Biodegradation of short-chain phthalate esters (PAEs) by Sinomonas sp. S2 isolated from a soil contaminated by metal(loid)s smelting - Pengfei Shao; Jun Yao; Miaomiao Li; Ning Min (Biodegradation, 2025)

Heterotrophic nitrification-aerobic denitrification characteristics and zinc-containing wastewater treatment potential of pseudomonas hunanensis SK-4: screening, application, and mechanistic insights - Yuhong Zhang; Jiacheng Cai; Jiabao Wang; Yueqing Jiang (Biodegradation, 2025)

Dual-function strategy: simultaneous biopolymer production and lead biosorption by Bacillus paramycoides in single-batch fermentation process - Raghavendra Paduvari; Roopashri Arekal; Divyashree Mysore Somashekara (Biodegradation, 2025)
The conventional physicochemical processes of lead removal from contaminated wastewater are cost-intensive and generate toxic secondary wastes. Therefore, employing bacteria capable of lead biosorption will serve as a green, sustainable, safe and inexpensive process for lead removal, and its integration with polyhydroxyalkanoate (PHA) production will generate revenue. To integrate these two processes, Bacillus paramycoides isolated from the lake water were analyzed for lead biosorption and PHA production in mineral salt media containing lead nitrate at different incubation times. The bacterium showed a high lead biosorption of about 423 mg/g of biomass at 72 h of incubation, which was earlier not reported. Bacillus paramycoides produced polyhydroxybutyrate by utilizing sucrose in the media. The lead ions enhanced the PHA production up to 35.7% CDW in bacterial cells by inducing oxidative stress, and the ability of PHA polymer to adsorb lead ions prevented further oxidative damage to the cells. This study is the first report on using an integrated process approach for lead biosorption and PHA production in bacteria.

Production and characterization of Laccase from Lentinus berteroi and applications for biodegradation of organic micropollutants - Katielle Vieira Avelino; Marisangela Isabel Wietzikoski Halabura; Edileusa Cristina Marques Gerhardt; Maira Gabriela Paetzold; Asif Khan; Juliana Silveira do Valle; Flavio Augusto Vicente Seixas (Biodegradation, 2025)

Heavy metal pollution assessment and bioremediation potential of multidrug-resistant Proteus mirabilis isolated from Buckingham canal, Chennai - S. Sugitha; G. Abirami (Biodegradation, 2025)

Bioremediation of methotrexate by Pleurotus ostreatus pellets: effects on morphology and biotransformation insights - Thabata Montserrat Hernández-Cruz; Karina García-Gutiérrez; Adriana Jazmín Legorreta-Castañeda; Guadalupe Guerra-Sánchez; Dario Rafael Olicón-Hernández (Biodegradation, 2025)

Harnessing SDS as a source of sulfur: a bioremediation strategy of Fischerella sp. lmga1 - Ankit Srivastava; Anirbana Parida; Samujjal Bhattacharjee; Neha Gupta; Satya Shila Singh; Arun Kumar Mishra (Biodegradation, 2025)

Biotransformation of As, Cr, Hg, and Mn by Pseudomonadota: chances and risks - Aleksandra Naziębło; Jakub Dobrzyński (Biodegradation, 2025)
Heavy metals are among the key environmental contaminants which pose a threat to all the living organisms. Bacteria can help detoxify these elements by a range of mechanisms associated to bacterial metabolic processes. One of them is biotransformation, which consists in change in the oxidative state of an element, often followed by its absorption or precipitation. Pseudomonadota—a vast and diverse bacterial phylum—belong to the best studied microorganisms involved in the process. Our review shows their contribution to the redox-based detoxification of four trace elements: chromium, manganese, mercury, and arsenic. Based on a comprehensive literature survey, we try to assess the importance of individual orders and selected genera in redox transformations; we also identify potential risks associated with the use of Pseudomonadota in bioremediation and suggest the most promising directions for future studies and applications. The review leads to a conclusion that the potential of non-pathogenic rhizobia Hyphomicrobiales is particularly worth further exploration.

Correction: Physiological and transcriptomic response of enriched anammox culture upon elevated hydrazine exposure - Tugba Sari; Kozet Yapsakli; Deniz Akgul; Bulent Mertoglu (Biodegradation, 2025)

Chitin degradation by the aquatic pigment-producing bacteria of Janthinobacterium spp. from stream flows and springs of Shulgan River basin and Shulgan-Tash Cave, Southern Urals - Gleb E. Aktuganov; Elena A. Gilvanova; Polina Yu. Milman; Ravil R. Garafutdinov; Alena S. Ryabova; Nailya F. Galimzianova; Olga Ya. Chervyatsova; Lyudmila Yu. Kuzmina (Biodegradation, 2025)

Bioconversion of food waste to poly (3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer using Stenotrophomonas geniculata: study of operating parameters and process optimization - Hanieh Karimnezhad; Farshad Rahimpour; Sara Samadi (Biodegradation, 2025)

Microbiota and methanogenic activities in an anaerobic internal circulation reactor: insights into biogas production from brewery wastewater - Cecilia Callejas; Leandro Guerrero; Leonardo Erijman; Iván López; Liliana Borzacconi (Biodegradation, 2025)

Machine learning-based optimization of biogas and methane yields in UASB reactors for treating domestic wastewater - Saurabh Kumar; Saurabh Kumar; Divesh Ranjan Kumar; Dayanand Sharma; Warit Wipulanusat (Biodegradation, 2025)

Utilization of the C9 aromatic hydrocarbon n-propylbenzene by Sphingobium barthaii KK22 and coexistence of multiple biodegradation pathways - Miharu Sakai; Jiro F. Mori; Robert A. Kanaly (Biodegradation, 2025)

Biodegradation of pretilachlor and butachlor by novel bacterial strains isolated from paddy field soil - Nguyen Thi Oanh; Ha Danh Duc (Biodegradation, 2025)

Bioremediation of Cr(VI) using novel thermophilic bacteria Brevibacillus borstelensis SSAU-3 T: optimization, mechanism and phytotoxicity study - Manshi Agrawal; Abhijeet Sharma; Akanksha Singh; Shanthy Sundaram (Biodegradation, 2025)

Mycotransformation of anthracene by indigenous Trichoderma lixii and Talaromyces pinophilus isolates: insights into the metabolic pathways, enzyme profiles and acute toxicity - Samson O. Egbewale; Ajit Kumar; Mduduzi P. Mokoena; Ademola O. Olaniran (Biodegradation, 2025)
This study focused on the mycotransformation of a very prominent PAH, anthracene, and its acute toxicity reduction by Ascomycete fungi: Trichoderma lixii strain FLU1 ( Tl FLU1) and Talaromyces pinophilus strain FLU12 ( Tp FLU12), indigenously isolated from benzo[b] fluoranthene-enriched activated sludge. The results indicate that both the isolates Tl FLU1 and Tp FLU12 could tolerate anthracene exposure up to 1000 mg/L, with increased expression of ligninolytic enzymes: Laccase, Lignin peroxidase, and Manganese peroxidase. The mycotransformation of anthracene was observed to be growth-linked and mediated by the expression of the intracellular enzymes as the initial mechanism used by these strains followed by the ligninolytic enzymes with up to 56% and 38% anthracene degradation by Tl FLU1 and Tp FLU12, respectively, after 24 days with a concomitant change in pH from 5 to 4 ( Tl FLU1) and 6.2 ( Tp FLU12). The GC–MS and FTIR analysis of the samples indicate the appearance of metabolic intermediates: 9,10 anthracenedione and benzoic acid in Tl FLU1 grown medium, while anthrone and 9,10 anthracenedione were detected in Tp FLU12 grown medium. The mycotransformation of the compound followed a first-order kinetic model with an effective concentration (EC 50 ) of 262.3–266.1 mg/L, with a toxicity unit (TU) of 0.4% in Vibrio parahaemolyticus (6 h exposure) to each intermediate. Results show efficient mycotransformation of anthracene into a non-toxic state by Tl FLU1 and Tp FLU12.

Influence of pH on the biodegradation efficiency of fats, oils, and grease by biosurfactant-producing bacterial consortia - Jia-Ying Wong; Ngui-Sing Ngieng; Ahmad Husaini; Rosmawati Saat; Hasnain Hussain (Biodegradation, 2025)

Pesticide degradation capacity of a novel strain belonging to Serratia sarumanii with its genomic profile - Gülperi Alatassi; Ömür Baysal; Ragıp Soner Silme; Gülçin Pınar Örnek; Hakan Örnek; Ahmet Can (Biodegradation, 2025)
The extensive use of pesticides in agricultural practices, coupled with the potential for microbial biodegradation of these chemicals, plays a critical role in environmental sustainability. This study aimed to identify microorganisms capable of degrading the most commonly used pesticides in agricultural fields within our region. In vitro screening revealed a microorganism with a broad pesticide degradation spectrum, and whole-genome sequencing further indicated the presence of genomic regions associated with pesticide degradation, a finding that was validated by LC–MS/MS analysis. Detailed genomic analysis, including ribosomal multi-locus sequence typing (rMLST), identified the microorganism as Serratia sarumanii . Our results also demonstrated that the introduction of this strain into the environment not only promoted the degradation of specific pesticides but also enhanced the efficacy of certain other pesticides at low concentrations through a synergistic interaction. To further substantiate the biodegradation capabilities of the strain, LC–MS/MS chromatographic analysis of 25 pesticide-active chemicals confirmed that Serratia sarumanii effectively biodegrades several pesticide active ingredients, including fludioxonil, fenhexamid, pyrimethanil, and spirodiclofen. These findings underscore the biodegradative potential of Serratia sarumanii and its promising application in the bioremediation of pesticide-contaminated soils. Graphical abstract

A review of enhancement of chlorophenol bioremediation using synergistic effects between zero-valent iron and microorganisms - Hao Liu; Deli Wu; Weishi Wang (Biodegradation, 2025)

Green treatments for polyaromatic hydrocarbons in e-wastes - Bandita Dutta; Debarati Chatterjee; Arina Guha; Rina Rani Ray (Biodegradation, 2025)

Biodegradation assessment tests of biopolymers in standardised water: different sources of variability - David Gutiérrez-Rial; Iria Villar; Pilar Feijóo; Benedicto Soto; Josefina Garrido; Salustiano Mato (Biodegradation, 2025)
This study assessed the ultimate biodegradation degree of two resins, polyhydroxybutyrate and polylactic acid (PHB and PLA), and three commercial biobased bags (BMAT, BGREEN, and BBEIGE) through the measurement of oxygen consumption in closed respirometers. Activated sludge from a wastewater treatment plant (WWTP) was used as the inoculum, cellulose was used as the reference material, and five trials were conducted with two different devices under identical conditions, with a 28-day incubation period. The results revealed statistically significant differences in the biochemical oxygen demand (BOD) measurements for cellulose, PHB, and PLA between the two devices and within the same devices across different trials. The degree of biodegradation (D t ), calculated as the percentage of theoretical oxygen demand (ThOD), varied depending on the device and trial. For cellulose, D t ranged from 61 to 93%; for PLA, the maximum Dt was 6%; and for PHB, D t oscillated between 16 and 72%. These findings highlight the critical importance of carefully selecting the testing equipment, as it significantly influences biodegradation results, in addition to the already known interlaboratory variability caused by the inoculum.

Enzymatic degradation of PET by hydrolase from Brucella intermedia IITR130 and its genomic insights - Pallavi Srivastava; Saurabh Singh; Mohini Soni; J. Venkatesh Pratap; Srikrishna Subramanian; Natesan Manickam (Biodegradation, 2025)

Kinetic behaviour of petroleum based oily wastewater treatment in a hybrid moving bed biofilm reactor - P. Sanghamitra; Debabrata Mazumder; Somnath Mukherjee (Biodegradation, 2025)

Achromobacter xylosoxidans and Stutzerimonas stutzeri from the phyllosphere of Eichhornia crassipes (water hyacinth) degrade chlorpyrifos as consortia - Sheeba Hoda; Kamal Krishan Aggarwal (Biodegradation, 2025)

The performance of hybrid materials for the biodegradation of dichloromethane using Pseudomonas aeruginosa, coconut shell, rice husk, and metal organic framework - Anju Mangotra (Biodegradation, 2025)

Pharmaceutical micropollutants removal and N2O production by nitrification process in SBR and SBBR: a review - J. Leiva-González; L. Hernández-Vélez; J. Quezada-Cáceres; J. Pagés-Diaz; C. Huiliñir (Biodegradation, 2025)

Physiological and transcriptomic response of enriched anammox culture upon elevated hydrazine exposure - Tugba Sari; Kozet Yapsakli; Deniz Akgul; Bulent Mertoglu (Biodegradation, 2025)
Anammox has emerged as a cost-effective and eco-friendly biological treatment technology for high-strength wastewater, and hydrazine (N 2 H 4 ) is a unique intermediate in the anammox metabolism. This study presents the first investigation into the genetic responses of anammox bacteria to elevated N 2 H 4 concentrations, offering critical insights into their potential for sustainable environmental applications. In this scope, anammox cultures were exposed to high levels of N 2 H 4 (up to 3 g/L) over a short-term period to evaluate their nitrogen treatment capacity and transcriptional responses. The results indicated that anammox activity continued at N 2 H 4 concentrations of 1.88 g/L or less. However, acute N 2 H 4 exposure significantly downregulated key genes, such as acetyl-CoA synthase beta and delta subunits, hydrazine synthase, hydrazine dehydrogenase, and hydroxylamine oxidoreductase, except for AAA family ATPase. Overall, high exogenous N 2 H 4 concentrations severely constrained the metabolism and survival of anammox bacteria at a molecular level. Understanding the genetic responses of anammox bacteria to elevated N 2 H 4 concentrations is crucial for optimizing their application in further anammox-based technologies. Future studies should focus on improving the resilience of anammox bacteria to high N 2 H 4 concentrations, thereby broadening their applicability in engineered wastewater treatment and biotechnological processes while maintaining system stability and efficiency.

Removal of high concentrations of zinc, cadmium, and nickel heavy metals by Bacillus and Comamonas through microbially induced carbonate precipitation - Adharsh Rajasekar; Cailin Zhao; Suowei Wu; Raphinos Tackmore Murava; Eyram Norgbey; Armstrong Ighodalo Omoregie; Charles K. S. Moy (Biodegradation, 2025)
Heavy metal pollution in urban freshwater, driven by anthropogenic activities, poses significant risks to aquatic ecosystems and human health due to its toxicity and persistence. Recently, urease-producing bacteria have gained attention for their ability to remove heavy metals through microbial-induced carbonate precipitation (MICP). In this study, eight urease-producing bacteria were exposed to individual solutions of zinc (Zn 2+ ), cadmium (Cd 2+ ), and nickel (Ni 2+ ) at concentrations ranging from 0 to 6 mM to assess their resistance. Three strains— Bacillus subtilis HMZC1 (B2), Bacillus sp. HMZCSW (B6), and Comamonas sp. HMZC (B11)—survived at 4 mM and 6 mM, while most others could not tolerate 4 mM. Their urea-degrading ability was tested at different pH levels, identifying an optimal pH of 7 for MICP. Heavy metal carbonate precipitation experiments at 4 mM and 6 mM revealed that all three strains achieved > 93% removal of Zn 2+ , Ni 2+ , and Cd 2+ within 72 h. Comamonas sp. HMZC exhibited the highest efficiency, achieving > 95% removal of certain heavy metals at 6 mM. Statistical analysis using one-way ANOVA revealed significant differences (p 2+ and Zn 2+ at 4 mM), although not all comparisons reached statistical significance. Scanning Electron Microscopy and X-ray Diffraction confirmed the morphology and composition of the precipitated heavy metal carbonates. Our findings demonstrate that urease-producing bacteria can effectively immobilize multiple heavy metals, highlighting the MICP process as a practical and sustainable biological approach for ecological restoration and wastewater treatment.

Adsorption of residual methyl green by extracellular polymeric substance of Lysinibacillus sp. SS1: A sustainable approach to wastewater treatment - Sneha Nayak; Louella Concepta Goveas (Biodegradation, 2025)

Assessing the exposure of lead, cadmium, and arsenic on growth parameters and antioxidant defense system in wheat - Alliluev Ilya; Irshad Ahmad; Krepakova Maria Romanovna; Tatiana Minkina; Chernikova Natalya Petrovna; Mandzhieva Saglara; Vishnu D. Rajput; Boldyreva Veronika Eduardovna; Barakhov Anatoly Vadimovich; Chaplygin Viktor Anatolievich (Biodegradation, 2025)

Inclusion of fly ash effects in ADM1: calibration and validation of a simple function using real wastewater - Gustavo Vargas-Morales; Yeney Lauzurique; Cesar Huiliñir (Biodegradation, 2025)

An exploration of bacterial consortia in chlorpyrifos degradation, soil remediation, and promotion of plant growth - Ranu Yadav; Santosh Kumar Yadav; Disha Mishra; Puja Khare (Biodegradation, 2025)

Development of a robust enzyme cascade system: co-immobilization of laccase and versatile peroxidase on polyacrylamide hydrogel for enhanced BPA degradation - Shagufta Kamal; Taleeha Roheen; Kanwal Rehman; Ismat Bibi; Muhammad Sajid Hamid Akash (Biodegradation, 2025)

Advancing bioremediation: biosurfactants as catalysts for sustainable remediation - Yashika Raheja; Poonam Sharma; Prachi Gaur; Vivek Kumar Gaur; Janmejai Kumar Srivastava (Biodegradation, 2025)

Sustainable bioremediation and reuse of heavy metal-contaminated dredged sediments using Bacillus subtilis - Kalyani Kulkarni; Nehal Jain; G. L. Sivakumar Babu (Biodegradation, 2025)

Ability of mixed fungal cultures to remove glyphosate from soil microcosms under stressful conditions - Melisa Eglé Aluffi; Karen Magnoli; Cecilia Soledad Carranza; Virginia Carolina Aparicio; Carla Lorena Barberis; Carina Elizabeth Magnoli (Biodegradation, 2025)

Inferences made from mineral elements characterisation of anaerobic digestion substrates: preliminary results from avocado oil processing byproducts - Charles Rashama; Grace N. Ijoma; Tonderayi S. Matambo; Christian Riann (Biodegradation, 2025)
The mineral element levels in avocado oil processing byproducts were evaluated to make inferences about these byproducts’ biodegradability behaviour. The mineral assays were determined using ICP-OES methods. Literature was also reviewed to understand how different mineral elements detected in substrates could impact biogas production under anaerobic digestion (AD) conditions. It was noted that there is no consensus among scholars on absolute mineral requirement levels for optimal digester performance since digesters use different substrates operating under varying conditions in most cases. When mineral elements are below certain values, the digester operates sub-optimally. When certain mineral levels are exceeded in digesters, the minerals become toxic to the AD microorganisms and destabilise the biogas digester. Only Zn levels across all avocado oil processing byproducts and K levels in cold-pressed decanter wastewater (CDW) exceeded the toxic level limits pegged at 1 mg/L and 3000 mg/L respectively. These may therefore need corrective action to avoid potential inhibition of the AD process. Mineral assays that seemed adequate and required no supplementation or detoxification were K, P, Fe, Cu, Se and Cr across the studied byproducts. All the other elements (excluding the Hg, As and Cd which are toxic) may require supplementation for optimal digester performances when using avocado oil processing byproducts as biogas digester substrates. However, these inferences may be affected by issues of mineral bioavailability which is a substrate-dependent phenomenon. Mercury cadmium and arsenic are naturally toxic to microorganisms and must be removed or detoxified if detected in substrates. The current study indicates the significance of mineral elements’ characterisation in explaining and potentially guiding the optimisation of substrate biodegradation to biogas.

Biodegradable polymer films incorporating phycocyanin and polyhydroxybutyrate: advancing sustainable packaging technologies - Alireza Farrokheh; Zahed Ahmadi (Biodegradation, 2025)

A comparative study using response surface methodology and artificial neural network for modeling the bio-reduction of hexavalent chromium (Cr⁶⁺) by immobilized cells of Paenibacillus taichungensis strain MAHA in an alginate-gellan gum matrix - Maha Obaid Al-Osaimi; Mohd Izuan Effendi Halmi; Siti Salwa Abd Gani; Khairil Mahmud; Mohd Yunus Abd Shukor (Biodegradation, 2025)

Challenges of exopolysaccharides production from polystyrene degradation by bacterium CHB 1.5 strain - Saijai Wattanasen; Pajongsuk Sutarut; Areeya Taengnoi; Salwa Torpee (Biodegradation, 2025)

A comprehensive review on microbial production and significant applications of multifunctional biomolecules: biosurfactants - Nisha Sharma; Yogesh K. Ahlawat; Arti Jamwal Sharma; Navneeti Chamoli; Monika Thakur; Anupriya Sharma; Sajid Mehmood; Anurag Malik; Meraj Ahmed; Himani Punia; Sumati Choubey (Biodegradation, 2025)

Enhanced bio-reduction of Cr(VI) using Shewanella putrefaciens CN32 mediated by Fe(III) minerals and riboflavin synergistically - Tianle Zhang; Haibo Li; Yichen Wu; Yajue Yuan; Yu Du (Biodegradation, 2025)

Advances in actinobacteria-based bioremediation: mechanistic insights, genetic regulation, and emerging technologies - Naureenbanu Makarani; Radhey Shyam Kaushal (Biodegradation, 2025)

Recent perspectives on biotechnological production, modulation and applications of glycerophosphoryl diester phosphodiesterases - Arshia Nazir; Muhammad Sajjad (Biodegradation, 2025)

Biochar mediated fixation of nitrogen compounds (ammonia and nitrite) in soil: a review - Arti Chamoli; Santosh Kumar Karn; Moni Kumari; Elayaraja Sivaramasamy (Biodegradation, 2025)

Migration of stable release high concentration toluene vapor and microbial activity varies in silty sand soil - Zhongping Sun; Yaju Gong; Wenxia Wei; Yun Song (Biodegradation, 2025)

Genome analysis of a newly isolated Lysinibacillus fusiformis–YC01 for biodegrading inosine and guanosine - Yu Zhang; Xiaoyu Cao; Jingyuan Cai; Meijie Song; Xinyue Du; Yang Liu; Qianqian Xu; Hai Yan (Biodegradation, 2025)

Combatting pesticide pollution: using liquid scintillation spectrometry to assess 14C-labeled hexachlorobenzene removal by mangrove Bacillus spp. - Wanessa J. Santana Mota; Jessica R. de Jesus; Ana P. Justiniano Rego; Ayslan S. P. da Costa; Valdemar L. Tornisielo; Katlin I. Barrios Eguiluz; Giancarlo R. Salazar-Banda; Maria L. Hernández-Macedo; Jorge A. López (Biodegradation, 2025)

Degradation of polyvinyl chloride (PVC) microplastics employing the actinobacterial strain Streptomyces gobitricini - Fuad Ameen; Hind A. Al-Shwaiman; Rania Almalki; Ahmed E. Al-Sabri; Essam N. Sholkamy (Biodegradation, 2025)

Investıgatıon of seam performance and bıodegradabılıty of organıc cotton clothes for theır sustaınabılıty - Hatice Kübra Özbey; Ayça Gürarda (Biodegradation, 2025)
In recent years, it has become important for a sustainable environment that clothes are biodegradable, and that unused clothing does not pollute the environment by decomposing in nature. Cotton, especially organic cotton, can decompose very quickly in nature. Since organic clothing production has become important in recent years, the seam performance and biodegradability of these products, unlike conventional products, are important in creating new data for the textile and clothing industry. This paper presents an experimental study of the seam performance and biodegradability properties of organic clothes for their sustainability. In this study, six groups of fabrics with a plain weave structure were produced to examine the seam performance and biodegradability of clothes made of organic cotton woven fabrics. Each group includes three different samples of the fabric: conventional cotton fabric dyed with reactive dyes, organic cotton fabric dyed with reactive dyes, and organic cotton fabric dyed with natural dye. Therefore 18 samples having different properties were obtained. Fabric breaking strength, seam strength, seam efficiency and biodegradability tests of these fabric samples were determined. Sample women's blouses were sewn from the fabric samples used in the experimental study, and the blouse appearances were visually presented on the mannequin.

Valorization of chicken feathers for polyhydroxyalkanoates production using Rohodococcus quinshengii LS18 and the biodegradation studies with the extracted polymer and its blends - Shareen Fatima Rizvi; Syed Khalida Izhar; Aamina Shahab; Akil Ahmad; Mohammed B. Alshammari; Mohd. Rehan Zaheer; Roohi Roohi (Biodegradation, 2025)

Isolation, characterization, and mycostimulation of fungi for the degradation of polycyclic aromatic hydrocarbons at a superfund site - Joshua Crittenden; Daniel Raudabaugh; Claudia K. Gunsch (Biodegradation, 2025)

Optimization of polyhydroxyalkanoate (PHA) production from biohythane pilot plant effluent by Cupriavidus necator TISTR 1335 - Yu-Ting Song; Napapat Sitthikitpanya; Nikannapas Usmanbaha; Alissara Reungsang; Chen-Yeon Chu (Biodegradation, 2025)

Advances in waste-derived functional materials for PFAS remediation - Saba Andleeb; Muhammad Irfan; Emmanuel Atta-Obeng; Dalia Sukmawati (Biodegradation, 2025)

Exploring the intricate studies on low-density polyethylene (LDPE) biodegradation by Bacillus cereus AP-01, isolated from the gut of Styrofoam-fed Tenebrio molitor larvae - Krishnamoorthi Akash; Rengasamy Parthasarathi; Rajavel Elango; Subramanian Bragadeeswaran (Biodegradation, 2025)

Predictive modeling of diazinon residual concentration in soils contaminated with potentially toxic elements: a comparative study of machine learning approaches - Marzieh Mohammadi Aria; Safar Vafadar; Yousef Sharafi; Abbas Ali Ghezelsofloo (Biodegradation, 2025)

Simultaneous devulcanization and denitrification: a novel approach for valorization of both ground tire rubber and nitrate-containing wastewater - Tercan Çataklı; Tuba Hande Erguder (Biodegradation, 2025)

Microbial diversity of dishwashers in different cities of China in spring, summer, autumn and winter - Shuai Yuan; Su Yao; Lingling Pang; Hua Xu; Feirong Bai; Jiali Kang (Biodegradation, 2025)

Evaluation of microbial community dynamics and chlorinated solvent biodegradation in methane-amended microcosms from an acidic aquifer - Myung Hwangbo; Rachael Rezes; Kung-Hui Chu; Paul B. Hatzinger (Biodegradation, 2025)

Disentangling the microbial genomic traits associated with aromatic hydrocarbon degradation in a jet fuel-contaminated aquifer - K. J. Hidalgo; V. B. Centurion; L. N. Lemos; A. U. Soriano; E. Valoni; M. P. Baessa; H. H. Richnow; C. Vogt; V. M. Oliveira (Biodegradation, 2025)

Revolutionizing dairy waste: emerging solutions in conjunction with microbial engineering - Rishi Bhatia; Shambhavi Singh; Vikram Kumar; Neetu K. Taneja; Harinder Singh Oberoi; Komal Chauhan (Biodegradation, 2025)

Removal mechanisms of pentachlorophenol in a horizontal-flow anaerobic immobilized biomass reactor (HAIB) inoculated with an indigenous estuarine sediment microbiota: adsorption and biodegradation processes - Gunther Brucha; Alessandra Giordani; Bárbara Franco Vieira; Marcia Helena Rissato Zamariolli Damianovic; Flavia Talarico Saia; Leonardo Henrique Soares Damasceno; Johannes Gerson Janzen; Eugenio Foresti; Rosana Filomena Vazoller (Biodegradation, 2025)

Isolation and purification of esterase enzyme from marine bacteria associated with biodegradation of polyvinyl chloride (PVC) - Shrikant D. Khandare; Doongar R. Chaudhary; Bhavanath Jha (Biodegradation, 2025)

Insights of energy potential in thermophilic sugarcane vinasse and molasses treatment: does two-stage codigestion enhance operational performance? - Alexandre Rodrigues Ribeiro; Kauanna Uyara Devens; Franciele Pereira Camargo; Isabel Kimiko Sakamoto; Maria Bernadete Amâncio Varesche; Edson Luiz Silva (Biodegradation, 2025)

Mutations of methionine 444 interacting with T1Cu-coordinating amino acids affect the structure and function of multicopper oxidase CopA - Wenwei Tang; Peiqi Zhang; Xiaoyu Jin; Xiaorong Li; Shichao Chen; Xinping Zeng (Biodegradation, 2025)

S0-dependent bio-reduction for antimonate detoxification from wastewater by an autotrophic bioreactor with internal recirculation - Dongjin Wan; Zhan Shen; Naiyuan Shi; Jiekai Wang; Weichao Zhang; Yahui Shi; Panting Wang; Qiaochong He (Biodegradation, 2025)

Correction: Enterobacter cloacae-mediated polymer biodegradation: in-silico analysis predicts broad spectrum degradation potential by Alkane monooxygenase - Mohamed Shafana Farveen; Rajnish Narayanan (Biodegradation, 2024)

Correction: Association of Laccase from Bacillus cereus O2-B and Pseudomonas aeruginosa O1-P with the bio-degradation of polymers: an in vitro to in silico approach - Mohamed Shafana Farveen; Thirumurthy Madhavan; Rajnish Narayanan (Biodegradation, 2024)

Developing a microbial community structure index (MCSI) as an approach to evaluate and optimize bioremediation performance - Jeff Gamlin; Renee Caird; Neha Sachdeva; Yu Miao; Claudia Walecka-Hutchison; Shaily Mahendra; Susan K. De Long (Biodegradation, 2024)

Delineating acetaminophen biodegradation kinetics and metabolomics using bacterial community - Bhavana Pandey; Suresh Kumar Dubey (Biodegradation, 2024)

Enterobacter cloacae-mediated polymer biodegradation: in-silico analysis predicts broad spectrum degradation potential by Alkane monooxygenase - Shafana Farveen Mohamed; Rajnish Narayanan (Biodegradation, 2024)

Biodegradable plastics: mechanisms of degradation and generated bio microplastic impact on soil health - Rishpreet Kaur; Indu Chauhan (Biodegradation, 2024)

Alternative green application areas for olive pomace catalytic pyrolysis biochar obtained via marble sludge catalyst - Gamze Goktepeli; Afra Ozgan; Vildan Onen; Gulnare Ahmetli; Merve Kalem; Esra Yel (Biodegradation, 2024)
Evaluating industrial wastes in the system with minimum preprocessing and generation economically valuable products from them have critical importance. In this regard, especially cheap, wieldy, and readily available catalysts have been researched to increase variety of useful products in pyrolysis systems, to reduce process time, and to increase quality and diversity of products. Therefore, in this study, marble sludge (named K1) was evaluated as catalyst at different dosages (10%, 20%, 30%, 50%) and pyrolysis temperatures (300, 500, 700 °C) in olive pomace (OP) pyrolysis and; the potential green applications of produced new biochars at new usage areas with different purposes based on characteristics were investigated. ANOVA test results showed that temperature and catalysts ratio had significant effect on pyrolysis product yields since significance value for K1 and temperature was lower than 0.05 for pyrolysis products. OP-K1 biochars had alkaline properties and high earth metal quantities. Moreover, increment in K1 ratio and temperature resulted in decrement of the biochar surface acidity. Therefore, it can be indicated that these biochars can have a potential usage for anaerobic digestion processes, lithium-ion batteries, and direct carbon solid oxide fuel cell (DC-SOFC) but further electrochemical property test should be performed. Moreover, produced biochars can be alternative fuels in some processes instead of coal since they have low S content and high heat values. Consequently, it is foreseen that produced biochars will have an important place in the development of potential usage areas with a new and environmentally friendly approach in different areas apart from the conventional uses of catalytic pyrolysis chars. Graphical abstract

Enhancing biodegradation of aged hydrocarbon-contaminated soils through toluene addition: assessing effects on solid and slurry phase treatments - M. E. Maya-Yescas; M. Gutiérrez-Rojas†; M. García-Rivero (Biodegradation, 2024)

Sustainable kitchen wastewater treatment with electricity generation using upflow biofilter-microbial fuel cell system - Ahmed Y. Radeef; Aya A. Najim; Haneen A. Karaghool; Zaid H. Jabbar (Biodegradation, 2024)

Three strategy rules of filamentous fungi in hydrocarbon remediation: an overview - Micheal Helan Soundra Rani; Ramesh Kumar Nandana; Alisha Khatun; Velumani Brindha; Durairaj Midhun; Ponnusamy Gowtham; Siva Shanmugam Dhanush Mani; Sivaraman Rathish Kumar; Anguraj Aswini; Sugumar Muthukumar (Biodegradation, 2024)

Study on microbial diversity of washing machines - Yunyi Cao; Shuai Yuan; Lingling Pang; Jiuyan Xie; Yi Gao; Jian Zhang; Zhenyao Zhao; Su Yao (Biodegradation, 2024)

A novel coupling process to replace the traditional multi-stage anammox process—sulfur autotrophic denitrification coupled anammox system - Sai Yao; Kuo Zhang; Song Yang; Zijun Li; Youzhao Wang; Feng Ma; Pu Chen; Tong Zhu (Biodegradation, 2024)

Anthracene detoxification by Laccases from indigenous fungal strains Trichoderma lixii FLU1 and Talaromyces pinophilus FLU12 - Samson O. Egbewale; Ajit Kumar; Tosin A. Olasehinde; Mduduzi P. Mokoena; Ademola O. Olaniran (Biodegradation, 2024)
The persistence and ubiquity of polycyclic aromatic hydrocarbons (PAHs) in the environment necessitate effective remediation strategies. Hence, this study investigated the potential of purified Laccases, Tl FLU1L and Tp FLU12L, from two indigenous fungi Trichoderma lixii FLU1 ( Tl FLU1) and Talaromyces pinophilus FLU12 ( Tp FLU12), respectively for the oxidation and detoxification of anthracene. Anthracene was degraded with v max values of 3.51 ± 0.06 mg/L/h and 3.44 ± 0.06 mg/L/h, and K m values of 173.2 ± 0.06 mg/L and 73.3 ± 0.07 mg/L by Tl FLU1L and Tp FLU12L, respectively. The addition of a mediator compound 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) to the reaction system significantly increased the degradation of anthracene, with up to a 2.9-fold increase in v max value and up to threefold decrease in K m values of Tl FLU1L and Tp FLU12L. The GC–MS analysis of the metabolites suggests that anthracene degradation follows one new pathway unique to the ABTS system—hydroxylation and carboxylation of C-1 and C-2 position of anthracene to form 3-hydroxy-2-naphthoic acid, before undergoing dioxygenation and side chain removal to form chromone which was later converted into benzoic acid and CO 2 . This pathway contrasts with the common dioxygenation route observed in the free Laccase system, which is observed in the second degradation pathways. Furthermore, toxicity tests using V. parahaemolyticus and HT-22 cells, respectively, demonstrated the non-toxic nature of Laccase-ABTS-mediated metabolites. Intriguingly, analysis of the expression level of Alzheimer’s related genes in HT-22 cells exposed to degradation products revealed no induction of neurotoxicity unlike untreated cells. These findings propose a paradigm shift for bioremediation by highlighting the Laccase-ABTS system as a promising green technology due to its efficiency with the discovery of a potentially less harmful degradation pathway, and the production of non-toxic metabolites.

Exploring the potential of biofiltration for mitigating harmful gaseous emissions from small or old landfills: a review - El Farouk Omar Merouani; Milad Ferdowsi; Gerardo Buelna; J. Peter Jones; El-Hadi Benyoussef; Luc Malhautier; Michèle Heitz (Biodegradation, 2024)

Inclusion of Pseudomonas fluorescens into soil-like fraction from municipal solid waste management park to enhance plastic biodegradation - S. Sangeetha; P. Hari Krishna (Biodegradation, 2024)

Bioremediation of petroleum refinery wastewater by fungal stains isolated from the fishing harbour of Bizerte (Mediterranean Sea) - Tahani El Ayari; Rihab Bouhdida; Hadda Imene Ouzari; Najoua Trigui El Menif (Biodegradation, 2024)

Valorization of waste engine oil to mono- and di-rhamnolipid in a sustainable approach to circular bioeconomy - Shailee Gaur; Mohan Jujaru; Revanth Vennu; Suresh Gupta; Amit Jain (Biodegradation, 2024)

Degradation of pretilachlor and fenclorim and effects of these compounds on bacterial communities under anaerobic condition - Ha Danh Duc; Nguyen Thi Oanh; Nguyen Thi Dieu Thuy; Nguyen Thi Kim Xuan (Biodegradation, 2024)

Bioprospecting of extremophilic perchlorate-reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia - Rosa Acevedo-Barrios; Irina Tirado-Ballestas; Angela Bertel-Sevilla; Leonor Cervantes-Ceballos; Jorge L. Gallego; María Angélica Leal; David Tovar; Jesús Olivero-Verbel (Biodegradation, 2024)
Three extremophile bacterial strains (BBCOL-009, BBCOL-014 and BBCOL-015), capable of degrading high concentrations of perchlorate at a range of pH (6.5 to 10.0), were isolated from Colombian Caribbean Coast sediments. Morphological features included Gram negative strain bacilli with sizes averaged of 1.75 × 0.95, 2.32 × 0.65 and 3.08 × 0.70 μm, respectively. The reported strains tolerate a wide range of pH (6.5 to 10.0); concentrations of NaCl (3.5 to 7.5% w/v) and KClO 4 − (250 to 10000 mg/L), reduction of KClO 4 − from 10 to 25%. LB broth with NaCl (3.5–30% w/v) and KClO4ˉ (250-10000 mg/L) were used in independent trials to evaluate susceptibility to salinity and perchlorate, respectively. Isolates increased their biomass at 7.5 % (w/v) NaCl with optimal development at 3.5 % NaCl. Subsequently, ClO 4 ˉ reduction was assessed using LB medium with 3.5% NaCl and 10000 mg/L ClO 4 ˉ. BBCOL-009, BBCOL-014 and BBCOL-015 achieved 10%, 17%, and 25% reduction of ClO 4 ˉ, respectively. The 16 S rRNA gene sequence grouped them as Bacillus flexus T6186-2, Bacillus marisflavi TF-11 (T), and Bacillus vietnamensis 15 − 1 (T) respectively, with

Performance, kinetic characteristics and bacterial community of short-cut nitrification and denitrification system at different ferrous ion conditions - Ben-ze Chang; Shuai Zhang; Dong-zhi Chen; Kai-tuo Gao; Guang-feng Yang (Biodegradation, 2024)

Genome analysis of a newly isolated Bacillus velezensis-YW01 for biodegrading acetaldehyde - Jingjing Wang; Zhihao Wang; Chao Liu; Meijie Song; Qianqian Xu; Yang Liu; Hai Yan (Biodegradation, 2024)

Phenol biodegradation using bio-filter tower packed column with immobilized bacterial consortium: a batch test study - Preeti Sachan; Athar Hussain; Sangeeta Madan; Utkarsh Singh; Manjeeta Priyadarshi (Biodegradation, 2024)

Isolation, identification and transcriptome analysis of triadimefon-degrading strain Enterobacter hormaechei TY18 - Yan Wang; Qi Guan; Wenhui Jiao; Jiangbo Li; Rui Zhao; Xiqian Zhang; Weixin Fan; Chunwei Wang (Biodegradation, 2024)

Modeling key intermediates during anaerobic digestion of lipid rich kitchen waste with an extended ADM1 - Simon Weber (Biodegradation, 2024)

Biodegradation of a complex hydrocarbon mixture and biosurfactant production by Burkholderia thailandensis E264 and an adapted microbial consortium - Emmeline D’Incau; Stéphanie Ouvrard; Marion Devers-Lamrani; Carole Jeandel; Chems Eddine Mohamed; Sonia Henry (Biodegradation, 2024)

Biodegradation of polyethylene terephthalate (PET) by Brucella intermedia IITR130 and its proposed metabolic pathway - Pallavi Srivastava; Joel Saji; Natesan Manickam (Biodegradation, 2024)

Enhancing bioremediation potential of microalgae Chlorella vulgaris and Scenedesmus acutus by NaCl for pyrene degradation - Rupal Singh Tomar; Prabha Rai-Kalal; Anjana Jajoo (Biodegradation, 2024)

Exploring polyhydroxyalkanoates biosynthesis using hydrocarbons as carbon source: a comprehensive review - G. Corti Monzón; G. Bertola; M. K. Herrera Seitz; S. E. Murialdo (Biodegradation, 2024)

Eco-friendly approaches for mitigating plastic pollution: advancements and implications for a greener future - Ayesha Safdar; Fatima Ismail; Maryem Safdar; Muhammad Imran (Biodegradation, 2024)

Isolation and characterization of distinctive pyrene-degrading bacteria from an uncontaminated soil - Shanshan Sun; Ran Wei; Siyi Hu; Meiyu Yang; Jinzhi Ni (Biodegradation, 2024)

Biodegradation of low-density polythene (LDPE) by a novel strain of Pseudomonas aeruginosa WD4 isolated from plastic dumpsite - Shilpa; Nitai Basak; Sumer Singh Meena (Biodegradation, 2024)

Batch-mode degradation of high-strength phenolic pollutants by Pseudomonas aeruginosa strain STV1713 immobilized on single and hybrid matrices - Reshmi Sasi; Suchithra Tharamel Vasu (Biodegradation, 2024)

Biodegradation of PVCs through in-vitro identification of Bacillus albus and computational pathway analysis of ABH enzyme - Muhammad Naveed; Rida Naveed; Tariq Aziz; Arooj Azeem; Mahrukh Afzal; Muhammad Waseem; Metab Alharbi; Abdulrahman Alshammari; Abdullah F. Alasmari; Thamer H. Albekairi (Biodegradation, 2024)

Mechanically stirred enzymatic membrane reactor containing HRP immobilized on Tau-SiO2@Fe3O4-GO nanocomposite for removal of tetracycline in synthetically concocted wastewater - Monireh Besharati Vineh; Amir Ali Poostchi; Davood Kordestani; Mahmoud Karami Qushehbolagh; Ali Akbar Saboury (Biodegradation, 2024)

Mitigation of Desulfovibrio ferrophilus IS5 degradation of X80 carbon steel mechanical properties using a green biocide - Zhong Li; Jike Yang; Shihang Lu; Wenwen Dou; Tingyue Gu (Biodegradation, 2024)

Identification of bacterial community in a rapid composting method using 16SrDNA genes sequencing - Muhammad Shahid; Waqar Ahmed; Abdul Basit Khan; Zafar Iqbal Shams; Afsheen Arif (Biodegradation, 2024)

Organic stabilization and methane production under different organic loading rates in UASB treating swine wastewater - Jacineumo Falcão de Oliveira; Ronaldo Fia; Ana Flavia Santos Rabelo de Melo; Fátima Resende Luiz Fia; Fernando Neris Rodrigues; Luciene Alves Batista Siniscalchi; Mateus Pimentel de Matos (Biodegradation, 2024)

Simultaneous biodegradation kinetics of 1,4-dioxane and ethane - Ermias Gebrekrstos Tesfamariam; Yi-Hao Luo; Chen Zhou; Ming Ye; Rosa Krajmalnik-Brown; Bruce E. Rittmann; Youneng Tang (Biodegradation, 2024)

Review of nomenclature and methods of analysis of polyethylene terephthalic acid hydrolyzing enzymes activity - Ruth Amanna; Sudip K. Rakshit (Biodegradation, 2024)

Bio-based polyester-polyurethane foams: synthesis and degradability by Aspergillus niger and Aspergillus clavatus - Mara L. Polo; Karen Russell-White; Santiago E. Vaillard; Luis Ríos; Gregorio R. Meira; Diana A. Estenoz; Marisa E. Spontón (Biodegradation, 2024)

Differences among active toluene-degrading microbial communities in farmland soils with different levels of heavy metal pollution - Fei Dou; Yundang Wu; Jibing Li; Chuanping Liu (Biodegradation, 2024)

Degradation of multiple PAHs and co-contaminants by microbial consortia and their toxicity assessment - Arfin Imam; Sunil Kumar Suman; Sonpal Vasavdutta; Shruti Chatterjee; Bhanu Prasad Vempatapu; Anjan Ray; Pankaj K. Kanaujia (Biodegradation, 2024)

Landfill leachate treatment using fungi and fungal enzymes: a review - Anusree Nalladiyil; P. Sughosh; G. L. Sivakumar Babu; Sreenivasan Ramaswami (Biodegradation, 2024)

Myco-remediation of plastic pollution: current knowledge and future prospects - Somanjana Khatua; Jesus Simal-Gandara; Krishnendu Acharya (Biodegradation, 2024)
To date, enumerable fungi have been reported to participate in the biodegradation of several notorious plastic materials following their isolation from soil of plastic-dumping sites, marine water, waste of mulch films, landfills, plant parts and gut of wax moth. The general mechanism begins with formation of hydrophobin and biofilm proceding to secretion of specific plastic degarding enzymes (peroxidase, hydrolase, protease and urease), penetration of three dimensional substrates and mineralization of plastic polymers into harmless products. As a result, several synthetic polymers including polyethylene, polystyrene, polypropylene, polyvinyl chloride, polyurethane and/or bio-degradable plastics have been validated to deteriorate within months through the action of a wide variety of fungal strains predominantly Ascomycota ( Alternaria , Aspergillus , Cladosporium , Fusarium , Penicillium spp.). Understanding the potential and mode of operation of these organisms is thus of prime importance inspiring us to furnish an up to date view on all the presently known fungal strains claimed to mitigate the plastic waste problem. Future research henceforth needs to be directed towards metagenomic approach to distinguish polymer degrading microbial diversity followed by bio-augmentation to build fascinating future of waste disposal.

Bioaugmentation has temporary effect on anaerobic pesticide biodegradation in simulated groundwater systems - Andrea Aldas-Vargas; Jannigje G. Kers; Hauke Smidt; Huub H. M. Rijnaarts; Nora B. Sutton (Biodegradation, 2024)
Groundwater is the most important source for drinking water in The Netherlands. Groundwater quality is threatened by the presence of pesticides, and biodegradation is a natural process that can contribute to pesticide removal. Groundwater conditions are oligotrophic and thus biodegradation can be limited by the presence and development of microbial communities capable of biodegrading pesticides. For that reason, bioremediation technologies such as bioaugmentation (BA) can help to enhance pesticide biodegradation. We studied the effect of BA using enriched mixed inocula in two column bioreactors that simulate groundwater systems at naturally occurring redox conditions (iron and sulfate-reducing conditions). Columns were operated for around 800 days, and two BA inoculations (BA1 and BA2) were conducted in each column. Inocula were enriched from different wastewater treatment plants (WWTPs) under different redox-conditions. We observed a temporary effect of BA1, reaching 100% removal efficiency of the pesticide 2,4-D after 100 days in both columns. In the iron-reducing column, 2,4-D removal was in general higher than under sulfate-reducing conditions demonstrating the influence of redox conditions on overall biodegradation. We observed a temporary shift in microbial communities after BA1 that is relatable to the increase in 2,4-D removal efficiency. After BA2 under sulfate-reducing conditions, 2,4-D removal efficiency decreased, but no change in the column microbial communities was observed. The present study demonstrates that BA with a mixed inoculum can be a valuable technique for improving biodegradation in anoxic groundwater systems at different redox-conditions. Graphical abstract

Bioaugmentation: an approach to biological treatment of pollutants - Dixita Chettri; Ashwani Kumar Verma; Anil Kumar Verma (Biodegradation, 2024)

Biodegradation of commercial textile reactive dye mixtures by industrial effluent adapted bacterial consortium VITPBC6: a potential technique for treating textile effluents - Purbasha Saha; Kokati Venkata Bhaskara Rao (Biodegradation, 2024)

Purification and characterization of extracellular PHB depolymerase enzyme from Aeromonas caviae Kuk1-(34) and their biodegradation studies with polymer films - Mohd. Amir; Naushin Bano; Anamika Gupta; Mohd. Rehan Zaheer; Roohi (Biodegradation, 2024)

Biooxidation of hydrogen sulfide to sulfur by moderate thermophilic acidophilic bacteria - R. Romero; P. Viedma; D. Cotoras (Biodegradation, 2024)

Influence of microbial biomass content on biodegradation and mechanical properties of poly(3-hydroxybutyrate) composites - Felix Eckel; Korbinian Sinzinger; Daniel Van Opdenbosch; Doris Schieder; Volker Sieber; Cordt Zollfrank (Biodegradation, 2024)
Biodegradation rates and mechanical properties of poly(3-hydroxybutyrate) (PHB) composites with green algae and cyanobacteria were investigated for the first time. To the authors knowledge, the addition of microbial biomass led to the biggest observed effect on biodegradation so far. The composites with microbial biomass showed an acceleration of the biodegradation rate and a higher cumulative biodegradation within 132 days compared to PHB or the biomass alone. In order to determine the causes for the faster biodegradation, the molecular weight, the crystallinity, the water uptake, the microbial biomass composition and scanning electron microscope images were assessed. The molecular weight of the PHB in the composites was lower than that of pure PHB while the crystallinity and microbial biomass composition were the same for all samples. A direct correlation of water uptake and crystallinity with biodegradation rate could not be observed. While the degradation of molecular weight of PHB during sample preparation contributed to the improvement of biodegradation, the main reason was attributed to biostimulation by the added biomass. The resulting enhancement of the biodegradation rate appears to be unique in the field of polymer biodegradation. The tensile strength was lowered, elongation at break remained constant and Young’s modulus was increased compared to pure PHB.

Oxidation of chlortetracycline and its isomers by Botrytis aclada laccase in the absence of mediators: pH dependence and identification of transformation products by LC–MS - Nadia Gavilán de Fátima; Andrés Barriga; Juan Carlos Cáceres; Ernani Pinto; Ricardo Cabrera (Biodegradation, 2024)

Correction: Investigating the biological degradation of the drug β-blocker atenolol from wastewater using the SBR - Reza Rezaei; Ali Ahmad Aghapour; Hassan Khorsandi (Biodegradation, 2024)

Autochthonous psychrophilic hydrocarbonoclastic bacteria and its ecological function in contaminated cold environments - Pranjal Bharali; Bhagyudoy Gogoi; Viphrezolie Sorhie; Shiva Aley Acharjee; Bendangtula Walling; Alemtoshi; Vinita Vishwakarma; Maulin Pramod Shah (Biodegradation, 2024)

Anammox with alternative electron acceptors: perspectives for nitrogen removal from wastewaters - Sergio J. Ponce-Jahen; Bibiana Cercado; Edson Baltazar Estrada-Arriaga; J. Rene Rangel-Mendez; Francisco J. Cervantes (Biodegradation, 2024)
In the context of the anaerobic ammonium oxidation process (anammox), great scientific advances have been made over the past two decades, making anammox a consolidated technology widely used worldwide for nitrogen removal from wastewaters. This review provides a detailed and comprehensive description of the anammox process, the microorganisms involved and their metabolism. In addition, recent research on the application of the anammox process with alternative electron acceptors is described, highlighting the biochemical reactions involved, its advantages and potential applications for specific wastewaters. An updated description is also given of studies reporting the ability of microorganisms to couple the anammox process to extracellular electron transfer to insoluble electron acceptors; particularly iron, carbon-based materials and electrodes in bioelectrochemical systems (BES). The latter, also referred to as anodic anammox, is a promising strategy to combine the ammonium removal from wastewater with bioelectricity production, which is discussed here in terms of its efficiency, economic feasibility, and energetic aspects. Therefore, the information provided in this review is relevant for future applications.

Complete degradation of di-n-butyl phthalate by Glutamicibacter sp. strain 0426 with a novel pathway - Chongyang Ren; Yiying Wang; Yanan Wu; He-Ping Zhao; Li Li (Biodegradation, 2024)

Effect of copper, arsenic and nickel on pyrite-based autotrophic denitrification - Maria F. Carboni; Sonia Arriaga; Piet N. L. Lens (Biodegradation, 2024)
Pyritic minerals generally occur in nature together with other trace metals as impurities, that can be released during the ore oxidation. To investigate the role of such impurities, the presence of copper (Cu(II)), arsenic (As(III)) and nickel (Ni(II)) during pyrite mediated autotrophic denitrification has been explored in this study at 30 °C with a specialized microbial community of denitrifiers as inoculum. The three metal(loid)s were supplemented at an initial concentration of 2, 5, and 7.5 ppm and only Cu(II) had an inhibitory effect on the autotrophic denitrification. The presence of As(III) and Ni(II) enhanced the nitrate removal efficiency with autotrophic denitrification rates between 3.3 [7.5 ppm As(III)] and 1.6 [7.5 ppm Ni(II)] times faster than the experiment without any metal(loid) supplementation. The Cu(II) batches, instead, decreased the denitrification kinetics with 16, 40 and 28% compared to the no-metal(loid) control for the 2, 5 and 7.5 ppm incubations, respectively. The kinetic study revealed that autotrophic denitrification with pyrite as electron donor, also with Cu(II) and Ni(II) additions, fits better a zero-order model, while the As(III) incubation followed first-order kinetic. The investigation of the extracellular polymeric substances content and composition showed more abundance of proteins, fulvic and humic acids in the metal(loid) exposed biomass. Graphical Abstract

Effect of ultra-violet light radiation on Scenedesmus vacuolatus growth kinetics, metabolic performance, and preliminary biodegradation study - Stella B. Eregie; Isaac A. Sanusi; Gueguim E. B. Kana; Ademola O. Olaniran (Biodegradation, 2024)
This study presents the effect of ultra-violet (UV) light radiation on the process kinetics, metabolic performance, and biodegradation capability of Scenedesmus vacuolatus . The impact of the UV radiation on S. vacuolatus morphology, chlorophyll, carotenoid, carbohydrates, proteins, lipid accumulation, growth rate, substrate affinity and substrate versatility were evaluated. Thereafter, a preliminary biodegradative potential of UV-exposed S. vacuolatus on spent coolant waste (SCW) was carried out based on dehydrogenase activity (DHA) and total petroleum hydrocarbon degradation (TPH). Pronounced structural changes were observed in S. vacuolatus exposed to UV radiation for 24 h compared to the 2, 4, 6, 12 and 48 h UV exposure. Exposure of S. vacuolatus to UV radiation improved cellular chlorophyll (chla = 1.89-fold, chlb = 2.02-fold), carotenoid (1.24-fold), carbohydrates (4.62-fold), proteins (1.44-fold) and lipid accumulations (1.40-fold). In addition, the 24 h UV exposed S. vacuolatus showed a significant increase in substrate affinity (1/Ks) (0.959), specific growth rate (µ) (0.024 h −1 ) and biomass accumulation (0.513 g/L) by 1.50, 2 and 1.9-fold respectively. Moreover, enhanced DHA (55%) and TPH (100%) degradation efficiency were observed in UV-exposed S. vacuolatus . These findings provided major insights into the use of UV radiation to enhance S. vacuolatus biodegradative performance towards sustainable green environment negating the use of expensive chemicals and other unfriendly environmental practices. Graphical abstract

Biological control of some wood-decay fungi with antagonistic fungi - Selim Hınçal; Mesut Yalçın (Biodegradation, 2023)

Acidovorax PSJ13, a novel, efficient polyacrylamide-degrading bacterium by cleaving the main carbon chain skeleton without the production of acrylamide - Zhengjiang Wang; Kaili Li; Xuwei Gui; Zhenlun Li (Biodegradation, 2023)

Microorganisms that produce enzymes active on biodegradable polyesters are ubiquitous - Francesco Degli-Innocenti; Tony Breton; Selene Chinaglia; Ermes Esposito; Marco Pecchiari; Andrea Pennacchio; Alessandro Pischedda; Maurizio Tosin (Biodegradation, 2023)

Biodegradation of di-2-ethylhexyl phthalate by Bacillus firmus MP04 strain: parametric optimization using full factorial design - Madhavi Rashmi; Tanuja Singh; Nitesh Singh Rajput; Shweta Kulshreshtha (Biodegradation, 2023)

An indigenous tubular ceramic membrane integrated bioreactor system for biodegradation of phthalates mixture from contaminated wastewater - Dipak Kumar Kanaujiya; Madu Purnima; G. Pugazhenthi; Tapan Kumar Dutta; Kannan Pakshirajan (Biodegradation, 2023)

Potential of Antarctic lipase from Acinetobacter johnsonii Ant12 for treatment of lipid-rich wastewater: screening, production, properties and applications - Vijay D. Nimkande; Kannan Krishnamurthi; Amit Bafana (Biodegradation, 2023)

Cometabolic degradation of pyrene with phenanthrene as substrate: assisted by halophilic Pseudomonas stutzeri DJP1 - Junfeng Jiang; Weijun Tian; Zhiyang Lu; Meile Chu; Huimin Cao; Dantong Zhang (Biodegradation, 2023)

Effects of landfill food waste diversion: a focus on microbial populations and methane generation - Giles Chickering; Max J. Krause; Amy Schwarber (Biodegradation, 2023)

Influence of growth substrate and contaminant mixtures on the degradation of BTEX and MTBE by Rhodococcus rhodochrous ATCC strain 21198 - Juliana M. Huizenga; Lewis Semprini (Biodegradation, 2023)

Microalgal conversion of whey and lactose containing substrates: current state and challenges - Sergejs Kolesovs; Pavels Semjonovs (Biodegradation, 2023)

Isolation of cadmium-resistant microbial strains and their immobilisation of cadmium in soil - Ruijuan Fan; Weixia Xie; Heqin Ma; Mengke Zhu; Kun Ma; Xingfu Yan (Biodegradation, 2023)

Plasticizer phthalate esters degradation with a laccase from Trametes versicolor: effects of TEMPO used as a mediator and estrogenic activity removal - Dan Wang; Ruofei Zhu; Jiangfei Lou; Nawon Baek; Xuerong Fan (Biodegradation, 2023)

A sustainable approach to enhance heavy hydrocarbons removal in landfarming treatment - Camilla Di Marcantonio; Agostina Chiavola; Alessandra Noce; Elisabetta Straccamore; Andrea Giannuzzi; Jacopo Jirillo; Francesco Gallo; Maria Rosaria Boni (Biodegradation, 2023)
The present study aimed to evaluate the best strategy to enhance the degradation rate of heavy petroleum hydrocarbons (HPH) contaminated soil in a landfarming plant. Samples of real contaminated soil, further spiked with HPH, were treated in mesocosm reactors simulating the landfarming system. One reactor was operated without any modification compared to the real landfarming plant. The other three reactors were operated with different strategies to improve the removal rate: biostimulation (BS) through the addition of nitrogen and phosphorus; bioaugmentation (BA) with the inoculation of sludge produced in the treatment of the process water from the oil re-fining plant of the same industrial area; combination of biostimulation and bioaugmentation (BAS). The biostimulation (BS) was the most effective strategy, leading to a reduction of the remediation time by 35% as compared to the traditional treatment. Bioaugmentation (BA) also provided positive effects leading to a reduction of the remediation time by 24%; its performance improved further when the addition of sludge was combined with the increase of phosphorous (BAS). Therefore, the key tool was represented by the phosphorous availability, whereas the application of sludge was most useful to provide waste with a new possibility of reuse, thus fulfilling the principles of the circular economy. The final characterization showed that the treated soil was suitable for reuse in industrial areas according to the legislation in force.

Association of Laccase from Bacillus cereus O2-B and Pseudomonas aeruginosa O1-P with the bio-degradation of polymers: an in vitro to in silico approach - Mohamed Shafana Farveen; Thirumurthy Madhavan; Rajnish Narayanan (Biodegradation, 2023)

Identification of two possible metabolic pathways responsible for the biodegradation of 3, 5, 6-trichloro-2-pyridinol in Micrococcus luteus ML - Caixu Yue; Nan Jia; Xueru Lv; Shenghui Wang (Biodegradation, 2023)

Lead or cadmium co-contamination alters benzene and toluene degrading bacterial communities - Aniko Konya; Brice A. Fiddler; Olivia Bunch; Kendra Z. Hess; Cade Ferguson; Mark J. Krzmarzick (Biodegradation, 2023)
Co-contamination of hydrocarbons with heavy metals in soils often complicates and hinders bioremediation. A comprehensive characterization of site-specific degraders at contaminated sites can help determine if in situ bioremediation processes are sufficient. This study aimed to identify differences in benzene and toluene degradation rates and the microbial communities enriched under aerobic conditions when different concentrations of Cd and Pb are introduced. Microcosms were used to study the degradation of 0.23 mM benzene or 0.19 mM toluene under various concentrations of Pb (up to 240 µM) and Cd (up to 440 µM). Soil collected from a stormwater retention basin receiving runoff from a large parking lot was utilized to seed the microcosms. The hydrocarbon degradation time and rates were measured. After further rounds of amendment and degradation of benzene and toluene, 16S rRNA gene amplicon sequencing and quantitative PCR were used to ascertain the microbial communities enriched under the various concentrations of the heavy metals. The initial degradation time for toluene and benzene was 7 to 9 days and 10 to 13 days, respectively. Degradation rates were similar for each hydrocarbon despite the concentration and presence of metal co-contaminant, however, the enriched microbial communities under each condition differed. Microcosms without metal co-contaminant contained a diversity of putative benzene and toluene degrading bacteria. Cd strongly reduced the richness of the microbial communities. With higher levels of heavy metals, genera such as Ralstonia , Cupriavidus , Azoarcus , and Rhodococcus became more dominant under various conditions. The study finds that highly efficient benzene- and toluene-degrading consortia can develop under variations of heavy metal co-contamination, but the consortia are dependent on the heavy metal type and concentrations.

Biological degradation and mineralization of tetracycline antibiotic using SBR equipped with a vertical axially rotating biological bed (SBR-VARB) - Ali Ahmad Aghapour; Nazila Alizadeh; Hassan Khorsandi (Biodegradation, 2023)
Tetracycline (TC) is a widely used antibiotic with a complex aromatic chemical structure and is highly resistant to biodegradation. In this study, an SBR equipped with a vertical axially rotating biological bed (SBR-VARB) was used for the biodegradation and mineralization of TC. SBR-VARB showed high efficiency in removing TC (97%), total phenolic compounds (TP) (95%), and COD (85%) under optimal operating conditions (TC = 50 mg/L, HRT = 1.75 d, and OLR = 36 g COD/m 3 d). The SBR-VARB was able to treat higher concentrations of TC in shorter HRT than reported in previous studies. The contribution of VARB to improve SBR efficiency in removing TC, TP, and COD was 16, 36, and 48%, respectively. Intermediate compounds formed during the biodegradation of TC were identified using GC–MS under the optimal operating conditions of the bioreactor. These are mainly organic compounds with linear chemical structures. Based on the complete biodegradation of TC under the optimal operating conditions of the bioreactor, 93% and 36% of the chlorine and nitrogen atoms in the chemical structure of TC appeared in the wastewater, respectively. According to the sequence analysis of 16SrDNA, Pseudomonas sp., Kocuria Polaris , and Staphylococcus sp. were identified in the biofilm of VARB and the suspended biomass of the bioreactor. Therefore, SBR-VARB showed high efficiency in the biodegradation and mineralization of TC and can be used as a suitable option for treating wastewater containing antibiotics and other toxic compounds.

Biodegradation of sulfoxaflor and photolysis of sulfoxaflor by ultraviolet radiation - Yun-Xiu Zhao; Ke-Xin Chen; Li Wang; Pan-Pan Yuan; Yi-Jun Dai (Biodegradation, 2023)

Impacts of co-contaminants and dilution on perchlorate biodegradation using various carbon sources - Yasaman Saedi; Jacimaria R. Batista; Ronnie Britto; Dana Grady (Biodegradation, 2023)

Enhancement of biogranules development using magnetized powder activated carbon - Ahmad Hanis Omar; Khalida Muda; Armstrong Ighodalo Omoregie; Zaiton Abdul Majid; Nur Shahidah Binti Aftar Ali; Farhan Mohd Pauzi (Biodegradation, 2023)

Fungal biodegradation of chlorinated herbicides: an overview with an emphasis on 2,4-D in Argentina - Karen Magnoli; Cecilia Carranza; Melisa Aluffi; Carina Magnoli; Carla Barberis (Biodegradation, 2023)
Chlorinated herbicides are one of the main types of pesticide used in agriculture. In Argentina, 2,4-dichlorophenoxyacetic acid (2,4-D) is the most applied herbicide for the control of broadleaf weeds, but the risks it poses for the environment and human health are cause for great concern. A promising technology to remove this kind of pollutants, or neutralize them in such a way that they become less or non-toxic, is the use of degrading or detoxifying microorganisms from contaminated sites. Filamentous fungi can bioremediate xenobiotics thanks to their efficient enzymatic machinery. However, most studies on the degradation of 2,4-D have been carried out with bacteria, and little is known about whether it can be efficiently biodegraded by fungi. In the environment, fungal strains and native microbiota may detoxify contaminants through mechanisms like biosorption, bioabsortion, biotransformation, and/or degradation. Whether these processes occur separately or simultaneously depends on the metabolic ability of the strains that conform the microbial community. Another important concern when attempting to introduce detoxifying microorganisms into a contaminated environment is the GRAS (“Generally Recognized As Safe”) assessment or status. These are studies that help predict a biodegrading microorganism’s pathogenicity, toxicity , and infectivity before in situ application. This application, moreover, is regulated by different legal frameworks. The present review aims to outline the main aspects of 2,4-D degradation by fungi, and to summarize the current state of research on the topic in Argentina.

Tramates trogii biomass in carboxymethylcellulose-lignin composite beads for adsorption and biodegradation of bisphenol A - Gulay Bayramoglu; Murat Kilic; Mehmet Yakup Arica (Biodegradation, 2023)

Biodegradation of 1,4-dioxane by a native digestate microbial community under different electron accepting conditions - Aryan Samadi; Azadeh Kermanshahi-pour; Suzanne M. Budge; Yannan Huang; Rob Jamieson (Biodegradation, 2023)

Nondesulfurizing benzothiophene biotransformation to hetero and homodimeric ortho-substituted diaryl disulfides by the model PAH-degrading Sphingobium barthaii - Yuki Nemoto; Kohei Ozawa; Jiro F. Mori; Robert A. Kanaly (Biodegradation, 2023)

Evaluating the suitability of granular anammox biomass for nitrogen removal from vegetable tannery wastewater - C. Polizzi; T. Lotti; A. Ricoveri; G. Mori; D. Gabriel; G. Munz (Biodegradation, 2023)
In the present study, the potential inhibitory effect of biologically pre-treated vegetable tannery wastewater (TW) on anammox granular biomass was evaluated. Beside high organic and chemicals load, vegetable TW are characterised by high salinity and high tannins concentration, the latter belonging to a group of bio-refractory organic compounds, potentially inhibitory for several bacterial species. Recalcitrant tannin-related organic matters and salinity were selected as the two potential inhibitory factors and studied either for their separate and combined effect. Parallel batch tests were performed, with biomass acclimated and non-acclimated to salinity, testing three different conditions: non-saline control test with non-acclimated biomass (CT); saline control test with acclimated biomass (SCT); vegetable tannery wastewater test with acclimated biomass (TWT). Compared with non-saline CT, the specific anammox activity in tests SCT and TWT showed a reduction of 28 and 14%, respectively, suggesting that salinity, at conductivity values of 10 mS/cm (at 25 °C), was the main impacting parameter. As a general conclusion, the study reveals that there is no technical limitation for the application of the anammox process to vegetable TW, but preliminary biomass acclimation as well as regular biomass activity monitoring is recommended in case of long-term applications. To the best of our knowledge, this is the first work assessing the impact of vegetable TW on anammox biomass.

Recent advances in simultaneous nitrification and denitrification for nitrogen and micropollutant removal: a review - Susan N. James; Arya Vijayanandan (Biodegradation, 2023)

Isolation and identification of the molybdenum-resistant strain Raoultella ornithinolytica A1 and its effect on MoO42− in the environment - Jie Xing; Chunyan Li; Wanting Li; Xuemei Zhang; Zhaoquan Li; Ang Li (Biodegradation, 2023)

Metaproteomic and gene expression analysis of interspecies interactions in a PAH-degrading synthetic microbial consortium constructed with the key microbes of a natural consortium - Esteban E. Nieto; Marianela Macchi; María P. Valacco; Sabrina Festa; Irma S. Morelli; Bibiana M. Coppotelli (Biodegradation, 2023)

Biodegradation of chemicals tested in mixtures and individually: mixture effects on biodegradation kinetics and microbial composition - Heidi Birch; Arnaud Dechesne; Karina Knudsmark Sjøholm; Philipp Mayer (Biodegradation, 2023)

Biodegradation of organic compounds in the coal gangue by Bacillus sp. into humic acid - Chenxu Liu; Shuhua Ma; Xiaohui Wang; Yanjun Ou; Hao Du (Biodegradation, 2023)

Goethite and riboflavin synergistically enhance Cr(VI) reduction by Shewanella oneidensis MR-1 - Yaqin Zheng; Haibo Li; Yue Yang; Bingze Wu; Xinjing Li; Kaixuan Wang; Pengkai Wang; Chenxi Zhang (Biodegradation, 2023)

Enhanced bioremediation of oil-contaminated soil in a slurry bioreactor by H2O2-stimulation of oil-degrading/biosurfactant-generating bacteria: performance optimization and bacterial metagenomics - Farzaneh Fanaei; Gholamreza Moussavi; Sakine Shekoohiyan (Biodegradation, 2023)

Bioremediation of heavy oily sludge: a microcosms study - Cinthya Rondon-Afanador; Gustavo Pinilla-Meza; Francy C. Casallas-Cuervo; Camila Diaz-Vanegas; Daniela Barreto-Gomez; Carolina Benavides; Nicole Buitrago; Melissa Calvo; Camila Forero-Forero; Valentina Galvis-Ibarra; Victoria Moscoso-Urdaneta; Maria C. Perdomo-Rengifo; et al. (Biodegradation, 2023)
Oily sludge is a residue from the petroleum industry composed of a mixture of sand, water, metals, and high content of hydrocarbons (HCs). The heavy oily sludge used in this study originated from Colombian crude oil with high density and low American Petroleum Institute (API) gravity. The residual waste from heavy oil processing was subject to thermal and centrifugal extraction, resulting in heavy oily sludge with very high density and viscosity. Biodegradation of the total petroleum hydrocarbons (TPH) was tested in microcosms using several bioremediation approaches, including: biostimulation with bulking agents and nutrients, the surfactant Tween 80, and bioaugmentation. Select HC degrading bacteria were isolated based on their ability to grow and produce clear zones on different HCs. Degradation of TPH in the microcosms was monitored gravimetrically and with gas chromatography (GC). The TPH removal in all treatments ranged between 2 and 67%, regardless of the addition of microbial consortiums, amendments, or surfactants within the tested parameters. The results of this study demonstrated that bioremediation of heavy oily sludge presents greater challenges to achieve regulatory requirements. Additional physicochemical treatments analysis to remediate this recalcitrant material may be required to achieve a desirable degradation rate.

Aerobic degradation of bisphenol A by Pseudomonas sp. LM-1: characteristic and pathway - Chen Gu; Jinxuan Liang; Ming Liu; Jianliang Rui; Jiyan Shi; Yanming Yu; Xuwang Zhang (Biodegradation, 2023)

Role of pine needle biochar in operation and stability of anaerobic processes - Chander Mohan; Ajit Annachhatre (Biodegradation, 2023)

Optimized culture conditions facilitate the estrone biodegradation ability and laccase activity of Spirulina CPCC-695 - Neha Sami; Tasneem Fatma (Biodegradation, 2023)

Evaluation of aerobic biodegradation of phenanthrene using Pseudomonas turukhanskensis: an optimized study - Monika Sharma; El-Sayed Salama; Muhammad Usman; Aman Khan; Muhammad Arif; Xiangkai Li (Biodegradation, 2023)

Myco-degradation of microplastics: an account of identified pathways and analytical methods for their determination - Swati Solanki; Surbhi Sinha; Rachana Singh (Biodegradation, 2022)

Applying the 15N labelling technique to material derived from a landfill simulation experiment to understand nitrogen cycle processes under aerobic and anaerobic conditions - Nora Fricko; Wolfgang Wanek; Johann Fellner (Biodegradation, 2022)
Reactive nitrogen (N) species, such as ammonium (NH 4 + ), nitrate (NO 3 ) and gaseous nitrous oxide (N 2 O), are released into the environment during the degradation of municipal solid waste (MSW), causing persistent environmental problems. Landfill remediation measures, such as in-situ aeration, may accelerate the degradation of organic compounds and reduce the discharge of ammonium via leachate. Nonetheless, the actual amount of N in the waste material remains relatively constant and a coherent explanation for the decline in leachate ammonium concentrations is still lacking. Hence, the present study aimed to elucidate the dynamics of N and its transformation processes during waste degradation. To this end, the gross rates of organic N mineralization and nitrification were measured using 15 N pool dilution in waste material derived from a landfill simulation reactor (LSR) experiment. The results revealed a high potential for N mineralization and nitrification, the latter of which declined with the diminishing amount of extractable ammonium (after aeration). The analysis of the concentration and isotopic composition of N 2 O formed confirmed incomplete denitrification as the main source for N 2 O. Moreover, the natural abundance of 15 N was investigated in various waste N pools to verify the conclusions drawn from the 15 N tracing experiment. δ 15 N values of total waste N increased during aeration, indicating that nitrification is the major driver for N losses from aerated waste. The application of stable isotopes thereby allowed unprecedented insights into the complex N dynamics in decomposing landfill waste, of their response to aeration and their effect on hydrological versus gaseous loss pathways.

Enhanced biodegradation of oil-contaminated soil oil in shale gas exploitation by biochar immobilization - Hongyang Ren; Yuanpeng Deng; Liang Ma; Zijing Wei; Lingli Ma; Demin Yang; Bing Wang; Zheng-Yu Luo (Biodegradation, 2022)

Biodegradation of rubber in cultures of Rhodococcus rhodochrous and by its enzyme latex clearing protein - Rodrigo Andler; Camila Guajardo; Catalina Sepúlveda; Valentina Pino; Vilma Sanhueza; Vivian D’Afonseca (Biodegradation, 2022)

Bacillus pumilus proteome changes in response to 2,4,6-trinitrotoluene-induced stress - Galina Yakovleva; William Kurdy; Anna Gorbunova; Irina Khilyas; Guenter Lochnit; Olga Ilinskaya (Biodegradation, 2022)

Efficient bioremediation of PAHs-contaminated soils by a methylotrophic enrichment culture - Kartik Dhar; Logeshwaran Panneerselvan; Kadiyala Venkateswarlu; Mallavarapu Megharaj (Biodegradation, 2022)
Bioaugmentation effectively enhances microbial bioremediation of hazardous polycyclic aromatic hydrocarbons (PAHs) from contaminated environments. While screening for pyrene-degrading bacteria from a former manufactured gas plant soil (MGPS), the mixed enrichment culture was found to be more efficient in PAHs biodegradation than the culturable pure strains. Interestingly, analysis of 16S rRNA sequences revealed that the culture was dominated by a previously uncultured member of the family Rhizobiaceae . The culture utilized C1 and other methylotrophic substrates, including dimethylformamide (DMF), which was used as a solvent for supplementing the culture medium with PAHs. In the liquid medium, the culture rapidly degraded phenanthrene, pyrene, and the carcinogenic benzo(a)pyrene (BaP), when provided as the sole carbon source or with DMF as a co-substrate. The efficiency of the culture in the bioremediation of PAHs from the MGPS and a laboratory waste soil (LWS) was evaluated in bench-scale slurry systems. After 28 days, 80% of Σ16 PAHs were efficiently removed from the inoculated MGPS. Notably, the bioaugmentation achieved 90% removal of four-ringed and 60% of highly recalcitrant five- and six-ringed PAHs from the MGPS. Likewise, almost all phenanthrene, pyrene, and 65% BaP were removed from the bioaugmented LWS. This study highlights the application of the methylotrophic enrichment culture dominated by an uncultured bacterium for the efficient bioremediation of PAHs.

Composite hydrolytic acidification - aerobic MBBR process for treating traditional Chinese medicine wastewater - Likun Huang; Zhe Li; Guangzhi Wang; Jingfu Han; Yue Hou; Ning Zhang (Biodegradation, 2022)

Laccase-loaded magnetic dialdehyde inulin nanoparticles as an efficient heterogeneous natural polymer-based biocatalyst for removal and detoxification of ofloxacin - Mahtab Shokri; Somayeh Mojtabavi; Hossein Jafari-Nodoushan; Khashayar Vojdanitalab; Shiva Golshani; Hoda Jahandar; Mohammad Ali Faramarzi (Biodegradation, 2022)

Comparison of polylactic acid biodegradation ability of Brevibacillus brevis and Bacillus amyloliquefaciens and promotion of PLA biodegradation by soytone - Jaeyoung Yu; Pa Do Kim; Yewon Jang; Sung-Kon Kim; Jeehoon Han; Jiho Min (Biodegradation, 2022)

Investigating the effect of nanoparticle on phenanthrene biodegradation by Labedella gwakjiensis strain KDI - Maryam Firoozbakht; Abbas Akhavan Sepahi; Hamid Rashedi; Fatemeh Yazdian (Biodegradation, 2022)

Degradation characteristics of crude oil by a consortium of bacteria in the existence of chlorophenol - Jing Li; Qiyou Liu; Shuo Sun; Xiuxia Zhang; Xiuying Zhao; Junlong Yu; Wu Cui; Yi Du (Biodegradation, 2022)

Optimizing the malathion degrading potential of a newly isolated Bacillus sp. AGM5 based on Taguchi design of experiment and elucidation of degradation pathway - Mohd Ashraf Dar; Garima Kaushik (Biodegradation, 2022)

Soot biodegradation by psychrotolerant bacterial consortia - Barkat Ali; Wasim Sajjad; Nikhat Ilahi; Ali Bahadur; Shichang Kang (Biodegradation, 2022)

Concerted action of extracellular and cytoplasmic esterase and urethane-cleaving activities during Impranil biodegradation by Alicycliphilus denitrificans BQ1 - Jacqueline Fuentes-Jaime; Martín Vargas-Suárez; M. Javier Cruz-Gómez; Herminia Loza-Tavera (Biodegradation, 2022)

A multi-step nitrifying microbial enrichment to remove ammonia and nitrite in brackish aquaculture systems - Dao Thi Anh Tuyet; Le Minh Hiep; Ha Thi Binh; Le Thanh Huyen; Sen-Lin Tang; Pei-Wen Chiang; Do Manh Hao (Biodegradation, 2022)

Single-well push–pull tests evaluating isobutane as a primary substrate for promoting in situ cometabolic biotransformation reactions - Hannah Rolston; Michael Hyman; Lewis Semprini (Biodegradation, 2022)

Vinasse odyssey: sugarcane vinasse remediation and laccase production by Trametes sp. immobilized in polyurethane foam - Pablo M. Ahmed; Carlos G. Nieto-Peñalver; Lucía I. C. de Figueroa; Hipólito F. Pajot (Biodegradation, 2022)

The intrinsic relevance of nitrogen removal pathway to varying nitrate loading rate in a polycaprolactone-supported denitrification system - Shiyang Zhang; Zhiwei Tang; Shibin Xia; Yinghe Jiang; Meng Li; Bing Wang (Biodegradation, 2022)

Degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) by fungi originating from Vietnam - Thi Lan Anh Nguyen; Anh Thi Ngoc Dao; Ha Thi Cam Dang; Jacco Koekkoek; Abraham Brouwer; Tjalf E. de Boer; Rob J. M. van Spanning (Biodegradation, 2022)
Three different fungi were tested for their ability to degrade 2,4-dichlorophenoxyacetic acid and 2,4,5-trichlorophenoxyacetic acid and for the role of laccases and cytochromes P450-type in this process. We studied a white-rot fungus Rigidoporus sp. FMD21, which has a high laccase activity, for its efficiency to degrade these herbicides. A positive correlation was found between its laccase activity and the corresponding herbicide degradation rate. Even more, the doubling of the enzyme activity in this phase corresponded with a doubling of the herbicide degradation rate. It is, therefore, tempting to speculate that laccase is the most dominant enzyme in the degradation of 2,4-D and 2,4,5-T under these conditions. In addition, it was shown that Rigidoporus sp. FMD21 partly relies on cytochromes P450-type for the breakdown of the herbicides as well. Two filamentous fungi were isolated from soil contaminated with herbicides and dioxins located at Bien Hoa airbase. They belong to genera Fusarium and Verticillium of the phylum Ascomycota as judged by their 18S rRNA gene sequences. Both isolated fungi were able to degrade the herbicides but with different rates. Their laccase activity, however, was very low and did not correlate with the rate of breakdown of the herbicides. These data indicate that the white-rot fungus most likely synthesizes laccase and cytochromes P450-type for the breakdown of the herbicides, while the types of enzyme used for the breakdown of the herbicides by the two Ascomycota remain unclear. Graphical abstract

Tylosin in anaerobic reactors: degradation kinetics, effects on methane production and on the microbial community - Aline Gomes de Oliveira Paranhos; Andressa Rezende Pereira; Yasmim Arantes da Fonseca; Silvana de Queiroz Silva; Sérgio Francisco de Aquino (Biodegradation, 2022)

Investigating the biological degradation of the drug β-blocker atenolol from wastewater using the SBR - Reza Rezaei; Ali Ahmad Aghapour; Hassan Khorsandi (Biodegradation, 2022)

Enhanced anaerobic treatment of synthetic protein-rich wastewater promoted by organic xerogels - Luis A. Ramírez-Montoya; Miguel A. Montes-Morán; J. Rene Rangel-Mendez; Francisco J. Cervantes (Biodegradation, 2022)

Up-flow anaerobic column reactor for sulfate-rich cadmium-bearing wastewater purification: system performance, removal mechanism and microbial community structure - Yongrong Jiang; Jie Zhang; Qianmin Wen; Junjian Zheng; Yuanyuan Zhang; Qiaoyan Wei; Yongli Qin; Xuehong Zhang (Biodegradation, 2022)

Immobilization of psychrophile Psychrobacter sp. ANT206 onto novel reusable magnetic nanoparticles and its application for nitro-aromatic compounds biodegradation under low temperature - Yifan Wang; Yanhua Hou; Yatong Wang; Ailin Zhang; Quanfu Wang (Biodegradation, 2022)

Kinetic isotope effects of C and N indicate different transformation mechanisms between atzA- and trzN-harboring strains in dechlorination of atrazine - Songsong Chen; Limin Ma; Yuncai Wang (Biodegradation, 2022)

Hydrocarbon-degrading bacteria in Colombia: systematic review - Diana Carolina Rache-Arce; Maryuris Machacado-Salas; Doris Rosero-García (Biodegradation, 2022)
Petroleum industry activities worldwide have caused pollution and resulted in environmental degradation. Microorganisms with the potential to reduce pollutant levels by degradation processes have been reported, and bacteria are among such organisms. The first study on bacterial degradation in Colombia was published in 1996. The study isolated bacteria belonging to the Pseudomonas genus from hydrocarbon-polluted sediments. Since then, different reports on degrading bacteria have been published. The objective of this systematic review is to identify and analyze all the studies on hydrocarbon-degrading bacteria performed in Colombia. To accomplish this goal, a literature search was conducted. Inclusion and exclusion criteria were applied, and 37 relevant articles were obtained. We found that 2018 was the year with the largest number of publications in Colombia, and most frequently identified bacterial genera were Pseudomonas and Bacillus . Some studies showed that the degradation of hydrocarbons is more efficient when bacterial consortia are used rather than pure cultures. This study provides information about bacteria with the potential to degrade hydrocarbons in Colombia, which in turn will be a source of information for future studies in this field.

Co-metabolism of nonylphenol ethoxylate in sequencing batch reactor under aerobic conditions - Alpaslan Ekdal; Didem Okutman Tas; Gulsum Emel Zengin; Irmak Batı Onay; Tugba Olmez Hanci; Derin Orhon; Emine Cokgor (Biodegradation, 2022)

Biological recovery of phosphorus from waste activated sludge via alkaline fermentation and struvite biomineralization by Brevibacterium antiquum - Sevil Coşgun; Büşra Kara; Büşra Kunt; Ceren Hür; Neslihan Semerci (Biodegradation, 2022)

Performance analysis of Pseudomonas sp. strain SA3 in naphthalene degradation using phytotoxicity and microcosm studies - Sushma Rani Tirkey; Shristi Ram; Madhusree Mitra; Sandhya Mishra (Biodegradation, 2022)

Effect of biostimulation and bioaugmentation on biodegradation of high concentrations of 1,4-dioxane - Ángel A. Ramos-García; Claudia Walecka-Hutchison; David L. Freedman (Biodegradation, 2022)

Evaluation of in situ biosurfactant production by inoculum of P. putida and nutrient addition for the removal of polycyclic aromatic hydrocarbons from aged oil-polluted soil - Ángeles Martínez-Toledo; María del Carmen Cuevas-Díaz; Owsaldo Guzmán-López; Jaime López-Luna; César Ilizaliturri-Hernández (Biodegradation, 2022)

LcaR: a regulatory switch from Pseudomonas aeruginosa for bioengineering alkane degrading bacteria - Erandika H. Hemamali; Laksiri P. Weerasinghe; Hideaki Tanaka; Genji Kurisu; Inoka C. Perera (Biodegradation, 2022)

Alterations of endophytic microbial community function in Spartina alterniflora as a result of crude oil exposure - Samantha D. Addis; Stephen K. Formel; Yeon Ji Kim; Paige B. Varner; Daniel B. Raudabaugh; Emilie Lefevre; Brittany M. Bernik; Vijaikrishnah Elango; Sunshine A. Van Bael; John H. Pardue; Claudia K. Gunsch (Biodegradation, 2022)

A critical review on microbial degradation of petroleum-based plastics: quantitatively effects of chemical addition in cultivation media on biodegradation efficiency - Yong Sun; Jing Hu; Abubakar Yusuf; Yixiao Wang; Huan Jin; Xiyue Zhang; Yiyang Liu; Yunshan Wang; Gang Yang; Jun He (Biodegradation, 2022)

The microbial metabolic activity on carbohydrates and polymers impact the biodegradability of landfilled solid waste - Christian Brandstaetter; Nora Fricko; Mohammad J. Rahimi; Johann Fellner; Wolfgang Ecker-Lala; Irina S. Druzhinina (Biodegradation, 2022)
Biological waste degradation is the main driving factor for landfill emissions. In a 2-year laboratory experiment simulating different landfill in-situ aeration scenarios, the microbial degradation of solid waste under different oxygen conditions (treatments) was investigated. Nine landfill simulation reactors were operated in triplicates under three distinct treatments. Three were kept anaerobic, three were aerated for 706 days after an initial anaerobic phase and three were aerated for 244 days in between two anaerobic phases. In total, 36 solid and 36 leachate samples were taken. Biolog® EcoPlates™ were used to assess the functional diversity of the microbial community. It was possible to directly relate the functional diversity to the biodegradability of MSW (municipal solid waste), measured as RI 4 (respiration index after 4 days). The differences between the treatments in RI 4 as well as in carbon and polymer degradation potential were small. Initially, a RI 4 of about 6.5 to 8 mg O 2 kg −1 DW was reduced to less than 1 mg O 2 kg −1 DW within 114 days of treatment. After the termination of aeration, an increase 3 mg O 2 kg −1 DW was observed. By calculating the integral of the Gompertz equation based on spline interpolation of the Biolog® EcoPlates™ results after 96 h two substrate groups mainly contributing to the biodegradability were identified: carbohydrates and polymers. The microbial activity of the respective microbial consortium could thus be related to the biodegradability with a multilinear regression model.

Degradation of dibutyl phthalate by Paenarthrobacter sp. Shss isolated from Saravan landfill, Hyrcanian Forests, Iran - S. Shariati; C. Ebenau-Jehle; A. A. Pourbabaee; H. A. Alikhani; M. Rodriguez-Franco; M. Agne; M. Jacoby; R. Geiger; F. Shariati; M. Boll (Biodegradation, 2022)
Phthalic acid esters are predominantly used as plasticizers and are industrially produced on the million ton scale per year. They exhibit endocrine-disrupting, carcinogenic, teratogenic, and mutagenic effects on wildlife and humans. For this reason, biodegradation, the major process of phthalic acid ester elimination from the environment, is of global importance. Here, we studied bacterial phthalic acid ester degradation at Saravan landfill in Hyrcanian Forests, Iran, an active disposal site with 800 tons of solid waste input per day. A di- n -butyl phthalate degrading enrichment culture was established from which Paenarthrobacter sp. strain Shss was isolated. This strain efficiently degraded 1 g L –1 di- n -butyl phthalate within 15 h with a doubling time of 5 h. In addition, dimethyl phthalate, diethyl phthalate, mono butyl phthalate, and phthalic acid where degraded to CO 2 , whereas diethyl hexyl phthalate did not serve as a substrate. During the biodegradation of di- n -butyl phthalate, mono- n -butyl phthalate was identified in culture supernatants by ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry. In vitro assays identified two cellular esterase activities that converted di- n -butyl phthalate to mono- n -butyl phthalate, and the latter to phthalic acid, respectively. Our findings identified Paenarthrobacter sp. Shss amongst the most efficient phthalic acid esters degrading bacteria known, that possibly plays an important role in di- n -butyl phthalate elimination at a highly phthalic acid esters contaminated landfill.

Achieving high-rate partial nitritation with aerobic granular sludge at low temperatures - Wenru Liu; Yaoliang Shen; Dianhai Yang (Biodegradation, 2022)

Biosorption of nickel, cobalt, zinc and copper ions by Serratia marcescens strain 16 in mono and multimetallic systems - A. Díaz; J. Marrero; G. Cabrera; O. Coto; J. M. Gómez (Biodegradation, 2022)
The metallurgical industry is one of the main sources of heavy metal pollution, which represents a severe threat to life. Metals can be removed from aqueous solutions by using microbial biomasses. This paper analyses the heavy metal biosorption capacity of Serratia marcescens strain 16 in single and multimetallic systems. The results obtained show that Co(II), Ni(II) and Zn(II) biosorption in monometallic systems is two to three times higher than in the presence of bi-metallic and multimetallic solutions. Fourier transform infrared spectroscopy confirmed that carbonyl, carboxyl and hydroxyl were the main functional groups, as well as the amide bands I and II involved in metal uptake, which are present in external structures of the bacterial cell. The results obtained demonstrated the viability of S. marcescens strain 16 as a biosorbent for the design of eco-friendly technologies for the treatment of waste liquor.

Biodegradation of 4-hydroxybenzoic acid by Acinetobacter johnsonii FZ-5 and Klebsiella oxytoca FZ-8 under anaerobic conditions - Peng Lu; Huiying Huang; Yixiao Sun; Mengyao Qiang; Yan Zhu; Mengjiao Cao; Xue Peng; Bo Yuan; Zhaozhong Feng (Biodegradation, 2022)