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Journal of Marine Science and Engineering · 2017 · Vol. 5 · Issue 1 · MDPI
The common practice of counting bacteria using epifluorescence microscopy involves selecting 5–30 random fields of view on a glass slide to calculate the arithmetic mean which is then used to estimate the total bacterial abundance. However, not much is known about the accuracy of the arithmetic mean when it is calculated by selecting random fields of view and its effect on the overall abundance. The aim of this study is to eva...