : Aristolochiaceae) in insular versus continental regions of the Sino‐Japanese Floristic Region - Daiki Takahashi; Shota Sakaguchi; Yu Feng; Yuji Isagi; Ying‐Xiong Qiu; Pan Li; Rui‐Sen Lu; Chang‐Tse Lu; Shih‐Wen Chung; Yang‐Shan Lin; Yun‐Chao Chen; Atsushi J. Nagano; et al. (Journal of Biogeography, 2021)
Aim The Sino‐Japanese Floristic Region has extremely high species diversity with respect to temperate plants; however, the reasons for this diversity are poorly understood because most studies have only considered geographic isolation caused by climatic oscillations. In some plant groups, high floral trait diversity and uneven species diversity between insular systems and the continental area suggest other factors may have important roles too. The primary purpose of this study is to reveal how abiotic and biotic factors have shaped the species diversity anomaly of Heterotropa between the insular systems and the continental area. Location The Sino‐Japanese Floristic Region. Taxon Section Heterotropa (genus Asarum ; Aristolochiaceae). Methods Using ddRAD‐seq and chloroplast genome data, we built a time‐calibrated phylogenetic tree including 79 species. We estimated the patterns of floral traits (flowering time and floral size) evolution using macroevolutionary modelling, and tested the correlation of speciation rate with the trait evolution rates. Finally, we estimated the isolation factors of all taxa pairs and sister‐taxa pairs based on distribution range and floral traits. Results Phylogenetic analysis indicated that Heterotropa was diverged into two clades (continental clade and insular clade) in the Miocene, and the major subclades corresponded to geographic entities. Most rate shifts accelerating floral trait's evolution occurred during the Pleistocene period. Evolution rate of floral traits showed positive correlation with the speciation rate. Large proportion of taxa in the insular clade are distributed allopatrically. Several sister pairs showed floral trait divergence with geographic overlap. Main Conclusions The diversification of Heterotropa appears to have been triggered by geographic and climatic events, and subsequent repeated floral trait evolution with and without geographic isolation. Furthermore, the high species diversity in the insular systems would have been formed by the repeated range fragmentations and contractions. Our study demonstrates the importance of multidimensional studies to understand the diversification process of temperate plants in the Sino‐Japanese Floristic Region.

Prenylated xanthones and biphenyls from Garcinia esculenta with antistaphylococcal activity - Dan Zheng; Hong Zhang; Jia-Ming Jiang; Yu-Yu Chen; Shi-Jie Wan; Zhi-Xiu Lin; Hong-Xi Xu (Natural Product Research, 2021)

Collapsin response mediator protein 5 (CRMP5) modulates susceptibility to chronic social defeat stress in mice - Yu-Fen Lin; Kao Chin Chen; Yen Kuang Yang; Ya-Hsin Hsiao (Molecular Neurobiology, 2021)

Capacity of transportation and spread of COVID-19—an ironical fact for developed countries - Li Ma; Zhuo Yu; Yan Jiao; Lin Lin; Wei Zhong; Sara W. Day; Arnold Postlethwaite; Hong Chen; Qiang Li; Heliang Yin; Gang Wang (Environmental Science and Pollution Research, 2021)

Investigating the use of environmental tableware based on the theory of planned behavior - Jui-Che Tu; Yu-Yin Chen; Yi-Lin Lee; Xiao-Lin Wang (Environment, Development and Sustainability, 2021)

Activation of Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII) with Lidocaine Provokes Pyroptosis of Glioblastoma Cells - B. Zhou; Y. Lin; S. Chen; J. Cai; Z. Luo; S. Yu; J. Lu (Bulletin of Experimental Biology and Medicine, 2021)

Above‐ and belowground biodiversity jointly drive ecosystem stability in natural alpine grasslands on the Tibetan Plateau - Litong Chen; Lin Jiang; Xin Jing; Jiuluan Wang; Yu Shi; Haiyan Chu; Jin‐Sheng He (Global Ecology and Biogeography, 2021)
Aim Most existing studies that examined the biotic mechanisms of ecosystem stability have focused on aboveground biodiversity. Whether and how belowground biodiversity affects the stability of natural ecosystems is largely unknown. In the present study, we investigated the relationships between above‐ and belowground biodiversity and the temporal stability of grassland biomass production across a large gradient of soil and climatic conditions. Location Tibetan Plateau. Time period 2001–2016. Major taxa studied Alpine grass and soil bacteria, arbuscular mycorrhiza (AM) fungi and fauna. Methods We coupled a field biodiversity survey conducted in 54 alpine grassland ecosystems across the Tibetan Plateau with a satellite‐sensed proxy (enhanced vegetation index, EVI) of ecosystem productivity during 2001–2016. The temporal stability of primary productivity was calculated as the ratio of the mean annual peak EVI to the standard deviation of the annual peak EVI over this period. Plant diversity was determined using a classic vegetation survey approach, soil bacterial and AM fungal diversity were measured using a high‐throughput sequencing approach, and soil fauna was counted and identified at the order level after being extracted using a modified Berlese–Tullgren method. Results Our results demonstrated that both above‐ and belowground biodiversity were positively associated with ecosystem stability. Belowground biodiversity not only affected ecosystem stability directly, but also influenced it indirectly through plant diversity and soil fertility. Furthermore, soil pH, climate and its variability strongly influenced soil biodiversity, which in turn influenced ecosystem stability. Main conclusions Our study demonstrates the indispensable role of belowground biodiversity in regulating ecosystem stability, emphasizing the necessity of conserving this ‘hidden’ biodiversity in fragile alpine ecosystems facing ongoing environmental change.

Detection of Vestibular Schwannoma on Triple-parametric Magnetic Resonance Images Using Convolutional Neural Networks - Tzu-Hsuan Huang; Wei-Kai Lee; Chih-Chun Wu; Cheng-Chia Lee; Chia-Feng Lu; Huai-Che Yang; Chun-Yi Lin; Wen-Yuh Chung; Po-Shan Wang; Yen-Ling Chen; Hsiu-Mei Wu; Wan-You Guo; et al. (Journal of Medical and Biological Engineering, 2021)
Purpose The first step in typical treatment of vestibular schwannoma (VS) is to localize the tumor region, which is time-consuming and subjective because it relies on repeatedly reviewing different parametric magnetic resonance (MR) images. A reliable, automatic VS detection method can streamline the process. Methods A convolutional neural network architecture, namely YOLO-v2 with a residual network as a backbone, was used to detect VS tumors from MR images. To heighten performance, T1-weighted–contrast-enhanced, T2-weighted, and T1-weighted images were combined into triple-channel images for feature learning. The triple-channel images were cropped into three sizes to serve as input images of YOLO-v2. The VS detection effectiveness levels were evaluated for two backbone residual networks that downsampled the inputs by 16 and 32. Results The results demonstrated the VS detection capability of YOLO-v2 with a residual network as a backbone model. The average precision was 0.7953 for a model with 416 × 416-pixel input images and 16 instances of downsampling, when both the thresholds of confidence score and intersection-over-union were set to 0.5. In addition, under an appropriate threshold of confidence score, a high average precision, namely 0.8171, was attained by using a model with 448 × 448-pixel input images and 16 instances of downsampling. Conclusion We demonstrated successful VS tumor detection by using a YOLO-v2 with a residual network as a backbone model on resized triple-parametric MR images. The results indicated the influence of image size, downsampling strategy, and confidence score threshold on VS tumor detection.

Germination dynamics of allergenic fungal spores in respiratory mucus - Wan-Rou Lin; Szu-Yu Chen; Shih-Ching Hsiao; Pi-Han Wang (Aerobiologia, 2021)

MiR-124 and Small Molecules Synergistically Regulate the Generation of Neuronal Cells from Rat Cortical Reactive Astrocytes - Yangyang Zheng; Zhehao Huang; Jinying Xu; Kun Hou; Yifei Yu; Shuang Lv; Lin Chen; Yulin Li; Chengshi Quan; Guangfan Chi (Molecular Neurobiology, 2021)

Genome sequencing and functional annotation of Bacillus sp. strain BS-Z15 isolated from cotton rhizosphere soil having antagonistic activity against Verticillium dahliae - Zhong-Yi Chen; Xieerwanimu Abuduaini; Nuramina Mamat; Qi-Lin Yang; Meng-Jun Wu; Xin-Rui Lin; Ru Wang; Rong-Rong Lin; Wei-Jun Zeng; Huan-Chen Ning; He-Ping Zhao; Jin-Yu Li; et al. (Archives of Microbiology, 2021)

Termite mounds reduce soil microbial diversity by filtering rare microbial taxa - Qing‐Lin Chen; Hang‐Wei Hu; Zhen‐Zhen Yan; Chao‐Yu Li; Bao‐Anh Thi Nguyen; Yong Zheng; Yong‐Guan Zhu; Ji‐Zheng He (Environmental Microbiology, 2021)
Summary Termites are ubiquitous insects in tropical and subtropical habitats, and some of them construct massive nests (‘mounds’), which substantially promote substrate heterogeneity by altering soil properties. Yet, the role of termite nesting process in regulating the distribution and diversity of soil microbial communities remains poorly understood, which introduces uncertainty in predictions of ecosystem functions of termite mounds in a changing environment. Here, by using amplicon sequencing, we conducted a survey of 134 termite mounds across >1500 km in northern Australia and found that termite mounds significantly differed from bulk soils in the microbial diversity and community compositions. Compared with bulk soils, termite nesting process decreased the microbial diversity and the relative abundance of rare taxa. Rare taxa had a narrower habitat niche breadth than dominant taxa and might be easier to be filtered by the potential intensive microbial competition during the nesting processes. We further demonstrated that the shift in pH induced by termite nesting process was a major driver shaping the microbial community profiles in termite mounds. Together, our work provides novel evidence that termite nesting is an important process in regulating soil microbial diversity, which advances our understanding of the functioning of termite mounds.

Mortality burden caused by diurnal temperature range: a nationwide time-series study in 364 Chinese locations - Min Cai; Jianxiong Hu; Chunliang Zhou; Zhulin Hou; Yanjun Xu; Maigeng Zhou; Yize Xiao; Biao Huang; Xiaojun Xu; Lifeng Lin; Tao Liu; Jianpeng Xiao; et al. (Stochastic Environmental Research and Risk Assessment, 2021)

Human β-Defensin 118 Attenuates Escherichia coli K88–Induced Inflammation and Intestinal Injury in Mice - Qian Lin; Qingqing Fu; Xiang Li; Yuheng Luo; Junqiu Luo; Daiwen Chen; Xiangbing Mao; Bing Yu; Ping Zheng; Zhiqing Huang; Jie Yu; Hui Yan; et al. (Probiotics and Antimicrobial Proteins, 2021)

GTree: an Open-source Tool for Dense Reconstruction of Brain-wide Neuronal Population - Hang Zhou; Shiwei Li; Anan Li; Qing Huang; Feng Xiong; Ning Li; Jiacheng Han; Hongtao Kang; Yijun Chen; Yun Li; Huimin Lin; Yu-Hui Zhang; et al. (Neuroinformatics, 2021)

EEG-Based Anxious States Classification Using Affective BCI-Based Closed Neurofeedback System - Chao Chen; Xuecong Yu; Abdelkader Nasreddine Belkacem; Lin Lu; Penghai Li; Zufeng Zhang; Xiaotian Wang; Wenjun Tan; Qiang Gao; Duk Shin; Changming Wang; Sha Sha; et al. (Journal of Medical and Biological Engineering, 2021)

Calcium signalling mediated the regulation of growth and polysaccharide accumulation by light quality in Dendrobium officinale protocorms - Ting Lei; Suping Gao; Xiao Lin; Kaihui Zhang; Yingqi Liu; Wenji Li; Di Hu; Xiaofang Yu; Mingyan Jiang; Qibing Chen; Ying Sun (Horticulture, Environment, and Biotechnology, 2021)

Identification and characterization of CONSTANS-like genes from Curcuma alismatifolia - Yuan-Yuan Li; Xiao-Huang Chen; Hui-Wen Yu; Qi-Lin Tian; Luan-Mei Lu (Horticulture, Environment, and Biotechnology, 2021)

Microbiological profiles and antimicrobial resistance patterns of pediatric bloodstream pathogens in China, 2016–2018 - Yue Qiu; Junwen Yang; Yiping Chen; Jinhong Yang; Qingxiong Zhu; Chunhui Zhu; Shuangjie Li; Jing Liu; Chaomin Wan; Yu Zhu; Minxia Chen; Yi Xu; et al. (European Journal of Clinical Microbiology & Infectious Diseases, 2021)

Kinetics and mechanism of 4-methylbenzylidene camphor degradation by UV-activated persulfate oxidation - Sung-Chuan Hsieh; Webber Wei-Po Lai; Angela Yu-Chen Lin (Environmental Science and Pollution Research, 2021)

Glutathione and the intracellular labile heme pool - Rosemary O’Keeffe; Gladys Oluyemisi Latunde-Dada; Yu-Lin Chen; Xiaole L. Kong; Agostino Cilibrizzi; Robert C. Hider (BioMetals, 2021)
One candidate for the cytosolic labile iron pool is iron(II)glutathione. There is also a widely held opinion that an equivalent cytosolic labile heme pool exists and that this pool is important for the intracellular transfer of heme. Here we describe a study designed to characterise conjugates that form between heme and glutathione. In contrast to hydrated iron(II), heme reacts with glutathione, under aerobic conditions, to form the stable hematin–glutathione complex, which contains iron(III). Thus, glutathione is clearly not the cytosolic ligand for heme, indeed we demonstrate that the rate of heme degradation is enhanced in the presence of glutathione. We suggest that the concentration of heme in the cytosol is extremely low and that intracellular heme transfer occurs via intracellular membrane structures. Should any heme inadvertently escape into the cytosol, it would be rapidly conjugated to glutathione thereby protecting the cell from the toxic effects of heme.

miR-23b Attenuates LPS-Induced Inflammatory Responses in Acute Lung Injury via Inhibition of HDAC2 - Zhi-Feng Luo; Xiang-Hui Jiang; Huan Liu; Li-Yuan He; Xiong Luo; Fu-Chun Chen; Yu-Lin Tan (Biochemical Genetics, 2021)

The Effect of Wave-Induced Current and Coastal Structure on Sediment Transport at the Zengwen River Mouth - Chun-Hung Pao; Jia-Lin Chen; Shih-Feng Su; Yu-Ching Huang; Wen-Hsin Huang; Chien-Hung Kuo (Journal of Marine Science and Engineering, 2021)
The mechanisms that control estuarine sediment transport are complicated due to the interaction between riverine flows, tidal currents, waves, and wave-driven currents. In the past decade, severe seabed erosion and shoreline retreat along the sandy coast of western Taiwan have raised concerns regarding the sustainability of coastal structures. In this study, ADCPs (Acoustic Doppler Current Profiler) and turbidity meters were deployed at the mouth of the Zengwen river to obtain the time series and the spatial distribution of flow velocities and turbidity during the base flow and flood conditions. A nearshore circulation model, SHORECIRC, has been adapted into a hybrid finite-difference/finite-volume, TVD (Total Variation Diminishing)-type scheme and coupled with the wave-spectrum model Simulating Waves Nearshore (SWAN). Conventional finite-difference schemes often produce unphysical oscillations when modeling coastal processes with abrupt bathymetric changes at river mouths. In contrast, the TVD-type finite volume scheme allows for robust treatment of discontinuities through the shock-capturing mechanism. The model reproduces water levels, waves, currents observed at the mouth of the Zengwen River reasonably well. The simulated residual sediment transport patterns demonstrate that the transport process at the river mouth is dominated by the interaction of the bathymetry and wave-induced currents when the riverine discharge was kept in reservoirs. The offshore residual transport causes erosion at the northern part of the river mouth, and the onshore residual transport causes accretion in the ebb tidal shoals around the center of the river mouth. The simulated morphological evolution displays significant changes on shallower deltas. The location with significant sea bed changes is consistent with the spot in which severe erosion occurred in recent years. Further analysis of morphological evolution is also discussed to identify the role of coastal structures, for example, the extension of the newly constructed groins near the river mouth.

Natural polyketide 6-pentyl-2 H -pyrone-2-one and its synthetic analogues efficiently prevent marine biofouling - Mo Aqib Raza Khan; Bo-Wei Wang; Yih-Yu Chen; Ting-Hsuan Lin; Hsiu-Chin Lin; Yu-Liang Yang; Ka-Lai Pang; Chih-Chuang Liaw (Biofouling, 2021)

Impacts of sea level rise on future storm-induced coastal inundations over massachusetts coast - Changsheng Chen; Zhaolin Lin; Robert C. Beardsley; Tom Shyka; Yu Zhang; Qichun Xu; Jianhua Qi; Huichan Lin; Danya Xu (Natural Hazards, 2021)

Kidney function change after transcatheter aortic valve replacement in patients with diabetes and/or hypertension - Jiaqi Fan; Changjie Yu; Kaida Ren; Wanbing Lin; Stella Ng; Zexin Chen; Xinping Lin; Lihan Wang; Qifeng Zhu; Yuxin He; Jubo Jiang; Xianbao Liu; et al. (Journal of Zhejiang University-SCIENCE B, 2021)

Syndecan-3 contributes to the regulation of the microenvironment at the node of Ranvier following end-to‑side neurorrhaphy: sodium image analysis - Chiung-Hui Liu; Yu-Chen Kuo; Che-Yu Wang; Chao-Chun Hsu; Ying-Jui Ho; Yun-Chi Chiang; Fu-Der Mai; Wei-Jhih Lin; Wen-Chieh Liao (Histochemistry and Cell Biology, 2021)

RING-finger protein 5 promotes hepatocellular carcinoma progression and predicts poor prognosis - Yi Zhang; Jie Li; Huayang Chen; Changmao Zhang; Song You; Yue Zhao; Xiaoning Lin; Yaqi Yu; Fei Fang; Ting Fang; Xiaomin Wang (Human Cell, 2021)

Delayed association of acute particulate matter 2.5 air pollution exposure with loss of complexity in cardiac rhythm dynamics: insight from detrended fluctuation analysis - Tsung-Ying Tsai; Li-Wei Lo; Shin-Huei Liu; Wen-Han Cheng; Yu-Hui Chou; Wei-Lun Lin; Yenn-Jiang Lin; Shih-Lin Chang; Yu-Feng Hu; Fa-Po Chung; Jo-Nan Liao; Tze-Fan Chao; et al. (Environmental Science and Pollution Research, 2021)

Remediation of contaminated soil and groundwater using chemical reduction and solidification/stabilization method: a case study - Shi-Feng Lu; Yu-Lin Wu; Zhan Chen; Tao Li; Chao Shen; Lin-Kang Xuan; Ling Xu (Environmental Science and Pollution Research, 2021)

Description of the reservoir along strike-slip fault zones in China T-Sh oilfield, Tarim Basin - Rui Zhao; Shang Deng; Lu Yun; Huixi Lin; Teng Zhao; Chen Yu; Qiangfu Kong; Qiang Wang; Huili Li (Carbonates and Evaporites, 2021)

Continental shelf morphology controlled by bottom currents, mud diapirism, and submarine slumping to the east of the Gaoping Canyon, off SW Taiwan - Yi-Ching Yeh; Chung-Lin Tsai; Shu-Kun Hsu; Hsiao-Shan Lin; Kuan-Ting Chen; Yen-Yu Cho; Chin-Wei Liang (Geo-Marine Letters, 2021)

Cu2+ Inhibits the Peroxidase and Antibacterial Activity of Homodimer Hemoglobin From Blood Clam Tegillarca granosa by Destroying Its Heme Pocket Structure - Sufang Wang; Xiaopei Yu; Shunqin Zhang; Hongyu Jin; Zhongfa Chen; Zhihua Lin; Yongbo Bao (Frontiers in Marine Science, 2021)
Beyond its role as an oxygen transport protein, the homodimer hemoglobin of blood clam Tegillarca granosa (Tg-HbI) has been found to possess antibacterial activity. However, the mechanism of antibacterial activity of Tg-HbI remain to be investigated. In this study, we investigated the effects of Cu 2+ on the structure, peroxidase activity, and antibacterial ability of Tg-HbI. Tg-HbI was significantly inactivated by Cu 2+ in a non-competitive inhibition manner, following first-order reaction kinetics. The Spectroscopy results showed that Cu 2+ changed the iron porphyrin ring and the coordination of heme with proximal histidine of Tg-HbI, and increased the hydrophobicity of heme pocket. We found that proline could stabilize the heme pocket structure of Tg-HbI, hence, protect peroxidase activity and antimicrobial activity of Tg-HbI against damage by Cu 2+ . Our results suggest that Cu 2+ inhibits the peroxidase and antibacterial activity of Tg-HbI by destroying its heme pocket structure and Tg-HbI probably plays an antibacterial role through its peroxidase activity. This result could provide insights into the antibacterial mechanism of Tg-HbI.

First report of turnip mosaic virus on yellow passion fruit in China - Li Juan Chen; Xing Xing Zhang; Ying Lin Lu; Yu Xing An (Journal of Plant Pathology, 2021)

Injection route affects intra-articular hyaluronic acid distribution and clinical outcome in viscosupplementation treatment for knee osteoarthritis: a combined cadaver study and randomized clinical trial - Jun Xiao; Yong Hu; Lin Huang; Zhi-Fa Huang; Wei-Zhou Jiang; Yu-Qiang Luo; Meng-Yan Jia; Di Chen; Zhan-Jun Shi (Drug Delivery and Translational Research, 2021)

Associations of Serum Selenium Levels in the First Trimester of Pregnancy with the Risk of Gestational Diabetes Mellitus and Preterm Birth: a Preliminary Cohort Study - Peng Ju Liu; Aimin Yao; Liangkun Ma; Xiao Yan Chen; Song Lin Yu; Yanping Liu; Yi Xuan Hou (Biological Trace Element Research, 2021)

Comparative Evaluation on Combined Ecotoxic Effects of Nitrofurazone and NH4CI Using Two Ciliated Protists as Model Organisms: Population Dynamics and Growth Rates - Ziyue Yu; Shuxing Liu; Xin Chen; Alan Warren; Xiaofeng Lin; Jiqiu Li (Journal of Ocean University of China, 2021)

Dapagliflozin suppresses ER stress and protects doxorubicin-induced cardiotoxicity in breast cancer patients - Wei-Ting Chang; Yu-Wen Lin; Chung-Han Ho; Zhih-Cherng Chen; Ping-Yen Liu; Jhih-Yuan Shih (Archives of Toxicology, 2021)

A drug-tunable Flt23k gene therapy for controlled intervention in retinal neovascularization - Jinying Chen; Fan-Li Lin; Jacqueline Y. K. Leung; Leilei Tu; Jiang-Hui Wang; Yu-Fan Chuang; Fan Li; Hsin-Hui Shen; Gregory J. Dusting; Vickie H. Y. Wong; Leszek Lisowski; Alex W. Hewitt; et al. (Angiogenesis, 2021)

Datasets for the CMIP6 Scenario Model Intercomparison Project (ScenarioMIP) Simulations with the Coupled Model CAS FGOALS-f3-L - Shuwen Zhao; Yongqiang Yu; Pengfei Lin; Hailong Liu; Bian He; Qing Bao; Yuyang Guo; Lijuan Hua; Kangjun Chen; Xiaowei Wang (Advances in Atmospheric Sciences, 2021)
The datasets for the tier-1 Scenario Model Intercomparison Project (ScenarioMIP) experiments from the Chinese Academy of Sciences (CAS) Flexible Global Ocean-Atmosphere-Land System model, finite-volume version 3 (CAS FGOALS-f3-L) are described in this study. ScenarioMIP is one of the core MIP experiments in phase 6 of the Coupled Model Intercomparison Project (CMIP6). Considering future CO 2 , CH 4 , N 2 O and other gases’ concentrations, as well as land use, the design of ScenarioMIP involves eight pathways, including two tiers (tier-1 and tier-2) of priority. Tier-1 includes four combined Shared Socioeconomic Pathways (SSPs) with radiative forcing, i.e., SSP1-2.6, SSP2-4.5, SSP3-7.0 and SSP5-8.5, in which the globally averaged radiative forcing at the top of the atmosphere around the year 2100 is approximately 2.6, 4.5, 7.0 and 8.5 W m −2 , respectively. This study provides an introduction to the ScenarioMIP datasets of this model, such as their storage location, sizes, variables, etc. Preliminary analysis indicates that surface air temperatures will increase by about 1.89°C, 3.07°C, 4.06°C and 5.17°C by around 2100 under these four scenarios, respectively. Meanwhile, some other key climate variables, such as sea-ice extension, precipitation, heat content, and sea level rise, also show significant long-term trends associated with the radiative forcing increases. These datasets will help us understand how the climate will change under different anthropogenic and radiative forcings.

CAS-ESM2.0 Model Datasets for the CMIP6 Ocean Model Intercomparison Project Phase 1 (OMIP1) - Xiao Dong; Jiangbo Jin; Hailong Liu; He Zhang; Minghua Zhang; Pengfei Lin; Qingcun Zeng; Guangqing Zhou; Yongqiang Yu; Mirong Song; Zhaohui Lin; Ruxu Lian; et al. (Advances in Atmospheric Sciences, 2021)
As a member of the Chinese modeling groups, the coupled ocean-ice component of the Chinese Academy of Sciences’ Earth System Model, version 2.0 (CAS-ESM2.0), is taking part in the Ocean Model Intercomparison Project Phase 1 (OMIP1) experiment of phase 6 of the Coupled Model Intercomparison Project (CMIP6). The simulation was conducted, and monthly outputs have been published on the ESGF (Earth System Grid Federation) data server. In this paper, the experimental dataset is introduced, and the preliminary performances of the ocean model in simulating the global ocean temperature, salinity, sea surface temperature, sea surface salinity, sea surface height, sea ice, and Atlantic Meridional Overturning Circulation (AMOC) are evaluated. The results show that the model is at quasi-equilibrium during the integration of 372 years, and performances of the model are reasonable compared with observations. This dataset is ready to be downloaded and used by the community in related research, e.g., multi-ocean-sea-ice model performance evaluation and interannual variation in oceans driven by prescribed atmospheric forcing.

Highly enriched N‐containing organic molecules of Synechococcus lysates and their rapid transformation by heterotrophic bacteria - Qiang Zheng; Wenxin Lin; Yu Wang; Yunyun Li; Chen He; Yuan Shen; Weidong Guo; Quan Shi; Nianzhi Jiao (Limnology and Oceanography, 2021)
Phytoplankton are major contributors of labile dissolved organic matter to marine euphotic zone ecosystems. Viral‐mediated phytoplankton lysis releases a considerable amount of bioavailable cellular contents and facilitates localized heterotrophic bacterial growth. The rapid turnover times of this labile organic matter renders it difficult to trace their transformation within natural environments. In the present study, we used a model phototroph‐heterotroph coculture system to investigate the organic molecular variation during cyanophage‐induced Synechococcus lysis and post‐lysis periods using fluorescence spectroscopy and ultrahigh resolution mass spectrometry analyses. Approximately 80% of the Synechococcus lysates were respired to carbon dioxide within 2 days coupled to rapid regeneration of inorganic nutrients. Five fluorescent dissolved organic matter components were detected in the incubations including four humic‐like and one protein‐like components, and their abundances varied markedly with the succession of the viral lysis process. Viral lysis promoted the transformation of Synechococcus biomass to dissolved organic matter, and heterotrophic bacterial metabolism facilitated the transformation of organic molecules from relatively high (avg. ~ 430 Da) to low (avg. ~ 360 Da) molecular weight, corresponding to variation in their bioavailability. The Synechococcus ‐derived organic matter was highly enriched in N‐containing organic molecules. Identified metabolites within the cultures primarily comprised amino acids or oligopeptides and other low‐molecular‐weight organic acids, carbohydrates, nucleotides, lipids, biogenic amines, and porphyrins. Among these, oligopeptides, nucleotides, and lipid compounds significantly increased with viral lysis. These results provide insight into biogeochemical cycling mediated by viral shunts in oceans, thus deepening our understanding of microbial food webs at the molecular level.

Experimental evidence for long‐term coexistence of copiotrophic and oligotrophic bacteria in pelagic surface seawater - Yi Li; Zhi‐Bin Wang; Xi‐Ying Zhang; Yan‐Ru Dang; Lin‐Lin Sun; Wei‐Peng Zhang; Hui‐Hui Fu; Gui‐Peng Yang; Min Wang; Andrew McMinn; Xiu‐Lan Chen; Yin Chen; et al. (Environmental Microbiology, 2021)
Summary Most marine copiotrophic bacteria can produce extracellular enzymes to degrade biopolymers into bio‐available smaller solutes, while oligotrophic bacteria usually cannot. Bacterial extracellular enzymes and enzymatic products can be a common resource that could be utilized by both copiotrophs and oligotrophs; when present, oligotrophs may outcompete the enzyme‐producing copiotrophs. However, copiotrophs and oligotrophs consistently coexist in the ocean. How they maintain coexistence has still not been experimentally studied. In this study, the interaction and coexistence of a copiotroph and an oligotroph, isolated from the same surface seawater sample and utilizing the same proteinaceous substrate, were experimentally investigated. The copiotroph could secrete extracellular proteases to degrade and then utilize the proteinaceous substrate. The oligotroph was unable to utilize the proteinaceous substrate by itself, but could grow by using the hydrolysate amino acids. The copiotroph outcompeted the oligotroph by adsorbing the amino acids quickly and having a higher growth rate in the rich medium. The oligotroph survived by adapting to low concentration of nutrients. The copiotroph and oligotroph were able to maintain long‐term (up to 142 days) coexistence in the laboratory. This study indicates that differences in the utilization of different concentrations of nutrients can drive the coexistence of marine copiotrophs and oligotrophs.

Understanding the dynamical-microphysical-electrical processes associated with severe thunderstorms over the Beijing metropolitan region - Xiushu Qie; Shanfeng Yuan; Zhixiong Chen; Dongfang Wang; Dongxia Liu; Mengyu Sun; Zhuling Sun; Abhay Srivastava; Hongbo Zhang; Jingyu Lu; Hui Xiao; Yongheng Bi; et al. (Science China Earth Sciences, 2021)

Identification of 5S and 45S rDNA sites in Chrysanthemum species by using oligonucleotide fluorescence in situ hybridization (Oligo-FISH) - Jun He; Sisi Lin; Zhongyu Yu; Aiping Song; Zhiyong Guan; Weimin Fang; Sumei Chen; Fei Zhang; Jiafu Jiang; Fadi Chen; Haibin Wang (Molecular Biology Reports, 2021)

A plant virus mediates interspecific competition between its insect vectors in Capsicuum annuum - Yong Chen; Xue Zheng; Hui Wei; Yong-Dui Chen; Kuan-Yu Zheng; Ye Mu; Xing-Yue Zhao; Li-Hua Zhao; Yu-Lin Gao; Li-Min Zheng; Jie Zhang (Journal of Pest Science, 2021)

Improved control of Frankliniella occidentalis on greenhouse pepper through the integration of Orius sauteri and neonicotinoid insecticides - Qing-cai Lin; Hao Chen; Dirk Babendreier; Jin-ping Zhang; Feng Zhang; Xiao-yan Dai; Zuo-wen Sun; Zhao-peng Shi; Xiao-lin Dong; Guang-an Wu; Yi Yu; Li Zheng; et al. (Journal of Pest Science, 2021)

Geographical and interspecific variation in susceptibility of three common thrips species to the insecticide, spinetoram - Yong-Fu Gao; Ya-Jun Gong; Li-Jun Cao; Jin-Cui Chen; Yu-Lin Gao; Majid Mirab-balou; Min Chen; Ary A. Hoffmann; Shu-Jun Wei (Journal of Pest Science, 2021)

Heterosis-associated genes confer high yield in super hybrid rice - Tianzi Lin; Cong Zhou; Gaoming Chen; Jun Yu; Wei Wu; Yuwei Ge; Xiaolan Liu; Jin Li; Xingzhou Jiang; Weijie Tang; Yunlu Tian; Zhigang Zhao; et al. (Theoretical and Applied Genetics, 2020)
Key message Heterosis QTLs, including qSS7 and qHD8, with dominance effects were identified through GBS and large-scale phenotyping of CSSLs and hybrid F 1 populations in a paddy field. Abstract Heterosis has contributed immensely to agricultural production, but its genetic basis is unclear. We evaluated dominance effects by creating two hybrid populations: a B-homo set with a homozygous background and heterozygous chromosomal segments and a B-heter set with a heterozygous background and homozygous segments. This was achieved by crossing a set of 156 backcrossed-derived chromosome segment substitution lines (CSSLs) with their recurrent parent (9311), the male parent of the first super-high-yield hybrid Liangyoupei9 (LYP9), and with the female parent (PA64s) of the hybrid. The CSSLs were subjected to a genotyping-by-sequencing analysis to develop a genetic map of segments introduced from the PA64s. We evaluated the heterotic effects on eight yield-related traits in the hybrid variety and F 1 populations in large-scale field experiments over 2 years. Using a linkage map consisting of high-density SNPs, we identified heterosis-associated genes in LYP9. Five candidate genes contributed to the high yield of LYP9, with qSS7 and qHD8 repeatedly detected in both B-hybrid populations. The heterozygous segments harboring qSS7 and qHD8 showed dominance effects that contributed to the heterosis of yield components in the hybrid rice variety Liangyoupei9.

Concurrent increases in post-pacing action potential duration and contractility predict occurrence of ventricular arrhythmia - Chih-Min Liu; Feng-Zhi Lin; Yao-Chang Chen; Yung-Kuo Lin; Yen-Yu Lu; Cheng-I Wu; Satoshi Higa; Shih-Ann Chen; Yi-Jen Chen (Pflügers Archiv - European Journal of Physiology, 2020)

Improving Image Correlation and Differentiation of 3D Endoluminal Lesions in the Air Spaces Using a Novel Target Gray Level Mapping Technique: A Preliminary Study of Its Application to Computed Tomographic Colonography and Comparison with Traditional Surface Rendering Method - Lih-Shyang Chen; Ta-Wen Hsu; Shao-Jer Chen; Shu-Han Chang; Chih-Wen Lin; Yu-Ruei Chen; Chin-Chiang Hsieh; Shu-Chen Han; Ku-Yaw Chang; Chun-Ju Hou (Journal of Medical and Biological Engineering, 2020)