Identification and validation of a novel long non-coding RNA (LINC01465) in ovarian cancer - Shika Hanif Malgundkar; Nada Abdullah Hassan; Hala Al Badi; Ishita Gupta; Ikram A. Burney; Zainab Al Hashami; Hamida Al Barwani; Hamad Al Riyami; Moza Al Kalbani; Ritu Lakhtakia; Aikou Okamoto; Yahya Tamimi (Human Cell, 2022)

Knockdown of lncRNA-ASLNC12002 alleviates epithelial–mesenchymal transition of type II alveolar epithelial cells in sepsis-induced acute respiratory distress syndrome - Kaixuan Feng; Weifeng Huang; Jiawei Shang; Feng Ping; Qin Tan; Wei Wang; Yingchuan Li; Yongmei Cao (Human Cell, 2022)
Patients with sepsis-induced acute respiratory distress syndrome (ARDS) have higher mortality and poor prognosis than pneumonia-induced ARDS. Pulmonary fibrosis is an irreversible accumulation of connective tissue in the interstitium of the lung and closely associated with the epithelial–mesenchymal transition (EMT) of type II alveolar epithelial cells (AECIIs). Therefore, it is undoubtedly worth studying whether the EMT of AECIIs in sepsis-induced ARDS patients is different from that in patients with pneumonia-induced ARDS in the regulatory mechanism. Here, we will report for the first time that an lncRNA-ASLNC12002 is highly expressed in AECIIs of patients with sepsis-induced pneumonia and promotes EMT in AECIIs. The research results showed that the expression of ASLNC12002 in AECIIs derived from patients with sepsis-induced ARDS is significantly higher than that in normal people and pneumonia-induced ARDS patients. Mechanism research showed that ASLNC12002 can cause the inactivation of the anti-EMT pathway NR2F2/miR128-3p/Snail1 by acting as the sponge of miR128-3p. Functional experiments showed that targeted silencing of ASLNC12002 could effectively inhibit EMT progression in AECIIs of patients with sepsis-induced pneumonia by restoring NR2F2/miR128-3p/Snail1 pathway. In a word, our study shows for the first time that the inactivation of NR2F2/miR128-3p/Snail1 pathway caused by the enhanced expression of ASLNC12002 is the direct reason why AECIIs in sepsis-induced ARDS patients are prone to get EMT progress. ASLNC12002 has the potential to become a biological target for the prevention and treatment of pulmonary fibrosis in patients with sepsis-induced ARDS. At the same time, the expectation that ASLNC12002 and its related products may be used as clinical markers for the evaluation of early pulmonary fibrosis in ARDS patients should not be ignored.

Accelerated T-cell exhaustion: its potential role in autoimmune disease and hyperinflammatory disease pathogenesis - Kevin Roe (Human Cell, 2022)

Ex vivo chemosensitivity assay using primary ovarian cancer organoids for predicting clinical response and screening effective drugs - Yu Ito; Jumpei Kondo; Masamune Masuda; Shinya Matsuzaki; Kunishige Onuma; Mizuki Kanda; Yuko Watanabe; Hitomi Sakaguchi; Kiyoshi Yoshino; Yutaka Ueda; Shoji Kamiura; Tadashi Kimura; et al. (Human Cell, 2022)

Retraction Note: LINC00665/miR-9-5p/ATF1 is a novel axis involved in the progression of colorectal cancer - Xuhong Zhao; Wenhao Weng; Yin Long; Weijie Pan; Zhi Li; Fenyong Sun (Human Cell, 2022)

The TRIM family as a potential target for nonalcoholic fatty liver disease - Yuanbin Liu; Mingkai Chen (Human Cell, 2022)

Heterogeneous expression and role of receptor tyrosine kinase-like orphan receptor 2 (ROR2) in small cell lung cancer - Mune Sanada; Masaya Yamazaki; Tatsuya Yamada; Kosuke Fujino; Shinji Kudoh; Yuki Tenjin; Haruki Saito; Noritaka Kudo; Younosuke Sato; Akira Matsuo; Makoto Suzuki; Takaaki Ito (Human Cell, 2022)

Novel CSF1R-positive tenosynovial giant cell tumor cell lines and their pexidartinib (PLX3397) and sotuletinib (BLZ945)-induced apoptosis - Suyanee Thongchot; Supani Duangkaew; Wasan Yotchai; Sorranart Maungsomboon; Rapin Phimolsarnti; Apichat Asavamongkolkul; Peti Thuwajit; Chanitra Thuwajit; Chandhanarat Chandhanayingyong (Human Cell, 2022)
Tenosynovial giant cell tumor (TGCT) is a mesenchymal tumor derived from the synovium of the tendon sheath and joints, most frequently in the large joints. The standard of care for TGCTs is surgical resection. A new targeting approach for treating TGCTs has emerged from studies on the role of the CSF1/CSF1 receptor (CSF1R) in controlling cell survival and proliferation during the pathogenesis of TGCTs. We established four novel cell lines isolated from the primary tumor tissues of patients with TGCTs. The cell lines were designated Si-TGCT-1, Si-TGCT-2, Si-TGCT-3, and Si-TGCT-4, and the TGCT cells were characterized by CSF1R and CD68. These TGCT cells were then checked for cell proliferation using an MTT assay and three-dimensional spheroid. The responses to pexidartinib (PLX3397) and sotuletinib (BLZ945) were evaluated by two-dimensional MTT assays. All cells were positive for α‑smooth muscle actin (α‑SMA), fibroblast activation protein (FAP), CSF1R, and CD68. Except for Si-TGCT-4, all TGCT cells had high CSF1R expressions. The cells exhibited continuous growth as three-dimensional spheroids formed. Treatment with pexidartinib and sotuletinib inhibited TGCT cell growth and induced cell apoptosis correlated with the CSF1R level. Only Si-TGCT-4 cells demonstrated resistance to the drugs. In addition, the BAX/BCL-2 ratio increased in cells treated with pexidartinib and sotuletinib. With the four novel TGCT cell lines, we have an excellent model for further in vitro and in vivo studies.

DNA repair/recombination protein 54L promotes the progression of lung adenocarcinoma by activating mTORC1 pathway - Changjiang Liu; Wei Ren; Zhixin Zhang; Juanjuan Guan (Human Cell, 2022)

Mitochondria: how eminent in ageing and neurodegenerative disorders? - Chia Bamshad; Nima Najafi-Ghalehlou; Zahra Pourmohammadi-Bejarpasi; Kazuo Tomita; Yoshikazu Kuwahara; Tomoaki Sato; Alireza Feizkhah; Amaneh Mohammadi Roushnadeh; Mehryar Habibi Roudkenar (Human Cell, 2022)

Single-cell and microarray chip analysis revealed the underlying pathogenesis of ulcerative colitis and validated model genes in diagnosis and drug response - Liqing Yang; Haiying Chen; Yunong Yang; Yeling Deng; Qiumin Chen; Baiwei Luo; Keren Chen (Human Cell, 2022)
The morbidity rate of ulcerative colitis (UC) in the world is increasing year by year, recurrent episodes of diarrhea, mucopurulent and bloody stools, and abdominal pain are the main symptoms, reducing the quality of life of the patient and affecting the productivity of the society. In this study, we sought to develop robust diagnostic biomarkers for UC, to uncover potential targets for anti-TNF-ɑ drugs, and to investigate their associated pathway mechanisms. We collected single-cell expression profile data from 9 UC or healthy samples and performed cell annotation and cell communication analysis. Revealing the possible pathogenesis of ulcerative colitis by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) analysis. Based on the disease-related modules obtained from weighted correlation network analysis (WGCNA) analysis, we used Lasso regression analysis and random forest algorithm to identify the genes with the greatest impact on disease (EPB41L3, HSD17B3, NDRG1, PDIA5, TRPV3) and further validated the diagnostic value of the model genes by various means. To further explore the relationship and mechanism between model genes and drug sensitivity, we collected gene expression profiles of 185 UC patients before receiving anti-tumor necrosis factor drugs, and we performed functional analysis based on the results of differential analysis between NR tissues and R tissues, and used single-sample GSEA (ssGSEA) and CIBERSORT algorithms to explore the important role of immune microenvironment on drug sensitivity. The results suggest that our model is not only helpful in aiding diagnosis, but also has implications for predicting drug efficacy; in addition, model genes may influence drug sensitivity by affecting immune cells. We suggest that this study has developed a diagnostic model with higher specificity and sensitivity, and also provides suggestions for clinical administration and drug efficacy prediction.

Repeated controlled ovarian stimulation-induced ovarian and uterine damage in mice through the PI3K/AKT signaling pathway - Shuancheng Zhang; Yucong Ma; Qianqian Zuo; Mengrui Liu; Zhifang Yu; Ying Sun; Cuimiao Song; Hongyan Sun; Guimin Hao; Bulang Gao; Huilan Du; Jingwei Chen (Human Cell, 2022)

Establishment and characterization of NCC-PLPS2-C1: a novel cell line of pleomorphic liposarcoma - Taro Akiyama; Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Julia Osaki; Yuki Adachi; Takuya Ono; Ryuto Tsuchiya; Chiaki Sato; Shintaro Iwata; Naoki Kojima; Akihiko Yoshida; et al. (Human Cell, 2022)

Extracellular vesicles derived from cancer-associated fibroblasts carry tumor-promotive microRNA-1228-3p to enhance the resistance of hepatocellular carcinoma cells to sorafenib - Yijie Zhang; Qi Pan; Zigong Shao (Human Cell, 2022)

TRIM59 promotes steatosis and ferroptosis in non-alcoholic fatty liver disease via enhancing GPX4 ubiquitination - Jingxian Zhang; Haina Xie; Jun Yao; Wenye Jin; Huijie Pan; Zhiqiang Pan; Dongyu Xie; Donghao Xie (Human Cell, 2022)
Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease around the world. However, no specific medicine has been approved for NAFLD treatment. Our study was conducted to explore the role and mechanism of TRIM59 in NAFLD, aiming to provide a novel target for NAFLD treatment. Here, the expression of TRIM family members was detected in 10 mild and severe NAFLD tissues as well as 10 normal tissues. TRIM59 expression was verified in 10 normal tissues and 25 mild and severe NAFLD tissues. Palmitic acid and high-fatty diet were used for the construction of NAFLD models. Oil Red O staining was used to detect the level of steatosis. The content of TNF-α, IL-6, and IL-8 was measured to reflect the level of inflammation. Lipid reactive oxygen species was estimated by flow cytometry. We found that TRIM59 was highly expressed in NAFLD tissues compared with normal liver tissues. The inhibition of TRIM59 could inhibit the steatosis and inflammation in NAFLD, whereas its overexpression exhibited reversed effects. The application of ferroptosis inhibitor, deferoxamine, could markedly ameliorate steatosis and inflammation, which was mediated by overexpressed TRIM59. Besides, TRIM59 was demonstrated to interact with GPX4 and promoted its ubiquitination. The overexpression of GPX4 could significantly reverse the pathogenic effects of TRIM59 in NAFLD. Additionally, the inhibition of TRIM59 appeared to be a promising strategy to ameliorate NAFLD in mice model. In summary, our study revealed that TRIM59 could promote steatosis and ferroptosis in NAFLD via enhancing GPX4 ubiquitination. TRIM59 could be a potential target for NAFLD treatment.

Identification and validation of an m6A-related gene signature to predict prognosis and evaluate immune features of breast cancer - Wenhao Li; Xiaolong Wang; Chen Li; Tong Chen; Xianyong Zhou; Zheng Li; Qifeng Yang (Human Cell, 2022)

METTL14 upregulates TCF1 through m6A mRNA methylation to stimulate osteogenic activity in osteoporosis - Xuepeng Wang; Chunchun Zou; Maoqiang Li; Changju Hou; Wu Jiang; Zhenyu Bian; Liulong Zhu (Human Cell, 2022)

MicroRNA-217 aggravates breast cancer through activation of NF1-mediated HSF1/ATG7 axis and c-Jun/ATF3/MMP13 axis - Weihan Li; Chaojie Yang; Jingjing Li; Xiaolian Li; Peng Zhou (Human Cell, 2022)

Cl-amidine attenuates lipopolysaccharide-induced inflammation in human gingival fibroblasts via the JNK/MAPK, NF-κB, and Nrf2 signalling pathways - Jianxin Du; Ning Wang; Hui Sun; Linlin Zheng; Xiangqin Qi (Human Cell, 2022)

The exploitation of enzyme-based cancer immunotherapy - Gourav Chandan; Adesh K. Saini; Reena Kumari; Sasanka Chakrabarti; Amit Mittal; Anil Kumar Sharma; Reena V. Saini (Human Cell, 2022)

PRMT5 promotes retinoblastoma development - Yu Jiang; Guangying Zheng; Xiantao Sun (Human Cell, 2022)

Panobinostat inhibits breast cancer progression via Vps34-mediated exosomal pathway - Xuan Wang; Xuzhi Yin (Human Cell, 2022)

Exosomal circ_0091741 promotes gastric cancer cell autophagy and chemoresistance via the miR-330-3p/TRIM14/Dvl2/Wnt/β-catenin axis - Yuan Chen; Haofeng Liu; Junwei Zou; Guangxin Cao; Yunfei Li; Chungen Xing; Jindong Wu (Human Cell, 2022)

Establishment, characterization and functional testing of two novel ex vivo extraskeletal myxoid chondrosarcoma (EMC) cell models - Jana Lucia Bangerter; Kim Jannis Harnisch; Yanjiang Chen; Catherine Hagedorn; Lara Planas-Paz; Chantal Pauli (Human Cell, 2022)
Extraskeletal myxoid chondrosarcoma (EMC) is a malignant mesenchymal neoplasm of uncertain differentiation as classified by the WHO Classification of Tumours 2020. Although often associated with pronlonged survival, EMC has high rates of distant recurrences and disease-associated death. EMCs are translocation sarcomas and harbor in > 90% of the cases an NR4A3 rearrangement. The molecular consequences of the NR4A3 gene fusions are not yet fully elucidated as well-characterized ex vivo cell models for EMC are lacking. Patient-derived ex vivo models are important and essential tools for investigating disease mechanisms associated with diseases that are rare, that exhibit poor prognosis and for the identification of potential novel treatment options. We established two novel EMC ex vivo models ( USZ20-EMC1 and USZ22-EMC2 ) for functional testing and research purposes. USZ20-EMC1 and USZ22-EMC2 were established and maintained as sarco-sphere cell models for several months in culture. The cells were molecularly characterized using DNA sequencing and methylation profiling. Both cell models represent their native tumor tissue as confirmed by histomorphology and their molecular profiles, suggesting that native tumor cell function can be recapitulated in the ex vivo models. Using a functional screening approach, novel anti-cancer drug sensitivities including potential synergistic combinations were identified. In conclusion, two novel EMC ex vivo cell models ( USZ20-EMC1 and USZ22-EMC2 ) were successfully established and characterized from native tumor tissues. Both cell models will be useful tools for further investigating disease mechanisms and for answering basic and translational research questions.

A protective erythropoietin evolutionary landscape, NLRP3 inflammasome regulation, and multisystem inflammatory syndrome in children - Konstantinos I. Papadopoulos; Alexandra Papadopoulou; Tar-Choon Aw (Human Cell, 2022)

HOXC8 alleviates high glucose-triggered damage of trophoblast cells during gestational diabetes mellitus via activating TGFβ1-mediated Notch1 pathway - Dan Xu; Chengzhen Gao; Yuanyuan Cao; Biru Xiao (Human Cell, 2022)

Genomic distribution of signal transducer and activator of transcription (STAT) family in colorectal cancer - Yanping Hu; Yifen Shen; Yang Zhao; Ying Tang; Chao Liu; Yongchun Gu; Tao Yang; Yihang Shen (Human Cell, 2022)

Diagnostic utility of immunohistochemistry in concordance with mRNA analysis of PRAME in the stratification of high-risk uveal melanoma patients - Nikhil Kumar; Mithalesh Kumar Singh; Lata Singh; Neiwete Lomi; Rachna Meel; Neelam Pushker; Seema Sen; Seema Kashyap (Human Cell, 2022)

Accelerated T-cell exhaustion: its pathogenesis and potentially severe outcomes - Kevin Roe (Human Cell, 2022)

The ubiquitin ligase RNF2 stabilizes ERα and modulates breast cancer progression - Lei Yuan; Xin Li; Huijie Yang; Huixiang Li (Human Cell, 2022)
Estrogen receptor α (ERα) is the most common clinical marker used for breast cancer prognosis and the classification of breast cancer subtypes. Clinically, patients with estrogen receptor-positive breast cancer can receive endocrine therapy. However, resistance to endocrine therapy has become an urgent clinical problem. A large number of previous studies have proven that posttranslational modification of the estrogen receptor is significantly related to endocrine therapy resistance. RNF2 is a member of the RING finger protein family that functions as an E3 ubiquitin ligase. Several studies have clarified that RNF2 is a critical regulator of ERα transcriptional regulation. In our current study, we identified RNF2 as an important posttranslational modification regulator of the estrogen receptor. RNF2 depletion inhibited breast cancer cell progression and ERα signaling activity. TCGA data analysis indicated that RNF2 was elevated in breast malignancies, while RNF2 depletion could drastically inhibit estrogen response gene expression on a whole-genome scale. TCGA data analysis revealed that RNF2 was positively correlated with ERα target gene expression. Further mechanistic studies showed that RNF2 was mainly localized in the nucleus and associated with ERα. The association increased ERα stability by inhibiting ERα K48-linked polyubiquitination. In conclusion, our study implicates nongenomic regulation by RNF2 on ERα protein stability and suggests that targeting RNF2 could be a promising strategy for breast cancer treatments.

The role of miRNAs from mesenchymal stem/stromal cells-derived extracellular vesicles in neurological disorders - Morteza Jafarinia; Majid Reza Farrokhi; Mazdak Ganjalikhani Hakemi; William C. Cho (Human Cell, 2022)

The correlation of CD70 in immune characteristics and drug therapy of pan-cancer - Yang Yang; Jinghong Chen; Mingyang Feng; Yujiao Wang; Weiting Liao; Qiuji Wu; Feng Wen; Qiu Li (Human Cell, 2022)

Correction to: Establishment and characterization of NCC-GCTB5-C1: a novel cell line of giant cell tumor of bone - Taro Akiyama; Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Suguru Fukushima; Yu Toda; Naoki Kojima; Akihiko Yoshida; Seiji Ohtori; Akira Kawai; et al. (Human Cell, 2022)

Correction to: Establishment and characterization of NCC-MRT1-C1: a novel cell line of malignant rhabdoid tumor - Taro Akiyama; Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Chiaki Sato; Naoki Kojima; Akihiko Yoshida; Akira Kawai; Seiji Ohtori; Tadashi Kondo (Human Cell, 2022)

Amniotic stem cells as a source of regenerative medicine to treat female infertility - Aisha Naeem; Nikita Gupta; Usra Naeem; Mohamed A. Elrayess; Chris Albanese (Human Cell, 2022)
Impaired reproductive health is a worldwide problem that affects the psychological well-being of a society. Despite the technological developments to treat infertility, the global infertility rate is increasing significantly. Many infertility conditions are currently treated using various advanced clinical approaches such as intrauterine semination (IUI), in vitro fertilization (IVF), and intracytoplasmic injection (ICSI). Nonetheless, clinical management of some conditions such as dysfunctional endometrium, premature ovarian failure, and ovarian physiological aging still pose significant challenges. Stem cells based therapeutic strategies have a long-standing history to treat many infertility conditions, but ethical restrictions do not allow the broad-scale utilization of adult mesenchymal stromal/stem cells (MSCs). Easily accessible, placental derived or amniotic stem cells present an invaluable alternative source of non-immunogenic and non-tumorigenic stem cells that possess multilineage potential. Given these characteristics, placental or amniotic stem cells (ASCs) have been investigated for therapeutic purposes to address infertility in the last decade. This study aims to summarize the current standing and progress of human amniotic epithelial stem cells (hAECs), amniotic mesenchymal stem cells (hAMSCs), and amniotic fluid stem cells (hAFSCs) in the field of reproductive medicine. The therapeutic potential of these cells to restore or enhance normal ovarian function and pregnancy outcomes are highlighted in this study.

LncRNA NUTM2A-AS1 aggravates the progression of hepatocellular carcinoma by activating the miR-186-5p/KLF7-mediated Wnt/beta-catenin pathway - Jianwu Long; Longfei Liu; Xuefeng Yang; Xiaojun Zhou; Xianzhou Lu; Lei Qin (Human Cell, 2022)

A workflow pipeline to distinguish the ambiguous umbilical cord bloods of boy–girl twins - Jing Cai; Qian Wan (Human Cell, 2022)

Activation of the kynurenine–aryl hydrocarbon receptor axis impairs the chondrogenic and chondroprotective effects of human umbilical cord-derived mesenchymal stromal cells in osteoarthritis rats - Xinwei Wang; Yingjie Zhao; Susu Li; Yueye Wang; Chengyan Jia; Xuezhi Yang; Siyu Li; Bingjie Zhang; Wei Wei; Yan Chang (Human Cell, 2022)

SARS-CoV-2 and Toxoplasma gondii shared symptoms suggest muscle cells and neurons are their chronic infection reservoirs - Kevin Roe (Human Cell, 2022)

HOXD11 upregulates JAM-A and exerts oncogenic properties via NF-κB signaling pathway in esophageal squamous cell carcinoma - Rong Xiong; Qiongxian Long; Xuqian Zhang; Jun Xu; Yanqun Liu; Li Xiong; Siyun Yang; Gang Feng; Guiqing Song; Kang Liu (Human Cell, 2022)

Hedgehog pathway is negatively regulated during the development of Drosophila melanogaster PheRS-m (Drosophila homologs gene of human FARS2) mutants - Lidangzhi Mo; Rui Li; Chunxia He; Qi Chen; Changwei Xu; Liangliang Shen; Kun Chen; Yuanming Wu (Human Cell, 2022)

The progress in techniques for culturing human limbal epithelial stem cells - Yan Shen; Qihua Le (Human Cell, 2022)

Potentials of lncRNA–miRNA–mRNA networks as biomarkers for laryngeal squamous cell carcinoma - Yan Lv; Yanhua Wang; Zhikai Zhang (Human Cell, 2022)

CircZNF367 suppresses osteogenic differentiation of human bone marrow mesenchymal stromal/stem cells via reducing HuR-mediated mRNA stability of LRP5 - Gengyan Liu; Jia Luo; Zhengguang Wang; Yong Zhou; Yong Li (Human Cell, 2022)

CircMERTK modulates the suppressive capacity of tumor-associated macrophage via targeting IL-10 in colorectal cancer - Mingchen Zhu; Zining Zhu; Pan Jiang; Junyu Zheng; Feng Yan; Jifeng Feng (Human Cell, 2022)

Establishment and characterization of a new Chinese hepatocellular carcinoma cell line, Hep-X1 - Hao Xu; Xin Miao; Changpeng Chai; Huan Tang; Jinjing Hu; Zhenjie Zhao; Wei Luo; Kexiang Zhu; Wence Zhou (Human Cell, 2022)

Immunohistochemical expression of nicotinamide N-methyltransferase in lymph node metastases from cutaneous malignant melanoma - Davide Sartini; Elisa Molinelli; Valentina Pozzi; Roberto Campagna; Eleonora Salvolini; Corrado Rubini; Gaia Goteri; Oriana Simonetti; Anna Campanati; Annamaria Offidani; Monica Emanuelli (Human Cell, 2022)

Pyroptosis: a novel signature to predict prognosis and immunotherapy response in gliomas - Guiying He; Zhimin Chen; Shenghua Zhuo; Jingzhi Tang; Weijie Hao; Kun Yang; Chunshui Yang (Human Cell, 2022)

Correction to: MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1 - Yan Zhou; Yanzhen Han; Zhitao Zhang; Zhe Shi; Liyuan Zhou; Xiaohong Liu; Xiaoyan Jia (Human Cell, 2022)

ELK1 suppresses SYTL1 expression by recruiting HDAC2 in bladder cancer progression - Jiansong Wang; Jianjun Luo; Xuecheng Wu; Zhuo Li (Human Cell, 2022)

The role of PYCR1 in inhibiting 5-fluorouracil-induced ferroptosis and apoptosis through SLC25A10 in colorectal cancer - Borong Zhou; Zhongchao Mai; Ying Ye; Yanan Song; Miao Zhang; Xinlin Yang; Wei Xia; Xiaofeng Qiu (Human Cell, 2022)

Establishment and characterization of NCC-PS1-C1: a novel cell line of pleomorphic sarcoma from a patient after neoadjuvant radiotherapy - Taro Akiyama; Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Jun Sugaya; Eisuke Kobayashi; Akihiko Yoshida; Seiji Ohtori; Akira Kawai; Tadashi Kondo (Human Cell, 2022)

METTL3 promotes m6A hypermethylation of RBM14 via YTHDF1 leading to the progression of hepatocellular carcinoma - Jingyao Hu; Liang Yang; Xueqiang Peng; Minghuan Mao; Xiaodan Liu; Jianbo Song; Hangyu Li; Fu Chen (Human Cell, 2022)

Overexpression of PYGO1 promotes early cardiac lineage development in human umbilical cord mesenchymal stromal/stem cells by activating the Wnt/β-catenin pathway - Jie Shen; Xiushan Wu; Ping Zhu; Jian Zhuang; Bin Qin; Fang Sun; Wuzhou Yuan; Xiongwei Fan; Zhigang Jiang; Fang Li; Yongqing Li; Yuequn Wang; et al. (Human Cell, 2022)

Restoration of vascular endothelial integrity by mesenchymal stromal/stem cells in debilitating virus diseases - Agieshkumar Balakrishna Pillai; Vignesh Mariappan; Aashika Raagavi JeanPierre; S. R. Rao (Human Cell, 2022)

RNF126 contributes to stem cell-like properties and metastasis in hepatocellular carcinoma through ubiquitination and degradation of LKB1 - Jie Huang; Yan Li; Mengyao Zheng; Haiyu He; Dingwei Xu; Daguang Tian (Human Cell, 2022)

Detection of ctDNA SNPs by nested real-time Taqman qPCR: a simulation study - Junbei Xiang; Qian Wan; Linfeng Xie (Human Cell, 2022)

SRGAP2 controls colorectal cancer chemosensitivity via regulation of mitochondrial complex I activity - Yongqin Tang; Guijun Liu; Yanhan Jia; Tao Sun (Human Cell, 2022)

Insulin receptor tyrosine kinase substrate (IRTKS) promotes the tumorigenesis of pancreatic cancer via PI3K/AKT signaling - Yu Lu; Xin-Yuan Zhou; Cheng-Liang Zhou; Jie Liu; Tao Yong; Yong Fan; Chen Wang (Human Cell, 2022)

S1P-S1PR1 signaling switch: a new paradigm of tyrosine phosphorylation - Zeeshan Z. Banday (Human Cell, 2022)

Serine protease inhibitor Kazal type 1 (SPINK1) promotes proliferation, migration, invasion and radiation resistance in rectal cancer patients receiving concurrent chemoradiotherapy: a potential target for precision medicine - Yi-Ting Chen; Tzu-Ting Tseng; Hung-Pei Tsai; Shih-Hsun Kuo; Ming-Yii Huang; Jaw-Yuan Wang; Chee-Yin Chai (Human Cell, 2022)
Serine peptidase inhibitor Kazal type-1 (SPINK1), a trypsin kinase inhibitor, is known to be associated with inflammation and pathogenesis. The aim in this study was to demonstrate the clinicopathological role and progression of SPINK1 in rectal cancer (RC) patients undergoing concurrent chemoradiotherapy (CCRT). Immunohistochemical staining for SPINK1 protein expression in 111 RC cases revealed high SPINK1 expression was significantly associated with perineural invasion and poor CCRT response in pre-CCRT specimens. In addition, multivariable analyses showed that pre-CCRT SPINK1 expression was a significant prognostic marker of both overall and disease-free survival in RC patients receiving pre-operative CCRT; furthermore, in vitro studies demonstrated SPINK1 interacted with EGFR to promote the abilities of proliferation, migration and invasion attenuated by SPINK1 si-RNA via ERK, p38, and JNK pathways. SPINK1 was also found to regulate radio-resistance in CRC cell lines. In conclusion, SPINK1 expression is an independent prognostic marker in patients receiving pre-operative CCRT, and SPINK1 regulates proliferation, migration and invasion via EGFR-downstream ERK, p38 and JNK pathways. The phenotypes of radiosensitivity that could be reversed with attenuation of SPINK1 levels suggest that targeting SPINK1 might offer a strategy for optimal precision medicine.

MicroRNA-149-3p expression correlates with outcomes of adrenocortical tumor patients and affects proliferation and cell cycle progression of H295A adrenocortical cancer cell line - Keteryne Rodrigues da Silva; Luciana Chain Veronez; Carolina Alves Pereira Correa; Régia Caroline Peixoto Lira; Mirella Baroni; Rosane de Paula Silva Queiroz; Sonir Roberto Rauber Antonini; José Andres Yunes; Silvia Regina Brandalise; Luiz Gonzaga Tone; Carlos Alberto Scrideli (Human Cell, 2022)

Retraction Note: Long non-coding RNA VPS9D1-AS1 promotes growth of colon adenocarcinoma by sponging miR-1301-3p and CLDN1 - Wei Liu (Human Cell, 2022)

Long non-coding RNA DUXAP10 exerts oncogenic properties in osteosarcoma by recruiting HuR to enhance SOX18 mRNA stability - Guantong Wang; Qian Zhang; Qinjue Wang; Jing Wang; Lulu Chen; Qiang Sun; Dengshun Miao (Human Cell, 2022)
Recent studies have demonstrated that several long non-coding RNAs (lncRNAs) play an important role in the occurrence and development of osteosarcoma (OS). However, more lncRNAs and their mechanisms in regulating growth and migration of OS cells remain to be investigated. In this study, we identified an lncRNA called DUXAP10 by analysis of GEO data, which was significantly up-regulated in OS tissues and cell lines. Experiments in vitro revealed that lncRNA DUXAP10 promoted proliferation, migration, and invasion of OS cells and inhibited their apoptosis. We also demonstrated that DUXAP10 promoted the formation and growth of OS by tumor formation assay. Furthermore, SOX18 was identified as a critical downstream target of DUXAP10 by transcriptome RNA-seq. Mechanistically, DUXAP10 mainly localized in cytoplasm and could specifically bind to HuR to increase the stability of SOX18 mRNA. Meanwhile, SOX18 knockdown largely reversed increased proliferation of OS cells induced by DUXAP10 overexpression. Findings from this study indicate that lncRNA DUXAP10 can act as an oncogene in osteosarcoma by binding HuR to up-regulate the expression of SOX18 at a post-transcriptional level, which may provide a new target for OS clinical diagnosis and treatment.

SQSTM1/p62 promotes miR-198 loading into extracellular vesicles and its autophagy-related secretion - Xiaojie Yu; Hannah Eischeid-Scholz; Lydia Meder; Vangelis Kondylis; Reinhard Büttner; Margarete Odenthal (Human Cell, 2022)
MicroRNA dysregulation is a hallmark of hepatocellular carcinoma (HCC), leading to tumor growth and metastasis. Previous screening on patient specimens identified miR-198 as the most downregulated miRNA in HCC. Here, we show that miR-198 compensation leads to self-release into extracellular vesicles (EVs). Importantly, the vesicular secretion is mediated by autophagy-related pathway, initiated by sequestration of p62/miR-198 complexes in autophagosome-associated vesicle fractions. miR-198 is selectively recognized and loaded by p62 into autophagosomal fractions, whereas mutated miR-198 forms neither induce autophagy and nor interact with p62. Gain and loss of function experiments, using a CRIPR/Cas knockout (KO) and transgenic site-specific p62 mutants, identified p62 as an essential repressor of cellular miR-198 abundancy. Notably, EVs, harboring miR-198/p62 protein complexes, can be uptaken by cells in the close vicinity, leading to change of gene expression in recipient cells. In conclusion, miR-198 enhances autophagy; conversely autophagic protein p62 reduces the miR-198 levels by sorting into extracellular space. Graphical abstract miR-198 is at first transcribed as primary miRNA, after being processed into single stranded mature miR-198 form, it is transported into cytoplasm ①. By interaction with p62 protein, miR-198 conglomerates and forms a binding complex ②. Since LC3 protein is an interaction partner of p62 protein, hence miR-198 is included into autophagosomes ③. By fusion with multivesicular bodies (MVB), miR-198-binding complex was recruited into amphisomes ④, the latter of which quickly turns into secretory MVB containing intraluminal vesicles⑤. By fusion with cell membrane, intraluminal vesicles were released into extracellular space as EVs ⑥.

Retraction Note: Downregulation of miR-96 suppresses the profibrogenic functions of cardiac fibroblasts induced by angiotensin II and attenuates atrial fibrosis by upregulating KLF13 - Lijie Su; Yili Yao; Wei Song (Human Cell, 2022)

Dental follicle cells show potential for treating Parkinson’s disease through dopaminergic-neuronogenic differentiation - Fei Bi; Jie Xiong; Xue Han; Chao Yang; Xinghan Li; Guoqing Chen; Weihua Guo; Weidong Tian (Human Cell, 2022)

Long non-coding RNA IGFBP7-AS1 accelerates the odontogenic differentiation of stem cells from human exfoliated deciduous teeth by regulating IGFBP7 expression - Dan Wang; Ningxin Zhu; Fei Xie; Man Qin; Yuanyuan Wang (Human Cell, 2022)
Stem cells from human exfoliated deciduous teeth (SHED) are attractive seed cells for dental tissue engineering. We identified the effect of the long noncoding RNA insulin-like growth factor-binding protein 7 antisense RNA 1 (lncRNA IGFBP7-AS1) in vivo and its underlying mechanism during SHED odontogenic differentiation. IGFBP7-AS1 and insulin-like growth factor-binding protein 7 ( IGFBP7 ) were overexpressed using lentiviruses. IGFBP7 expression was knocked down with small interfering RNA. The effect of IGFBP7-AS1 in vivo was confirmed by animal experiments. The effect of IGFBP7 on SHED odontogenic differentiation was assessed with alkaline phosphatase staining, alizarin red S staining, quantitative reverse transcription-PCR, and western blotting. The relationship between IGFBP7-AS1 and IGFBP7 was confirmed by quantitative reverse transcription–PCR and western blotting. IGFBP7-AS1 promoted SHED odontogenesis in vivo, and regulated the expression of the coding gene IGFBP7 positively. Inhibiting IGFBP7 led to suppress SHED odontogenic differentiation while IGFBP7 overexpression had the opposite effect. IGFBP7-AS1 enhanced the stability of IGFBP7 . IGFBP7-AS1 promoted SHED odontogenic differentiation in vivo. The underlying mechanism may involve the enhancement of IGFBP7 stability. This may provide novel potential targets for dental tissue engineering.

Exploring prognostic value and regulation network of PPP1R1A in hepatocellular carcinoma - Xixi Wu; Yin Wang; Mi Yang; Yingqiao Wang; Xiaoqing Wang; Longshan Zhang; Liwei Liao; Nan Li; Mengyuan Mao; Jian Guan; Feng Ye (Human Cell, 2022)

Long non-coding RNA RPL34-AS1 ameliorates oxygen–glucose deprivation-induced neuronal injury via modulating miR-223–3p/IGF1R axis - Xin-ya Wei; Tian-qi Zhang; Rui Suo; You-yang Qu; Yan Chen; Yu-lan Zhu (Human Cell, 2022)

Pirfenidone promotes the levels of exosomal miR-200 to down-regulate ZEB1 and represses the epithelial-mesenchymal transition of non-small cell lung cancer cells - Jingjing Liu; Liming Cao; Yuanyuan Li; Pengbo Deng; Pinhua Pan; Chengping Hu; Huaping Yang (Human Cell, 2022)

The role of lincRNA-p21 in regulating the biology of cancer cells - Yan Huang; Qian Yi; Jianguo Feng; Wei Xie; Wei Sun; Weichao Sun (Human Cell, 2022)

Odd-skipped related 1 plays a tumor suppressor role in ovarian cancer via promoting follistatin-like protein 1 transcription - Zhong Yu; Ling Ouyang (Human Cell, 2022)

PTEN-induced kinase 1 enhances the reparative effects of bone marrow mesenchymal stromal cells on mice with renal ischaemia/reperfusion-induced acute kidney injury - Chenyu Lin; Wen Chen; Yong Han; Yujie Sun; Xiaoqiong Zhao; Yuan Yue; Binyu Li; Wenmei Fan; Tao Zhang; Li Xiao (Human Cell, 2022)
Acute kidney injury (AKI) is a common severe acute syndrome caused by multiple factors and is characterized by a rapid decline in renal function during a short period. Bone marrow mesenchymal stromal cells (BMSCs) are effective in treating AKI. However, the mechanism of their beneficial effects remains unclear. PTEN-induced kinase 1 (PINK1) may play an important role in kidney tissue repair. In this study, we explored the effect of PINK1 overexpression on enhancing BMSC-mediated repair of AKI. In this study, ischaemia/reperfusion-induced AKI (IRI-AKI) in mice and a hypoxia-reoxygenation model in cells were established, and the indices were examined by pathology and immunology experiments. After ischaemia/reperfusion, PINK1 overexpression reduced apoptosis in injured kidney tissue cell, decreased T lymphocyte infiltration, increased macrophage infiltration, and alleviated the inflammatory response. PINK1 relieved the stress response of BMSCs and renal tubular epithelial cells (RTECs), reduced apoptosis, altered the release of inflammatory factors, and reduced the proliferation of peripheral blood mononuclear cells (PBMCs). In conclusion, BMSCs and RTECs undergo stress responses in response to hypoxia, inflammation and other conditions, and overexpressing PINK1 in BMSCs could enhance their ability to resist these stress reactions. Furthermore, PINK1 overexpression can regulate the distribution of immune cells and improve the inflammatory response. The regulation of mitochondrial autophagy during IRI-AKI maintains mitochondrial homeostasis and protects renal function. The results of this study provide new strategies and experimental evidence for BMSC-mediated repair of IRI-AKI.

Establishment and characterization of NCC-MFS6-C1: a novel patient-derived cell line of myxofibrosarcoma - Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Taro Akiyama; Chiaki Sato; Eisuke Kobayashi; Naoki Kojima; Akihiko Yoshida; Akira Kawai; Tadashi Kondo (Human Cell, 2022)

MiR-100-5p inhibits osteogenic differentiation of human bone mesenchymal stromal cells by targeting TMEM135 - Rui Wang; Miao Zhang; Ying Hu; Juan He; Qiao Lin; Nianchun Peng (Human Cell, 2022)

Exosomal microRNA-551b-3p from bone marrow-derived mesenchymal stromal cells inhibits breast cancer progression via regulating TRIM31/Akt signaling - Ziang Yang; Bei Xu; Sheng Wu; Weige Yang; Rongkui Luo; Shengkai Geng; Zhaochen Xin; Wen Jin; Xiong Shen; Xixi Gu; Hongwei Zhang; Hong Wang (Human Cell, 2022)

MiR-223-3p-loaded exosomes from bronchoalveolar lavage fluid promote alveolar macrophage autophagy and reduce acute lung injury by inhibiting the expression of STK39 - Nan He; Haoyu Tan; Xueyu Deng; Lu Shu; Bei Qing; Hengxing Liang (Human Cell, 2022)

FOSL2 deficiency delays nonalcoholic steatohepatitis progression by regulating LY6D-mediated NLRP3 activation - Pei-Xin Hu; Mei-Yan Sheng; Yan-Ping Liu; Chun-Qing Zhang (Human Cell, 2022)

Establishment and characterization of NCC-MRT1-C1: a novel cell line of malignant rhabdoid tumor - Taro Akiyama; Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Chiaki Sato; Naoki Kojima; Akihiko Yoshida; Akira Kawai; Seji Ohtori; Tadashi Kondo (Human Cell, 2022)

Astragaloside IV alleviates senescence of vascular smooth muscle cells through activating Parkin-mediated mitophagy - Huijun Li; Jialin Xu; Yanan Zhang; Lei Hong; Zhijian He; Zhiheng Zeng; Li Zhang (Human Cell, 2022)
Astragaloside IV (AS-IV), as one of the main active components of Astragalus membranaceus , has been reported to have cardiovascular protective effects. However, the role and molecular mechanism of AS-IV in vascular senescence have not been clearly stated. The in vitro aging model was constructed using bleomycin (BLM) in vascular smooth muscle cells (VSMCs). Cell senescence were assessed through Western blotting analysis of aging markers, flow cytometry, and the β-galactosidase (SA-β-Gal) kit. Mitophagy was determined through transmission electron microscopy, TMRM staining, and Western blotting analysis of p62. A model of aging blood vessels was induced by d -gal. The vascular wall thickness of mice was also evaluated by H&E staining. Our data proved that AS-IV plays an anti-senescent role in vitro and in vivo. Results showed that AS-IV effectively improved mitochondrial injury, raised MMP, and mediated mitophagy in BLM-induced senescent VSMCs and d -gal induced aging mice. Parkin expression strengthened AS-IV’s anti-senescent function. In conclusions, AS-IV attenuated BLM-induced VSMC senescence via Parkin to regulate mitophagy. Therefore, AS-IV-mediated Parkin might be a latent therapeutic agent and target for VSMC senescence.

Retraction Note to: Exosomes derived from bone mesenchymal stem cells attenuate myocardial fibrosis both in vivo and in vitro via autophagy activation: the key role of miR-199a-3p/mTOR pathway - Chenrong Fan; Qizeng Wang; Youjin Chen; Tingting Ye; Yuncao Fan (Human Cell, 2022)

Circular RNA circPBX3 promotes cisplatin resistance of ovarian cancer cells via interacting with IGF2BP2 to stabilize ATP7A mRNA expression - Lihua Fu; Dan Zhang; Nuo Yi; Yanjun Cao; Yaxian Wei; Wenjing Wang; Li Li (Human Cell, 2022)

TMEM97 is transcriptionally activated by YY1 and promotes colorectal cancer progression via the GSK-3β/β-catenin signaling pathway - Dong Mao; Xiaowei Zhang; Zhaoping Wang; Guannan Xu; Yun Zhang (Human Cell, 2022)

Adipose stromal vascular fraction: a promising treatment for severe burn injury - Khloud Fakiha (Human Cell, 2022)

Long non-coding RNA tumor protein 73 antisense RNA 1 influences an interaction between lysine demethylase 5A and promoter of tumor protein 73 to enhance the malignancy of colorectal cancer - Zhe Huang; He Wang; Mingli Yang (Human Cell, 2022)

Long intergenic noncoding RNA LINC01287 drives the progression of cervical cancer via regulating miR-513a-5p/SERP1 - Yixiang Hu; Wenyou Zhang; Zheng Liu; Qichang Xing; Renzhu Liu; Qingzi Yan; Wencan Li; Xiang Liu (Human Cell, 2022)

Establishment and characterization of IPS-OGC-C1: a novel induced pluripotent stem cell line from healthy human ovarian granulosa cells - Zhiqiang Wang; Xiaojia Hu; Qiufen He; Jingbo Lai; Ruolang Pan; Jing Zheng; Ye Chen (Human Cell, 2022)

ARPC1A is regulated by STAT3 to inhibit ferroptosis and promote prostate cancer progression - Junpeng Ji; Huibing Li; Wenjun Wang; Bo Yuan; Tianyu Shen (Human Cell, 2022)

Long non‑coding RNA PART1: dual role in cancer - Rui Ran; Chao-Yang Gong; Zhi-Qiang Wang; Wen-Ming Zhou; Shun-Bai Zhang; Yong-Qiang Shi; Chun-Wei Ma; Hai-Hong Zhang (Human Cell, 2022)

Sinomenine alleviates glomerular endothelial permeability by activating the C/EBP-α/claudin-5 signaling pathway - Li Zhang; Junxia Wang (Human Cell, 2022)

RhoGDI1 interacts with PHLDA2, suppresses the proliferation, migration, and invasion of trophoblast cells, and participates in the pathogenesis of preeclampsia - Guiyu Song; Feng Jin (Human Cell, 2022)

Hypoxia‐inducible factor 1 alpha (HIF‐1α) stimulated and P2X7 receptor activated by COVID-19, as a potential therapeutic target and risk factor for epilepsy - Hamidreza Zalpoor; Abdullatif Akbari; Mohsen Nabi-Afjadi; Razieh Forghaniesfidvajani; Chanour Tavakol; Zohreh Barzegar; Farideh Iravanpour; Mahshid Hosseini; Seyed Reza Mousavi; Majid Reza Farrokhi (Human Cell, 2022)

Impaired Fanconi anemia pathway causes DNA hypomethylation in human angiosarcomas - Kangning Zhu; Suofeng Sun; Fengxia Guo; Lan Gao (Human Cell, 2022)

Long non-coding RNA LBX2-AS1 predicts poor survival of colon cancer patients and promotes its progression via regulating miR-627-5p/RAC1/PI3K/AKT pathway - Jing Fang; Junyuan Yang; Hui Chen; Wen Sun; Lingyun Xiang; Jueping Feng (Human Cell, 2022)

Exosomal hsa_circ_0017252 attenuates the development of gastric cancer via inhibiting macrophage M2 polarization - Jin Song; Xiaolong Xu; Shasha He; Ning Wang; Yunjing Bai; Bo Li; Shengsheng Zhang (Human Cell, 2022)

Long non-coding RNA NR2F2-AS1: its expanding oncogenic roles in tumor progression - Shadi Ghorbanzadeh; Navid Poor-Ghassem; Masoomeh Afsa; Mohsen Nikbakht; Kianoosh Malekzadeh (Human Cell, 2022)

Long noncoding RNA TRG-AS1 protects against glucocorticoid-induced osteoporosis in a rat model by regulating miR-802-mediated CAB39/AMPK/SIRT-1/NF-κB axis - Wen Liu; Guojuan Li; Jing Li; Wei Chen (Human Cell, 2022)

The EMT-activator ZEB1 is unrelated to platinum drug resistance in ovarian cancer but is predictive of survival - Sophie Rae; Cathy Spillane; Gordon Blackshields; Stephen F. Madden; Joanne Keenan; Britta Stordal (Human Cell, 2022)
The IGROVCDDP cisplatin-resistant ovarian cancer cell line is an unusual model, as it is also cross-resistant to paclitaxel. IGROVCDDP, therefore, models the resistance phenotype of serous ovarian cancer patients who have failed frontline platinum/taxane chemotherapy. IGROVCDDP has also undergone epithelial-mesenchymal transition (EMT). We aim to determine if alterations in EMT-related genes are related to or independent from the drug-resistance phenotypes. EMT gene and protein markers, invasion, motility and morphology were investigated in IGROVCDDP and its parent drug-sensitive cell line IGROV-1. ZEB1 was investigated by qPCR, Western blotting and siRNA knockdown. ZEB1 was also investigated in publicly available ovarian cancer gene-expression datasets. IGROVCDDP cells have decreased protein levels of epithelial marker E-cadherin (6.18-fold, p = 1.58e−04) and higher levels of mesenchymal markers vimentin (2.47-fold, p = 4.43e−03), N-cadherin (4.35-fold, p = 4.76e−03) and ZEB1 (3.43-fold, p = 0.04). IGROVCDDP have a spindle-like morphology consistent with EMT. Knockdown of ZEB1 in IGROVCDDP does not lead to cisplatin sensitivity but shows a reversal of EMT-gene signalling and an increase in cell circularity. High ZEB1 gene expression (HR = 1.31, n = 2051, p = 1.31e−05) is a marker of poor overall survival in high-grade serous ovarian-cancer patients. In contrast, ZEB1 is not predictive of overall survival in high-grade serous ovarian-cancer patients known to be treated with platinum chemotherapy. The increased expression of ZEB1 in IGROVCDDP appears to be independent of the drug-resistance phenotypes. ZEB1 has the potential to be used as biomarker of overall prognosis in ovarian-cancer patients but not of platinum/taxane chemoresistance.

Lipocalin-2 inhibits pancreatic cancer stemness via the AKT/c-Jun pathway - Peipei Hao; Jiamin Zhang; Shu Fang; Miaomiao Jia; Xian Xian; Sinan Yan; Yunpeng Wang; Qian Ren; Fengming Yue; Huixian Cui (Human Cell, 2022)

Long non-coding RNA TRIM52-AS1 sponges microRNA-577 to facilitate diffuse large B cell lymphoma progression via increasing TRIM52 expression - Fang Zhao; Shucheng Li; Jingjing Liu; Juan Wang; Bo Yang (Human Cell, 2022)

miR-203a-3p-DNMT3B feedback loop facilitates non-small cell lung cancer progression - Pingshan Yang; Dongdong Zhang; Fengli Zhou; Wenyou Chen; Chuang Hu; Duqing Xiao; Songwang Cai (Human Cell, 2022)

Transcriptomic and epigenomic analyses explore the potential role of H3K4me3 in neomycin-induced cochlear Lgr5+ progenitor cell regeneration of hair cells - Xiangyu Ma; Shasha Zhang; Shijie Qin; Jiamin Guo; Jia Yuan; Ruiying Qiang; Shan Zhou; Wei Cao; Jianming Yang; Fei Ma; Renjie Chai (Human Cell, 2022)

Establishment and characterization of NCC-SS5-C1: a novel patient-derived cell line of synovial sarcoma - Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Taro Akiyama; Jun Sugaya; Naoki Kojima; Akihiko Yoshida; Akira Kawai; Tadashi Kondo (Human Cell, 2022)

Flt3-ITD mutated acute myeloid leukemia patients and COVID-19: potential roles of autophagy and HIF-1α in leukemia progression and mortality - Hamidreza Zalpoor; Mahnaz Rezaei; Sheida Yahyazadeh; Mazdak Ganjalikhani-Hakemi (Human Cell, 2022)

Establishment and characterization of two novel patient-derived myxoid liposarcoma cell lines - Rei Noguchi; Yuki Yoshimatsu; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Taro Akiyama; Kaoru Hirabayashi; Iwao Ozawa; Rumi Nakagawa; Kazutaka Kikuta; Tadashi Kondo (Human Cell, 2022)

SESN1, negatively regulated by miR-377-3p, suppresses invasive growth of head and neck squamous cell carcinoma by interaction with SMAD3 - Chi Zhang; Lin Ren; Hongjian Zhang; Shiwen Yang; Miao Deng; Lihong He; Ruoyan Cao; Chuanjiang Zhao; Juan Xia (Human Cell, 2022)

Identification of a seven-gene signature predicting clinical outcome of liver cancer based on tumor mutational burden - Yunlong Cui; Ning Jiang (Human Cell, 2022)

Novel acetylation-related gene signatures for predicting the prognosis of patients with colorectal cancer - Zhuang Jing; Feng Ziwang; Wu Yinhang; Zhou Yani; Chu Jian; Wu Jingwen; Han Shuwen (Human Cell, 2022)

Establishment and characterization of a novel patient-derived cell line of dedifferentiated liposarcoma, NCC-DDLPS6-C1 - Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Taro Akiyama; Rumi Nakagawa; Satoshi Kamio; Kaoru Hirabayashi; Iwao Ozawa; Kazutaka Kikuta; Tadashi Kondo (Human Cell, 2022)

SOCS3 gene silencing does not occur through methylation and mutations in gastric cancer - Atika Al Saqri; Shika Hanif Malgundkar; Fatima Al Kindi; Ishita Gupta; Mansour Al Moundhri; Yahya Tamimi (Human Cell, 2022)

The α5-nAChR/PD-L1 axis facilitates lung adenocarcinoma cell migration and invasion - Ping Zhu; Guiyu Kang; Yang Jiao; Chengzhi Gui; Huiping Fan; Xiangying Li; Yanfei Jia; Lulu Zhang; Xiaoli Ma (Human Cell, 2022)

The inhibitory effects of Dulaglutide on cellular senescence against high glucose in human retinal endothelial cells - Shen Nian; Yajing Mi; Kai Ren; Shanwei Wang; Mingkai Li; Di Yang (Human Cell, 2022)

Construction and comprehensive analysis of a lncRNA–mRNA interactive network to reveal a potential lncRNA for hepatic encephalopathy development - Chihao Zhang; Wei Ni; Yiming Zhu; Jiayun Lin; Hongjie Li; Zhifeng Zhao; Ke Wang; Haizhong Huo; Meng Luo (Human Cell, 2022)

KAT2A affects tumor metabolic reprogramming in colon cancer progression through epigenetic activation of E2F1 - Xiaofeng Han; Jie Chen (Human Cell, 2022)

In memoriam: Yoshihiro Kikuchi (1939–2022) - Nariyoshi Shinomiya; Tsunekazu Kita; Hiroaki Kataoka (Human Cell, 2022)

An efficient simplified method for the generation of corneal epithelial cells from human pluripotent stem cells - Rodi Abdalkader; Ken-ichiro Kamei (Human Cell, 2022)

Serum-derived extracellular vesicles mediate Smad4 expression through shuttling microRNA-27a in the progression of laryngeal squamous cell carcinoma - Yu Shuang; Xiaofeng Yao; Jing Liu; Juntao Niu; Wenyu Guo; Chao Li (Human Cell, 2022)

STK11 overexpression prevents glucocorticoid-induced osteoporosis via activating the AMPK/SIRT1/PGC1α axis - Jiao Xiao; Wenjin Li; Guojuan Li; Jiankai Tan; Na Dong (Human Cell, 2022)

Increased neuropilin-1 expression by COVID-19: a possible cause of long-term neurological complications and progression of primary brain tumors - Hamidreza Zalpoor; Hooriyeh Shapourian; Abdullatif Akbari; Shaghayegh Shahveh; Leila Haghshenas (Human Cell, 2022)

Regulation of osteogenic differentiation by the pro-inflammatory cytokines IL-1β and TNF-α: current conclusions and controversies - Qingyun Mo; Wei Zhang; Aijing Zhu; Ludvig J. Backman; Jialin Chen (Human Cell, 2022)

Factors influencing mesenchymal stromal cells in in vitro cellular models to study retinal pigment epithelial cell rescue - Girish K. Srivastava; David Rodriguez-Crespo; Ivan Fernandez-Bueno; José Carlos Pastor (Human Cell, 2022)

The role of GATA3 in the metastasis of triple-negative breast cancer and high-grade serous ovarian cancer - Amr Ahmed El-Arabey; Mohnad Abdalla (Human Cell, 2022)

Establishment and characterization of a novel patient-derived Ewing sarcoma cell line, NCC-ES2-C1 - Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Taro Akiyama; Rumi Nakagawa; Satoshi Kamio; Kaoru Hirabayashi; Iwao Ozawa; Kazutaka Kikuta; Tadashi Kondo (Human Cell, 2022)

Ultrasound-targeted microbubble destruction-mediated silencing of FBXO11 suppresses development of pancreatic cancer - Jing Xue; Shengjiang Chen; Dongfeng Ge; Xiaozhi Yuan (Human Cell, 2022)

A novel mitochondria-related gene signature for controlling colon cancer cell mitochondrial respiration and proliferation - Zhenyu Zhu; Qingsheng Hou; Bishi Wang; Changhao Li; Luguang Liu; Weipeng Gong; Jie Chai; Hongliang Guo (Human Cell, 2022)

RIG-I acts as a tumor suppressor in melanoma via regulating the activation of the MKK/p38MAPK signaling pathway - Rui Guo; Shun-Yuan Lu; Jin-Xia Ma; Qian-Lan Wang; Lu Zhang; Ling-Yun Tang; Yan Shen; Chun-Ling Shen; Jin-Jin Wang; Li-Ming Lu; Zhu-Gang Wang; Hong-Xin Zhang (Human Cell, 2022)
Studies have indicated that RIG-I may act as a tumor suppressor and participate in the tumorigenesis of some malignant diseases. However, RIG-I induces distinct cellular responses via different downstream signaling pathways depending on the cell type. To investigate the biological function and underlying molecular mechanism of RIG-I in the tumorigenesis of melanoma, we constructed RIG-I knockout, RIG-I -overexpressing B16-F10 and RIG-I knockdown A375 melanoma cell lines, and analyzed the RIG-I-mediated change in the biological behavior of tumor cells in spontaneous and poly (I:C)-induced RIG-I activation. Cell proliferation, cell cycling, apoptosis and migration were detected by CCK-8 assay, BrdU incorporation assay, Annexin V–PI staining assay and Transwell assay, respectively. In vivo tumorigenicity was evaluated by tumor xenograft growth in nude mice and subsequently by Ki67 staining and TUNEL assays. Furthermore, Western blotting was utilized to explore the underlying mechanism of RIG-I in melanoma cells. Our data showed that RIG-I promotes apoptosis and inhibits proliferation by G1 phase cell cycle arrest in the melanoma cell lines. Mechanistically, RIG-I induced the phosphorylation of p38 MAPK and MAPK kinases MKK3 and MKK4. In conclusion, the current study demonstrated that RIG-I suppressed the development of melanoma by regulating the activity of the MKK/p38 MAPK signaling pathway, which is relevant to research on novel therapeutic targets for this malignant disease.

Establishment and characterization of the third non-functional human pancreatic neuroendocrine tumor cell line - Xin Lou; Zeng Ye; Xiaowu Xu; Minglei Jiang; Renquan Lu; Desheng Jing; Wuhu Zhang; Heli Gao; Fei Wang; Yue Zhang; Xuemin Chen; Yi Qin; et al. (Human Cell, 2022)

TRIM36 suppresses cell growth and promotes apoptosis in human esophageal squamous cell carcinoma cells by inhibiting Wnt/β-catenin signaling pathway - Bin Zhao; Gaofeng Qiao; Jianhua Li; Yukun Wang; XiaoDong Li; Hua Zhang; Lu Zhang (Human Cell, 2022)

The Piezo1 ion channel in glaucoma: a new perspective on mechanical stress - Yidan Chen; Ying Su; Feng Wang (Human Cell, 2022)

Retraction Note to: Long non-coding RNA MIAT promotes the growth of melanoma via targeting miR-150 - Lifei Zhu; Yexiao Wang; Chaoying Yang; Yanchang Li; Zhixin Zheng; Liangcai Wu; Hui Zhou (Human Cell, 2022)

Exosomes from human adipose-derived mesenchymal stromal/stem cells accelerate angiogenesis in wound healing: implication of the EGR-1/lncRNA-SENCR/DKC1/VEGF-A axis - Yang Sun; Yikun Ju; Bairong Fang (Human Cell, 2022)

Transcription factor p8 regulates autophagy in response to disulfiram via PI3K/mTOR/p70S6K signaling pathway in pancreatic cancer cells - Zhangyu Yao; Xiang Li; Jun Gao; Yutao Wang; Linmei Xiao; Xinxia Chang; Fangzhou Liu; Zhenqing Feng; Xiao Zhang (Human Cell, 2022)

Central nervous system stimulants promote nerve cell death under continuous hypoxia - Kei Ikeda-Murakami; Tomoya Ikeda; Miho Watanabe; Naoto Tani; Takaki Ishikawa (Human Cell, 2022)

Long noncoding RNA ZBTB40-IT1 regulates bone mass by directing the differentiation of human bone marrow mesenchymal stromal cells via the microRNA-514a-3p/FOXO4 axis - Zhe Shi; Qiang Zhong; Yuhang Chen; Xin Luo (Human Cell, 2022)

ATP-gated P2X7 receptor as a potential target for prostate cancer - Cuicui Qiao; Yiqing Tang; Qianqian Li; Xiaodi Zhu; Xiaoxiang Peng; Ronglan Zhao (Human Cell, 2022)

Establishment and characterization of NCC-GCTB5-C1: a novel cell line of giant cell tumor of bone - Taro Akiyama; Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Takuya Ono; Suguru Fukushima; Yu Toda; Naoki Kojima; Akihiko Yoshida; Seji Ohtori; Akira Kawai; et al. (Human Cell, 2022)

RETRACTED ARTICLE: Overexpression of CCN1 in esophageal squamous cell carcinoma attenuates cell proliferation through amyloid precursor protein without DR6 involvement - Pei Wang; Zhiheng Chang; Jing Meng; Xia Cui; Jianyuan Chai; Tong Dang (Human Cell, 2022)

Retraction Note: Long noncoding RNA LINC00460 promotes the progression of cervical cancer via regulation of the miR-361-3p/Gli1 axis - Fan Li; Weipei Zhu; Zhijie Wang (Human Cell, 2022)

Ephrin (Eph) receptor and downstream signaling pathways: a promising potential targeted therapy for COVID‑19 and associated cancers and diseases - Hamidreza Zalpoor; Abdullatif Akbari; Mohsen Nabi-Afjadi (Human Cell, 2022)

Enzyme-digested Colla Corii Asini (E’jiao) suppresses lipopolysaccharide-induced inflammatory changes in THP-1 macrophages and OP9 adipocytes - Li Xiao; Mai Mochizuki; Yumei Fan; Taka Nakahara; Feng Liao (Human Cell, 2022)

Sex-specific changes in the expression of ER-alpha and androgen receptor with increasing tumor grade in patients with hepatocellular carcinoma - Caglar Berkel; Ercan Cacan (Human Cell, 2022)

UBR3 promotes inflammation and apoptosis via DUSP1/p38 pathway in the nucleus pulposus cells of patients with intervertebral disc degeneration - Zhenhuan Jiang; Qinghua Zhao; Liang Chen; Yifeng Luo; Lei Shen; Zhihong Cao; Qiang Wang (Human Cell, 2022)

Circular RNA circ-PLCD1 functions as a tumor suppressor in non-small cell lung cancer by inactivation of PI3K/AKT signaling pathway - Jiming Si; Jianjun Jin; Jingjing Sai; Xiaoting Liu; Xiao Luo; Zhenqiang Fu; Jing Wang (Human Cell, 2022)

Establishment and characterization of NCC-DDLPS5-C1: a novel patient-derived cell line of dedifferentiated liposarcoma - Yooksil Sin; Yuki Yoshimatsu; Rei Noguchi; Ryuto Tsuchiya; Takuya Ono; Taro Akiyama; Shintaro Iwata; Jun Sugaya; Akihiko Yoshida; Akira Kawai; Tadashi Kondo (Human Cell, 2022)

Influence of exogenous adrenaline on insulin sensitivity under general anesthesia in canine model: a preliminary study - Uno Imaizumi; Takashi Hitosugi; Toshikazu Kobayashi; Kenichi Hirano; Takuji Asano; Yoshihiko Kinoshita; Reiko Yokoyama; Masanori Tsukamoto; Takeshi Yokoyama (Human Cell, 2022)

Long noncoding RNA MIR3142HG accelerates lipopolysaccharide-induced acute lung injury via miR-95-5p/JAK2 axis - Yanqiu Gao; Shuangfeng Li; Rui Dong; Xiaoyan Li (Human Cell, 2022)

Aurora kinase genetic polymorphisms: an association study in Down syndrome and spontaneous abortion - Carolina Monteiro Leite de Castro; Carolina Oliveto Bastos Pereira; Joissy Aprigio; Marcelo A. Costa Lima; Márcia G. Ribeiro; Márcia Rodrigues Amorim (Human Cell, 2022)

A20 alleviated caspase-1-mediated pyroptosis and inflammation stimulated by Porphyromonas gingivalis lipopolysaccharide and nicotine through autophagy enhancement - Hui Tang; Yu Ye; Lu Li; Yi Zhou; Liguang Hou; Shuangshuang Ren; Yan Xu (Human Cell, 2022)

Dexmedetomidine post-conditioning alleviates myocardial ischemia–reperfusion injury in rats by ferroptosis inhibition via SLC7A11/GPX4 axis activation - Peng Yu; Jing Zhang; Yi Ding; Dandan Chen; Haijian Sun; Fenglai Yuan; Siyuan Li; Xiaozhong Li; Pingping Yang; Linghua Fu; Shuchun Yu; Jiru Zhang (Human Cell, 2022)

Long non-coding RNA linc00921 suppresses tumorigenesis and epithelial-to-mesenchymal transition of triple-negative breast cancer via targeting miR-9-5p/LZTS2 axis - Jie Zhang; Lina Zhang; Jianlong Wang; Jing Zhao; Xuelian Zhao; Chunli Zhang; Peng Han; Cuizhi Geng (Human Cell, 2022)
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer. Dysregulation of long non-coding RNAs (lncRNAs) plays crucial roles in the initiation and progression of TNBC. In this study, we analyzed public GEO profiles to verify the key lncRNAs in TNBC. Linc00921 was selected for further study. Low expression of linc00921 was observed in 49 of 95 TNBC tissues. Low expression of linc00921 was correlated with poor postoperative disease-free survival (DFS) and overall survival (OS) of TNBC patients. Overexpression of linc00921 with lentivirus suppressed the proliferation, migration and invasion of TNBC cells. A luciferase reporter assay showed that linc00921 could sponge miR-9-5p in TNBC. Moreover, linc00921 and miR-9-5p occupied the same Argonaute-2 (Ago2) protein in TNBC cells. Leucine zipper tumor suppressor 2 (LZTS2) was recognized as a target gene of miR-9-5p, and thereby a linc00921/miR-9-5p/LZTS2 axis was identified in TNBC cells. Overexpression of linc00921 promoted nuclear export of β-catenin, neutralized its function, and subsequently promoted epithelial-to-mesenchymal transition (EMT) in TNBC. A xenograft tumor mouse model showed that the miR-9-5p inhibitor upregulates LZTS2 expression and induce nuclear export of β-catenin in TNBC. Thus, linc00921 upregulates LZTS2 by sponging miR-9-5p to suppress tumorigenesis and EMT of TNBC. Linc00921/miR-9-5p/LZTS2 axis may be a novel biomarker and therapeutic target for TNBC patients.

Regulation of PTEN and ovarian cancer progression by an E3 ubiquitin ligase RBCK1 - Guanghui Wang; Zi Zhuang; Siyuan Shen; Fan Yang; Zhiyuan Jiang; Ziping Liu; Tianshi Wang; Lei Hua (Human Cell, 2022)

MiR-1260b protects against LPS-induced degenerative changes in nucleus pulposus cells through targeting TCF7L2 - Shijie Chen; Guixia Shi; Jin Zeng; Ping Huang Li; Yi Peng; Zhiyu Ding; Hong Qing Cao; Ruping Zheng; Weiguo Wang (Human Cell, 2022)

Non-coding RNAs in ferroptotic cancer cell death pathway: meet the new masters - Mehdi Rabiee Valashedi; Chia Bamshad; Nima Najafi-Ghalehlou; Amirsadegh Nikoo; Kazuo Tomita; Yoshikazu Kuwahara; Tomoaki Sato; Amaneh Mohammadi Roushandeh; Mehryar Habibi Roudkenar (Human Cell, 2022)

Knockout of GGPPS1 restrains rab37-mediated autophagy in response to ventilator-induced lung injury - Zexu Wang; Meizi Chen; Xia Pan; Li Wang; Cheng Yin; Qiuqi Lin; Jingjing Jiang; Yunlei Zhang; Bing Wan (Human Cell, 2022)

Exosomes: a new way of protecting and regenerating optic nerve after injury - Huazhang Li; Ying Su; Feng Wang; Feng Tao (Human Cell, 2022)

Establishment of a mouse model of pancreatic cancer using human pancreatic cancer cell line S2-013-derived organoid - Chiharu Tanaka; Kaoru Furihata; Seiji Naganuma; Mitsunari Ogasawara; Reiko Yoshioka; Hideki Taniguchi; Mutsuo Furihata; Keisuke Taniuchi (Human Cell, 2022)
A well-established preclinical model of pancreatic cancer needs to be established to facilitate research on new therapeutic targets. Recently established animal models of pancreatic cancer, including patient-derived tumor models and organoid models, are used for pre-clinical drug testing and biomarker discovery. These models have useful characteristics over conventional xenograft mouse models based on cell lines in preclinical studies, but still cannot accurately predict the clinical outcomes of new treatments and have not yet been broadly implemented in research. We employed pancreatic cancer organoid culture methods using the pancreatic cancer cell line S2-013, and performed pathological and immunohistochemical analyses to characterize tumor xenografts obtained from a mouse model implanted with S2-013 cell line-derived organoids. Serum levels of the pancreatic cancer tumor marker CA19-9 were measured by ELISA. We generated human pancreatic cancer organoids using a co-culture of S2-013 cells, human endothelial cells derived from human umbilical vein endothelial cells, and human mesenchymal stem cells, and established a mouse model with subcutaneously transplanted human pancreatic cancer organoids (S2-013-organoid model). Although blood clotting crater-like formation developed in the middle of subcutaneous xenografts in the S2-013-conventional model, created by subcutaneously injecting S2-013 cells into the right flank of nude mice, the size of xenografts in the S2-013-organoid model gradually increased without crater-like formation. Importantly, tumor xenografts obtained from the S2-013-organoid model exhibited a clinical human pancreatic cancer tissue-like cellular morphology, tissue architecture, and polarity, and actively formed cancer stroma containing mature blood vessels with the high expression of the vascular tight junction marker CD31. In subcutaneous xenografts of S2-013-conventional mice, no blood vessel density or widely expanding areas of necrotic regions were present. Consequently, serum levels of CA19-9 in the S2-013-organoid model correlated with tumor volumes. In addition, epithelial–mesenchymal transition, the conversion of epithelial cells to the mesenchymal phenotype, was observed in tumor xenografts of the S2-013-organoid model. The S2-013-organoid model provides tumor xenografts consisting of clinical human pancreatic cancer-like tissue formation with the effective development of vascularized stroma, and may be valuable for facilitating studies on pre-clinical drug testing and biomarker discovery.

USP36 promotes tumor growth of non-small cell lung cancer via increasing KHK-A expression by regulating c-MYC–hnRNPH1/H2 axis - Qian Deng; Maolin Wu; Jing Deng (Human Cell, 2022)

MiR-182-5p inhibits the tumorigenesis of clear cell renal cell carcinoma by repressing UBE2T - Yucai Wu; Cuijian Zhang; Ding Peng; Shiming He; Cong Huang; Jinqin Qian; Weijie Zhu; Ninghan Feng; Yanqing Gong; Xuesong Li; Liqun Zhou (Human Cell, 2022)

Establishment and characterization of NCC-UPS4-C1: a novel cell line of undifferentiated pleomorphic sarcoma from a patient with Li–Fraumeni syndrome - Takuya Ono; Yuki Yoshimatsu; Rei Noguchi; Yooksil Sin; Ryuto Tsuchiya; Taro Akiyama; Jun Sugaya; Suguru Fukushima; Naoki Kojima; Akihiko Yoshida; Akira Kawai; Tadashi Kondo (Human Cell, 2022)

Inducing substances for chondrogenic differentiation of dental pulp stem cells in the conditioned medium of a novel chordoma cell line - Hiroyoshi Kino; Hiroyoshi Akutsu; Hiroshi Ishikawa; Shingo Takano; Shohei Takaoka; Junko Toyomura; Takuma Hara; Eiichi Ishikawa; Yuji Matsumaru; Hiroki Bukawa; Akira Matsumura (Human Cell, 2022)

The promotional effect of microRNA-103a-3p in cervical cancer cells by regulating the ubiquitin ligase FBXW7 function - Li Ren; Jinjin Yang; Xiyan Meng; Junjun Zhang; Yiran Zhang (Human Cell, 2022)
MicroRNAs (miRNAs) have been reported to be involved in the initiation and progression of human tumors including cervical cancer (CC). However, the mechanisms underlying of their actions in CC remain to be fully elucidated. Herein, the differentially expressed miRNAs that were screened based on GSE55940 microarray data retrieved from Gene Expression Omnibus (GEO), and miR-103a-3p was significantly upregulated in CC tissues which was selected as the target miRNA for further research. We also found that high expression of miR-103a-3p was closely associated with histological grades, FIGO stage and distant metastasis as well as reflected poor overall survival. Moreover, miR-103a-3p inhibition decreased the growth capacity of SiHa and HeLa cells by inducing cell apoptosis. And F-box and WD repeat-domain containing protein 7 (FBXW7), a well-known tumor suppressor in many cancer types, was identified as a direct target of miR-103a-3p. It was further found that FBXW7 was significantly downregulated in CC tissues, and it was inversely correlated with miR-103a-3p expression levels. Further investigation demonstrated that FBXW7 upregulation could simulate the roles of miR-103a-3p knockdown in cell viability and apoptosis. Moreover, FBXW7 knockdown efficiently abrogated the influences of CC cells proliferation caused by miR-103a-3p inhibition. Notably, miR-103a-3p could block FBXW7 mediated the downstream transcription factor pathways. Taken together, these findings suggest that miR-103a-3p functions as an oncogene in CC by targeting FBXW7.

Knockdown of UCHL3 inhibits esophageal squamous cell carcinoma progression by reducing CRY2 methylation - Jijun Xue; Jinyuan Yi; Xiaolong Zhu (Human Cell, 2022)

Blockade of ASIC1a inhibits acid-induced rat articular chondrocyte senescence through regulation of autophagy - Yang Yang; Jie Ding; Yong Chen; Ganggang Ma; Xin Wei; Renpeng Zhou; Wei Hu (Human Cell, 2022)

The role of YAP1 in small cell lung cancer - Haruki Saito; Yuki Tenjin; Tatsuya Yamada; Shinji Kudoh; Noritaka Kudo; Mune Sanada; Younosuke Sato; Akira Matsuo; Yorihisa Orita; Takaaki Ito (Human Cell, 2022)

An integrated analysis of single-cell and bulk transcriptomics reveals EFNA1 as a novel prognostic biomarker for cervical cancer - Xiaopeng Shen; Meng Li; Yang Lei; Shan Lu; Sufen Wang; Zhongxian Liu; Chunguang Wang; Yun Zhao; Ao Wang; Chao Bi; Guoping Zhu (Human Cell, 2022)

DNA methylation: a saga of genome maintenance in hematological perspective - Saran Chattopadhyaya; Somnath Ghosal (Human Cell, 2022)

Development of a CAFs-related gene signature to predict survival and drug response in bladder cancer - Zhao Zhang; Zhijuan Liang; Dan Li; Liping Wang; Yuanbin Chen; Ye Liang; Wei Jiao; Haitao Niu (Human Cell, 2022)

Bystander effects induced by the interaction between urothelial cancer cells and irradiated adipose tissue-derived stromal cells in urothelial carcinoma - Maki Kawasaki; Kei Nagase; Shigehisa Aoki; Kazuma Udo; Shohei Tobu; Mihoko Rikitake-Yamamoto; Masaya Kubota; Takayuki Narita; Mitsuru Noguchi (Human Cell, 2022)

A meta-analysis of genome-wide association studies using Japanese and Taiwanese has revealed novel loci associated with gout susceptibility - Shun-Jen Chang; Yu Toyoda; Yusuke Kawamura; Takahiro Nakamura; Masahiro Nakatochi; Akiyoshi Nakayama; Wei-Ting Liao; Seiko Shimizu; Tappei Takada; Kenji Takeuchi; Kenji Wakai; Yongyong Shi; et al. (Human Cell, 2022)

RNA-seq analysis of phagocytic cells from murine epididymal white adipose tissue shows immunosenescence and age-related phosphorus metabolism - Martin Nwadiugwu (Human Cell, 2022)

Activation-induced cell death in CAR-T cell therapy - Tian Huan; Dongfeng Chen; Guodong Liu; Hailing Zhang; Xiaoyan Wang; Zhi Wu; Yan Wu; Qinggang Xu; Feng Yu (Human Cell, 2022)

Deciphering the influence of urinary microbiota on FoxP3+ regulatory T cell infiltration and prognosis in Chinese patients with non-muscle-invasive bladder cancer - Yifeng Qiu; Yubo Gao; Chunxiao Chen; Ming Xie; Pengcheng Huang; Qi Sun; Zhipeng Zhou; Biao Li; Jie Zhao; Peng Wu (Human Cell, 2022)

VPS9D1-AS1 gene rs7206570 polymorphism associated with the clinical stage of colorectal cancer and binding with hsa-miR-361-3p - Xueren Gao; Shulong Zhang; Xiaoting Wang (Human Cell, 2022)

Circular RNA circ_0008934 promotes hepatocellular carcinoma growth and metastasis through modulating miR-1305/TMTC3 axis - Jia-Xi Li; Jin-Jiang Wang; Zhou-Feng Deng; Hao Zheng; Chun-Mei Yang; Ying Yuan; Cheng Yang; Fang-Fang Gu; Wei-Qi Wu; Guang-Lei Qiao; Li-Jun Ma (Human Cell, 2022)

A systematic review on active sites and functions of PIM-1 protein - Youyi Zhao; Aziz ur Rehman Aziz; Hangyu Zhang; Zhengyao Zhang; Na Li; Bo Liu (Human Cell, 2022)

Transplanted neural lineage cells derived from dental pulp stem cells promote peripheral nerve regeneration - Shohei Takaoka; Fumihiko Uchida; Hiroshi Ishikawa; Junko Toyomura; Akihiro Ohyama; Miho Watanabe; Hideaki Matsumura; Aiki Marushima; Seiichiro Iizumi; Satoshi Fukuzawa; Naomi Ishibashi-Kanno; Kenji Yamagata; et al. (Human Cell, 2022)

Shear stress regulates the migration of suspended breast cancer cells by nuclear lamina protein A/C and large tumor suppressor through yes-associated protein - Boyuan Zhao; Mei Tang; Yonggang Lv (Human Cell, 2022)

RBPMS2, as a novel biomarker for predicting lymph node metastasis, guides therapeutic regimens in gastric cancer - Han Zhao; Yuxin Tong; Siwei Pan; Zhendong Qiu; Pengfei Liu; Pengtao Guo (Human Cell, 2022)

RETRACTED ARTICLE: Exosomes derived from bone mesenchymal stem cells attenuate myocardial fibrosis both in vivo and in vitro via autophagy activation: the key role of miR-199a-3p/mTOR pathway - Chenrong Fan; Qizeng Wang; Youjin Chen; Tingting Ye; Yuncao Fan (Human Cell, 2022)

SOX9 contributes to the progression of ductular reaction for the protection from chronic liver injury - Daiki Yoshii; Keita Shimata; Yuji Yokouchi; Yoshihiro Komohara; Hiroko Suda; Masaki Honda; Kenichi Yamamura; Taizo Hibi; Yukihiro Inomata (Human Cell, 2022)

Human placental mesenchymal stromal cell therapy restores the cytokine efflux and insulin signaling in the skeletal muscle of obesity-induced type 2 diabetes rat model - Nagasuryaprasad Kotikalapudi; Samuel Joshua Pragasam Sampath; Sukesh Narayan Sinha; R. Bhonde; Sathish Kumar Mungamuri; Vijayalakshmi Venkatesan (Human Cell, 2022)

Role of aurora kinase B in regulating resistance to paclitaxel in breast cancer cells - Min Liu; Yinan Li; Cui Zhang; Qing Zhang (Human Cell, 2022)
Aurora kinase B (AURKB) is a type of functional kinase with primary functions of participating in cell mitosis, which has been identified to be involved in the occurrence and development of malignant tumors strongly. However, it still remains a controversial with respect to the relationship between the phosphorylation level of AURKB and its function. In our initial research, there was no significant difference in the relative content of AURKB protein between drug-resistant breast cancer cells and wild-type cells; however, its phosphorylation level in drug-resistant cells was significantly higher than that in wild-type cells. Subsequent cell and animal experiments both confirmed the positive correlation between AURKB phosphorylation and drug resistance. Furthermore, PRKCE in the upstream was identified to regulate the phosphorylation of AURKB, which promoted the change of spatial localization of AURKB from nucleus to cytoplasm. Accordingly, phosphorylated AURKB reduced the negative regulation of downstream RAB27B transcription physically, and interacted with RAB27B in cytoplasm to maintain its protein stability. Eventually, it promoted exosome secretion of drug-resistant cells and drug efflux. Using shRNA to knockdown AURKB expression, using hesperadin to inhibit AURKB activity, mutating the AURKB phosphorylation site, or using siRNA as well as BIM to inhibit the activity of the upstream AURKB phosphorylation regulatory protein PRKCE, all of which directly or indirectly reduce AURKB phosphorylation, are effective in reversing PTX resistance in cells. Collectively, this study provides experimental evidence for PRKCE/AURKB/RAB27B axis in regulating the resistance to paclitaxel (PTX) in breast cancer cells, offering a potential intervention target for reversing drug resistance.

Differential microRNA expression profile in blood of children with Down syndrome suggests a role in immunological dysfunction - Joice Matos Biselli; Bruna Lancia Zampieri; Patrícia Matos Biselli-Chicote; Jorge Estefano Santana de Souza; Matheus Carvalho Bürger; Wilson Araújo da Silva Jr; Eny Maria Goloni-Bertollo; Érika Cristina Pavarino (Human Cell, 2022)

The miR-214-3p/c-Ski axis modulates endothelial–mesenchymal transition in human coronary artery endothelial cells in vitro and in mice model in vivo - Juan Wang; Hongjian Li; Zhongying Lv; Xiaomei Luo; Wei Deng; Ting Zou; Yue Zhang; Wanyue Sang; Xuehua Wang (Human Cell, 2022)

The difference in the intracellular Arg/Lys-rich and EHLVY motifs contributes to distinct subcellular distribution of HAI-1 versus HAI-2 - Nanxi Huang; Robert B. Barndt; Dajun D. Lu; Qiaochu Wang; Shih-Ming Huang; Jehng-Kang Wang; Ping-Ying Chang; Chao-Yang Chen; Je-Ming Hu; Hui-Chen Su; Michael D. Johnson; Chen-Yong Lin (Human Cell, 2022)

A four immune-related long noncoding RNAs signature as predictors for cervical cancer - Min Xu; Runjie Zhang; Jin Qiu (Human Cell, 2022)

Overexpression of long noncoding RNA MCM3AP-AS1 promotes osteogenic differentiation of dental pulp stem cells via miR-143-3p/IGFBP5 axis - Changwei Yang; Xuehong Xu; Pingting Lin; Bizhu Luo; Shufang Luo; Honglan Huang; Jianyu Zhu; Meie Huang; Shuhai Peng; Qianju Wu; Lu Yin (Human Cell, 2022)

USP21 regulates Hippo signaling to promote radioresistance by deubiquitinating FOXM1 in cervical cancer - Zhengliang Li; Xiaojing Liu; Haizhou Yu; Shaoping Wang; Shuliang Zhao; Guoxiang Jiang (Human Cell, 2022)

Establishment of an immortalized human erythroid cell line sustaining differentiation potential without inducible gene expression system - Svetlana Soboleva; Ryo Kurita; Naoko Kajitani; Hugo Åkerstrand; Kenichi Miharada (Human Cell, 2022)

Long noncoding RNA SNHG3 promotes malignant phenotypes in cervical cancer cells via association with YAP1 - Hongyu Zhu; Chenyu Zhu; Xiang Feng; Youzhen Luo (Human Cell, 2022)

Serine protease HtrA2/Omi regulates adaptive mitochondrial reprogramming in the brain cortex after ischemia/reperfusion injury via UCP2-SIRT3-PGC1 axis - Hao Meng; Lian-Kun Sun; Jing Su; Wan-Yu Yan; Yao Jin; Xin Luo; Xian-Rui Jiang; Hong-Lei Wang (Human Cell, 2022)

Global miRNA expression of bone marrow mesenchymal stem/stromal cells derived from Fanconi anemia patients - Ilgin Cagnan; Mustafa Keles; Ayse Gokce Keskus; Melike Tombaz; Ozge Burcu Sahan; Fatima Aerts-Kaya; Duygu Uckan-Cetinkaya; Ozlen Konu; Aysen Gunel-Ozcan (Human Cell, 2022)

HSP27 protects against ferroptosis of glioblastoma cells - Fanen Yuan; Qian Sun; Si Zhang; Liguo Ye; Yang Xu; Zhou Xu; Baohui Liu; Shenqi Zhang; Qianxue Chen (Human Cell, 2022)

Evaluation of the programmed death-ligand 1 mRNA expression and immunopositivity and their correlation with survival outcomes in Indian lung cancer patients - Sachin Kumar; Monu Pandey; Ishfaq A. Mir; Abhirup Mukhopadhyay; Surender K. Sharawat; Deepali Jain; Jyoutishman Saikia; Prabhat S. Malik; Sunil Kumar; Anant Mohan (Human Cell, 2022)

Protein tyrosine phosphatase non-receptor type 12 (PTPN12), negatively regulated by miR-106a-5p, suppresses the progression of hepatocellular carcinoma - Zhanqiang Liang; Xingxing Li; Fei Duan; Liming Song; Zhongzhen Wang; Xuemin Li; Pengsheng Yang; Liantao Li (Human Cell, 2022)

Establishment and characterization of NCC-MPNST6-C1: a novel patient-derived cell line of malignant peripheral nerve sheath tumors - Yooksil Sin; Yuki Yoshimatsu; Rei Noguchi; Ryuto Tsuchiya; Takuya Ono; Taro Akiyama; Fumihiko Nakatani; Jun Sugaya; Akihiko Yoshida; Akira Kawai; Tadashi Kondo (Human Cell, 2022)

3D modeling in cancer studies - Oula El Atat; Zahra Farzaneh; Mahsa Pourhamzeh; Fatima Taki; Ralph Abi-Habib; Massoud Vosough; Mirvat El-Sibai (Human Cell, 2022)

Establishment and characterization of the NCC-GCTB4-C1 cell line: a novel patient-derived cell line from giant cell tumor of bone - Takuya Ono; Rei Noguchi; Yuki Yoshimatsu; Ryuto Tsuchiya; Yooksil Sin; Rumi Nakagawa; Kaoru Hirabayashi; Iwao Ozawa; Kazutaka Kikuta; Tadashi Kondo (Human Cell, 2022)

Colorectal cancer promoter methylation alteration affects the expression of glutamate ionotropic receptor AMPA type subunit 4 alternative isoforms potentially relevant in colon tissue - Ana Florencia Vega-Benedetti; Eleonora Loi; Loredana Moi; Angelo Restivo; Francesco Cabras; Simona Deidda; Andrea Pretta; Pina Ziranu; Sandra Orrù; Mario Scartozzi; Luigi Zorcolo; Patrizia Zavattari (Human Cell, 2022)
DNA methylation alterations are early events during tumourigenesis, affecting genes involved in the crosstalk between cells and surroundings in colorectal cancer (CRC). Among these genes, GRIA4 , Glutamate Ionotropic Receptor AMPA Type Subunit 4, displays hypermethylation in the promoter region, and is an early diagnostic biomarker. It is well known that methylation can also affect alternative transcription. The purpose of this study is to evaluate the expression, at transcript and protein level, of GRIA4 main isoforms (the canonical one and a short variant) in 23 CRC and matched normal samples, of which we previously verified the methylation status. We further predicted miRNA/transcript target interactions as a possible post-transcriptional regulation using bioinformatics tools. As expected, downregulation of both variants has been observed in tumours. Interestingly, in contrast to what observed at transcriptional level, the GluR4 protein short isoform displayed higher expression than the canonical one either in normal or tumoural tissues. This may be explained by miRNA specifically targeting the canonical isoform. Our study is the first one that shows the expression of both isoforms in colon tissues. To note, the evident expression of the short isoform suggests a functional role in intestinal cell biology.