LncRNA USP30-AS1 promotes the survival of acute myeloid leukemia cells by cis-regulating USP30 and ANKRD13A - Wei Zhou; Shilin Xu; Tingfen Deng; Ruiqing Zhou; Caixia Wang (Human Cell, 2022)
Acute myeloid leukemia (AML) is a malignant tumor derived from leukemia stem cells, with complicated pathogenesis. LncRNAs play an important role in tumors genesis and progression. According to results from bioinformatics analysis, lncRNA USP30-AS1 is highly expressed in AML and both the high expression of USP30-AS1 and low methylation level at Cg03124318 locus of USP30-AS1 gene promoter are associated with poor prognosis of AML. This study knocked down and overexpressed USP30-AS1 to determine the roles in AML cell lines. High-throughput sequencing was performed to explore the genes regulated by USP30-AS1. Results showed that USP30-AS1 promoted AML cell viability and inhibited apoptosis. Genes regulated by USP30-AS1 are mainly related to genetic regulation and immune system. Among them, USP30 and ANKRD13A genes are close to USP30-AS1 gene in chromosome. Knockdown of USP30, but not ANKRD13A, abolished the cancer-promoting effects of USP30-AS1. ANKRD13A recognizes Lys-63-linked polyubiquitin chain in HLA-I. USP30-AS1 induced HLA-I internalization from the cell membrane by up-regulating ANKRD13A, which might induce the immune escape of AML cells. ChIP analysis revealed that the regulatory effects of USP30-AS1 on USP30 and ANKRD13A are associated with H3K4me3 and H3K27Ac. In summary, USP30-AS1 probably promotes AML cell survival by cis -regulating USP30 and ANKRD13A.

Liquid biopsies in epilepsy: biomarkers for etiology, diagnosis, prognosis, and therapeutics - Jordan H. Whitlock; Tabea M. Soelter; Avery S. Williams; Andrew A. Hardigan; Brittany N. Lasseigne (Human Cell, 2022)
Epilepsy is one of the most common diseases of the central nervous system, impacting nearly 50 million people around the world. Heterogeneous in nature, epilepsy presents in children and adults alike. Currently, surgery is one treatment approach that can completely cure epilepsy. However, not all individuals are eligible for surgical procedures or have successful outcomes. In addition to surgical approaches, antiepileptic drugs (AEDs) have also allowed individuals with epilepsy to achieve freedom from seizures. Others have found treatment through nonpharmacologic approaches such as vagus nerve stimulation, or responsive neurostimulation. Difficulty in accessing samples of human brain tissue along with advances in sequencing technology have driven researchers to investigate sampling liquid biopsies in blood, serum, plasma, and cerebrospinal fluid within the context of epilepsy. Liquid biopsies provide minimal or non-invasive sample collection approaches and can be assayed relatively easily across multiple time points, unlike tissue-based sampling. Various efforts have investigated circulating nucleic acids from these samples including microRNAs, cell-free DNA, transfer RNAs, and long non-coding RNAs. Here, we review nucleic acid-based liquid biopsies in epilepsy to improve understanding of etiology, diagnosis, prediction, and therapeutic monitoring.

Histone deacetylase 6-mediated downregulation of TMEM100 expedites the development and progression of non-small cell lung cancer - Yanyun Wang; Minwen Ha; Man Li; Lin Zhang; Yitong Chen (Human Cell, 2022)

Ebola viral receptor Niemann–Pick C1 (NPC1) in human cancers: a potential biomarker and immunotherapeutic target - Zhen Fang; Peijuan Li; Liang Shang; Fei Li (Human Cell, 2022)

Establishment and characterization of NCC-UPS3-C1: a novel patient-derived cell line of undifferentiated pleomorphic sarcoma - Ryuto Tsuchiya; Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Takuya Ono; Taro Akiyama; Jun Sugaya; Fumihiko Nakatani; Naoki Kojima; Akihiko Yoshida; Seiji Ohtori; Akira Kawai; et al. (Human Cell, 2022)

Long noncoding RNA XIST modulates microRNA-135/CREB1 axis to influence osteogenic differentiation of osteoblast-like cells in mice with tibial fracture healing - Ying Zhang; Qiang Yuan; Qiushi Wei; Peifeng Li; Zhikun Zhuang; Jitian Li; Youwen Liu; Leilei Zhang; Zhinan Hong; Wei He; Haibin Wang; Wuyin Li (Human Cell, 2022)

Histone methyltransferase DOT1L mediates the TGF-β1/Smad3 signaling pathway through epigenetic modification of SYK in myocardial infarction - Fei Li; Lei Li; Jiacheng Zhang; Xuesong Yang; Yang Liu (Human Cell, 2022)

F-circEA1 regulates cell proliferation and apoptosis through ALK downstream signaling pathway in non-small cell lung cancer - Yinping Huo; Tangfeng Lv; Mingxiang Ye; Suhua Zhu; Jiaxin Liu; Hongbing Liu; Yong Song (Human Cell, 2022)
Studies have confirmed that circular RNA (circRNA) has a stable closed structure, which plays an important role in the progression of tumors. Cancers with positive fusion genes can produce associated fusion circRNA (F-cirRNA). However, there are no reports concerning a role for F-circRNA of the echinoderm microtubule associated-protein like 4-anaplastic lymphoma kinase variant 1 (EML4-ALK1) in non-small cell lung cancer (NSCLC). Our study confirmed the existence of fusion circEA1 (F-circEA1) in NCI-H3122 cells (carrying the EML4-ALK1 gene), F-circEA1 was expressed both in the cytoplasm and nucleus as determined by fluorescence in situ hybridization (FISH) and Sanger sequencing. CCK8 and transwell assays showed that F-circEA1 was beneficial to cell proliferation, metastasis, and invasion. Overexpression of F-circEA1 can also promote cell proliferation, migration and invasion in A549 and SPCA1 cells (non-small cell lung cancer cell line not carrying the EML4-ALK1 gene). Interference with F-circEA1, induced cell cycle arrest and promoted apoptosis as determined by flow cytometry, and increased drug sensitivity to crizotinib in H3122 cells. F-circEA1 directly affected the expression of parental gene EML4-ALK1. Further research found that F-circEA1 can affect the downstream signaling pathway of ALK. In vivo, the growth rate of xenogeneic tumors was reduced and the protein expression level of EML4-ALK1 was significantly decreased in transplanted tumors measured by immunohistochemistry (IHC) after interference with F-circEA1. In conclusion, F-circEA1 can be considered as a proto-oncogene that regulates cell proliferation and apoptosis by affecting the expression of the parental gene EML4-ALK1 and its ALK downstream signaling pathway in non-small cell lung cancer.

Pan-cancer analysis reveals homologous recombination deficiency score as a predictive marker for immunotherapy responders - Can Yang; Zijing Zhang; Xuemei Tang; Xinju Zhang; Yuming Chen; Tingting Hu; Huating Zhang; Ming Guan; Xiuming Zhang; Zhiyuan Wu (Human Cell, 2022)

TRIM59 promotes osteosarcoma progression via activation of STAT3 - Guoxing Xu; Zhenjiang Ma; Fei Yang; Yanqiang Bai; Jian Li; Wanglin Luo; Jiangbo Zhong (Human Cell, 2022)

Are we getting closer to the role of one-carbon metabolism and methylation in the onset of Down syndrome? - Márcia Rodrigues Amorim; Marcelo Aguiar Costa Lima (Human Cell, 2022)

Piezo1 mediates endothelial atherogenic inflammatory responses via regulation of YAP/TAZ activation - Ying Yang; Danyang Wang; Chunxiao Zhang; Wenqing Yang; Chao Li; Zichen Gao; Ke Pei; Yunlun Li (Human Cell, 2022)

Lactate shuttle: from substance exchange to regulatory mechanism - Xingchen Wang; He Liu; Yingqian Ni; Peibo Shen; Xiuzhen Han (Human Cell, 2022)

Macrophage/microglia-derived IL-1β induces glioblastoma growth via the STAT3/NF-κB pathway - Keitaro Kai; Yoshihiro Komohara; Shigeyuki Esumi; Yukio Fujiwara; Takahiro Yamamoto; Ken Uekawa; Kazutaka Ohta; Tatsuya Takezaki; Junichiro Kuroda; Naoki Shinojima; Tadashi Hamasaki; Akitake Mukasa (Human Cell, 2022)

Addition of ROCK inhibitors to prostaglandin derivative (PG) synergistically affects adipogenesis of the 3D spheroids of human orbital fibroblasts (HOFs) - Fumihito Hikage; Hanae Ichioka; Megumi Watanabe; Araya Umetsu; Hiroshi Ohguro; Yosuke Ida (Human Cell, 2022)

Mesenchymal stromal cells coated with anti-ACE2 antibodies might improve efficacy against COVID-19 - Nourhan Saied Bakry; Mai Abdelgawad; Ahmed Abdel-Latif; Ahmed Lotfy (Human Cell, 2022)

Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT - Takeyoshi Asakawa; Atsushi Yamada; Masumi Kugino; Tomokazu Hasegawa; Kentaro Yoshimura; Kiyohito Sasa; Mitsuhiro Kinoshita; Masakazu Nitta; Karin Nagata; Tomomi Sugiyama; Ryutaro Kamijo; Takahiro Funatsu (Human Cell, 2022)
Down’s syndrome is one of the most common human congenital genetic diseases and affected patients have increased risk of periodontal disease. To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down’s syndrome patient by use of SV40T-Ag and hTERT gene transfection. Expressions of SV40T-Ag and hTERT were observed in periodontal ligament cell-derived immortalized cells established from healthy (STPDL) and Down’s syndrome patient (STPDLDS) samples. Primary cultured periodontal ligament cells obtained from a healthy subject (pPDL) had a limited number of population doublings (< 40), while STPDL and STPDLDS cells continued to grow with more than 80 population doublings. Primary cultured periodontal ligament cells obtained from the patient showed a chromosome pattern characteristic of Down’s syndrome with trisomy 21, whereas STPDLDS samples showed a large number of abnormal chromosomes in those results. Gene expression analysis revealed that expression of DSCR-1 in STPDLDS is greater than that in STPDL. These results suggest that the newly established STPDLDS cell line may be a useful tool for study of periodontal disease in Down’s syndrome patients.

Exosomes derived from mesenchymal stem cells ameliorate renal fibrosis via delivery of miR-186-5p - Yiqiong Yang; Jing Wang; Yu Zhang; Xiuxiu Hu; Li Li; Pingsheng Chen (Human Cell, 2022)

Alpha-mangostin dephosphorylates ERM to induce adhesion and decrease surface stiffness in KG-1 cells - Thi Kieu Trang Phan; Thi Ly Do; Kouichi Tachibana; Takanori Kihara (Human Cell, 2021)

Therapeutics effect of mesenchymal stromal cells in reactive oxygen species-induced damages - Subodh Kumar; Ranjan Verma; Nishant Tyagi; Gurudutta Gangenahalli; Yogesh Kumar Verma (Human Cell, 2021)

Identification of diagnostic signatures in ulcerative colitis patients via bioinformatic analysis integrated with machine learning - Jiajie Lu; Zhiyuan Wang; Munila Maimaiti; Wenjia Hui; Adilai Abudourexiti; Feng Gao (Human Cell, 2021)

Risk factors for recurrent positive results of the nucleic acid amplification test for COVID-19 patients: a retrospective study - Wanwan Yi; Xuan Long; Jin Liu; LiShuai Shi; Zichen Chen; Jing Yang; Ziyu Yang; Zhongwei Lv; Hengwei Fan (Human Cell, 2021)
Positive retests of COVID-19 represent a public health concern because of the increased risk of transmission. This study explored whether factors other than the nucleic acid amplification test (NAAT) contribute to positive retest results. Patients with COVID-19 admitted to the Guanggu district of the Hubei Maternal and Child Health Hospital between February 17 and March 28, 2020, were retrospectively included. The patients were grouped into the negative ( n = 133) and positive ( n = 51) retest groups. The results showed that the proportion of patients presenting with cough was higher ( P < 0.001) and the proportion of patients with dyspnea was lower ( P = 0.018) in the positive than in the negative retest group. The positive retest group showed shorter durations between symptom onset and hospitalization ( P < 0.001) and symptom onset and the first positive NAAT ( P = 0.033). The positive retest group had higher basophil counts ( P = 0.023) and direct bilirubin ( P = 0.032) and chlorine concentrations ( P = 0.023) but lower potassium concentrations ( P = 0.001) than the negative retest group. Multivariable regression analysis showed that coughing (OR = 7.59, 95% CI 2.28–25.32, P = 0.001) and serum chloride concentrations (OR = 1.38, 95% CI 1.08–1.77, P = 0.010) were independently associated with a positive retest result. Coughing and serum chloride concentrations were independent risk factors for positive NAAT retest results. Patients with a hospital stay of < 2 weeks or a short incubation period should stay in isolation and be monitored to reduce transmission. These results could help identify patients who require closer surveillance.

Establishment and characterization of NCC-LGFMS1-C1: a novel patient-derived cell line of low-grade fibromyxoid sarcoma - Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Akane Sei; Jun Sugaya; Shintaro Iwata; Akihiko Yoshida; Akira Kawai; Tadashi Kondo (Human Cell, 2021)

The protective effects of Agomelatine against Aβ1-42 oligomers-induced cellular senescence mediated by SIRT6 and Agomelatine’s potential in AD treatment - Jian Wang; Bo Zheng; Shu Yang; Fang Wang; Zhonglin Wang; Jianhong Wang (Human Cell, 2021)

Correction to: HOXA11‑AS promotes the migration and invasion of hepatocellular carcinoma cells by inhibiting miR‑124 expression by binding to EZH2 - Wen‑long Zhang; Ya‑nan Zhao; Zhang‑zhen Shi; Gui‑ying Gu; Dan Cong; Chen Wei; Yuan‑song Bai (Human Cell, 2021)

Increase of serum uric acid levels associated with APOE ε2 haplotype: a clinico-genetic investigation and in vivo approach - Masatsune Ogura; Yu Toyoda; Masayuki Sakiyama; Yusuke Kawamura; Akiyoshi Nakayama; Yoshihide Yamanashi; Tappei Takada; Seiko Shimizu; Toshihide Higashino; Mayuko Nakajima; Mariko Naito; Asahi Hishida; et al. (Human Cell, 2021)
Elevated serum uric acid (SUA)—hyperuricemia—is caused by overproduction of urate or by its decreased renal and/or intestinal excretion. This disease, which is increasing in prevalence worldwide, is associated with both gout and metabolic diseases. Several studies have reported relationships between apolipoprotein E ( APOE ) haplotypes and SUA levels in humans; however, their results remain inconsistent. This prompted us to investigate the relationship between APOE polymorphisms and SUA levels. Our subjects were 5,272 Japanese men, premenopausal women, and postmenopausal women. Multiple linear regression analyses revealed the ε2 haplotype of APOE to be independently associated with higher SUA in men ( N = 1,726) and postmenopausal women ( N = 1,753), but not in premenopausal women ( N = 1,793). In contrast, the ε4 haplotype was little related to SUA levels in each group. Moreover, to examine the effect of Apoe deficiency on SUA levels, we conducted animal experiments using Apoe knockout mice, which mimics ε2/ε2 carriers. We found that SUA levels in Apoe knockout mice were significantly higher than those in wild-type mice, which is consistent with the SUA-raising effect of the ε2 haplotype observed in our clinico-genetic analyses. Further analyses suggested that renal rather than intestinal underexcretion of urate could be involved in Apoe deficiency-related SUA increase. In conclusion, we successfully demonstrated that the ε2 haplotype, but not the ε4 haplotype, increases SUA levels. These findings will improve our understanding of genetic factors affecting SUA levels.

Efficacy of IL-6 antagonists in the treatment of COVID-19 patients: further randomized controlled trials are warranted - Aditya K. Panda; Sunali Padhi; Abhijit Pati; Tapan Kumar Singh Nayak (Human Cell, 2021)

Long non-coding RNA CCAT2 drives the growth of laryngeal squamous cell carcinoma via regulating YAP activity - Yanzi Zang; Jing Li; Baoluo Wan; Yong Tai; Hongjian Liu; Qian Li; Yuzi Ji (Human Cell, 2021)

Role of long non-coding RNAs in Down syndrome patients: a transcriptome analysis study - Michele Salemi; Rossella Cannarella; Giovanna Marchese; Maria Grazia Salluzzo; Maria Ravo; Concetta Barone; Mariangela Lo Giudice; Aldo E. Calogero; Corrado Romano (Human Cell, 2021)

Long non-coding RNA ILF3-AS1 facilitates hepatocellular carcinoma progression by stabilizing ILF3 mRNA in an m6A-dependent manner - Changwen Bo; Na Li; Li He; Sujing Zhang; Yonghui An (Human Cell, 2021)

Construction of a ceRNA network and a genomic-clinicopathologic nomogram to predict survival for HBV-related HCC - Kang Huang; Zhongshan Lu; Ling Li; Guizhu Peng; Wei Zhou; Qifa Ye (Human Cell, 2021)

Overexpression of SMC4 predicts a poor prognosis in cervical cancer and facilitates cancer cell malignancy phenotype by activating NF-κB pathway - Hui He; Cui Zheng; Yunxian Tang (Human Cell, 2021)

Correction to: Mesenchymal stem cells‑derived exosomes modulate vascular endothelial injury via miR‑144‑5p/PTEN in intracranial aneurysm - Guojun Yang; Hao Qin; Bing Liu; Xinhong Zhao; Hang Yin (Human Cell, 2021)

Neurological effects of elevated levels of angiotensin II in COVID-19 patients - Mohammad Rafi Khezri; Morteza Ghasemnejad-Berenji (Human Cell, 2021)

Trelagliptin ameliorates oxygen–glucose deprivation/reperfusion (OGD/R)-induced mitochondrial dysfunction and metabolic disturbance of endothelial cells - Yatong Zhang; Chao Li; Yifang Pei; Li Zheng; Xuelin Sun; Zinan Zhao; Shuanghu Wang (Human Cell, 2021)

GATA3 as a regulator for naughty cancer-associated fibroblasts in the microenvironment of high-grade serous ovarian cancer - Amr Ahmed El-Arabey; Mohnad Abdalla (Human Cell, 2021)

Mesenchymal stem cell-derived exosomes block malignant behaviors of hepatocellular carcinoma stem cells through a lncRNA C5orf66-AS1/microRNA-127-3p/DUSP1/ERK axis - Hao Gu; Chao Yan; Haijun Wan; Lin Wu; Junjie Liu; Zhiqiang Zhu; Dazhi Gao (Human Cell, 2021)

Association of rs11549465 (C1772T) variant of hypoxia-inducible factor-1α with Covid-19 susceptibility. A population-based epidemiological study - Anamika Das; Maheswari Patra; Gunanidhi Dhangadamajhi (Human Cell, 2021)

One-carbon metabolism and global DNA methylation in mothers of individuals with Down syndrome - Cristiani Cortez Mendes; Bruna Lancia Zampieri; Lidia Maria Rebolho Batista Arantes; Matias Eliseo Melendez; Joice Matos Biselli; André Lopes Carvalho; Marcos Nogueira Eberlin; Maria Francesca Riccio; Hélio Vannucchi; Valdemir Melechco Carvalho; Eny Maria Goloni-Bertollo; Érika Cristina Pavarino (Human Cell, 2021)

Human umbilical cord-derived mesenchymal stem cell-exosomal miR-627-5p ameliorates non-alcoholic fatty liver disease by repressing FTO expression - Lidan Cheng; Peng Yu; Fangfang Li; Xueling Jiang; Xiaojuan Jiao; Yunfeng Shen; Xiaoyang Lai (Human Cell, 2021)

Association of ApoE isoforms with COVID-19 outcomes: a world-wide epidemiological study - Gunanidhi Dhangadamajhi; Swayamparna Mishra; Payal Mukherjee (Human Cell, 2021)

Highly expressed TLX1NB and NPSR1-AS1 lncRNAs could serve as diagnostic tools in colorectal cancer - Shaghayegh Dastjerdi; Mehdi Valizadeh; Rasoul Nemati; Mohammad Amin Honardoost; Nasrin Fattahi Dolatabadi; Atefeh Zamani; Hossein Tabatabaeian (Human Cell, 2021)

Establishment and characterization of NCC-MFS4-C1: a novel patient-derived cell line of myxofibrosarcoma - Yuki Yoshimatsu; Rei Noguchi; Ryuto Tsuchiya; Yooksil Sin; Takuya Ono; Jun Sugaya; Shintaro Iwata; Akihiko Yoshida; Akira Kawai; Tadashi Kondo (Human Cell, 2021)

Current status and future prospects of patient-derived induced pluripotent stem cells - Zhiqiang Wang; Jing Zheng; Ruolang Pan; Ye Chen (Human Cell, 2021)

Upregulation of lncRNA NIFK-AS1 in hepatocellular carcinoma by m6A methylation promotes disease progression and sorafenib resistance - Yi-tian Chen; Dan Xiang; Xiao-yue Zhao; Xiao-yuan Chu (Human Cell, 2021)

Effects of platinum-coexisting dopamine with X-ray irradiation upon human glioblastoma cell proliferation - Shinya Kato (Human Cell, 2021)

STAT3 inhibitor BBI608 enhances the antitumor effect of gefitinib on EGFR-mutated non-small cell lung cancer cells - Qian Wang; Bing Lu; Yi Zhang; Jing Yu; Jie Guo; Qianchi Zhou; Hong Lv; Yifeng Sun (Human Cell, 2021)

Emerging roles of circUBAP2 targeting miR-370-3p in proliferation, apoptosis, and invasion of papillary thyroid cancer cells - Hui Xiong; Jinsong Yu; Guangwei Jia; Yang Su; Jianliang Zhang; Qiu Xu; Xiaoxiong Sun (Human Cell, 2021)

FOXC2-AS1 stabilizes FOXC2 mRNA via association with NSUN2 in gastric cancer cells - Jijun Yan; Juntao Liu; Zhengbin Huang; Wenwei Huang; Jianfa Lv (Human Cell, 2021)

The Metaflammatory and Immunometabolic Role of Macrophages and Microglia in Diabetic Retinopathy - Honglian Wu; Mengqi Wang; Xiaorong Li; Yan Shao (Human Cell, 2021)

Mechanical stiffness softening and cell adhesion are coordinately regulated by ERM dephosphorylation in KG-1 cells - Takanori Kihara; Teru Matsumoto; Yoshihito Nakahashi; Kouichi Tachibana (Human Cell, 2021)

Establishment and characterization of novel patient-derived cell lines from giant cell tumor of bone - Yuki Yoshimatsu; Rei Noguchi; Ryuto Tsuchiya; Takuya Ono; Yooksil Sin; Sei Akane; Jun Sugaya; Tomoaki Mori; Suguru Fukushima; Akihiko Yoshida; Akira Kawai; Tadashi Kondo (Human Cell, 2021)

Differential immunohistochemical expression of paraoxonase-2 in actinic keratosis and squamous cell carcinoma - Davide Sartini; Roberto Campagna; Guendalina Lucarini; Veronica Pompei; Eleonora Salvolini; Monica Mattioli-Belmonte; Elisa Molinelli; Valerio Brisigotti; Anna Campanati; Tiziana Bacchetti; Gianna Ferretti; Annamaria Offidani; et al. (Human Cell, 2021)

MiR-222-3p induced by hepatitis B virus promotes the proliferation and inhibits apoptosis in hepatocellular carcinoma by upregulating THBS1 - Yongjie Sun; Peng Shi; Qi Wu; Bingqiang Liu; Zetao Yu; Hongtao Jia; Hong Chang (Human Cell, 2021)

LncRNA LINC01094 contributes to glioma progression by modulating miR-224-5p/CHSY1 axis - Luotong Liu; Qian Xu; Yu Xiong; Huajiang Deng; Jie Zhou (Human Cell, 2021)

Preparation, characterization, and antibacterial properties of hybrid nanofibrous scaffolds for cutaneous tissue engineering - Leila Mohammadzadeh; Mehrdad Mahkam; Abolfazl Barzegari; Abbas Karimi; Hossein Samadi Kafil; Roya Salehi; Reza Rahbarghazi (Human Cell, 2021)

RETRACTED ARTICLE: Long non-coding RNA VPS9D1-AS1 promotes growth of colon adenocarcinoma by sponging miR-1301-3p and CLDN1 - Wei Liu (Human Cell, 2021)

Research progress of DLX6-AS1 in human cancers - Yalan Luo; Peng Ge; Mengfei Wang; Haiyang Chen; Jiayue Liu; Tianfu Wei; Yuankuan Jiang; Jialin Qu; Hailong Chen (Human Cell, 2021)

Decreased prostasin expression is associated with aggressiveness of oral squamous cell carcinoma - Koji Yamamoto; Fumiki Yamashita; Makiko Kawaguchi; Aya Izumi; Takumi Kiwaki; Hiroaki Kataoka; Takeshi Kaneuji; Yoshihiro Yamashita; Tsuyoshi Fukushima (Human Cell, 2021)

Exosomal long non-coding RNA SOX2 overlapping transcript enhances the resistance to EGFR-TKIs in non-small cell lung cancer cell line H1975 - Dongbo Zhou; Zhi Xia; Mingxuan Xie; Ying Gao; Qiao Yu; Baimei He (Human Cell, 2021)

Mesenchymal stem cells-derived exosomes modulate vascular endothelial injury via miR-144-5p/PTEN in intracranial aneurysm - Guojun Yang; Hao Qin; Bing Liu; Xinhong Zhao; Hang Yin (Human Cell, 2021)

Regulation of KRAS protein expression by miR-544a and KRAS-LCS6 polymorphism in wild-type KRAS sporadic colon adenocarcinoma - Sonja Marinović; Anita Škrtić; Tina Catela Ivković; Mirko Poljak; Sanja Kapitanović (Human Cell, 2021)

Bone marrow mesenchymal stem cell-derived exosomes induce the Th17/Treg imbalance in immune thrombocytopenia through miR-146a-5p/IRAK1 axis - Yue He; Dexiang Ji; Wei Lu; Fei Li; Xianbao Huang; Ruibin Huang; Guoan Chen (Human Cell, 2021)

Correction to: Circular RNA circRNA_0000094 sponges microRNA‑223‑3p and up‑regulate F‑box and WD repeat domain containing 7 to restrain T cell acute lymphoblastic leukemia progression - Yan Hou; Junjie Sun; Jie Huang; Fengzhi Yao; Xuelian Chen; Bin Zhu (Human Cell, 2021)

Hsa_circ_0069244 acts as the sponge of miR-346 to inhibit non-small cell lung cancer progression by regulating XPC expression - Jiang Shi; Huan Wang; Wanlu Feng; Siyuan Huang; Jinlu An; Lifang Wang; Junguang Jiang (Human Cell, 2021)

LncRNA ITGB2-AS1 promotes the progression of clear cell renal cell carcinoma by modulating miR-328-5p/HMGA1 axis - Wensheng Zhang; Yigang Lu; Huibo Shi; Xungang Li; Zhuo Zhang; Xinxi Deng; Yang Yang; Bin Wan (Human Cell, 2021)

Circular RNA circ-CHI3L1.2 modulates cisplatin resistance of osteosarcoma cells via the miR-340-5p/LPAATβ axis - Zehua Zhang; Qiang Zhou; Fei Luo; Rui Zhou; Jianzhong Xu; Jun Xiao; Fei Dai; Lei Song (Human Cell, 2021)

Establishment and characterization of NCC-ssRMS2-C1: a novel patient-derived cell line of spindle cell/sclerosing rhabdomyosarcoma - Ryuto Tsuchiya; Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Takuya Ono; Akane Sei; Fumitaka Takeshita; Jun Sugaya; Fumihiko Nakatani; Akihiko Yoshida; Seiji Ohtori; Akira Kawai; et al. (Human Cell, 2021)

MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis - Muliang Ding; Bo Liu; Xia Chen; Zhengxiao Ouyang; Dan Peng; Yu Zhou (Human Cell, 2021)

Profiling heterogenous sizes of circulating tumor microemboli to track therapeutic resistance and prognosis in advanced gastric cancer - Yang Chen; Jiajia Yuan; Yanyan Li; Xue Li; Ying Yang; Jian Li; Yilin Li; Lin Shen (Human Cell, 2021)
Circulating tumor microemboli (CTM) aggregated by ≥ 2 circulating tumor cells (CTCs) are more migratory than single CTCs. Aside from the plasticity in their molecular characteristics, which have been considered tumor migration, CTM also possesses high size heterogeneity. This study, therefore, systematically investigated the heterogeneous sizes of CTM and their involvement in therapeutic resistance in 114 patients with advanced gastric cancer (GC) using a pre-established surface molecule-independent subtraction enrichment (SE)-iFISH strategy. CTM, which was pre-therapeutically detected in 33.3% of GC patients, can further form in another 34.78% of patients following chemo-/targeted therapies. The presence of CTM is relevant to liver metastasis as well as higher CTC levels (≥ 5/6 mL). Further size-based profiling of GC-CTM revealed that CTM with 2 CTCs (CTM 2 ) was the dominant subtype, accounting for 50.0% of all detected GC-CTMs. However, CTM with 3–4 CTCs (CTM 3–4 ) specifically associates with chemo-/targeted therapeutic resistance and inferior prognosis. Patients with ≥ 1 CTM 3–4 /6 mL have shorter median progression-free survival and median overall survival. Unlike CTM 2 and CTM 3–4 , which are detectable in pre-therapy and post-therapy, larger aggregated CTM ≥5 (CTM with ≥ 5 CTCs) was only intra-therapeutically detected in four HER2 + GC patients, of which three experienced liver metastases. Obtained results suggested that the cluster size of GC-CTM should be dynamically profiled beyond pre-therapeutic whole CTM enumeration in terms of chemo-/targeted resistance or metastasis monitoring. GC-CTM 3–4 could be a potential indicator of therapeutic resistance, while the dynamic presence of GC-CTM ≥5 implies liver metastasis in HER2 + GC patients.

Plexin domain containing protein 2 is more expressed within the invasive area of human colorectal cancer tissues - Yasuhiko Hamada; Akiko Eguchi; Kyosuke Tanaka; Masaki Katsurahara; Noriyuki Horiki; Misaki Nakamura; Mina Tenpaku; Motoh Iwasa; Masako Ichishi; Masatoshi Watanabe; Yoshiyuki Takei (Human Cell, 2021)

MiR-100-5p regulates cardiac hypertrophy through activation of autophagy by targeting mTOR - Junyi Zeng; Liang Wang; Jianqing Zhao; Zeqi Zheng; Jingtian Peng; Wan Zhang; Tong Wen; Jungang Nie; Lu Ding; Dasong Yi (Human Cell, 2021)

Smoking habits and risk of COVID-19 - Marco Rossato (Human Cell, 2021)

MicroRNA-1178-3p suppresses the growth of hepatocellular carcinoma by regulating transducin (beta)-like 1 X-linked receptor 1 - Qi Pan; Zigong Shao; Yijie Zhang; Hao Liu (Human Cell, 2021)

Suppressive role of microRNA-130b-3p in ferroptosis in melanoma cells correlates with DKK1 inhibition and Nrf2-HO-1 pathway activation - Yangying Liao; Xiaomin Jia; Yi Ren; Zhuoga Deji; Yuzhen Gesang; Ning Ning; Hao Feng; Hong Yu; An Wei (Human Cell, 2021)

Long non-coding RNA A1BG-AS1 promotes tumorigenesis in breast cancer by sponging microRNA-485-5p and consequently increasing expression of FLOT1 expression - Shibin Cai; Yi Zhou; Ying Pan; Pengpeng Liu; Kaijie Yu; Shuzheng Chen (Human Cell, 2021)

MiR-141-3p and miR-200a-3p are involved in Th17 cell differentiation by negatively regulating RARB expression - Leila Bahmani; Masoud Baghi; Maryam Peymani; Arash Javeri; Kamran Ghaedi (Human Cell, 2021)

The role of long non-coding RNA BCAR4 in human cancers - Qiong Yi; Zhongcheng Liu; Kun Zhang; Xuening Liu; Lifu Wang; Bin Geng; Yayi Xia (Human Cell, 2021)

miR-720 is a key regulator of glioma migration and invasion by controlling TARSL2 expression - Yinlong Liu; Kuan Jiang; Tongle Zhi; Xiupeng Xu (Human Cell, 2021)

C-reactive protein promotes tongue squamous cell carcinoma chemoresistance by inhibiting the activation of caspase-3/9 via the CD64/AKT/mTOR pathway - Xiaodong Yan; Meng Cao; Zhigang Wang; Shenglin Wang; Qinchao Chen (Human Cell, 2021)

Exosomal miR-486-5p derived from human placental microvascular endothelial cells regulates proliferation and invasion of trophoblasts via targeting IGF1 - Ruixia Ma; Zhijiang Liang; Xiaomei Shi; Linli Xu; Xiaowei Li; Jinhua Wu; Lina Zhao; Guocheng Liu (Human Cell, 2021)
Preeclampsia (PE) is a serious complication of pregnancy. Exosomes are known to be upregulated in PE. In this study, we sought to investigate the effect of miR-486-5p from human placental microvascular endothelial cells, on the function of trophoblast cells. To investigate the function of human placental microvascular endothelial cell (HPVEC)-derived exosomes on trophoblast cells, HPVECs were treated with hypoxia/reoxygenation (H/R). The separation efficiency of exosomes was determined by transmission electron microscopy, nanosight and Western blot. Cell Counting Kit-8, EdU staining, wound-healing, and transwell assay were performed to detect the effect of exosomally transferred miR-486-5p inhibitor on proliferation, migration and invasion of trophoblast cells. MiRDB and dual-luciferase report assay were used to find the target of miR-486-5p. Our data revealed that miR-486-5p was significantly upregulated in H/R-treated HPVEC-Exo, and miR-486-5p was enriched in HPVEC-Exo. miR-486-5p inhibitor carried by HPVEC-Exo significantly inhibited the proliferation, migration and invasion of trophoblast cells. Insulin-like growth factor 1 (IGF1) was found to be the target of miR-486-5p, and IGF1 overexpression notably reversed the effect of miR-486-5p inhibitor from HPVEC-Exo on trophoblast cell function. In summary, H/R-treated HPVEC-derived exosomally expressing miR-486-5p inhibitor significantly inhibited the proliferation, migration and invasion of trophoblast cells via downregulation of IGF1. The findings from the present study may be useful in the development of treatments for PE.

Effects of multicycle gonadotropin-releasing hormone antagonist protocols on oxidative stress of follicular fluid and ovarian granulosa cells - Yucong Ma; Zhiming Zhao; Guimin Hao; Na Cui; Yanli Fan; Yucong Cao; Zhanwang Tan; Jingran Geng; Lijie Fan; Huilan Du; Bulang Gao (Human Cell, 2021)

CNTNAP2 gene polymorphisms in autism spectrum disorder and language impairment among Bangladeshi children: a case–control study combined with a meta-analysis - Mohammad Sarowar Uddin; Atkia Azima; Md. Abdul Aziz; Tutun Das Aka; Sarah Jafrin; Md. Shalahuddin Millat; Shafayet Ahmed Siddiqui; Md. Giash Uddin; Md. Saddam Hussain; Mohammad Safiqul Islam (Human Cell, 2021)

PIWI proteins and piRNAs in cervical cancer: a propitious dart in cancer stem cell-targeted therapy - Midhunaraj Kunnummal; Mary Angelin; Ani V. Das (Human Cell, 2021)

Mesenchymal stem cells: ideal seeds for treating diseases - Guanwen Gao; Chenyang Fan; Weiquan Li; Runzhang Liang; Chuzhong Wei; Xiaojie Chen; Yue Yang; Yueyuan Zhong; Yingqi Shao; Yi Kong; Zesong Li; Xiao Zhu (Human Cell, 2021)

Correction to: Circ0001320 inhibits lung cancer cell growth and invasion by regulating TNFAIP1 and TPM1 expression through sponging miR‑558 - Yong Mao; Jia‑xi He; Mei Zhu; Yong‑quan Dong; Jian‑xing He (Human Cell, 2021)

Correction to: Smoking habits correlate with the defense against SARS‑CoV‑2 infection in the Indian population - Bishwaranjan Purohit; Aditya K. Panda (Human Cell, 2021)

The potential roles of lncRNAs DUXAP8, LINC00963, and FOXD2-AS1 in luminal breast cancer based on expression analysis and bioinformatic approaches - Maedeh Arabpour; Sepideh Mehrpour Layeghi; Javad Tavakkoly Bazzaz; Mohammad Mehdi Naghizadeh; Keivan Majidzadeh-A; Abbas Shakoori (Human Cell, 2021)

Neural extracellular vesicle-derived miR-17 in blood as a potential biomarker of subthreshold depression - Yusuke Mizohata; Hiroyuki Toda; Minori Koga; Taku Saito; Masanori Fujita; Tetsuya Kobayashi; Shin Hatakeyama; Yuji Morimoto (Human Cell, 2021)

Porphyrin accumulation in humans with common dysfunctional variants of ABCG2, a porphyrin transporter: potential association with acquired photosensitivity - Masayuki Sakiyama; Hirotaka Matsuo; Yu Toyoda; Yuiko Yonekura; Takahiro Ishikawa; Akiyoshi Nakayama; Toshihide Higashino; Yusuke Kawamura; Norihiro Fujimoto; Nariyoshi Shinomiya; Takahiro Satoh (Human Cell, 2021)
Photosensitivity is a skin reaction disorder mediated by phototoxic and/or photoallergic mechanisms. The accumulation of porphyrins is generally considered to induce phototoxicity. ATP-binding cassette subfamily G member 2 (ABCG2) has been identified as a transporter of porphyrins and its common variants—p.Gln126Ter (rs72552713) and p.Gln141Lys (rs2231142)—reportedly decrease the function of porphyrin transport in vitro; however, the physiological importance of ABCG2 as a porphyrin transporter remains to be fully elucidated. We herein investigated whether ABCG2 dysfunction could lead to porphyrin accumulation and photosensitivity in Japanese subjects, and found it to be significantly correlated with erythrocyte protoporphyrin levels ( P = 0.012). This appears to be the first clinical finding of ABCG2 dysfunction-associated protoporphyrin accumulation in humans. We divided the patients into a chronic actinic dermatosis (CAD) group and a non-CAD group. CAD was diagnosed based on the criteria of reduced minimal erythema doses to ultraviolet B (UVB) and/or ultraviolet A (UVA). The non-CAD group was composed of patients who exhibited normal reactions to UVB and UVA on phototesting, but had histories of recurrent erythema/papules on sun-exposed areas. Estimated ABCG2 function according to ABCG2 genotypes in the non-CAD group was significantly lower than in the general Japanese population ( P = 0.045). In contrast, no difference was found in ABCG2 function between the CAD group and the general population, suggesting that ABCG2 dysfunction might be a genetic factor in non-CAD patients with clinical photosensitivity. In this context, genetic dysfunction of ABCG2 might be an overlooked pathological etiology of “photosensitivity of unknown cause.”

Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis - Yuan Huang; Yabing Zheng; Xiying Shao; Lei Shi; Guangliang Li; Ping Huang (Human Cell, 2021)

Smoking habits correlate with the defense against SARS-CoV-2 infection in the Indian population - Bishwaranjan Purohit; Aditya K. Panda (Human Cell, 2021)

Establishment and characterization of NCC-MFS3-C1: a novel patient-derived cell line of myxofibrosarcoma - Ryuto Tsuchiya; Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Takuya Ono; Akane Sei; Fumitaka Takeshita; Jun Sugaya; Shintaro Iwata; Akihiko Yoshida; Seiji Ohtori; Akira Kawai; et al. (Human Cell, 2021)

The role of TNF-α induced protein 1 in the activation of pro-apoptotic proteins - Xiaoren Tang; Thanarut Tangkham; Bushra Aljahdali; Sean Lee; Mingfang Su; Serge Dibart (Human Cell, 2021)

Circ_0067835 regulates allergic inflammatory response in type-2 innate lymphoid cells in allergic rhinitis (AR) via miR-155/GATA3 - Xunshuo Jiang; Taojian Huang; Hongbing Liu; Xubo Chen; Hao Zhang; Ke Liu; Jianjian Deng; Chunping Yang (Human Cell, 2021)

Current insight into the functions of microRNAs in common human hair loss disorders: a mini review - Sujay Paul; Iván Licona-Vázquez; Francisco I. Serrano-Cano; Natalia Frías-Reid; Carolina Pacheco-Dorantes; Surajit Pathak; Samik Chakraborty; Aashish Srivastava (Human Cell, 2021)

IGF-1R anti-idiotypic antibody antagonist exhibited anti-ovarian cancer bioactivity and reduced cisplatin resistance - Zhang Weiwei; Xie Ya; Wang Wenwen; Jia Jia; Bai Jing; Zhang Ruitao; Wang Chunfang; Guo Ruixia (Human Cell, 2021)

The role of macrophages in anti-tumor immune responses: pathological significance and potential as therapeutic targets - Hirotake Tsukamoto; Yoshihiro Komohara; Hiroyuki Oshiumi (Human Cell, 2021)

Nisin and non-essential amino acids: new perspective in differentiation of neural progenitors from human-induced pluripotent stem cells in vitro - Elahe Eftekhari; Marzieh Ghollasi; Raheleh Halabian; Maryam Soltanyzadeh; Seyed Ehsan Enderami (Human Cell, 2021)

Long non-coding RNA FGD5-AS1 enhances osteosarcoma cell proliferation and migration by targeting miR-506-3p/RAB3D axis - Congda Li; Xiangbo Lin; Caiyun Zhang; Lei Wan; Jijun Yin; Bin Wang (Human Cell, 2021)

S100A14 inhibits cell growth and epithelial–mesenchymal transition (EMT) in prostate cancer through FAT1-mediated Hippo signaling pathway - Shaoqin Jiang; Yaru Zhu; Zhenlin Chen; Zhangcheng Huang; Bingqiao Liu; Yue Xu; Zhihao Li; Zequn Lin; Mengqiang Li (Human Cell, 2021)

Microvesicles derived from human umbilical cord mesenchyme promote M2 macrophage polarization and ameliorate renal fibrosis following partial nephrectomy via hepatocyte growth factor - Tao Du; Guanqun Ju; Jun Zhou; Liang Zhong; Lu Rong; Wenxia Chen; Xiaoli Zhang; Ruijin Zhou; Degang Ding; Tongyu Ji (Human Cell, 2021)

Correction to: Diethylhexyl phthalate (DEHP) regulates the proliferation and chemosensitivity of esophageal squamous cell carcinoma cells via regulation of PTEN - Jian Zhu; Xuesong Zhang; Bin Wen; Jing Chen; Yuntian Lu; Weijun Xu (Human Cell, 2021)

Neuropilin-1 may be responsible for retinal findings in patients with COVID-19 - Amr Ahmed El‑Arabey; Mohnad Abdalla (Human Cell, 2021)

TLR3 (rs3775291) variant is not associated with SARS-CoV-2 infection and related mortality: a population-based correlation analysis - Abhijit Pati; Sunali Padhi; Sanskruti Chaudhury; Aditya K. Panda (Human Cell, 2021)

Proposal for the designation of the natural killer antigens-positive γδ T-cell subset as γδ NKT-cells: nomenclature based on immunoprofile - Yoshinobu Matsuo; Takahiro Tsujimura; Hans G. Drexler (Human Cell, 2021)

LINGO-1 shRNA protects the brain against ischemia/reperfusion injury by inhibiting the activation of NF-κB and JAK2/STAT3 - Jiaying Zhu; Zhu Zhu; Yipin Ren; Yukang Dong; Yaqi Li; Xiulin Yang (Human Cell, 2021)
LINGO-1 may be involved in the pathogenesis of cerebral ischemia. However, its biological function and underlying molecular mechanism in cerebral ischemia remain to be further defined. In our study, middle cerebral artery occlusion/reperfusion (MACO/R) mice model and HT22 cell oxygen–glucose deprivation/reperfusion (OGD/R) were established to simulate the pathological process of cerebral ischemia in vivo and in vitro and to detect the relevant mechanism. We found that LINGO-1 mRNA and protein were upregulated in mice and cell models. Down-regulation LINGO-1 improved the neurological symptoms and reduced pathological changes and the infarct size of the mice after MACO/R. In addition, LINGO-1 interference alleviated apoptosis and promoted cell proliferation in HT22 of OGD/R. Moreover, down-regulation of LINGO-1 proved to inhibit nuclear translocation of p-NF-κB and reduce the expression level of p-JAK2 and p-STAT3. In conclusion, our data suggest that shLINGO-1 attenuated ischemic injury by negatively regulating NF-KB and JAK2/STAT3 pathways, highlighting a novel therapeutic target for ischemic stroke.

LINC00200 contributes to the chemoresistance to oxaliplatin of gastric cancer cells via regulating E2F1/RAD51 axis - Mengxin Lin; Meifang Xu; Zongbin Xu; Zongqi Weng; Bingqiang Lin; Yanqin Lan; Qing Liu; Xiaoyan Lin; Jie Pan (Human Cell, 2021)

Circular RNA circ-PTEN elevates PTEN inhibiting the proliferation of non-small cell lung cancer cells - Yuan Wang; Zibo Wang; Jibin Lu; Hongyan Zhang (Human Cell, 2021)

Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes and PEDF secretion - Jinhui Zhang; Wenya Fan; Lingling Neng; Bei Chen; Bin Zuo; Wei Lu (Human Cell, 2021)

Human TERT promoter polymorphism rs2853669 is associated with cancers: an updated meta-analysis - Md. Abdul Aziz; Sarah Jafrin; Mohammad Safiqul Islam (Human Cell, 2021)

Diethylhexyl phthalate (DEHP) regulates the proliferation and chemosensitivity of esophageal squamous cell carcinoma cells via regulation of PTEN - Jian Zhu; Xuesong Zhang; Bin Wen; Jing Chen; Yuntian Lu; Weijun Xu (Human Cell, 2021)

Novel cell-based therapies in inflammatory bowel diseases: the established concept, promising results - Nikoo Hossein-khannazer; Shukoofeh Torabi; Ramin Hosseinzadeh; Shabnam Shahrokh; Hamid Asadzadeh Aghdaei; Arash Memarnejadian; Nadir Kadri; Massoud Vosough (Human Cell, 2021)

The outstanding role of miR-132-3p in carcinogenesis of solid tumors - Milad Rafat; Mahta Moraghebi; Masoumeh Afsa; Kianoosh Malekzadeh (Human Cell, 2021)

Down-regulation of long non-coding RNA XIST aggravates sepsis-induced lung injury by regulating miR-16-5p - Xiaofei Song; Linyu Li; Yaying Zhao; Yucheng Song (Human Cell, 2021)

Correction to: Heterogenous chemosensitivity of a panel of organoid lines derived from small cell neuroendocrine carcinoma of the uterine cervix - Mie Tanaka; Jumpei Kondo; Kensuke Kaneko; Hiroko Endo; Kunishige Onuma; Roberto Coppo; Masamune Masuda; Shoji Kamiura; Kiyoshi Yoshino; Yutaka Ueda; Hideaki Kakeya; Tadashi Kimura; et al. (Human Cell, 2021)

Establishment and characterization of 38 novel patient-derived primary cancer cell lines using multi-region sampling revealing intra-tumor heterogeneity of gallbladder carcinoma - Feiling Feng; Qingbao Cheng; Bin Li; Chen Liu; Huizhen Wang; Bin Li; Xiaoya Xu; Yong Yu; Zishuo Chen; Xiaobing Wu; Hua Dong; Kaijian Chu; et al. (Human Cell, 2021)
Gallbladder carcinoma (GBC) is a lethal biliary tract malignant neoplasm. Patient-derived primary cancer cell lines (PDPCs) are appropriate models to explore biological characteristics and potential therapeutics; however, there is a lack of PDPCs in GBC. In this study, we aimed to establish and characterize the GBC PDPCs, and further investigated the intra-tumor heterogeneity (ITH). Multi-region sampling (3–9 regions) of the operable tumor tissue samples was used to establish PDPCs. Short tandem repeat genotyping for cell authentication and karyotyping was performed, followed by whole-exome sequencing and RNA sequencing to assess the ITH at the genetic and transcriptional levels, respectively. Thirty-eight PDPCs were successfully established from seven GBC patients and characterized. ITH was observed with a median of 38.3% mutations being heterogeneous (range, 26.6–59.4%) across all patients. Similar with other tumor types, TP53 mutations were always truncal. In addition, there were three genes, KMT2C , CDKN2A , and ARID1A , with truncal mutations in at least two patients. A median of 370 differentially expressed genes (DEGs) was identified per patient. Distinct expression patterns were observed between major histocompatibility complex (MHC) class I and II genes. We found the expression of MHC class II genes in the PDPC samples was closely regulated by CIITA , while that of MHC class I genes were not correlated with CIITA expression. The PDPCs established from GBC patients can serve as novel in vitro models to identify the ITH, which may pave a crucial molecular foundation for enhanced understanding of tumorigenesis and progression.

SP1-induced overexpression of LINC00520 facilitates non-small cell lung cancer progression through miR-577/CCNE2 pathway and predicts poor prognosis - Ji-fang Wang; Zhuo-na Xi; Hong-jian Su; Zhen Bao; Ya-hong Qiao (Human Cell, 2021)

The optimal number of embryo cells for effective pregnancy and decrease of multiple pregnancy rate in frozen-thawed embryo transfer - Xu-Li Zhu; Zhi-Ming Zhao; Yuan-Jie Du; Liang Zhou; Yue Wang; Qing-Yun Sun; Gui-Min Hao; Bu-Lang Gao (Human Cell, 2021)

Isolation of adipose tissue-derived stem cells by direct membrane migration and expansion for clinical application - Yoshinobu Matsuo; Hiromichi Morita; Hisakazu Yamagishi; Mitsutoshi Nakamura; Yasuhiro Takeshima; Ichiro Nakagawa; Jiro Imanishi; Takahiro Tsujimura (Human Cell, 2021)

COVID-19 vaccines: rapid development, implications, challenges and future prospects - Shivaji Kashte; Arvind Gulbake; Saadiq F. El-Amin III; Ashim Gupta (Human Cell, 2021)
COVID-19 has affected millions of people and put an unparalleled burden on healthcare systems as well as economies throughout the world. Currently, there is no decisive therapy for COVID-19 or related complications. The only hope to mitigate this pandemic is through vaccines. The COVID-19 vaccines are being developed rapidly, compared to traditional vaccines, and are being approved via Emergency Use Authorization (EUA) worldwide. So far, there are 232 vaccine candidates. One hundred and seventy-two are in preclinical development and 60 in clinical development, of which 9 are approved under EUA by different countries. This includes the United Kingdom (UK), United States of America (USA), Canada, Russia, China, and India. Distributing vaccination to all, with a safe and efficacious vaccine is the leading priority for all nations to combat this COVID-19 pandemic. However, the current accelerated process of COVID-19 vaccine development and EUA has many unanswered questions. In addition, the change in strain of SARS-CoV-2 in UK and South Africa, and its increasing spread across the world have raised more challenges, both for the vaccine developers as well as the governments across the world. In this review, we have discussed the different type of vaccines with examples of COVID-19 vaccines, their rapid development compared to the traditional vaccine, associated challenges, and future prospects.

Heterogenous chemosensitivity of a panel of organoid lines derived from small cell neuroendocrine carcinoma of the uterine cervix - Mie Tanaka; Jumpei Kondo; Kensuke Kaneko; Hiroko Endo; Kunishige Onuma; Roberto Coppo; Masamune Masuda; Shoji Kamiura; Kiyoshi Yoshino; Yutaka Ueda; Hideaki Kakeya; Tadashi Kimura; et al. (Human Cell, 2021)

Establishment and characterization of NCC-DDLPS3-C1: a novel patient-derived cell line of dedifferentiated liposarcoma - Ryuto Tsuchiya; Yuki Yoshimatsu; Rei Noguchi; Takuya Ono; Akane Sei; Fumitaka Takeshita; Jun Sugaya; Suguru Fukushima; Akihiko Yoshida; Seiji Ohtori; Akira Kawai; Tadashi Kondo (Human Cell, 2021)

Circular RNA circRNA_0000094 sponges microRNA-223-3p and up-regulate F-box and WD repeat domain containing 7 to restrain T cell acute lymphoblastic leukemia progression - Yan Hou; Junjie Sun; Jie Huang; Fengzhi Yao; Xuelian Chen; Bin Zhu; Dongchi Zhao (Human Cell, 2021)

Stress-induced phosphoprotein 1 facilitates breast cancer cell progression and indicates poor prognosis for breast cancer patients - Lizhi Lin; Jialiang Wen; Bangyi Lin; Erjie Xia; Chen Zheng; Lechi Ye; Yinghao Wang; Ouchen Wang; Yizuo Chen (Human Cell, 2021)

Small-sized extracellular vesicles (EVs) derived from acute myeloid leukemia bone marrow mesenchymal stem cells transfer miR-26a-5p to promote acute myeloid leukemia cell proliferation, migration, and invasion - Dexiang Ji; Yue He; Wei Lu; Yanyan Rong; Fei Li; Xianbao Huang; Ruibin Huang; Yanxia Jiang; Guoan Chen (Human Cell, 2021)

Identification of characteristic proteins at late-stage erythroid differentiation in vitro - Koji Funato; Takaaki Abe; Ryo Kurita; Yoshihisa Watanabe; Yukio Nakamura; Shigeki Miyata; Yusuke Furukawa; Masahiro Satake (Human Cell, 2021)

Association of TLR3 functional variant (rs3775291) with COVID-19 susceptibility and death: a population-scale study - Gunanidhi Dhangadamajhi; Ronnaly Rout (Human Cell, 2021)

LncRNA Rian ameliorates sevoflurane anesthesia-induced cognitive dysfunction through regulation of miR-143-3p/LIMK1 axis - Yang Yu; Wei Zhang; Dengyan Zhu; Haitao Wang; Hua Shao; Yue Zhang (Human Cell, 2021)

Establishment and characterization of NCC-SS4-C1: a novel patient-derived cell line of synovial sarcoma - Ryuto Tsuchiya; Yuki Yoshimatsu; Rei Noguchi; Takuya Ono; Akane Sei; Fumitaka Takeshita; Jun Sugaya; Shintaro Iwata; Akihiko Yoshida; Seiji Ohtori; Akira Kawai; Tadashi Kondo (Human Cell, 2021)

Automatic discrimination of human hematopoietic tumor cell lines using a combination of imaging flow cytometry and convolutional neural network - Yoshikazu Matsuoka; Ryusuke Nakatsuka; Tatsuya Fujioka (Human Cell, 2021)

The novel circular RNA circ-PGAP3 retards cervical cancer growth by regulating the miR-769-5p/p53 axis - Tian Jun; Wang Chen; Cheng Hailing; Wang Ning; Cao Qinxue (Human Cell, 2021)

FlowCell-enriched circulating tumor cells as a predictor of lung cancer metastasis - Yan Lu; Yushuang Zheng; Yuhong Wang; Dongmei Gu; Jun Zhang; Fang Liu; Kai Chen; Lingchuan Guo (Human Cell, 2021)
Lung cancer is the most fetal malignancy due to the high rate of metastasis and recurrence after treatment. A considerable number of patients with early-stage lung cancer relapse due to overlooked distant metastasis. Circulating tumor cells (CTCs) are tumor cells in blood circulation that originated from primary or metastatic sites, and it has been shown that CTCs are critical for metastasis and prognosis in various type of cancers. Here, we employed novel method to capture, isolate and classify CTC with FlowCell system and analyzed the CTCs from a cohort of 302 individuals. Our results illustrated that FlowCell-enriched CTCs effectively differentiated benign and malignant lung tumor and the total CTC counts increased as the tumor developed. More importantly, we showed that CTCs displayed superior sensitivity and specificity to predict lung cancer metastasis in comparison to conventional circulating biomarkers. Taken together, our data suggested CTCs can be used to assist the diagnosis of lung cancer as well as predict lung cancer metastasis. These findings provide an alternative means to screen early-stage metastasis.

Transcriptomic analysis reveals tumor stage- or grade-dependent expression of miRNAs in serous ovarian cancer - Caglar Berkel; Ercan Cacan (Human Cell, 2021)

Overexpression of ring finger protein 20 inhibits the progression of liver fibrosis via mediation of histone H2B lysine 120 ubiquitination - Shanshan Chen; Xuan Dai; Hong Li; Yuhan Gong; Yueyue Zhao; Haijun Huang (Human Cell, 2021)

Establishment and characterization of a novel cell line, NCC-DDLPS2-C1, derived from a patient with dedifferentiated liposarcoma - Rei Noguchi; Yuki Yoshimatsu; Takuya Ono; Akane Sei; Kaoru Hirabayashi; Iwao Ozawa; Kazutaka Kikuta; Tadashi Kondo (Human Cell, 2021)

Long non-coding RNA nuclear paraspeckle assembly transcript 1 promotes activation of T helper 2 cells via inhibiting STAT6 ubiquitination - Shuman Huang; Dong Dong; Yaqian Zhang; Zhuo Chen; Jing Geng; Yulin Zhao (Human Cell, 2021)

Ovarian cancer immunotherapy of NF-κB may have a dark side - Amr Ahmed El-Arabey; Mohnad Abdalla; Adel Rashad Abd-Allah (Human Cell, 2021)

Elevated levels of expression of cytochrome P450 3A4 in a human liver epithelial cell line in differentiation-inducing conditions - Taisuke Yamazaki; Takayoshi Tokiwa (Human Cell, 2021)

Targeted deletion of HAI-1 increases prostasin proteolysis but decreases matriptase proteolysis in human keratinocytes - Dajun D. Lu; Yayun Gu; Sheng-Wen A. Li; Robert J. Barndt; Shih-Ming Huang; Jehng-Kang Wang; Hui Chen Su; Michael D. Johnson; Chen-Yong Lin (Human Cell, 2021)

Exosomal miR-21-5p contributes to ovarian cancer progression by regulating CDK6 - Jian Cao; Yuan Zhang; Juan Mu; Dazhen Yang; Xiaoyan Gu; Jing Zhang (Human Cell, 2021)

Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells - Hsueh-Chun Wang; Kwei-Yan Liu; Li-Ting Wang; Shih-Hsien Hsu; Shao-Chun Wang; Shau-Ku Huang (Human Cell, 2021)

Fndc5 knockdown significantly decreased the expression of neurotrophins and their respective receptors during neural differentiation of mouse embryonic stem cells - Reihane Ebadi; Farzaneh Rabiee; DorMohammad Kordi-Tamandani; Mohammad Hossein Nasr-Esfahani; Kamran Ghaedi (Human Cell, 2021)

Malaria link of hypertension: a hidden syndicate of angiotensin II, bradykinin and sphingosine 1-phosphate - Gunanidhi Dhangadamajhi; Shailja Singh (Human Cell, 2021)

Long non-coding RNA NEAT1 regulates endothelial functions in subclinical hypothyroidism through miR-126/TRAF7 pathway - Li Wang; Jingzhi Liu; Kunna Lu; Yuyu Qiu; Xiaoxia Li; Feng Yue; Xinhuan Zhang (Human Cell, 2021)

Clinical features and prognostic factors of patients with COVID-19 in Henan Province, China - Qianqian Zhang; Zheng Wang; Yanping Lv; Jiang Zhao; Qiang Dang; Dongfeng Xu; Dongxiang Zhao; Haiyang Liu; Ziqi Wang; Xingru Zhao; Zhiwei Xu; Xiaoju Zhang (Human Cell, 2021)

Characterization of stem cells from human ovarian follicular fluid; a potential source of autologous stem cell for cell-based therapy - Ruttachuk Rungsiwiwut; Pranee Numchaisrika; Paweena Thuwanut; Kamthorn Pruksananonda (Human Cell, 2021)

mTOR inhibition: a double-edged sword in patients with COVID-19? - Morteza Ghasemnejad-Berenji (Human Cell, 2021)

ACE2 mutation might explain lower COVID-19 burden in malaria endemic areas - Auley De; Aparna Tiwari; Manoswini Dash; Abhinav Sinha (Human Cell, 2021)

RING-finger protein 5 promotes hepatocellular carcinoma progression and predicts poor prognosis - Yi Zhang; Jie Li; Huayang Chen; Changmao Zhang; Song You; Yue Zhao; Xiaoning Lin; Yaqi Yu; Fei Fang; Ting Fang; Xiaomin Wang (Human Cell, 2021)

Long noncoding RNA LINC00520 accelerates lung adenocarcinoma progression via miR-1252-5p/FOXR2 pathway - Xinyue Chen; Huimin Chen; Miaowen Liu; Jianping Xiong; Zhiwang Song (Human Cell, 2021)

MiR-27b-3p inhibits the progression of renal fibrosis via suppressing STAT1 - Lin Bai; Yongtao Lin; Juan Xie; Yiyuan Zhang; Hongwu Wang; Donghui Zheng (Human Cell, 2021)
Renal fibrosis is a pathologic change in chronic kidney disease (CKD). MicroRNAs (miRNAs) have been shown to play an important role in the development of renal fibrosis. However, the biological role of miR-27b-3p in renal fibrosis remains unclear. Thus, this study aimed to investigate the role of miR-27b-3p in the progression of renal fibrosis. In this study, HK-2 cells were stimulated with transforming growth factor (TGF)-β1 for mimicking fibrosis progression in vitro. The unilateral ureteric obstruction (UUO)-induced mice renal fibrosis in vivo was established as well. The results indicated that the overexpression of miR-27b-3p significantly inhibited epithelial-to-mesenchymal transition (EMT) in TGF-β1-stimulated HK-2 cells, as shown by the decreased expressions of α-SMA, collagen III, Fibronectin and Vimentin. In addition, overexpression of miR-27b-3p markedly decreased TGF-β1-induced apoptosis in HK-2 cells, as evidenced by the decreased levels of Fas, active caspase 8 and active caspase 3. Meanwhile, dual-luciferase assay showed that miR-27b-3p downregulated signal transducers and activators of transcription 1 (STAT1) expression through direct binding with the 3′-UTR of STAT1. Furthermore, overexpression of miR-27b-3p attenuated UUO-induced renal fibrosis via downregulation of STAT1, α-SMA and collagen III. In conclusion, miR-27b-3p overexpression could alleviate renal fibrosis via suppressing STAT1 in vivo and in vitro. Therefore, miR-27b-3p might be a promising therapeutic target for the treatment of renal fibrosis.

High-resolution melting effectively pre-screens for TP53 mutations before direct sequencing in patients with diffuse glioma - Kiyotaka Saito; Kiyotaka Yokogami; Kazunari Maekawa; Yuichiro Sato; Shinji Yamashita; Fumitaka Matsumoto; Asako Mizuguchi; Hideo Takeshima (Human Cell, 2021)

Uptake of fluorescent d- and l-glucose analogues, 2-NBDG and 2-NBDLG, into human osteosarcoma U2OS cells in a phloretin-inhibitable manner - Tetsuya Ogawa; Ayako Sasaki; Koki Ono; Shusa Ohshika; Yasuyuki Ishibashi; Katsuya Yamada (Human Cell, 2021)
Mammalian cells take in d -glucose as an essential fuel as well as a carbon source. In contrast, l -glucose, the mirror image isomer of d -glucose, has been considered merely as a non-transportable/non-metabolizable control for d -glucose. We have shown that 2-[ N -(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG), a d -glucose analogue combining a fluorophore NBD at the C-2 position, is useful as a tracer for monitoring d -glucose uptake through glucose transporters (GLUTs) into mammalian cells. To more precisely evaluate the stereoselectivity of 2-NBDG uptake, we developed an l -glucose analogue 2-NBDLG, the mirror-image isomer of 2-NBDG. Interestingly, 2-NBDLG was taken up into mouse insulinoma MIN6 cells showing nuclear heterogeneity, a cytological feature of malignancy, while remaining MIN6 cells only exhibited a trace amount of 2-NBDLG uptake. The 2-NBDLG uptake into MIN6 cells was abolished by phloretin, but persisted under blockade of major mammalian glucose transporters. Unfortunately, however, no such uptake could be detected in other tumor cell lines. Here we demonstrate that human osteosarcoma U2OS cells take in 2-NBDLG in a phloretin-inhibitable manner. The uptake of 2-NBDG, and not that of 2-NBDLG, into U2OS cells was significantly inhibited by cytochalasin B, a potent GLUT inhibitor. Phloretin, but neither phlorizin, an inhibitor of sodium-glucose cotransporter (SGLT), nor a large amount of d / l -glucose, blocked the 2-NBDLG uptake. These results suggest that a phloretin-inhibitable, non-GLUT/non-SGLT, possibly non-transporter-mediated yet unidentified mechanism participates in the uptake of the fluorescent l -glucose analogue in two very different tumor cells, the mouse insulinoma and the human osteosarcoma cells.

Upregulation of miR-144-3p protects myocardial function from ischemia–reperfusion injury through inhibition of TMEM16A Ca2+-activated chloride channel - Guoxun Yang; Xiao Tang; Ling Tan; Danpeng Nong; Peng Yang; Haien Ning (Human Cell, 2021)

PI3K/AKT signaling pathway: a possible target for adjuvant therapy in COVID-19 - Mohammad Rafi Khezri (Human Cell, 2021)

CBX8 acts as an independent RNA-binding protein to regulate the maturation of miR-378a-3p in colon cancer cells - Xin Song; Wu Ning; Jinwei Niu; Guochao Zhang; Haibin Liu; Lei Zhou (Human Cell, 2021)

SH2 domain-containing protein tyrosine phosphatase-2 (SHP-2) prevents cardiac remodeling after myocardial infarction through ERK/SMAD signaling pathway - Yong-Gang Lu; He Tan; Qian Ma; Xin-Xin Li; Jia Cui; Xue Zhang; Xue-Lei Liang; Yan-Qing Tie (Human Cell, 2021)

MiR-501-3p promotes osteosarcoma cell proliferation, migration and invasion by targeting BCL7A - Jinliang Dai; Lin Lu; Lixin Kang; Jian Zhang (Human Cell, 2021)

Development of a cell-based pathway modulator screening system to screen the targeted cancer therapeutic candidates - Ponmathi Panneerpandian; Kumaresan Ganesan (Human Cell, 2021)

SP1-induced AFAP1-AS1 contributes to proliferation and invasion by regulating miR-497-5p/CELF1 pathway in nasopharyngeal carcinoma - Hui Jin; Gengtian Liang; Liping Yang; Li Liu; Binru Wang; Fengqin Yan (Human Cell, 2021)

MiR-30a suppresses clear cell renal cell carcinoma proliferation and metastasis by targeting LRP6 - Yanjun Ren; Li Zhang; Wei Zhang; Yikai Gao (Human Cell, 2021)

LncRNA MAFG-AS1 regulates miR-125b-5p/SphK1 axis to promote the proliferation, migration, and invasion of bladder cancer cells - Chenye Tang; Yuntao Wu; Xiao Wang; Kean Chen; Zhiling Tang; Xiao Guo (Human Cell, 2021)
MAFG-AS1 is an oncogenic lncRNA in multiple types of cancer. However, its role in bladder cancer (BC) remains unclear. The present study aimed to investigate the function of MAFG-AS1 in BC. BC and paired non-tumor tissues were collected. Two BC cell lines HT01197 and HT-1376 were used. Dual luciferase activity assay, RT-qPCR, western blot, CCK-8, transwell invasion assay, and wound healing assay were performed. We found that MAFG-AS1 was significantly up-regulated in BC tissues and predicted a poor survival rate. MAFG-AS1 interacted with miR-125b-5p. However, the expression levels of MAFG‑AS1 and miR-125b-5p were not obviously correlated in BC tissues, and MAFG‑AS1 and miR-125b-5p did not regulate the expression of each other. Interestingly, we found that SphK1, a downstream target of miR-125b-5p, was negatively correlated with miR-125b-5p, while it was positively correlated with MAFG-AS1 across BC tissues. In addition, overexpression of MAFG‑AS1 upregulated the expression of SphK1 in BC cells, and attenuated the inhibitory effects of miR-125b-5p on the expression of SphK1. Functional assays showed that overexpression of MAFG‑AS1 promoted BC cell proliferation, migration, and invasion, while its effects were attenuated by overexpression of miR-125b-5p. Moreover, overexpression of miR-125b-5p inhibited BC cell proliferation, migration, and invasion, while its effects were alleviated by overexpression of SphK1. Taken together, our findings demonstrated that MAFG-AS1 has an oncogenic role in BC by regulating the miR-125b-5p/SphK1 axis. MAFG-AS1 might serve as a good diagnostic marker and a potential therapeutic target of BC.

Upregulation of long noncoding RNA XIST has anticancer effects on ovarian cancer through sponging miR-106a - Ting Guo; Donglan Yuan; Wei Zhang; Dandan Zhu; Aifang Xiao; Guangyao Mao; Wenjuan Jiang; Mei Lin; Jun Wang (Human Cell, 2021)

Comprehensive upstream and downstream regulatory analyses identify miR-675-3p as a potential prognostic biomarker in melanoma - Cai-Chou Zhao; Hao Guo; Ying Wang; Jiu-Hong Li (Human Cell, 2021)
This study assessed miR-675-3p-related regulatory mechanisms in melanoma and the clinical relevance of such regulatory activities. We downloaded miRNA mature strand expression RNA-Seq, phenotypic, and DNA methylation data pertaining to the TCGA Melanoma cohort. Differentially expressed miRNAs (DEMs) between metastatic and primary melanoma patient tissues were then identified, and miR-675-3p expression in melanoma patient peripheral blood was confirmed using the GSE20994 GEO dataset, while its expression in melanoma cell lines was evaluated via qRT-RCR. The clinical and prognostic implications of miR-675-3p in melanoma were assessed, and miR-675-3p target genes were identified using bioinformatics tools. Functional roles of this miRNA were explored via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. We identified 3 and 22 miRNAs that were up- and downregulated, respectively, in metastatic melanoma samples relative to primary melanoma samples. Upregulation of miR-675-3p was associated with poorer overall patient survival, tumor histologic grade, and Clark's level. Consistently, miR-675-3p was also overexpressed in the peripheral blood of melanoma patients relative to healthy controls, and in melanoma cell lines relative to control cells. Gene regulatory networks indicated that 32 transcription factors control miR-675-3p expression, and that it, in turn, regulates 10 target genes. KEGG analyses indicated that these genes were associated with cell cycle, transcriptional misregulation in cancer, TGF-beta signaling, and HIF-1 signaling pathways. Gain-of-function assays revealed that miR-675-3p could promote cell proliferation via accelerating cell cycle progression. Western blotting results indicated that miR-675-3p could active TGF-beta and HIF-1 signaling. Through upstream and downstream analyses of miR-675-3p-related regulatory activity, we confirmed that this miRNA participates in key melanoma-related processes and offers value as a prognostic biomarker in melanoma patients.

LncRNA NEAT1 regulates the proliferation and production of the inflammatory cytokines in rheumatoid arthritis fibroblast-like synoviocytes by targeting miR-204-5p - Jianwei Xiao; Rongsheng Wang; Weijian Zhou; Xu Cai; Zhizhong Ye (Human Cell, 2021)

PIWIL1 interacting RNA piR-017061 inhibits pancreatic cancer growth via regulating EFNA5 - Jing Xie; Shen Xing; Bo-Yong Shen; Hai-Tao Chen; Bin Sun; Zheng-Ting Wang; Jing-Wang Wang; Xiong-Xiong Lu (Human Cell, 2021)

Long noncoding RNA NBAT1 suppresses hepatocellular carcinoma progression via competitively associating with IGF2BP1 and decreasing c-Myc expression - Ling Wei; Mengzhi Ling; Song Yang; Yunqian Xie; Changjiang Liu; Wenyi Yi (Human Cell, 2021)

Carcinostatic effects of alkanoyl ascorbate plus platinum nano-colloid and stabilization of the esterolytically resultant ascorbate by hydrogen - Shinya Kato; Yasukazu Saitoh; Nobuhiko Miwa (Human Cell, 2021)

Identification of biomarkers for drug-resistant endometriosis using clinical proteomics - Yoichi Kobayashi; Toshiyuki Fukutomi; Shizuka Mita; Momoe Watanabe; Atsushi Suzuki (Human Cell, 2021)

A positive feedback loop between AlkB homolog 5 and miR-193a-3p promotes growth and metastasis in esophageal squamous cell carcinoma - Jinliang Xue; Peng Xiao; Xiangdong Yu; Xiao Zhang (Human Cell, 2021)

Establishment and characterization of NCC-PLPS1-C1, a novel patient-derived cell line of pleomorphic liposarcoma - Rei Noguchi; Yuki Yoshimatsu; Takuya Ono; Akane Sei; Kaoru Hirabayashi; Iwao Ozawa; Kazutaka Kikuta; Tadashi Kondo (Human Cell, 2021)

MiR-520a-3p inhibits malignant progression of epithelial ovarian cancer by targeting SUV39H1 expression - Jingwei Li; Wei Shao; Junhong Zhao (Human Cell, 2021)

Establishment of a bladder cancer cell line expressing both mesenchymal and epithelial lineage-associated markers - Mohammadrasul Zareinejad; Zahra Faghih; Ali Ariafar; Akbar Safaei; Abbas Ghaderi (Human Cell, 2021)

Establishment and characterization of NCC-MLPS1-C1: a novel patient-derived cell line of myxoid liposarcoma - Rei Noguchi; Yuki Yoshimatsu; Akane Sei; Kaoru Hirabayashi; Iwao Ozawa; Kazutaka Kikuta; Tadashi Kondo (Human Cell, 2021)

Circ0001320 inhibits lung cancer cell growth and invasion by regulating TNFAIP1 and TPM1 expression through sponging miR-558 - Yong Mao; Jia-xi He; Mei Zhu; Yong-quan Dong; Jian-xing He (Human Cell, 2021)

Analysis of microRNA regulating cell cycle-related tumor suppressor genes in endometrial cancer patients - Łukasz Witek; Tomasz Janikowski; Iwona Gabriel; Piotr Bodzek; Anita Olejek (Human Cell, 2021)
Endometrial cancer remains the most common malignancy of the female genital system in developed countries. Tumor suppressor genes are responsible for controlling the cells fate in the cell cycle and preventing cancerogenesis. Gene expression affects cancer progression and is modulated by microRNAs defined as both tumor suppressors and oncogenes. These molecules indirectly regulate multiple processes like cell proliferation, differentiation and apoptosis. The aim of this study was to analyze miRNAs expression that can regulate the activity of tumor suppressor genes related to the cell cycle in patients with endometrioid endometrial cancer. The study group consisted of 12 samples that met the inclusion criteria from a total of 48 obtained. The 12 samples were used to analyze microRNA expression. Complementary miRNAs were identified using TargetScan Database and statistical analysis. MicroRNAs were determined for the tumor suppressor genes: CYR61, WT1, TSPYL5, HNRNPA0, BCL2L1 and BAK1. All the miRNAs were complementary to the described target genes based on TargetScan Database. There were five miRNAs differentially expressed that can regulate tumor suppressor genes related to the cell cycle. The distinguished miRNAs: mir-340-3p, mir-1236-5p, mir-874-3p, mir-873-5p.2 and mir-548-5p were differentially expressed in endometrial cancer in comparison to the control. Among the distinguished miRNAs, the most promising is mir-874-3p, which may have an important role in endometrial adenocarcinoma proliferation.

Downregulating long non-coding RNA PVT1 expression inhibited the viability, migration and phenotypic switch of PDGF-BB-treated human aortic smooth muscle cells via targeting miR-27b-3p - Shouming Li; Xin Zhao; Shaopeng Cheng; Jialiang Li; Xiao Bai; Xiangbin Meng (Human Cell, 2021)

Forkhead box K1 facilitates growth of papillary thyroid carcinoma cells by regulating connective tissue growth factor expression - Hongwei Xu; Yang Liu; Zheng Liu; Xiaoming Wang; Xiubo Lu (Human Cell, 2021)

Osteogenic differentiation of mesenchymal stem cells on the bimodal polymer polyurethane/polyacrylonitrile containing cellulose phosphate nanowhisker - Arash Padash; Raheleh Halabian; Ali Salimi; Negar Motakef Kazemi; Mohsen Shahrousvand (Human Cell, 2021)

Correction to: RCC2 contributes to tumor invasion and chemoresistance to cisplatin in hepatocellular carcinoma - Qingmin Chen; Peiqiang Jiang; Baoxing Jia; Yahui Liu; Ze Zhang (Human Cell, 2021)

ERO1α mediates endoplasmic reticulum stress-induced apoptosis via microRNA-101/EZH2 axis in colon cancer RKO and HT-29 cells - Guoqin Wang; Jiangqiong Han; Gaowei Wang; Xuesong Wu; Youguang Huang; Min Wu; Yunlan Chen (Human Cell, 2021)

Morphological and biochemical changes in the pancreas associated with acute systemic hypoxia - Fumiya Morioka; Naoto Tani; Tomoya Ikeda; Tatsuya Hirokawa; Kei Ikeda; Alissa Shida; Yayoi Aoki; Takaki Ishikawa (Human Cell, 2021)
This study aimed to investigate the changes associated with acute systemic hypoxia in the endocrine system, particularly in pancreatic tissues. The investigation was based on macroscopic, pathohistological, biochemical, and molecular biological findings in cell lines and human cadavers. The results showed that cases of death due to asphyxia more frequently showed severe subcapsular/interstitial hemorrhage versus the other causes of death. Histological examination showed that asphyxia cases were associated with severe morphological changes. Although measured insulin levels in the asphyxia were higher compared to other causes of death, no differences were noted for the glucagon and amylase levels with regard to the cause of death. Increased blood insulin levels were not associated with macro- and micromorphological changes, and did not show any association with glucose or cortisol levels. The experiment conducted under hypoxic conditions in cultured cells demonstrated that insulin mRNA expression and insulin protein levels peaked at 10 min after hypoxia exposure. However, there were no changes in either the amylase mRNA or protein levels. Corticosterone level peaked at 120 min after exposure to hypoxic conditions. Overall, acute systemic hypoxic conditions can directly affect the mechanisms involved in pancreatic insulin secretion.

Both variants of A1CF and BAZ1B genes are associated with gout susceptibility: a replication study and meta-analysis in a Japanese population - Makoto Kawaguchi; Akiyoshi Nakayama; Yuka Aoyagi; Takahiro Nakamura; Seiko Shimizu; Yusuke Kawamura; Mikiya Takao; Takashi Tamura; Asahi Hishida; Mako Nagayoshi; Mitsuo Nagase; Keiko Ooyama; et al. (Human Cell, 2021)
Gout is a common type of acute arthritis that results from elevated serum uric acid (SUA) levels. Recent genome-wide association studies (GWASs) have revealed several novel single nucleotide polymorphism (SNPs) associated with SUA levels. Of these, rs10821905 of A1CF and rs1178977 of BAZ1B showed the greatest and the second greatest significant effect size for increasing SUA level in the Japanese population, but their association with gout is not clear. We examined their association with gout using 1411 clinically-defined Japanese gout patients and 1285 controls, and meta-analyzed our previous gout GWAS data to investigate any association with gout. Replication studies revealed both SNPs to be significantly associated with gout ( P = 0.0366, odds ratio [OR] with 95% confidence interval [CI]: 1.30 [1.02–1.68] for rs10821905 of A1CF , P = 6.49 × 10 –3 , OR with 95% CI: 1.29 [1.07–1.55] for rs1178977 of BAZ1B ). Meta-analysis also revealed a significant association with gout in both SNPs ( P meta = 3.16 × 10 –4 , OR with 95% CI: 1.39 [1.17–1.66] for rs10821905 of A1CF , P meta = 7.28 × 10 –5 , OR with 95% CI 1.32 [1.15–1.51] for rs1178977 of BAZ1B ). This study shows the first known association between SNPs of A1CF , BAZ1B and clinically-defined gout cases in Japanese. Our results also suggest a shared physiological/pathophysiological background between several populations, including Japanese, for both SUA increase and gout susceptibility. Our findings will not only assist the elucidation of the pathophysiology of gout and hyperuricemia, but also suggest new molecular targets.

StarD13 differentially regulates migration and invasion in prostate cancer cells - Leila Jaafar; Isabelle Fakhoury; Sahar Saab; Layal El-Hajjar; Wassim Abou-Kheir; Mirvat El-Sibai (Human Cell, 2021)

Hypoxia/reoxygenation-induced upregulation of miRNA-542-5p aggravated cardiomyocyte injury by repressing autophagy - Fei Wang; Xin Min; Shan-you Hu; Da-li You; Ting-ting Jiang; Li Wang; Xiao Wu (Human Cell, 2021)

Human Angiotensin-Converting Enzyme may be under malaria selection pressure: a need to explore - Aparna Tiwari; Auley De; Veena Pande; Abhinav Sinha (Human Cell, 2021)

Correction to: Enzyme-digested Colla Corii Asini (E’jiao) accelerates wound healing and prevents ultraviolet A-induced collagen synthesis decline and wrinkle formation in three-dimensional skin equivalents - Li Xiao; Feng Liao; Yumei Fan; Nobuhiko Miwa (Human Cell, 2021)

circRNA circ-MYBL2 is a novel tumor suppressor and potential biomarker in multiple myeloma - Shanshan Yu; Limei Ai; Wei Wei; Jing Pan (Human Cell, 2021)

LncRNA TUG1 regulates the balance of HuR and miR-29b-3p and inhibits intestinal epithelial cell apoptosis in a mouse model of ulcerative colitis - Yuxi Tian; Ying Wang; Fujun Li; Junwen Yang; Yan Xu; Miao Ouyang (Human Cell, 2021)

Identification of an epigenetic signature in human induced pluripotent stem cells using a linear machine learning model - Koichiro Nishino; Ken Takasawa; Kohji Okamura; Yoshikazu Arai; Asato Sekiya; Hidenori Akutsu; Akihiro Umezawa (Human Cell, 2021)
The use of human induced pluripotent stem cells (iPSCs), used as an alternative to human embryonic stem cells (ESCs), is a potential solution to challenges, such as immune rejection, and does not involve the ethical issues concerning the use of ESCs in regenerative medicine, thereby enabling developments in biological research. However, comparative analyses from previous studies have not indicated any specific feature that distinguishes iPSCs from ESCs. Therefore, in this study, we established a linear classification-based learning model to distinguish among ESCs, iPSCs, embryonal carcinoma cells (ECCs), and somatic cells on the basis of their DNA methylation profiles. The highest accuracy achieved by the learned models in identifying the cell type was 94.23%. In addition, the epigenetic signature of iPSCs, which is distinct from that of ESCs, was identified by component analysis of the learned models. The iPSC-specific regions with methylation fluctuations were abundant on chromosomes 7, 8, 12, and 22. The method developed in this study can be utilized with comprehensive data and widely applied to many aspects of molecular biology research.

LncRNA NEAT1 regulates chondrocyte proliferation and apoptosis via targeting miR-543/PLA2G4A axis - Peng Xiao; Xu Zhu; Jinpeng Sun; Yuhang Zhang; Weijian Qiu; Jianqiang Li; Xuejian Wu (Human Cell, 2021)

Yin Yang 1-induced LINC00667 up-regulates pyruvate dehydrogenase kinase 1 to promote proliferation, migration and invasion of cholangiocarcinoma cells by sponging miR-200c-3p - Jinglin Li; Canghai Guan; Zengtao Hu; Lang Liu; Zhilei Su; Pengcheng Kang; Xingming Jiang; Yunfu Cui (Human Cell, 2021)

Umbilical cord: an allogenic tissue for potential treatment of COVID-19 - Hugo C. Rodriguez; Manu Gupta; Emilio Cavazos-Escobar; Saadiq F. El-Amin; Ashim Gupta (Human Cell, 2021)

Amniotic fluid mesenchymal stem cells repair mouse corneal cold injury by promoting mRNA N4-acetylcytidine modification and ETV4/JUN/CCND2 signal axis activation - Xinfeng Fei; Yuying Cai; Feng Lin; Yongyi Huang; Te Liu; Yan Liu (Human Cell, 2021)
Severe corneal injury is one of the main causes of loss of visual function. Mesenchymal stem cells (MSCs) have the ability to repair damaged cells in vivo. The present study aimed to explore whether MSCs could function as a cell therapy tool to replace traditional methods to treat corneal injury. CD44 + /CD105 + mesenchymal stem cells isolated from mouse amniotic fluid (mAF-MSCs) were injected into mice after cryoinjury to induce corneal endothelial cell injury. Histopathological assays indicated that mAF-MSCs could promote the growth of corneal epithelial cells, reduce keratitis, and repair the corneal damage caused by low temperature. cDNA microarray analysis revealed that the mAF-MSCs affected the expression patterns of mRNAs related to cell proliferation and differentiation pathways in the mice after transplantation. The results of quantitative real-time PCR and western blotting revealed that NAT12, NAT10, and the ETV4/JUN/CCND2 signaling axis were elevated significantly in the mAF-MSC-transplantation group, compared with those in the phosphate-buffered saline-treated groups. High performance liquid chromatography–mass spectroscopy results revealed that mAF-MSCs could promote mRNA N4-acetylcytidine (ac4C) modification and high expression of N -acetyltransferase in the eyeballs. RNA immunoprecipitation-PCR results showed that a specific product comprising Vegfa , Klf4 , Ccnd2 , Jun , and Etv4 mRNA specific coding region sites could be amplified using PCR from complexes formed in mAF-MSC-transplanted samples cross-linked with anti-ac4C antibodies. Thus, mouse amniotic fluid MSCs could repair the mouse corneal cold injury by promoting the ETV4/JUN/CCND2 signal axis activation and improving its stability by stimulating N4-acetylcytidine modification of their mRNAs.

Establishment and characterization of NCC-LMS2-C1—a novel patient-derived cancer cell line of leiomyosarcoma - Rei Noguchi; Yuki Yoshimatsu; Takuya Ono; Akane Sei; Kaoru Hirabayashi; Iwao Ozawa; Kazutaka Kikuta; Tadashi Kondo (Human Cell, 2021)

Drug screening with a novel tumor-derived cell line identified alternative therapeutic options for patients with atypical teratoid/rhabdoid tumor - Yoshiko Nakano; Mutsumi Takadera; Makoto Miyazaki; Zhiwei Qiao; Kosei Nakajima; Rei Noguchi; Rieko Oyama; Yui Kimura; Yuki Okuhiro; Kai Yamasaki; Noritsugu Kunihiro; Hiroko Fukushima; et al. (Human Cell, 2021)

Knockdown of hsa_circ_0037658 inhibits the progression of osteoarthritis via inducing autophagy - Cong Sui; Debao Liu; Yukang Que; Shenglin Xu; Yong Hu (Human Cell, 2021)

Activation of autophagy following [HuArgI (Co)-PEG5000]-induced arginine deprivation mediates cell death in colon cancer cells - Mirna Swayden; Amira Bekdash; Isabelle Fakhoury; Oula El-Atat; Jamila Borjac-Natour; Mirvat El-Sibai; Ralph J. Abi-Habib (Human Cell, 2021)

The lncRNA PVT1 promotes invasive growth of lung adenocarcinoma cells by targeting miR-378c to regulate SLC2A1 expression - Hongwei Xia; Zhiqiang Zhang; Jun Yuan; Qingling Niu (Human Cell, 2021)