Heterologous gene expression and characterization of two serine hydroxymethyltransferases from Thermoplasma acidophilum - Ilma Fauziah Ma’ruf; Yuka Sasaki; Anastasia Kerbs; Jochen Nießer; Yu Sato; Hironori Taniguchi; Kenji Okano; Shigeru Kitani; Elvi Restiawaty; Akhmaloka; Kohsuke Honda (Extremophiles, 2021)

Glutamate optimizes enzymatic activity under high hydrostatic pressure in Desulfovibrio species: effects on the ubiquitous thioredoxin system - H. Gaussier; M. Nouailler; E. Champaud; E. B. Garcin; C. Sebban-Kreuzer; O. Bornet; M. Garel; C. Tamburini; L. Pieulle; A. Dolla; N. Pradel (Extremophiles, 2021)

Transcriptome and growth efficiency comparisons of recombinant thermophiles that produce thermolabile and thermostable proteins: implications for burden-based selection of thermostable proteins - Hirokazu Suzuki; Yuta Okumura; Yui Mikawa; Mao Takata; Shunsuke Yoshimura; Takashi Ohshiro (Extremophiles, 2021)

The largest moss carpet transplant in Antarctica and its bryosphere cryptic biodiversity - Paulo E. A. S. Câmara; Peter Convey; Sandro B. Rangel; Marcelo Konrath; Cristine Chaves Barreto; Otavio H. B. Pinto; Micheline Carvalho Silva; Diego Knop Henriques; Hermeson Cassiano de Oliveira; Luiz H. Rosa (Extremophiles, 2021)

Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048 - Giacomo Fais; Veronica Malavasi; Paola Scano; Santina Soru; Pierluigi Caboni; Giacomo Cao (Extremophiles, 2021)
With an unsupervised GC–MS metabolomics approach, polar metabolite changes of the microalgae Coccomyxa melkonianii SCCA 048 grown under standard conditions for seven weeks were studied. C. melkonianii was sampled at the Rio Irvi River, in the mining site of Montevecchio-Ingurtosu (Sardinia, Italy), which is severely contaminated by heavy metals and shows high concentrations of sulfates. The partial-least-square (PLS) analysis of the GC–MS data indicated that growth of C. melkonianii was characterized by an increase of the levels of threonic acid, myo -inositol, malic acid, and fumaric acid. Furthermore, at the sixth week of exponential phase the lipid fingerprint of C. melkonianii was studied by LC-QTOF-MS. C. melkonianii lipid extract characterized through an iterative MS/MS analysis showed the following percent levels: 61.34 ± 0.60% for triacylglycerols (TAG); 11.55 ± 0.09% for diacylglyceryltrimethyl homoserines (DGTS), 11.34 ± 0.10% for sulfoquinovosyldiacylglycerols (SQDG) and, 5.29 ± 0.04% for lysodiacylglyceryltrimethyl homoserines (LDGTS). Noteworthy, we were able to annotate different fatty acid ester of hydroxyl fatty acid, such as FAHFA (18:1_20:3), FAHFA (18:2_20:4), FAHFA (18:0_20:2), and FAHFA (18:1_18:0), with relevant biological activity. These approaches can be useful to study the biochemistry of this extremophile algae in the view of its potential exploitation in the phycoremediation of polluted mining areas.

Characterization of the DdrD protein from the extremely radioresistant bacterium Deinococcus radiodurans - Claire Bouthier de la Tour; Martine Mathieu; Pascale Servant; Geneviève Coste; Cédric Norais; Fabrice Confalonieri (Extremophiles, 2021)
Here, we report the in vitro and in vivo characterization of the DdrD protein from the extraordinary stress-resistant bacterium, D. radiodurans . DdrD is one of the most highly induced proteins following cellular irradiation or desiccation. We confirm that DdrD belongs to the Radiation Desiccation Response (RDR) regulon protein family whose expression is regulated by the IrrE/DdrO proteins after DNA damage. We show that DdrD is a DNA binding protein that binds to single-stranded DNA In vitro, but not to duplex DNA unless it has a 5′ single-stranded extension. In vivo, we observed no significant effect of the absence of DdrD on the survival of D. radiodurans cells after exposure to γ-rays or UV irradiation in different genetic contexts. However, genome reassembly is affected in a ∆ddrD mutant when cells recover from irradiation in the absence of nutrients. Thus, DdrD likely contributes to genome reconstitution after irradiation, but only under starvation conditions. Lastly, we show that the absence of the DdrD protein partially restores the frequency of plasmid transformation of a ∆ddrB mutant, suggesting that DdrD could also be involved in biological processes other than the response to DNA damage.

Prokaryotic diversity and biogeochemical characteristics of field living and laboratory cultured stromatolites from the hypersaline Laguna Interna, Salar de Atacama (Chile) - Jorge R. Osman; Pabla Viedma; Jorge Mendoza; Gustavo Fernandes; Michael S. DuBow; Davor Cotoras (Extremophiles, 2021)

IND-enzymes: a repository for hydrolytic enzymes derived from thermophilic and psychrophilic bacterial species with potential industrial usage - Jithin S. Sunny; Khairun Nisha; Anuradha Natarajan; Lilly M. Saleena (Extremophiles, 2021)

A comparative analysis of gaseous phase hydration properties of two lichenized fungi: Niebla tigrina (Follman) Rundel & Bowler from Atacama Desert and Umbilicaria antarctica Frey & I. M. Lamb from Robert Island, Southern Shetlands Archipelago, maritime Antarctica - Hubert Harańczyk; K. Strzałka; K. Kubat; A. Andrzejowska; M. Olech; D. Jakubiec; P. Kijak; G. Palfner; Angélica Casanova-Katny (Extremophiles, 2021)
Gaseous phase hydration properties for thalli of Niebla tigrina from Atacama Desert, and for Umbilicaria antarctica from Isla Robert, maritime Antarctica, were analyzed using 1 H-NMR relaxometry, spectroscopy, and sorption isotherm analysis. The molecular dynamics of residual water was monitored to distinguish the sequential binding very tightly, tightly, and loosely bound water fractions. These two species differ in hydration kinetics faster for Desert N. tigrina [ A 1 = 0.51(4); t 1 = 0.51(5) h, t 2 = 15.0(1.9) h; total 0.7 for p / p 0 = 100%], compared to Antarctic U. antarctica [ A 1 = 0.082(6), t 1 = 2.4(2) h, t 2 = [26.9(2.7)] h, total 0.6 for p / p 0 = 100%] from humid polar area. The 1 H-NMR measurements distinguish signal from tightly bound water, and two signals from loosely bound water, with different chemical shifts higher for U. antarctica than for N. tigrina . Both lichen species contain different amounts of water-soluble solid fraction. For U. antarctica, the saturation concentration of water soluble solid fraction, c s = 0.55(9), and the dissolution effect is detected at least up to Δ m / m 0 = 0.7, whereas for N. tigrina with the similar saturation concentration, c s = 053(4), this fraction is detected up to the threshold hydration level equal to Δ M/m 0 = 0.3 only.

Synthesis of fucosylated oligosaccharides with α-l-fucosidase from Thermotoga maritima immobilized on Eupergit® CM - Francisco Guzmán-Rodríguez; Sergio Alatorre-Santamaría; Lorena Gómez-Ruiz; Gabriela Rodríguez-Serrano; Mariano García-Garibay; Alma Cruz-Guerrero (Extremophiles, 2021)

Comparison of picoeukaryote community structures and their environmental relationships between summer and autumn in the southern Chukchi Sea - Fang Zhang; Jianfeng He; Haiyan Jin; Qiang Hao; Zhongyong Gao; Heng Sun (Extremophiles, 2021)

Anaerotalea alkaliphila gen. nov., sp. nov., an alkaliphilic, anaerobic, fermentative bacterium isolated from a terrestrial mud volcano - Anastasia Frolova; A. Yu. Merkel; A. A. Novikov; E. A. Bonch-Osmolovskaya; A. I. Slobodkin (Extremophiles, 2021)

Variation of salinity and nitrogen concentration affects the pentacyclic triterpenoid inventory of the haloalkaliphilic aerobic methanotrophic bacterium Methylotuvimicrobium alcaliphilum - Alexmar Cordova-Gonzalez; Daniel Birgel; Andreas Kappler; Jörn Peckmann (Extremophiles, 2021)
The occurrence and activity of aerobic methanotrophs are influenced by environmental conditions, including pH, temperature, salinity, methane and oxygen concentrations, and nutrient availability. Aerobic methanotrophs synthesize a variety of lipids important for cell functions. However, culture-based experiments studying the influence of environmental parameters on lipid production by aerobic methanotrophs are scarce. Such information is crucial to interpret lipid patterns of methanotrophic bacteria in the environment. In this study, the alkaliphilic strain Methylotuvimicrobium alcaliphilum was cultivated under different salinities and different nitrate concentrations to assess the effect of changing conditions on the inventory of pentacyclic triterpenoids. The results indicate that hopanoid abundance is enhanced at lower salinity and higher nitrate concentration. The production of most pentacyclic triterpenoids was favored at low salinity, especially for aminotriol. Interestingly, 3-methyl-aminotetrol and tetrahymanol were favored at higher salinity. Bacteriohopanepolyols (BHPs), particularly aminotriol and 3-methyl-aminotriol, increased considerably at higher nitrate concentrations. Four novel N -containing BHPs—aminodiol, 3-methyl-aminodiol, and isomers of aminotriol and 3-methyl-aminotriol—were identified. This study highlights the significance of environmental factors for bacterial lipid production and documents the need for cultivation studies under variable conditions to utilize the full potential of the biomarker concept.

Diversity of fungal DNA in lake sediments on Vega Island, north-east Antarctic Peninsula assessed using DNA metabarcoding - Mayara Baptistucci Ogaki; Paulo Eduardo Aguiar Saraiva Câmara; Otávio Henrique Bezerra Pinto; Juan Manuel Lirio; Silvia H. Coria; Rosemary Vieira; Micheline Carvalho-Silva; Peter Convey; Carlos Augusto Rosa; Luiz Henrique Rosa (Extremophiles, 2021)

The hyperthermophilic archaeon Pyrococcus furiosus utilizes environmental iron sulfide cluster complexes as an iron source - Sonya M. Clarkson; Dominik K. Haja; Michael W. W. Adams (Extremophiles, 2021)

Role of extremophiles and their extremozymes in biorefinery process of lignocellulose degradation - Dixita Chettri; Ashwani Kumar Verma; Lija Sarkar; Anil Kumar Verma (Extremophiles, 2021)

Molecular strategies to enhance stability and catalysis of extremophile-derived α-amylase using computational biology - Nisha Gupta; Esmil Beliya; Jai Shankar Paul; Shubhra Tiwari; Shriram Kunjam; Shailesh Kumar Jadhav (Extremophiles, 2021)

Fungal diversity present on rocks from a polar desert in continental Antarctica assessed using DNA metabarcoding - Graciéle Cunha Alves de Menezes; Paulo E. A. S. Câmara; Otávio Henrique Bezerra Pinto; Micheline Carvalho-Silva; Fábio Soares Oliveira; Caroline Delpupo Souza; Carlos Ernesto G. Reynaud Schaefer; Peter Convey; Carlos Augusto Rosa; Luiz Henrique Rosa (Extremophiles, 2021)

Antarctic lichens as a source of phosphate-solubilizing bacteria - Averlane Vieira da Silva; Adeildo Junior de Oliveira; Ithallo Sathio Bessoni Tanabe; José Vieira Silva; Tiago Wallace da Silva Barros; Mayanne Karla da Silva; Paulo Henrique Barcellos França; Jakson Leite; Jair Putzke; Rosalinda Montone; Valéria Maia de Oliveira; Luiz Henrique Rosa; et al. (Extremophiles, 2021)

Halo(natrono)archaea from hypersaline lakes can utilize sulfoxides other than DMSO as electron acceptors for anaerobic respiration - Dimitry Y. Sorokin; Pawel Roman; Tatjana V. Kolganova (Extremophiles, 2021)

RNA transcript response by an Acidithiobacillus spp. mixed culture reveals adaptations to growth on arsenopyrite - Carlos Eduardo Barragán; Marco Antonio Márquez; Mark Dopson; Dolly Montoya (Extremophiles, 2021)

Cultivation and characterization of snowbound microorganisms from the South Pole - Mackenzie K. Hayward; Emma D. Dewey; Kathryn N. Shaffer; Austin M. Huntington; Brad M. Burchell; Lynn M. Stokes; Brittney C. Alexander; Janessa E. George; Megan L. Kempher; Samantha B. Joye; Michael T. Madigan; W. Matthew Sattley (Extremophiles, 2021)

Characterization of a carboxylesterase with hyper-thermostability and alkali-stability from Streptomyces lividans TK24 - Xin Chang; Shuang Wu; Jie Chen; Shengqi Xiong; Peng Wang; Xueqin Shi; Ao Wang; Baojuan Wang (Extremophiles, 2021)

Selection and re-acclimation of bioprospected acid-tolerant green microalgae suitable for growth at low pH - Sabrina Marie Desjardins; Corey Alfred Laamanen; Nathan Basiliko; John Ashley Scott (Extremophiles, 2021)

A multi-omic screening approach for the discovery of thermoactive glycoside hydrolases - Philip Busch; Marcel Suleiman; Christian Schäfers; Garabed Antranikian (Extremophiles, 2021)

Assessment of fungal diversity present in lakes of Maritime Antarctica using DNA metabarcoding: a temporal microcosm experiment - Láuren M. D. de Souza; Mayara B. Ogaki; Paulo E. A. S. Câmara; Otávio H. B. Pinto; Peter Convey; Micheline Carvalho-Silva; Carlos A. Rosa; Luiz H. Rosa (Extremophiles, 2021)

Temperature and elemental sulfur shape microbial communities in two extremely acidic aquatic volcanic environments - Diego Rojas-Gätjens; Alejandro Arce-Rodríguez; Fernando Puente-Sánchez; Roberto Avendaño; Eduardo Libby; Raúl Mora-Amador; Keilor Rojas-Jimenez; Paola Fuentes-Schweizer; Dietmar H. Pieper; Max Chavarría (Extremophiles, 2021)

Diversity analysis of thermophilic hydrogenogenic carboxydotrophs by carbon monoxide dehydrogenase amplicon sequencing using new primers - Kimiho Omae; Tatsuki Oguro; Masao Inoue; Yuto Fukuyama; Takashi Yoshida; Yoshihiko Sako (Extremophiles, 2021)
The microbial H 2 -producing (hydrogenogenic) carbon monoxide (CO)-oxidizing activity by the membrane-associated CO dehydrogenase (CODH)/energy-converting hydrogenase (ECH) complex is an important metabolic process in the microbial community. However, the studies on hydrogenogenic carboxydotrophs had to rely on inherently cultivation and isolation methods due to their rare abundance, which was a bottleneck in ecological study. Here, we provided gene-targeted sequencing method for the diversity estimation of thermophilic hydrogenogenic carboxydotrophs. We designed six new degenerate primer pairs which effectively amplified the coding regions of CODH genes forming gene clusters with ECH genes (CODHech genes) in Firmicutes which includes major thermophilic hydrogenogenic carboxydotrophs in terrestrial thermal habitats. Amplicon sequencing by these primers using DNAs from terrestrial hydrothermal sediments and CO-gas-incubated samples specifically detected multiple CODH genes which were identical or phylogenetically related to the CODHech genes in Firmictes. Furthermore, we found that phylogenetically distinct CODHech genes were enriched in CO-gas-incubated samples, suggesting that our primers detected uncultured hydrogenogenic carboxydotrophs as well. The new CODH-targeted primers provided us with a fine-grained (~ 97.9% in nucleotide sequence identity) diversity analysis of thermophilic hydrogenogenic carboxydotrophs by amplicon sequencing and will bolster the ecological study of these microorganisms.

Purification and characterization of two thermostable xylanases from a halotolerant Bacillus sp. Asc6BA isolated from Salar de Ascotán, Atacama Desert - Francisca Contreras; Maximiliano J. Amenabar; Jenny M. Blamey (Extremophiles, 2021)

Mechanism of TonB-dependent transport system in Halomonas alkalicola CICC 11012s in response to alkaline stress - Lei Zhai; Jiuyan Xie; Huijun Feng; Sijia Sun; Kun Cheng; Su Yao (Extremophiles, 2021)

Draft genome sequence of Promicromonospora panici sp. nov., a novel ionizing-radiation-resistant actinobacterium isolated from roots of the desert plant Panicum turgidum - Sihem Guesmi; Imen Nouioui; Petar Pujic; Audrey Dubost; Afef Najjari; Kais Ghedira; José M. Igual; Ameur Cherif; Hans-peter Klenk; Haïtham Sghaier; Philippe Normand (Extremophiles, 2021)

Effect of pH on the activity of ice-binding protein from Marinomonas primoryensis - Elizabeth A. Delesky; Patrick E. Thomas; Marimikel Charrier; Jeffrey C. Cameron; Wil V. Srubar (Extremophiles, 2021)

Ornithine carbamoyltransferase from psychrophiles to thermophiles: structural evolution of catalytic fold to accommodate physiological diversity - Shivika Sharma; Shikha Sharma; Mushtaq Ahmed; Yusuf Akhter; Subhankar Chatterjee (Extremophiles, 2021)

Genomic analysis of Caldalkalibacillus thermarum TA2.A1 reveals aerobic alkaliphilic metabolism and evolutionary hallmarks linking alkaliphilic bacteria and plant life - Samuel I. de Jong; Marcel A. van den Broek; Alexander Y. Merkel; Pilar de la Torre Cortes; Falk Kalamorz; Gregory M. Cook; Mark C. M. van Loosdrecht; Duncan G. G. McMillan (Extremophiles, 2020)
The aerobic thermoalkaliphile Caldalkalibacillus thermarum strain TA2.A1 is a member of a separate order of alkaliphilic bacteria closely related to the Bacillales order. Efforts to relate the genomic information of this evolutionary ancient organism to environmental adaptation have been thwarted by the inability to construct a complete genome. The existing draft genome is highly fragmented due to repetitive regions, and gaps between and over repetitive regions were unbridgeable. To address this, Oxford Nanopore Technology’s MinION allowed us to span these repeats through long reads, with over 6000-fold coverage. This resulted in a single 3.34 Mb circular chromosome. The profile of transporters and central metabolism gives insight into why the organism prefers glutamate over sucrose as carbon source. We propose that the deamination of glutamate allows alkalization of the immediate environment, an excellent example of how an extremophile modulates environmental conditions to suit its own requirements. Curiously, plant-like hallmark electron transfer enzymes and transporters are found throughout the genome, such as a cytochrome b 6 c 1 complex and a CO 2 -concentrating transporter. In addition, multiple self-splicing group II intron-encoded proteins closely aligning to those of a telomerase reverse transcriptase in Arabidopsis thaliana were revealed. Collectively, these features suggest an evolutionary relationship to plant life.

Investigation of lipid profile in Acetobacter pasteurianus Ab3 against acetic acid stress during vinegar production - Chengcheng Han; Kai Xia; Jieqiong Yang; Hong Zhang; Matthew P. DeLisa; Xinle Liang (Extremophiles, 2020)

Statistical and molecular dynamics (MD) simulation approach to investigate the role of intrinsically disordered regions of shikimate dehydrogenase in microorganisms surviving at different temperatures - Damodaran Kamalesh; Aditya Nair; Jaya Sreeshma; Pattathil Sasikumar Arundhathi; Chinnappan Sudandiradoss (Extremophiles, 2020)

Soil fungal community composition differs significantly among the Antarctic, Arctic, and Tibetan Plateau - Tao Zhang; Nengfei Wang; Liyan Yu (Extremophiles, 2020)

Biodegradation of organophosphorus pesticide chlorpyrifos by Sphingobacterium sp. C1B, a psychrotolerant bacterium isolated from apple orchard in Himachal Pradesh of India - Shalini Verma; Dharam Singh; Subhankar Chatterjee (Extremophiles, 2020)

A (6-4)-photolyase from the Antarctic bacterium Sphingomonas sp. UV9: recombinant production and in silico features - Juan José Marizcurrena; Tilman Lamparter; Susana Castro-Sowinski (Extremophiles, 2020)

Poorly known microbial taxa dominate the microbiome of hypersaline Sambhar Lake salterns in India - Srikanta Pal; Raju Biswas; Arijit Misra; Abhijit Sar; Sohini Banerjee; Puja Mukherjee; Bomba Dam (Extremophiles, 2020)

Undecane production by cold-adapted bacteria from Antarctica - Michel Rodrigo Zambrano Passarini; Tiago Rodrigues e Silva; Suzan Prado Fernandes Bernal; Nathália Luana Cecchet; Adilson Sartoratto; Marcela Boroski; Alysson Wagner Fernandes Duarte; Júlia Ronzella Ottoni; Luiz Henrique Rosa; Valéria Maia de Oliveira (Extremophiles, 2020)

Study on the osmoregulation of “Halomonas socia” NY-011 and the degradation of organic pollutants in the saline environment - Silun Peng; Min Kai; Xiaoyu Yang; Yanyun Luo; Linhan Bai (Extremophiles, 2020)

Prokaryotic diversity in stream sediments affected by acid mine drainage - Jorge D. Carlier; Sara Ettamimi; Cymon J. Cox; Khalil Hammani; Hassan Ghazal; Maria Clara Costa (Extremophiles, 2020)

Diversity and distribution of cultivable fungi present in acid sulphate soils in chronosequence under para-periglacial conditions in King George Island, Antarctica - Bárbara Alves Porto; Thamar Holanda da Silva; Mariana Resende Machado; Fábio Soares de Oliveira; Carlos Augusto Rosa; Luiz Henrique Rosa (Extremophiles, 2020)

Pentose degradation in archaea: Halorhabdus species degrade D-xylose, L-arabinose and D-ribose via bacterial-type pathways - Jan-Moritz Sutter; Ulrike Johnsen; Andreas Reinhardt; Peter Schönheit (Extremophiles, 2020)
The degradation of the pentoses d -xylose, l -arabinose and d -ribose in the domain of archaea, in Haloferax volcanii and in Haloarcula and Sulfolobus species, has been shown to proceed via oxidative pathways to generate α-ketoglutarate. Here, we report that the haloarchaeal Halorhabdus species utilize the bacterial-type non-oxidative degradation pathways for pentoses generating xylulose-5-phosphate. The genes of these pathways are each clustered and were constitutively expressed. Selected enzymes involved in d -xylose degradation, xylose isomerase and xylulokinase, and those involved in l -arabinose degradation, arabinose isomerase and ribulokinase, were characterized. Further, d -ribose degradation in Halorhabdus species involves ribokinase, ribose-5-phosphate isomerase and d -ribulose-5-phosphate-3-epimerase. Ribokinase of Halorhabdus tiamatea and ribose-5-phosphate isomerase of Halorhabdus utahensis were characterized. This is the first report of pentose degradation via the bacterial-type pathways in archaea, in Halorhabdus species that likely acquired these pathways from bacteria. The utilization of bacterial-type pathways of pentose degradation rather than the archaeal oxidative pathways generating α-ketoglutarate might be explained by an incomplete gluconeogenesis in Halorhabdus species preventing the utilization of α-ketoglutarate in the anabolism.

A bioluminescent reporter for the halophilic archaeon Haloferax volcanii - Chris R. Davis; Carl H. Johnson; J. Brian Robertson (Extremophiles, 2020)

New insights into halophilic prokaryotes isolated from salting–ripening anchovies (Engraulis anchoita) process focused on histamine-degrading strains - Silvina Perez; Silvia Elena Murialdo; Irene Mabel Ameztoy; Noemí Elisabet Zaritzky; María Isabel Yeannes (Extremophiles, 2020)

Metagenomic insights into the fungal assemblages of the northwest Himalayan cold desert - Puja Gupta; Jyoti Vakhlu; Yash Pal Sharma; Madangchanok Imchen; Ranjith Kumavath (Extremophiles, 2020)

Root endophytic Penicillium promotes growth of Antarctic vascular plants by enhancing nitrogen mineralization - Rómulo Oses-Pedraza; Cristian Torres-Díaz; Paris Lavín; Patricio Retamales-Molina; Cristian Atala; Jorge Gallardo-Cerda; Ian S. Acuña-Rodríguez; Marco A. Molina-Montenegro (Extremophiles, 2020)

Boosting Escherichia coli’s heterologous production rate of ectoines by exploiting the non-halophilic gene cluster from Acidiphilium cryptum - Lukas Bethlehem; Katharina D. Moritz (Extremophiles, 2020)

Biochemical characterization of an alkaline surfactant-stable keratinase from a new keratinase producer, Bacillus zhangzhouensis - Roghyeh Moridshahi; Masoumeh Bahreini; Mohammadreza Sharifmoghaddam; Ahmad Asoodeh (Extremophiles, 2020)

Fast decolorization of azo dyes in alkaline solutions by a thermostable metal-tolerant bacterial laccase and proposed degradation pathways - Laura E. Navas; Romina Carballo; Laura Levin; Marcelo F. Berretta (Extremophiles, 2020)

Defining heat shock response for the thermoacidophilic model crenarchaeon Sulfolobus acidocaldarius - Rani Baes; Liesbeth Lemmens; Kim Mignon; Matthias Carlier; Eveline Peeters (Extremophiles, 2020)

Richness and metallo-tolerance of cultivable fungi recovered from three high altitude glaciers from Citlaltépetl and Iztaccíhuatl volcanoes (Mexico) - Rosa Paulina Calvillo-Medina; Nina Gunde-Cimerman; Efraín Escudero-Leyva; Luis Barba-Escoto; Elisa Irma Fernández-Tellez; Ariadna Andrea Medina-Tellez; Victor Bautista-de Lucio; Miguel Ángel Ramos-López; Juan Campos-Guillén (Extremophiles, 2020)

Diversity, ecology, and bioprospecting of culturable fungi in lakes impacted by anthropogenic activities in Maritime Antarctica - Mayara B. Ogaki; Rosemary Vieira; Marcelo C. Muniz; Carlos L. Zani; Tânia M. A. Alves; Policarpo A. S. Junior; Silvane M. F. Murta; Emerson C. Barbosa; Jaquelline G. Oliveira; Isabela P. Ceravolo; Patrícia O. Pereira; Carlos A. Rosa; et al. (Extremophiles, 2020)

Methanogenesis in the Lake Elton saline aquatic system - Anna Kallistova; Alexander Merkel; Timur Kanapatskiy; Yulia Boltyanskaya; Ivan Tarnovetskii; Anna Perevalova; Vadim Kevbrin; Olga Samylina; Nikolai Pimenov (Extremophiles, 2020)

The insight into diatom diversity, ecology, and biogeography of an extreme cold ultraoligotrophic Lake Labynkyr at the Pole of Cold in the northern hemisphere - Liubov Kopyrina; Alena Firsova; Elena Rodionova; Yulia Zakharova; Maria Bashenkhaeva; Marina Usoltseva; Yelena Likhoshway (Extremophiles, 2020)

Improved Isolation of SlaA and SlaB S-layer proteins in Sulfolobus acidocaldarius - Pierre Simonin; Carine Lombard; Arnaud Huguet; Adrienne Kish (Extremophiles, 2020)

Pigment production by cold-adapted bacteria and fungi: colorful tale of cryosphere with wide range applications - Wasim Sajjad; Ghufranud Din; Muhammad Rafiq; Awais Iqbal; Suliman Khan; Sahib Zada; Barkat Ali; Shichang Kang (Extremophiles, 2020)

Salt-tolerant Acidihalobacter and Acidithiobacillus species from Vulcano (Italy) and Milos (Greece) - Paul R. Norris; Carol S. Davis-Belmar; Leonides A. Calvo-Bado; Thomas J. Ogden (Extremophiles, 2020)

Biofilm and planktonic microbial communities in highly acidic soil (pH < 3) in the Soos National Nature Reserve, Czech Republic - Martina Hujslová; Hana Gryndlerová; Lukáš Bystrianský; Hana Hršelová; Milan Gryndler (Extremophiles, 2020)

Effects of pH and temperature on (S)-amine activity of transaminase from the cold-adapted bacterium Psychrobacter cryohalolentis - Ekaterina Yu. Bezsudnova; Tatiana N. Stekhanova; Aleksandr O. Ruzhitskiy; Vladimir O. Popov (Extremophiles, 2020)

Modification of the glycolytic pathway in Pyrococcus furiosus and the implications for metabolic engineering - Christopher T. Straub; Gerritt Schut; Jonathan K. Otten; Lisa M. Keller; Michael W. W. Adams; Robert M. Kelly (Extremophiles, 2020)

Diversity, distribution, and ecology of viable fungi in permafrost and active layer of Maritime Antarctica - Thamar Holanda da Silva; Débora Amorim Saraiva Silva; Fábio Soares de Oliveira; Carlos Ernesto Gonçalves Reynaud Schaefer; Carlos Augusto Rosa; Luiz Henrique Rosa (Extremophiles, 2020)

Geothermal springs in Armenia and Nagorno-Karabakh: potential sources of hydrolase-producing thermophilic bacilli - Hovik Panosyan; Armine Margaryan; Nils-Kåre Birkeland (Extremophiles, 2020)

Carbon monoxide-dependent transcriptional changes in a thermophilic, carbon monoxide-utilizing, hydrogen-evolving bacterium Calderihabitans maritimus KKC1 revealed by transcriptomic analysis - Masao Inoue; Hikaru Izumihara; Yuto Fukuyama; Kimiho Omae; Takashi Yoshida; Yoshihiko Sako (Extremophiles, 2020)
Calderihabitans maritimus KKC1 is a thermophilic, carbon monoxide (CO)-utilizing, hydrogen-evolving bacterium that harbors seven cooS genes for anaerobic CO dehydrogenases and six hyd genes for [NiFe] hydrogenases and capable of using a variety of electron acceptors coupled to CO oxidation. To understand the relationships among these unique features and the transcriptional adaptation of the organism to CO, we performed a transcriptome analysis of C. maritimus KKC1 grown under 100% CO and N 2 conditions. Of its 3114 genes, 58 and 32 genes were significantly upregulated and downregulated in the presence of CO, respectively. A cooS–ech gene cluster, an “orphan” cooS gene, and bidirectional hyd genes were upregulated under CO, whereas hydrogen-uptake hyd genes were downregulated. Transcriptional changes in anaerobic respiratory genes supported the broad usage of electron acceptors in C. maritimus KKC1 under CO metabolism. Overall, the majority of the differentially expressed genes were oxidoreductase-like genes, suggesting metabolic adaptation to the cellular redox change upon CO oxidation. Moreover, our results suggest a transcriptional response mechanism to CO that involves multiple transcription factors, as well as a CO-responsive transcriptional activator (CooA). Our findings shed light on the diverse mechanisms for transcriptional and metabolic adaptations to CO in CO-utilizing and hydrogen-evolving bacteria.

Biochemical comparison of two glucose 6-phosphate dehydrogenase isozymes from a cold-adapted Pseudomonas mandelii - Quynh DangThu; Sei-Heon Jang; ChangWoo Lee (Extremophiles, 2020)

Insights into the metabolism pathway and functional genes of long-chain aliphatic alkane degradation in haloarchaea - Sumit Kumar; Jian Zhou; Ming Li; Hua Xiang; Dahe Zhao (Extremophiles, 2020)

Characteristics of oxidative stress and antioxidant defenses by a mixed culture of acidophilic bacteria in response to Co2+ exposure - Weijin Wu; Xiyan Li; Xu Zhang; Tingyue Gu; Yongqiu Qiu; Minglong Zhu; Wensong Tan (Extremophiles, 2020)

Essentiality of the glnA gene in Haloferax mediterranei: gene conversion and transcriptional analysis - V. Rodríguez-Herrero; G. Payá; V. Bautista; A. Vegara; M. Cortés-Molina; M. Camacho; J. Esclapez; M. J. Bonete (Extremophiles, 2020)

Correction to: Microbial synergy and stoichiometry in heap biooxidation of low-grade porphyry arsenic-bearing gold ore - Jiafeng Li; Linlin Tong; Yu Xia; Hongying Yang; Wolfgang Sand; Hongzhen Xie; Bibo Lan; Shuiping Zhong; Ali Auwalu (Extremophiles, 2020)

Contribution of mechanosensitive channels to osmoadaptation and ectoine excretion in Halomonas elongata - Jasmina Vandrich; Friedhelm Pfeiffer; Gabriela Alfaro-Espinoza; Hans Jörg Kunte (Extremophiles, 2020)
For osmoadaptation the halophilic bacterium Halomonas elongata synthesizes as its main compatible solute the aspartate derivative ectoine. H. elongata does not rely entirely on synthesis but can accumulate ectoine by uptake from the surrounding environment with the help of the osmoregulated transporter TeaABC. Disruption of the TeaABC-mediated ectoine uptake creates a strain that is constantly losing ectoine to the medium. However, the efflux mechanism of ectoine in H. elongata is not yet understood. H. elongata possesses four genes encoding mechanosensitive channels all of which belong to the small conductance type (MscS). Analysis by qRT-PCR revealed a reduction in transcription of the mscS genes with increasing salinity. The response of H. elongata to hypo- and hyperosmotic shock never resulted in up-regulation but rather in down-regulation of mscS transcription. Deletion of all four mscS genes created a mutant that was unable to cope with hypoosmotic shock. However, the knockout mutant grew significantly faster than the wildtype at high salinity of 2 M NaCl, and most importantly, still exported 80% of the ectoine compared to the wildtype. We thus conclude that a yet unknown system, which is independent of mechanosensitive channels, is the major export route for ectoine in H. elongata .

The influence of the specific growth rate on the lipid composition of Sulfolobus acidocaldarius - Julian Quehenberger; Ernst Pittenauer; Günter Allmaier; Oliver Spadiut (Extremophiles, 2020)
Archaeal lipids are constituted of two isoprenoid chains connected via ether bonds to glycerol in the sn -2, 3 position. Due to these unique properties archaeal lipids are significantly more stable against high temperature, low pH, oxidation and enzymatic degradation than conventional lipids. Additionally, in members of the phylum Crenarchaeota condensation of two (monopolar) archaeal diether lipids to a single (bipolar) tetraether lipid as well as formation of cyclopentane rings in the isoprenoid core strongly reduce permeability of the crenarchaeal membranes. In this work we show that the Crenarchaeum Sulfolobus acidocaldarius changes its lipid composition as reaction to a shift in growth rate caused by nutrient limitation. We thereby identified a novel influencing factor for the lipid composition of S. acidocaldarius and were able to determine the effect of this factor on the lipid composition by using MALDI-MS for the semi-quantification of an archaeal lipidome: a shift in the specific growth rate during a controlled continuous cultivation of S. acidocaldarius from 0.011 to 0.035 h −1 led to a change in the ratio of diether to tetraether lipids from 1:3 to 1:5 and a decrease of the average number of cyclopentane rings from 5.1 to 4.6.

Characterization of a novel Cu-containing dissimilatory nitrite reductase from the haloarchaeon Halorussus sp. YCN54 - Jing Hou; Xiao-Yan Yang; Qin Xu; Heng-Lin Cui (Extremophiles, 2020)

Fungi in glacial ice of Antarctica: diversity, distribution and bioprospecting of bioactive compounds - Graciéle Cunha Alves de Menezes; Bárbara Alves Porto; Soraya Sander Amorim; Carlos Leomar Zani; Tânia Maria de Almeida Alves; Policarpo Ademar Sales Junior; Silvane Maria Fonseca Murta; Jefferson Cardia Simões; Betania Barros Cota; Carlos Augusto Rosa; Luiz Henrique Rosa (Extremophiles, 2020)

The evolutionarily conserved HtrA is associated with stress tolerance and protein homeostasis in the halotolerant cyanobacterium Halothece sp. PCC7418 - Tanutcha Patipong; Takashi Hibino; Hakuto Kageyama; Rungaroon Waditee-Sirisattha (Extremophiles, 2020)

Osmolytes and membrane lipids in adaptive response of thermophilic fungus Rhizomucor miehei to cold, osmotic and oxidative shocks - Elena A. Ianutsevich; Olga A. Danilova; Dmitrii V. Kurilov; Igor V. Zavarzin; Vera M. Tereshina (Extremophiles, 2020)

Integration of large heterologous DNA fragments into the genome of Thermococcus kodakarensis - Takaaki Sato; Daisuke Takada; Takashi Itoh; Moriya Ohkuma; Haruyuki Atomi (Extremophiles, 2020)

Microbial synergy and stoichiometry in heap biooxidation of low-grade porphyry arsenic-bearing gold ore - Jiafeng Li; Linlin Tong; Yu Xia; Hongying Yang; Wolfgang Sand; Hongzhen Xie; Bibo Lan; Shuiping Zhong; Ali Auwalu (Extremophiles, 2020)

Volatile organic compounds in the salt-lake sediments of the Tibet Plateau influence prokaryotic diversity and community assembly - Xiaowei Ding; Kaihui Liu; Guoli Gong; Lu Tian; Jun Ma (Extremophiles, 2020)

In Memoriam: Milton S. da Costa (1948–2020) a passion for the beauty of eccentric microbes - Helena Santos (Extremophiles, 2020)

Acidithiobacillus ferrianus sp. nov.: an ancestral extremely acidophilic and facultatively anaerobic chemolithoautotroph - Paul R. Norris; Carmen Falagán; Ana Moya-Beltrán; Matías Castro; Raquel Quatrini; D. Barrie Johnson (Extremophiles, 2020)
Strain MG, isolated from an acidic pond sediment on the island of Milos (Greece), is proposed as a novel species of ferrous iron- and sulfur-oxidizing Acidithiobacillus . Currently, four of the eight validated species of this genus oxidize ferrous iron, and strain MG shares many key characteristics with these four, including the capacities for catalyzing the oxidative dissolution of pyrite and for anaerobic growth via ferric iron respiration. Strain MG also grows aerobically on hydrogen and anaerobically on hydrogen coupled to ferric iron reduction. While the 16S rRNA genes of the iron-oxidizing Acidi-thiobacillus species (and strain MG) are located in a distinct phylogenetic clade and are closely related (98–99% 16S rRNA gene identity), genomic relatedness indexes (ANI/dDDH) revealed strong genomic divergence between strain MG and all sequenced type strains of the taxon, and placed MG as the first cultured representative of an ancestral phylotype of iron oxidizing acidithiobacilli. Strain MG is proposed as a novel species, Acidithiobacillus ferrianus sp. nov. The type strain is MG T (= DSM 107098 T = JCM 33084 T ). Similar strains have been found as isolates or indicated by cloned 16S rRNA genes from several mineral sulfide mine sites.

Consensus protein engineering on the thermostable histone-like bacterial protein HUs significantly improves stability and DNA binding affinity - Anastasios Georgoulis; Maria Louka; Stratos Mylonas; Philemon Stavros; George Nounesis; Constantinos E. Vorgias (Extremophiles, 2020)

Biochemical and phylogenetic characterization of a monomeric isocitrate dehydrogenase from a marine methanogenic archaeon Methanococcoides methylutens - Peng Wang; Yuan Wang; Xiuxiu Guo; Shiping Huang; Guoping Zhu (Extremophiles, 2020)

Statistical optimisation of growth conditions and diesel degradation by the Antarctic bacterium, Rhodococcus sp. strain AQ5‒07 - Ahmad Fareez Ahmad Roslee; Nur Nadhirah Zakaria; Peter Convey; Azham Zulkharnain; Gillian Li Yin Lee; Claudio Gomez-Fuentes; Siti Aqlima Ahmad (Extremophiles, 2020)

Azo dyes decolorization under high alkalinity and salinity conditions by Halomonas sp. in batch and packed bed reactor - B. Montañez-Barragán; J. L. Sanz-Martín; P. Gutiérrez-Macías; A. Morato-Cerro; R. Rodríguez-Vázquez; B. E. Barragán-Huerta (Extremophiles, 2020)

Response to lethal UVA radiation in the Antarctic bacterium Pseudomonas extremaustralis: polyhydroxybutyrate and cold adaptation as protective factors - Paula M. Tribelli; Magdalena Pezzoni; María Gabriela Brito; Nahuel V. Montesinos; Cristina S. Costa; Nancy I. López (Extremophiles, 2020)

Extreme environments: a source of biosurfactants for biotechnological applications - Júnia Schultz; Alexandre Soares Rosado (Extremophiles, 2020)

Comparative genomics analysis of Nitriliruptoria reveals the genomic differences and salt adaptation strategies - Dai-Di Chen; Ye Tian; Jian-Yu Jiao; Xiao-Tong Zhang; Yong-Guang Zhang; Zhou-Yan Dong; Meng-Jie Xiong; Min Xiao; Wen-Sheng Shu; Wen-Jun Li (Extremophiles, 2020)

Cultivable fungi present in deep-sea sediments of Antarctica: taxonomy, diversity, and bioprospecting of bioactive compounds - Mayara B. Ogaki; Lívia C. Coelho; Rosemary Vieira; Arthur A. Neto; Carlos L. Zani; Tânia M. A. Alves; Policarpo A. S. Junior; Silvane M. F. Murta; Emerson C. Barbosa; Jaquelline G. Oliveira; Isabela P. Ceravolo; Patrícia O. Pereira; et al. (Extremophiles, 2020)

A new GH13 subfamily represented by the α-amylase from the halophilic archaeon Haloarcula hispanica - Štefan Janeček; Barbora Zámocká (Extremophiles, 2020)

Diversity and ecology of cultivable fungi isolated from the thermal soil gradients in Deception Island, Antarctica - Hebert M. Figueredo; Vívian N. Gonçalves; Valéria M. Godinho; Daví V. Lopes; Fabio S. Oliveira; Luiz H. Rosa (Extremophiles, 2020)

Extremophiles: a special or general case in the search for extra-terrestrial life? - Ian von Hegner (Extremophiles, 2020)

Pelomicrobium methylotrophicum gen. nov., sp. nov. a moderately thermophilic, facultatively anaerobic, lithoautotrophic and methylotrophic bacterium isolated from a terrestrial mud volcano - G. B. Slobodkina; A. Y. Merkel; A. A. Novikov; E. A. Bonch-Osmolovskaya; A. I. Slobodkin (Extremophiles, 2020)

A plasmid vector that directs hyperproduction of recombinant proteins in the thermophiles Geobacillus species - Ryota Kurashiki; Tatsuki Mizuno; Kurumi Murata; Takashi Ohshiro; Hirokazu Suzuki (Extremophiles, 2020)

Bioremediation potential of a halophilic Halobacillus sp. strain, EG1HP4QL: exopolysaccharide production, crude oil degradation, and heavy metal tolerance - Ibrahim M. Ibrahim; Svetlana A. Konnova; Elena N. Sigida; Elena V. Lyubun; Anna Yu. Muratova; Yulia P. Fedonenko; Кhaled Elbanna (Extremophiles, 2020)

Water masses influence bacterioplankton community structure in summer Kongsfjorden - Shunan Cao; Fang Zhang; Jianfeng He; Zhongqiang Ji; Qiming Zhou (Extremophiles, 2020)

Cell membrane fatty acid and pigment composition of the psychrotolerant cyanobacterium Nodularia spumigena CHS1 isolated from Hopar glacier, Pakistan - Noor Hassan; Alexandre M. Anesio; Muhammad Rafiq; Jens Holtvoeth; Ian Bull; Christopher J. Williamson; Fariha Hasan (Extremophiles, 2020)

Branched-chain amino acid catabolism of Thermoanaerobacter pseudoethanolicus reveals potential route to branched-chain alcohol formation - Sean Michael Scully; Johann Orlygsson (Extremophiles, 2020)

Prokaryotic community in Pleistocene ice wedges of Mammoth Mountain - Andrey Rakitin; Aleksey Beletsky; Andrey Mardanov; Natalya Surgucheva; Vladimir Sorokin; Mariya Cherbunina; Anatoli Brouchkov; Andrey Mulyukin; Svetlana Filippova (Extremophiles, 2020)

A journey down to hell: new thermostable protein-tags for biotechnology at high temperatures - Rosanna Mattossovich; Rosa Merlo; Angelo Fontana; Giuliana d’Ippolito; Michael P. Terns; Elizabeth A. Watts; Anna Valenti; Giuseppe Perugino (Extremophiles, 2020)

Diverse respiratory capacity among Thermus strains from US Great Basin hot springs - En-Min Zhou; Arinola L. Adegboruwa; Chrisabelle C. Mefferd; Shrikant S. Bhute; Senthil K. Murugapiran; Jeremy A. Dodsworth; Scott C. Thomas; Amanda J. Bengtson; Lan Liu; Wen-Dong Xian; Wen-Jun Li; Brian P. Hedlund (Extremophiles, 2020)

Extremophilic models for astrobiology: haloarchaeal survival strategies and pigments for remote sensing - Shiladitya DasSarma; Priya DasSarma; Victoria J. Laye; Edward W. Schwieterman (Extremophiles, 2020)

Microbiomics of Namib Desert habitats - D. A. Cowan; D. W. Hopkins; B. E. Jones; G. Maggs-Kölling; R. Majewska; J.-B. Ramond (Extremophiles, 2020)

The biology and biotechnology of the genus Caldicellulosiruptor: recent developments in ‘Caldi World’ - Laura L. Lee; James R. Crosby; Gabriel M. Rubinstein; Tunyaboon Laemthong; Ryan G. Bing; Christopher T. Straub; Michael W.W. Adams; Robert M. Kelly (Extremophiles, 2020)

Isolation and characterization of violacein from an Antarctic Iodobacter: a non-pathogenic psychrotolerant microorganism - Joaquin Atalah; Lotsé Blamey; Sebastian Muñoz-Ibacache; Felipe Gutierrez; Marcela Urzua; Maria Victoria Encinas; Maritza Páez; Junsong Sun; Jenny M. Blamey (Extremophiles, 2020)

Cell-wall associated polysaccharide from the psychrotolerant bacterium Psychrobacter arcticus 273-4: isolation, purification and structural elucidation - Angela Casillo; Annarita Ricciardelli; Ermenegilda Parrilli; Maria Luisa Tutino; Maria Michela Corsaro (Extremophiles, 2020)

Characterization of thiosulfate reductase from Pyrobaculum aerophilum heterologously produced in Pyrococcus furiosus - Dominik K. Haja; Chang-Hao Wu; Farris L. Poole; John Sugar; Samuel G. Williams; Anne K. Jones; Michael W. W. Adams (Extremophiles, 2020)

A meta-taxonomic investigation of the prokaryotic diversity of water bodies impacted by acid mine drainage from the São Domingos mine in southern Portugal - Sara Ettamimi; Jorge D. Carlier; Cymon J. Cox; Youssef Elamine; Khalil Hammani; Hassan Ghazal; Maria C. Costa (Extremophiles, 2019)

Effects of the substituted amino acid residues on the thermal properties of monomeric isocitrate dehydrogenases from a psychrophilic bacterium, Psychromonas marina, and a mesophilic bacterium, Azotobacter vinelandii - Kango Tsubouchi; Yasuhiro Takada (Extremophiles, 2019)

Proto-dolomite formation in microbial consortia dominated by Halomonas strains - Ammar Alibrahim; Dunia Al-Gharabally; Huda Mahmoud; Maria Dittrich (Extremophiles, 2019)

Colombian Andean thermal springs: reservoir of thermophilic anaerobic bacteria producing hydrolytic enzymes - Carolina Rubiano-Labrador; Carolina Díaz-Cárdenas; Gina López; Javier Gómez; Sandra Baena (Extremophiles, 2019)

Purification and characterization of a novel thermophilic β-galactosidase from Picrophilus torridus of potential industrial application - Jayne Murphy; Gary Walsh (Extremophiles, 2019)

Two novel alkaliphiles, Bacillus alkalisoli sp. nov., and Bacillus solitudinis sp. nov., isolated from saline-alkali soil - Guo-Hong Liu; Manik Prabhu Narsing Rao; Zhou-Yan Dong; Jie-Ping Wang; Zheng Chen; Bo Liu; Wen-Jun Li (Extremophiles, 2019)

Diversity and functional profile of bacterial communities at Lancaster acid mine drainage dam, South Africa as revealed by 16S rRNA gene high-throughput sequencing analysis - Thabile Lukhele; Ramganesh Selvarajan; Hlengilizwe Nyoni; Bheki Brilliance Mamba; Titus Alfred Makudali Msagati (Extremophiles, 2019)

Cultivation technology development of Rhodothermus marinus DSM 16675 - Emanuel Y. C. Ron; Roya R. R. Sardari; Richard Anthony; Ed W. J. van Niel; Gudmundur O. Hreggvidsson; Eva Nordberg-Karlsson (Extremophiles, 2019)
This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LB malt ) resulted in higher cell density (OD 620 = 6.6) than use of MB malt (OD 620 = 1.7). Sequential batch cultivation increased the cell density threefold (OD 620 = 20) in LB malt and eightfold (OD 620 = 14) in MB malt . In both single and sequential batches, the production of carotenoids and EPSs using LB malt was detected in the exponential phase and stationary phase, respectively, while in MB malt formation of both products was detectable in both the exponential and stationary phases of the culture. Heteropolymeric EPSs were produced with an overall volumetric productivity ( Q E ) of 0.67 (mg/L h) in MB malt and the polymer contained xylose. In LB, Q E was lower (0.1 mg/L h) and xylose could not be detected in the composition of the produced EPSs. In conclusion, this study showed the importance of a process design and medium source for production of R. marinus DSM 16675 and its metabolites.

Fischerella thermalis: a model organism to study thermophilic diazotrophy, photosynthesis and multicellularity in cyanobacteria - Jaime Alcorta; Pablo Vergara-Barros; Laura A. Antonaru; María E. Alcamán-Arias; Dennis J. Nürnberg; Beatriz Díez (Extremophiles, 2019)

Comparative evaluation of three archaeal primer pairs for exploring archaeal communities in deep-sea sediments and permafrost soils - Shiping Wei; Hongpeng Cui; Yuchen Zhang; Xin Su; Hailiang Dong; Fang Chen; Youhai Zhu (Extremophiles, 2019)

Identification of a novel protease from the thermophilic Anoxybacillus kamchatkensis M1V and its application as laundry detergent additive - Sondes Mechri; Khelifa Bouacem; Nadia Zaraî Jaouadi; Hatem Rekik; Mouna Ben Elhoul; Maroua Omrane Benmrad; Hocine Hacene; Samir Bejar; Amel Bouanane-Darenfed; Bassem Jaouadi (Extremophiles, 2019)

A highly stable manganese catalase from Geobacillus thermopakistaniensis: molecular cloning and characterization - Abeera Shaeer; Mehwish Aslam; Naeem Rashid (Extremophiles, 2019)

Characterization and homology modelling of a novel multi-modular and multi-functional Paenibacillus mucilaginosus glycoside hydrolase - Ntsoaki Leticia Mosina; Wolf-Dieter Schubert; Don A. Cowan (Extremophiles, 2019)

Crystal structures of CDC21-1 inteins from hyperthermophilic archaea reveal the selection mechanism for the highly conserved homing endonuclease insertion site - Hannes M. Beyer; Kornelia M. Mikula; Tatiana V. Kudling; Hideo Iwaï (Extremophiles, 2019)

A thermostable 5-enolpyruvylshikimate-3-phosphate synthase from Thermotoga maritima enhances glyphosate tolerance in Escherichia coli and transgenic Arabidopsis - Lijuan Wang; Rihe Peng; Yongsheng Tian; Jianjie Gao; Bo Wang; Quanhong Yao (Extremophiles, 2019)

Distinct roles of an ionic interaction holding an alpha-helix with catalytic Asp and a beta-strand with catalytic His in a hyperthermophilic esterase EstE1 and a mesophilic esterase rPPE - VinayKumar Dachuri; Ngoc Truongvan; Quynh DangThu; Sei-Heon Jang; ChangWoo Lee (Extremophiles, 2019)

Endonucleases responsible for DNA repair of helix-distorting DNA lesions in the thermophilic crenarchaeon Sulfolobus acidocaldarius in vivo - Shoji Suzuki; Norio Kurosawa (Extremophiles, 2019)

A novel thermophilic Aeribacillus bacteriophage AP45 isolated from the Valley of Geysers, Kamchatka: genome analysis suggests the existence of a new genus within the Siphoviridae family - V. Morozova; O. Bokovaya; Yu. Kozlova; A. Kurilshikov; I. Babkin; A. Tupikin; A. Bondar; E. Ryabchikova; A. Brayanskaya; S. Peltek; N. Tikunova (Extremophiles, 2019)

Correction to: Aspergillus loretoensis, a single isolate from marine sediment of Loreto Bay, Baja California Sur, México resulting as a new obligate halophile species - Sophia González-Martínez; Clara Galindo-Sánchez; Edgar López-Landavery; Carmen Paniagua-Chávez; Amelia Portillo-López (Extremophiles, 2019)

Reference genes for real-time RT-PCR expression studies in an Antarctic Pseudomonas exposed to different temperature conditions - César X. García-Laviña; Susana Castro-Sowinski; Ana Ramón (Extremophiles, 2019)

Oxidative stress under low oxygen conditions triggers hyperflagellation and motility in the Antarctic bacterium Pseudomonas extremaustralis - Esmeralda C. Solar Venero; Martiniano M. Ricardi; María Gomez-Lozano; Søren Molin; Paula M. Tribelli; Nancy I. López (Extremophiles, 2019)

Purification and biochemical characterization of a novel thermostable and halotolerant subtilisin SAPN, a serine protease from Melghiribacillus thermohalophilus Nari2AT for chitin extraction from crab and shrimp shell by-products - Sondes Mechri; Khelifa Bouacem; Fadoua Jabeur; Sara Mohamed; Nariman Ammara Addou; Ahlam Dab; Aicha Bouraoui; Amel Bouanane-Darenfed; Samir Bejar; Hocine Hacène; Laura Baciou; Florence Lederer; et al. (Extremophiles, 2019)

Pleurastrosarcina terriformae, a new species of a rare desert trebouxiophycean alga discovered by an integrative approach - Tatyana Darienko; Woojean Kang; Aleksander K. Orzechowski; Thomas Pröschold (Extremophiles, 2019)

Aspergillus loretoensis, a single isolate from marine sediment of Loreto Bay, Baja California Sur, México resulting as a new obligate halophile species - Sophia González-Martínez; Clara Galindo-Sánchez; Edgar López-Landavery; Carmen Paniagua-Chávez; Amelia Portillo-López (Extremophiles, 2019)

Production of highly catalytic, archaeal Pd(0) bionanoparticles using Sulfolobus tokodaii - Santisak Kitjanukit; Keiko Sasaki; Naoko Okibe (Extremophiles, 2019)

Recombinant production and characterization of a novel esterase from a hypersaline lake, Acıgöl, by metagenomic approach - Havva Esra Tutuncu; Nurgul Balci; Melek Tuter; Nevin Gul Karaguler (Extremophiles, 2019)

Structural determinants increasing flexibility confer cold adaptation in psychrophilic phosphoglycerate kinase - David Mandelman; Lionel Ballut; David A. Wolff; Georges Feller; Charles Gerday; Richard Haser; Nushin Aghajari (Extremophiles, 2019)

Description of Halegenticoccus soli gen. nov., sp. nov., a halophilic archaeon isolated from a soil sample of Ebi lake - Bing-Bing Liu; Manik Prabhu Narsing Rao; Xiao-Qing Yin; Xin Li; Nimaichand Salam; Yao Zhang; Dalal Hussien M. Alkhalifah; Wael N. Hozzein; Wen-Jun Li (Extremophiles, 2019)

Eukaryotic organisms of continental hydrothermal systems - Sabrina R. Brown; Sherilyn C. Fritz (Extremophiles, 2019)

Characterization of a thermoactive endoglucanase isolated from a biogas plant metagenome - Barbara Klippel; Saskia Blank; Viktoria-Astrid Janzer; Henning Piascheck; Cyril Moccand; Rachid Bel-Rhlid; Garabed Antranikian (Extremophiles, 2019)

Toward a rapid method for the study of biodiversity in cold environments: the characterization of psychrophilic yeasts by MALDI-TOF mass spectrometry - Joseph J. Dalluge; Elizabeth C. Brown; Laurie B. Connell (Extremophiles, 2019)

Dual bloom of green algae and purple bacteria in an extremely shallow soda pan - Kristóf Korponai; Attila Szabó; Boglárka Somogyi; Emil Boros; Andrea K. Borsodi; Laura Jurecska; Lajos Vörös; Tamás Felföldi (Extremophiles, 2019)

Lipids and soluble carbohydrates in the mycelium and ascomata of alkaliphilic fungus Sodiomyces alkalinus - Maria V. Kozlova; Elena A. Ianutsevich; Olga A. Danilova; Olga V. Kamzolkina; Vera M. Tereshina (Extremophiles, 2019)

Production, characterization and antimicrobial activities of bio-pigments by Aquisalibacillus elongatus MB592, Salinicoccus sesuvii MB597, and Halomonas aquamarina MB598 isolated from Khewra Salt Range, Pakistan - Anila Fariq; Azra Yasmin; Muhammad Jamil (Extremophiles, 2019)

Identification of a GntR family regulator BusRTha and its regulatory mechanism in the glycine betaine ABC transport system of Tetragenococcus halophilus - Jieting Lin; Yunfei Zhu; Hanlan Tang; Junwei Yan; Lixin Luo (Extremophiles, 2019)

Identification of a novel esterase from the thermophilic bacterium Geobacillus thermodenitrificans NG80-2 - Nicola Curci; Andrea Strazzulli; Federica De Lise; Roberta Iacono; Luisa Maurelli; Fabrizio Dal Piaz; Beatrice Cobucci-Ponzano; Marco Moracci (Extremophiles, 2019)

Exploring the piezotolerant/piezophilic microbial community and genomic basis of piezotolerance within the deep subsurface Deccan traps - Avishek Dutta; Logan M. Peoples; Abhishek Gupta; Douglas H. Bartlett; Pinaki Sar (Extremophiles, 2019)

High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals - Lidija Izrael-Živković; Vladimir Beškoski; Milena Rikalović; Snježana Kazazić; Nicole Shapiro; Tanja Woyke; Gordana Gojgić-Cvijović; Miroslav M. Vrvić; Nela Maksimović; Ivanka Karadžić (Extremophiles, 2019)

Transcriptome analysis of a thermophilic and hydrogenogenic carboxydotroph Carboxydothermus pertinax - Yuto Fukuyama; Kimiho Omae; Takashi Yoshida; Yoshihiko Sako (Extremophiles, 2019)

Protein acetylation on 2-isopropylmalate synthase from Thermus thermophilus HB27 - Ayako Yoshida; Minoru Yoshida; Tomohisa Kuzuyama; Makoto Nishiyama; Saori Kosono (Extremophiles, 2019)

In Memoriam: Maria Ciaramella (1958–2018) - Giuseppe Perugino; Anna Valenti; Beatrice Cobucci-Ponzano; Marco Moracci; Mosè Rossi (Extremophiles, 2019)

The diversity, distribution, and pathogenic potential of cultivable fungi present in rocks from the South Shetlands archipelago, Maritime Antarctica - Isabel M. S. Alves; Vívian N. Gonçalves; Fabio S. Oliveira; Carlos E. G. R. Schaefer; Carlos A. Rosa; Luiz H. Rosa (Extremophiles, 2019)

Sedimentary Marl mudstone as a substrate in a xeric environment revealed by microbiome analysis - Oksana Koryachenko; Ruben Girsowicz; Yaron Dekel; Tirza Doniger; Yosef Steinberger (Extremophiles, 2019)

Fungi, a neglected component of acidophilic biofilms: do they have a potential for biotechnology? - Martina Hujslová; Lukáš Bystrianský; Oldřich Benada; Milan Gryndler (Extremophiles, 2019)

Temperature adaptation of DNA ligases from psychrophilic organisms - Kristel Berg; Ingar Leiros; Adele Williamson (Extremophiles, 2019)

Labelling halophilic Archaea using 13C and 15N stable isotopes: a potential tool to investigate haloarchaea consumption by metazoans - R. M. A. Lopes-dos-Santos; Marleen De Troch; Peter Bossier; Gilbert Van Stappen (Extremophiles, 2019)

Response of neutrophilic Shewanella violacea to acid stress: growth rate, organic acid production, and gene expression - Lisa Lisdiana; Hisashi Ômura; Sotaro Fujii; Yoshihiro Sambongi (Extremophiles, 2019)

Change in the microbial community of saline geothermal fluids amended with a scaling inhibitor: effects of heat extraction and nitrate dosage - Anke Westphal; Florian Eichinger; Lorenz Eichinger; Hilke Würdemann (Extremophiles, 2019)

Enrichment of anaerobic heterotrophic thermophiles from four Azorean hot springs revealed different community composition and genera abundances using recalcitrant substrates - Marcel Suleiman; Barbara Klippel; Philip Busch; Christian Schäfers; Cyril Moccand; Rachid Bel-Rhlid; Stefan Palzer; Garabed Antranikian (Extremophiles, 2019)

Alkaline soda Lake Velika Rusanda (Serbia): the first insight into diatom diversity of this extreme saline lake - Danijela Vidaković; Jelena Krizmanić; Biljana P. Dojčinović; Ana Pantelić; Bojan Gavrilović; Milica Živanović; Boris Novaković; Miloš Ćirić (Extremophiles, 2019)

The bacterial diversity on steam vents from Paricutín and Sapichu volcanoes - Elcia Margareth Souza Brito; Víctor Manuel Romero-Núñez; César Augusto Caretta; Pierre Bertin; Julio César Valerdi-Negreros; Rémy Guyoneaud; Marisol Goñi-Urriza (Extremophiles, 2019)

Stability of cytochromes c′ from psychrophilic and piezophilic Shewanella species: implications for complex multiple adaptation to low temperature and high hydrostatic pressure - Asako Suka; Hiroya Oki; Yuki Kato; Kazuki Kawahara; Tadayasu Ohkubo; Takahiro Maruno; Yuji Kobayashi; Sotaro Fujii; Satoshi Wakai; Lisa Lisdiana; Yoshihiro Sambongi (Extremophiles, 2019)

A linear pathway for mevalonate production supports growth of Thermococcus kodakarensis - Geraldy L. S. Liman; Tyler Hulko; Hallie P. Febvre; Aaron C. Brachfeld; Thomas J. Santangelo (Extremophiles, 2019)

Physico-chemical approach to adhesion of Alicyclobacillus cells and spores to model solid materials - Jan Strejc; Lucie Kyselova; Anna Cadkova; Tomas Potocar; Tomas Branyik (Extremophiles, 2019)

Microbial community composition and dolomite formation in the hypersaline microbial mats of the Khor Al-Adaid sabkhas, Qatar - Zach A. DiLoreto; Tomaso R. R. Bontognali; Zulfa A. Al Disi; Hamad Al Saad Al-Kuwari; Kenneth H. Williford; Christian J. Strohmenger; Fadhil Sadooni; Christine Palermo; John M. Rivers; Judith A. McKenzie; Michael Tuite; Maria Dittrich (Extremophiles, 2019)

Thermoplasmatales and sulfur-oxidizing bacteria dominate the microbial community at the surface water of a CO2-rich hydrothermal spring located in Tenorio Volcano National Park, Costa Rica - Alejandro Arce-Rodríguez; Fernando Puente-Sánchez; Roberto Avendaño; María Martínez-Cruz; J. Maarten de Moor; Dietmar H. Pieper; Max Chavarría (Extremophiles, 2019)

Genome of the candidate phylum Aminicenantes bacterium from a deep subsurface thermal aquifer revealed its fermentative saccharolytic lifestyle - Vitaly V. Kadnikov; Andrey V. Mardanov; Alexey V. Beletsky; Olga V. Karnachuk; Nikolai V. Ravin (Extremophiles, 2019)

Biogeography of thermophiles and predominance of Thermus scotoductus in domestic water heaters - Regina L. Wilpiszeski; Zhidan Zhang; Christopher H. House (Extremophiles, 2019)

Elucidating functions of DP1 and DP2 subunits from the Thermococcus kodakarensis family D DNA polymerase - Natsuki Takashima; Sonoko Ishino; Keisuke Oki; Mika Takafuji; Takeshi Yamagami; Ryotaro Matsuo; Kouta Mayanagi; Yoshizumi Ishino (Extremophiles, 2019)

Cuniculiplasmataceae, their ecogenomic and metabolic patterns, and interactions with ‘ARMAN’ - Olga V. Golyshina; Rafael Bargiela; Peter N. Golyshin (Extremophiles, 2019)

Taxonomy and richness of yeasts associated with angiosperms, bryophytes, and meltwater biofilms collected in the Antarctic Peninsula - Eskálath Morganna Silva Ferreira; Francisca Maria Pinheiro de Sousa; Luiz Henrique Rosa; Raphael Sanzio Pimenta (Extremophiles, 2019)

Improved production of bacteriorhodopsin from Halobacterium salinarum through direct amino acid supplement in the basal medium - C. Jeganathan; K. Thamaraiselvi; T. C. Sabari Girisun (Extremophiles, 2019)

Increase of positive supercoiling in a hyperthermophilic archaeon after UV irradiation - A. Gorlas; R. Catchpole; E. Marguet; P. Forterre (Extremophiles, 2019)

Combined transcriptomics–metabolomics profiling of the heat shock response in the hyperthermophilic archaeon Pyrococcus furiosus - Ana M. Esteves; Gonçalo Graça; Lindsay Peyriga; Inês M. Torcato; Nuno Borges; Jean-Charles Portais; Helena Santos (Extremophiles, 2019)

In silico and experimental improvement of bacteriorhodopsin production in Halobacterium salinarum R1 by increasing DNA-binding affinity of Bat through Q661R/Q665R substitutions in HTH motif - Azadeh Mirfeizollahi; Bagher Yakhchali; Ali Asghar Deldar; Ali Asghar Karkhane (Extremophiles, 2019)

Behavior of the extremophile green alga Coccomyxa melkonianii SCCA 048 in terms of lipids production and morphology at different pH values - Santina Soru; Veronica Malavasi; Pierluigi Caboni; Alessandro Concas; Giacomo Cao (Extremophiles, 2019)

Acknowledgment to reviewers 2018 (Extremophiles, 2019)

Effect of copper on diesel degradation in Pseudomonas extremaustralis - María Antonela Colonnella; Leonardo Lizarraga; Leticia Rossi; Rocío Díaz Peña; Diego Egoburo; Nancy I. López; Laura J. Raiger Iustman (Extremophiles, 2019)

High diversity of thermophilic cyanobacteria in Rupite hot spring identified by microscopy, cultivation, single-cell PCR and amplicon sequencing - Otakar Strunecký; Karel Kopejtka; Franz Goecke; Jürgen Tomasch; Jaromír Lukavský; Amir Neori; Silke Kahl; Dietmar H. Pieper; Plamen Pilarski; David Kaftan; Michal Koblížek (Extremophiles, 2019)

CRISPR RNA-guided DNA cleavage by reconstituted Type I-A immune effector complexes - Sonali Majumdar; Michael P. Terns (Extremophiles, 2019)

Occurrence of the black yeast Hortaea werneckii in the Mediterranean Sea - Filomena De Leo; Angelina Lo Giudice; Chiara Alaimo; Giusy De Carlo; Alessandro Ciro Rappazzo; Marco Graziano; Emilio De Domenico; Clara Urzì (Extremophiles, 2019)

A highly efficient and cost-effective recombinant production of a bacterial photolyase from the Antarctic isolate Hymenobacter sp. UV11 - Juan José Marizcurrena; Wilner Martínez-López; Hongju Ma; Tilman Lamparter; Susana Castro-Sowinski (Extremophiles, 2019)

Extremely stable indole-3-glycerol-phosphate synthase from hyperthermophilic archaeon Pyrococcus furiosus - Muhammad Arif; Naeem Rashid; Sumera Perveen; Qamar Bashir; Muhammad Akhtar (Extremophiles, 2019)

Thermus sediminis sp. nov., a thiosulfate-oxidizing and arsenate-reducing organism isolated from Little Hot Creek in the Long Valley Caldera, California - En-Min Zhou; Wen-Dong Xian; Chrisabelle C. Mefferd; Scott C. Thomas; Arinola L. Adegboruwa; Nathan Williams; Senthil K. Murugapiran; Jeremy A. Dodsworth; Rakesh Ganji; Meng-Meng Li; Yi-Ping Ding; Lan Liu; et al. (Extremophiles, 2018)

Enzymological characteristics of a novel archaeal dye-linked d-lactate dehydrogenase showing loose binding of FAD - Takenori Satomura; Junji Hayashi; Tatsuya Ohshida; Haruhiko Sakuraba; Toshihisa Ohshima; Shin-ichiro Suye (Extremophiles, 2018)

A metabolic and genomic assessment of sugar fermentation profiles of the thermophilic Thermotogales, Fervidobacterium pennivorans - Scott Wushke; Brian Fristensky; Xiang Li Zhang; Vic Spicer; Oleg V. Krokhin; David B. Levin; Matthew B. Stott; Richard Sparling (Extremophiles, 2018)

Effects of cadmium exposure on expression of glutathione synthetase system genes in Acidithiobacillus ferrooxidans - Chunli Zheng; Li Zhang; Minjie Chen; Xue Qiang Zhao; Yizhuo Duan; Ye Meng; Xuefeng Zhang; Ren Fang Shen (Extremophiles, 2018)

Lipid A structural characterization from the LPS of the Siberian psychro-tolerant Psychrobacter arcticus 273-4 grown at low temperature - Angela Casillo; Marcello Ziaco; Buko Lindner; Ermenegilda Parrilli; Dominik Schwudke; Aurora Holgado; Rudi Beyaert; Rosa Lanzetta; Maria Luisa Tutino; Maria Michela Corsaro (Extremophiles, 2018)

Biogeography and taxonomic overview of terrestrial hot spring thermophilic phages - Olivier Zablocki; Leonardo van Zyl; Marla Trindade (Extremophiles, 2018)

Bacterial and archaeal profiling of hypersaline microbial mats and endoevaporites, under natural conditions and methanogenic microcosm experiments - José Q. García-Maldonado; Alejandra Escobar-Zepeda; Luciana Raggi; Brad M. Bebout; Alejandro Sanchez-Flores; Alejandro López-Cortés (Extremophiles, 2018)

Characterization of protistan plankton diversity in ancient salt evaporation ponds located in a volcanic crater on the island Sal, Cape Verde - Feng Zhao; Sabine Filker (Extremophiles, 2018)

Resistance and Raman spectroscopy analysis of Parageobacillus thermantarcticus spores after γ-ray exposure - Ida Romano; Annalisa De Angelis; Annarita Poli; Pietro Ragni; Laura Lilla; Gianluigi Zito; Barbara Nicolaus; Anna Chiara De Luca; Paola Di Donato (Extremophiles, 2018)

Surviving in hot and cold: psychrophiles and thermophiles from Deception Island volcano, Antarctica - Amanda G. Bendia; Gabriel G. Araujo; André A. Pulschen; Bruna Contro; Rubens T. D. Duarte; Fábio Rodrigues; Douglas Galante; Vivian H. Pellizari (Extremophiles, 2018)

Improvement of the transfucosylation activity of α-l-fucosidase from Thermotoga maritima for the synthesis of fucosylated oligosaccharides in the presence of calcium and sodium - Francisco Guzmán-Rodríguez; Sergio Alatorre-Santamaría; Lorena Gómez-Ruiz; Gabriela Rodríguez-Serrano; Mariano García-Garibay; Alma Cruz-Guerrero (Extremophiles, 2018)

Structural characterization of geranylgeranyl pyrophosphate synthase GACE1337 from the hyperthermophilic archaeon Geoglobus acetivorans - Tatiana E. Petrova; Konstantin M. Boyko; Alena Yu. Nikolaeva; Tatiana N. Stekhanova; Eugeny V. Gruzdev; Andrey V. Mardanov; Viktor S. Stroilov; Jennifer A. Littlechild; Vladimir O. Popov; Ekaterina Yu. Bezsudnova (Extremophiles, 2018)

Potential of Lentibacillus sp. NS12IITR for production of lipids with enriched branched-chain fatty acids for improving biodiesel properties along with hydrocarbon co-production - Noopur Singh; Bijan Choudhury (Extremophiles, 2018)

Bioleaching of copper- and zinc-bearing ore using consortia of indigenous iron-oxidizing bacteria - Wasim Sajjad; Guodong Zheng; Gaosen Zhang; Xiangxian Ma; Wang Xu; Suliman Khan (Extremophiles, 2018)

The complete genome sequence of Rhodobaca barguzinensis alga05 (DSM 19920) documents its adaptation for life in soda lakes - Karel Kopejtka; Jürgen Tomasch; Boyke Bunk; Cathrin Spröer; Irene Wagner-Döbler; Michal Koblížek (Extremophiles, 2018)

Modification of translation factor aIF5A from Sulfolobus solfataricus - F. Bassani; A. Romagnoli; T. Cacciamani; A. Amici; D. Benelli; P. Londei; B. Märtens; U. Bläsi; A. La Teana (Extremophiles, 2018)

Competition of As and other Group 15 elements for surface binding sites of an extremophilic Acidomyces acidophilus isolated from a historical tin mining site - Wai Kit Chan; Dirk Wildeboer; Hemda Garelick; Diane Purchase (Extremophiles, 2018)

Characterization of esterase activity from an Acetomicrobium hydrogeniformans enzyme with high structural stability in extreme conditions - Patricia S. Kumagai; Raissa F. Gutierrez; Jose L. S. Lopes; Julia M. Martins; David M. Jameson; Aline M. Castro; Luiz F. Martins; Ricardo DeMarco; Nelma R. S. Bossolan; B. A. Wallace; Ana P. U. Araujo (Extremophiles, 2018)

Abundance and diversity of prokaryotes in ephemeral hypersaline lake Chott El Jerid using Illumina Miseq sequencing, DGGE and qPCR assays - Manel Ben Abdallah; Fatma Karray; Najwa Kallel; Fabrice Armougom; Najla Mhiri; Marianne Quéméneur; Jean-Luc Cayol; Gaël Erauso; Sami Sayadi (Extremophiles, 2018)