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A rapid and sensitive RPA-Cas12a assay for detection of banana bunchy top virus (Babuvirus musae)NARA Subscribed
Banana bunchy top virus (BBTV, Babuvirus musae ) is one of the most damaging viral threats to banana production, yet frontline diagnostics rely on PCR, ELISA or proprietary assays that are poorly suited to low resource deployment or associated with high per test cost. We developed a recombinase polymerase amplification (RPA)-Cas12a assay that achieves high sensitivity using crude extracts with fluorescence or lateral flow read...
Development of Specific Diagnostic Assays for the Eleven Main Viruses Infecting Garlic (Allium sativum)NARA Subscribed
Commercially grown garlic is usually infected by complex mixtures of carla-, poty-, and allexiviruses. Specific detection of these viruses is difficult, particularly as some such as the allexiviruses exhibit serological cross-reactivity, and intraspecific nucleic acid sequence diversity is relatively large. While next generation sequencing shows much promise as a diagnostic method, it is not yet widely used for routine virus s...
Systemic deficits in lipid homeostasis promote aging-associated impairments in B cell progenitor developmentNARA Subscribed
Organismal aging has been associated with diverse metabolic and functional changes across tissues. Within the immune system, key features of physiological hematopoietic cell aging include increased fat deposition in the bone marrow, impaired hematopoietic stem and progenitor cell (HSPC) function, and a propensity towards myeloid differentiation. This shift in lineage bias can lead to pre-malignant bone marrow conditions such a...
Investigating impacts of the mycothiazole chemotype as a chemical probe for the study of mitochondrial function and agingNARA Subscribed
Small molecule inhibitors of the mitochondrial electron transport chain (ETC) hold significant promise to provide valuable insights to the field of mitochondrial research and aging biology. In this study, we investigated two molecules: mycothiazole (MTZ) - from the marine sponge C. mycofijiensis and its more stable semisynthetic analog 8- O -acetylmycothiazole (8-OAc) as potent and selective chemical probes based on their high...
Perennial intermediate wheatgrass accumulates more soil organic carbon than annual winter wheat – a model assessmentNARA Subscribed
Purpose Perennial crops have been suggested as a more sustainable alternative to the currently most common cropping systems. Compared with annual plants, perennial plants produce more biomass and have deeper roots, and are expected to lead to higher soil organic carbon (SOC). This hypothesis, however, has not been well tested for grain crops. Methods Using perennial intermediate wheatgrass (IWG, Thinopyrum intermedium ) and an...
Common, low-frequency, rare, and ultra-rare coding variants contribute to COVID-19 severityNARA Subscribed
The combined impact of common and rare exonic variants in COVID-19 host genetics is currently insufficiently understood. Here, common and rare variants from whole-exome sequencing data of about 4000 SARS-CoV-2-positive individuals were used to define an interpretable machine-learning model for predicting COVID-19 severity. First, variants were converted into separate sets of Boolean features, depending on the absence or the pr...